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The map label for this gene is bkdB [H]

Identifier: 103486815

GI number: 103486815

Start: 1391318

End: 1392643

Strand: Reverse

Name: bkdB [H]

Synonym: Sala_1329

Alternate gene names: 103486815

Gene position: 1392643-1391318 (Counterclockwise)

Preceding gene: 103486816

Following gene: 103486810

Centisome position: 41.63

GC content: 66.37

Gene sequence:

>1326_bases
ATGGCCCGCTATTCATTCCGTCTGCCCGACATTGGCGAAGGTATCGCCGAGGCCGAGATCGTTGCCTGGCACGTCAAGGT
GGGCGAGCGCGTCGAGGAAGATGCGCAGCTTGCCGACATGATGACCGACAAGGCGACCGTCGAGATGGAATCGCCGGTGT
CGGGCGTCGTGGTCGAACTGGCGGGCGAGGTCGGCGACCTCATTCCAATCGGATCGACGCTGGCGGTGATCGAGACTGAT
GACGATGGTGACGGCGCGCTGGATGCACCGCCCGCCGACACGCCGGTCGAGGACGAGATGGCCGTGGAGACGCCGGGGAC
GGAGGAGGTTTCGGATGCCGAGAAGATCCCCCTCCCGCTTGCGGGAGGGGCTGGGGGAGGGGCTGGGGGAGGGCCTGTCG
AGGCTCCTGCGGTGGTTTCCGAAACAAGCCCTCCCCCGACCCCTCCCGCAAGCGGGAGGGGAAAAAAGGCTGTCCTCGCA
TCCCCCGCCGTCCGCGCGCGTGCGAAGGATCTGGGCATCGACCTGTCGCTGGTCCAATCCGACGGCGACCGCATCCGCCA
CGCCGACCTCGACGCCTATCGGCGCTACAGCGCGGGGCAAGGCTATCACGCCCCCGGCGCGAGCCGCGCGCGCGCCGACG
AGCCGGTCAAGGTCATCGGCATGCGGCGCAGGATCGCCGAGAATATGGCGGCGTCGAAGCGCGCGATCCCACACTTCACC
TATGTCGAGGAAATGGACGTCACCGCGCTGGAAGAGATGCGCGCGGACCTCAATGCCAATCGCGGCAGCCGGCCCAAGCT
GACGATGCTGCCCTTCCTGATCGTCGCGATCTGCCGCACCATTCCTGAGTTTCCGATGATCAACGCGCGCTATGACGACG
AAGCGGGCGTGGTGACGCGATACGGCGCGGTACATCTGGGCATGGCGACGCAGACCGACGCCGGCCTGATGGTCCCCGTT
ATCCGCGACGCGCAGGACAAGAATGTCTGGCAGCTTGCCAGCGAAATCACCCGCCTCGCCGAAGCCGCGCGCACCGGCAA
GGTCAAGGTCGAGGAGTTGACCGGCGGCACGCTGACCGTGACGTCGCTCGGCCCCCTCGGCGGAATCGCGACGACGCCGG
TGATCAACCGCCCCGAGGTCGCGATCATCGGCCCGAACAAGATCGTCGAGCGGCCGATATTCGACGGCGACGACATCCGC
CGCGCCAAGCTCATGAACCTGTCGATCAGCTGCGATCACCGCGTTGTCGATGGCTGGGATGCCGCGAGCTATGTCCAGGC
ATTGAAGAAGCTGATCGAGACGCCGGTGTTGCTGTTCGCGGATTGA

Upstream 100 bases:

>100_bases
GGTTTCGACACGCCCTATCCGCACAGCCTGGAATGGGCCTATTTCCCCGGCCCGGTGCGCATTGCGACCGCGCTGACCAA
GATTTTGAAGGACTGACGCC

Downstream 100 bases:

>100_bases
GCTTCCCTCGTCATTGCGAGGAGCGAAGTGACGAAGCAATCTCCAGCGGGTGGACTTATGGGTCGTTAGCTGGAGATTGC
TTCGCTTCGCTCGCAATGAC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MARYSFRLPDIGEGIAEAEIVAWHVKVGERVEEDAQLADMMTDKATVEMESPVSGVVVELAGEVGDLIPIGSTLAVIETD
DDGDGALDAPPADTPVEDEMAVETPGTEEVSDAEKIPLPLAGGAGGGAGGGPVEAPAVVSETSPPPTPPASGRGKKAVLA
SPAVRARAKDLGIDLSLVQSDGDRIRHADLDAYRRYSAGQGYHAPGASRARADEPVKVIGMRRRIAENMAASKRAIPHFT
YVEEMDVTALEEMRADLNANRGSRPKLTMLPFLIVAICRTIPEFPMINARYDDEAGVVTRYGAVHLGMATQTDAGLMVPV
IRDAQDKNVWQLASEITRLAEAARTGKVKVEELTGGTLTVTSLGPLGGIATTPVINRPEVAIIGPNKIVERPIFDGDDIR
RAKLMNLSISCDHRVVDGWDAASYVQALKKLIETPVLLFAD

Sequences:

>Translated_441_residues
MARYSFRLPDIGEGIAEAEIVAWHVKVGERVEEDAQLADMMTDKATVEMESPVSGVVVELAGEVGDLIPIGSTLAVIETD
DDGDGALDAPPADTPVEDEMAVETPGTEEVSDAEKIPLPLAGGAGGGAGGGPVEAPAVVSETSPPPTPPASGRGKKAVLA
SPAVRARAKDLGIDLSLVQSDGDRIRHADLDAYRRYSAGQGYHAPGASRARADEPVKVIGMRRRIAENMAASKRAIPHFT
YVEEMDVTALEEMRADLNANRGSRPKLTMLPFLIVAICRTIPEFPMINARYDDEAGVVTRYGAVHLGMATQTDAGLMVPV
IRDAQDKNVWQLASEITRLAEAARTGKVKVEELTGGTLTVTSLGPLGGIATTPVINRPEVAIIGPNKIVERPIFDGDDIR
RAKLMNLSISCDHRVVDGWDAASYVQALKKLIETPVLLFAD
>Mature_440_residues
ARYSFRLPDIGEGIAEAEIVAWHVKVGERVEEDAQLADMMTDKATVEMESPVSGVVVELAGEVGDLIPIGSTLAVIETDD
DGDGALDAPPADTPVEDEMAVETPGTEEVSDAEKIPLPLAGGAGGGAGGGPVEAPAVVSETSPPPTPPASGRGKKAVLAS
PAVRARAKDLGIDLSLVQSDGDRIRHADLDAYRRYSAGQGYHAPGASRARADEPVKVIGMRRRIAENMAASKRAIPHFTY
VEEMDVTALEEMRADLNANRGSRPKLTMLPFLIVAICRTIPEFPMINARYDDEAGVVTRYGAVHLGMATQTDAGLMVPVI
RDAQDKNVWQLASEITRLAEAARTGKVKVEELTGGTLTVTSLGPLGGIATTPVINRPEVAIIGPNKIVERPIFDGDDIRR
AKLMNLSISCDHRVVDGWDAASYVQALKKLIETPVLLFAD

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=466, Percent_Identity=28.3261802575107, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI31711992, Length=451, Percent_Identity=28.6031042128603, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI19923748, Length=240, Percent_Identity=30.8333333333333, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI203098816, Length=225, Percent_Identity=29.3333333333333, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI203098753, Length=226, Percent_Identity=29.2035398230088, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI260898739, Length=169, Percent_Identity=29.585798816568, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786946, Length=452, Percent_Identity=26.9911504424779, Blast_Score=141, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI17537937, Length=456, Percent_Identity=29.1666666666667, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17560088, Length=436, Percent_Identity=28.8990825688073, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=452, Percent_Identity=27.212389380531, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=232, Percent_Identity=24.1379310344828, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320352, Length=398, Percent_Identity=29.1457286432161, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324258, Length=462, Percent_Identity=24.4588744588745, Blast_Score=120, Evalue=6e-28,
Organism=Drosophila melanogaster, GI18859875, Length=460, Percent_Identity=28.2608695652174, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=30.7017543859649, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24645909, Length=232, Percent_Identity=29.3103448275862, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=30.7017543859649, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 46835; Mature: 46704

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYSFRLPDIGEGIAEAEIVAWHVKVGERVEEDAQLADMMTDKATVEMESPVSGVVVEL
CCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHEE
AGEVGDLIPIGSTLAVIETDDDGDGALDAPPADTPVEDEMAVETPGTEEVSDAEKIPLPL
HHHCCCEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHCCCEE
AGGAGGGAGGGPVEAPAVVSETSPPPTPPASGRGKKAVLASPAVRARAKDLGIDLSLVQS
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCEEEEEEC
DGDRIRHADLDAYRRYSAGQGYHAPGASRARADEPVKVIGMRRRIAENMAASKRAIPHFT
CCCEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
YVEEMDVTALEEMRADLNANRGSRPKLTMLPFLIVAICRTIPEFPMINARYDDEAGVVTR
HHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEE
YGAVHLGMATQTDAGLMVPVIRDAQDKNVWQLASEITRLAEAARTGKVKVEELTGGTLTV
CCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEE
TSLGPLGGIATTPVINRPEVAIIGPNKIVERPIFDGDDIRRAKLMNLSISCDHRVVDGWD
EECCCCCCCCCCCCCCCCCEEEECCHHHHHCCCCCCCCCCEEEEEEEEEECCCEEECCCC
AASYVQALKKLIETPVLLFAD
HHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
ARYSFRLPDIGEGIAEAEIVAWHVKVGERVEEDAQLADMMTDKATVEMESPVSGVVVEL
CCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHEE
AGEVGDLIPIGSTLAVIETDDDGDGALDAPPADTPVEDEMAVETPGTEEVSDAEKIPLPL
HHHCCCEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHCCCEE
AGGAGGGAGGGPVEAPAVVSETSPPPTPPASGRGKKAVLASPAVRARAKDLGIDLSLVQS
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECCHHHHHHHHCCCEEEEEEC
DGDRIRHADLDAYRRYSAGQGYHAPGASRARADEPVKVIGMRRRIAENMAASKRAIPHFT
CCCEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEE
YVEEMDVTALEEMRADLNANRGSRPKLTMLPFLIVAICRTIPEFPMINARYDDEAGVVTR
HHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCEEEE
YGAVHLGMATQTDAGLMVPVIRDAQDKNVWQLASEITRLAEAARTGKVKVEELTGGTLTV
CCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEE
TSLGPLGGIATTPVINRPEVAIIGPNKIVERPIFDGDDIRRAKLMNLSISCDHRVVDGWD
EECCCCCCCCCCCCCCCCCEEEECCHHHHHCCCCCCCCCCEEEEEEEEEECCCEEECCCC
AASYVQALKKLIETPVLLFAD
HHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 3046941 [H]