Click here to switch to the map view.

The map label for this gene is argF [H]

Identifier: 103486844

GI number: 103486844

Start: 1415250

End: 1416173

Strand: Reverse

Name: argF [H]

Synonym: Sala_1358

Alternate gene names: 103486844

Gene position: 1416173-1415250 (Counterclockwise)

Preceding gene: 103486845

Following gene: 103486843

Centisome position: 42.33

GC content: 67.42

Gene sequence:

>924_bases
GTGCGGCATTTCCTGAACCTCGCCGACGCGGGCGGCGACGCCGTCGCCACGATGATCGCCGACGCGATCGACCGCAAGGC
CGCGCGCGCGGGCTGGCCCAAGGCGCGACCCGACGCCGACGAGCCGCTTGCCGATCATGTGCTGGCGATGGTGTTCGAGA
AGAACTCGACGCGCACCCGCTGCTCGTTCGACATCGCCATCCGCCAACTGGGCGGGGTGCCGATGATCCTCGACGCGGGC
ACGACGCAGCTCGGGCGCGGCGAAACGATCGCCGACACCGCGCGCGTGCTGTCGCGCTACGCCGACGCGATCATGATCCG
CACCGACGATCATGCGAAGGCCGAGGAACTGGCCGCATATGCCAGTGTCCCCGTCATCAACGGTCTCACCGACCTGTCGC
ACCCGTGCCAGATCGTCGCCGACCTGCTCACGATCGTCGAGAGCGGCAAGGCGCTGCCGGGACTGGAGCTTGCATGGCTT
GGCGACGGCAATAATGTGCTGGCCTCGCTGATCGAGGCGGCGGGCCTGTTCGGCTTCAATGTCCGTGTGGGTGTCCCGCA
GGGTTATGAGCCCGACACGGGCTTCATCGAGGCGGCGCGCGCAAGGGGCGCTTGCATCGACATCGTGCGCGATGCGCGCG
AGGCCGTCGCGGGCGCCGACCTGGTCGTCACCGACACCTGGGTGTCGATGGGACAGGACCATGCGCACAACAAGGTTGCG
GCGATGGCGCCGTTTCAGGTCGACGAGCGGCTGATGGCTGCAGCGAAAAGCGATGCGCTCTTCCTCCACTGCCTGCCTGC
GCACCGCGGCGAAGAGGTGGTCGATGCGGTGATCGACGGTGCCCAGTCGCGCGTGTGGGACGAAGCGGAGAACCGCGTCC
ACGCGCAAAAATCGATCCTGCTGTGGACGCTGGCAAAGCTTTGA

Upstream 100 bases:

>100_bases
TTTTCGTTGTTCCGTTCGTGTCGAGCGAAGTCGAGACACCCAATGGCAGCGCGCGAACTCACGGCATCTCGACTTCGCTC
GATGCGAACGGGGAGGTAAT

Downstream 100 bases:

>100_bases
GACGGCAAGCGTGAGCGAAACGATCGAAACCTGGTTCGACCAGCCGCTGGTCTTCACCATCGCTGCGCGCCATGTGCGCG
GGCGACTCGTGCGGCTGGGG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRHFLNLADAGGDAVATMIADAIDRKAARAGWPKARPDADEPLADHVLAMVFEKNSTRTRCSFDIAIRQLGGVPMILDAG
TTQLGRGETIADTARVLSRYADAIMIRTDDHAKAEELAAYASVPVINGLTDLSHPCQIVADLLTIVESGKALPGLELAWL
GDGNNVLASLIEAAGLFGFNVRVGVPQGYEPDTGFIEAARARGACIDIVRDAREAVAGADLVVTDTWVSMGQDHAHNKVA
AMAPFQVDERLMAAAKSDALFLHCLPAHRGEEVVDAVIDGAQSRVWDEAENRVHAQKSILLWTLAKL

Sequences:

>Translated_307_residues
MRHFLNLADAGGDAVATMIADAIDRKAARAGWPKARPDADEPLADHVLAMVFEKNSTRTRCSFDIAIRQLGGVPMILDAG
TTQLGRGETIADTARVLSRYADAIMIRTDDHAKAEELAAYASVPVINGLTDLSHPCQIVADLLTIVESGKALPGLELAWL
GDGNNVLASLIEAAGLFGFNVRVGVPQGYEPDTGFIEAARARGACIDIVRDAREAVAGADLVVTDTWVSMGQDHAHNKVA
AMAPFQVDERLMAAAKSDALFLHCLPAHRGEEVVDAVIDGAQSRVWDEAENRVHAQKSILLWTLAKL
>Mature_307_residues
MRHFLNLADAGGDAVATMIADAIDRKAARAGWPKARPDADEPLADHVLAMVFEKNSTRTRCSFDIAIRQLGGVPMILDAG
TTQLGRGETIADTARVLSRYADAIMIRTDDHAKAEELAAYASVPVINGLTDLSHPCQIVADLLTIVESGKALPGLELAWL
GDGNNVLASLIEAAGLFGFNVRVGVPQGYEPDTGFIEAARARGACIDIVRDAREAVAGADLVVTDTWVSMGQDHAHNKVA
AMAPFQVDERLMAAAKSDALFLHCLPAHRGEEVVDAVIDGAQSRVWDEAENRVHAQKSILLWTLAKL

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=296, Percent_Identity=40.2027027027027, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI18105007, Length=271, Percent_Identity=24.7232472324723, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1790703, Length=334, Percent_Identity=37.7245508982036, Blast_Score=201, Evalue=5e-53,
Organism=Escherichia coli, GI1786469, Length=333, Percent_Identity=36.036036036036, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI48994908, Length=296, Percent_Identity=28.7162162162162, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=27.8846153846154, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193204318, Length=124, Percent_Identity=36.2903225806452, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322373, Length=313, Percent_Identity=33.8658146964856, Blast_Score=179, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6322331, Length=276, Percent_Identity=27.1739130434783, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24642586, Length=270, Percent_Identity=25.9259259259259, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 32835; Mature: 32835

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHFLNLADAGGDAVATMIADAIDRKAARAGWPKARPDADEPLADHVLAMVFEKNSTRTR
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCE
CSFDIAIRQLGGVPMILDAGTTQLGRGETIADTARVLSRYADAIMIRTDDHAKAEELAAY
EHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHH
ASVPVINGLTDLSHPCQIVADLLTIVESGKALPGLELAWLGDGNNVLASLIEAAGLFGFN
HCCCHHCCHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCE
VRVGVPQGYEPDTGFIEAARARGACIDIVRDAREAVAGADLVVTDTWVSMGQDHAHNKVA
EEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCCEE
AMAPFQVDERLMAAAKSDALFLHCLPAHRGEEVVDAVIDGAQSRVWDEAENRVHAQKSIL
EECCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWTLAKL
HHHHHCC
>Mature Secondary Structure
MRHFLNLADAGGDAVATMIADAIDRKAARAGWPKARPDADEPLADHVLAMVFEKNSTRTR
CCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCE
CSFDIAIRQLGGVPMILDAGTTQLGRGETIADTARVLSRYADAIMIRTDDHAKAEELAAY
EHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCEEEEECCCCHHHHHHHHH
ASVPVINGLTDLSHPCQIVADLLTIVESGKALPGLELAWLGDGNNVLASLIEAAGLFGFN
HCCCHHCCHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCE
VRVGVPQGYEPDTGFIEAARARGACIDIVRDAREAVAGADLVVTDTWVSMGQDHAHNKVA
EEECCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCCEE
AMAPFQVDERLMAAAKSDALFLHCLPAHRGEEVVDAVIDGAQSRVWDEAENRVHAQKSIL
EECCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LWTLAKL
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA