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The map label for this gene is lipA

Identifier: 103486767

GI number: 103486767

Start: 1338188

End: 1339123

Strand: Reverse

Name: lipA

Synonym: Sala_1281

Alternate gene names: 103486767

Gene position: 1339123-1338188 (Counterclockwise)

Preceding gene: 103486769

Following gene: 103486766

Centisome position: 40.03

GC content: 65.49

Gene sequence:

>936_bases
ATGAACGCTCCTGCATCCCCCGCCGCCTCGCCCAGCCAGCGCGCGCGCAAGCCCGACTGGATCCGCGTCAAGGCACCGAC
CAGCCCCGGCTATGCCGAAACGCGCAAGCTGATGCGCGAGCTGAACCTGCACACCGTGTGCGAGGAAGCGGCGTGCCCGA
ACATCGGCGAGTGCTGGACCAAGAAGCACGCGACGGTGATGATCCTTGGCGACACCTGCACGCGCGCCTGCGCTTTCTGC
AACGTCAAGACCGGCATGCCGCGCCCCGTCGACCTGCTCGAACCGGAACATACGGCGATCGCTGCGGCGAAGATGGGACT
CAGCCACATCGTCATCACCTCGGTCGACCGCGACGACCTGCCCGACGGCGGCGCGAGTCAGTTCGTCAAGGTCATCAACG
CGCTGCGCCGCGAAACGCCGCAGACGACGATCGAGATACTGACCCCGGACTTTCGCAACAAACCCGAAAGCGCGGTCGCC
GCGATCGTCGATGCGCGCCCCGACGTCTATAACCACAACCTCGAAACCGTGCCGCGGCTCTATCCGACGATCCGTCCCGG
CGCGCGCTATTATGCGTCGCTCCGCCTGCTCGAAAGCGTCAAGCGGCGCGACCCGTCGATCTTCACCAAGTCAGGCATCA
TGCTCGGGCTCGGTGAGGAGCGGATGGAGGTGCATCAGGTGATGGACGACATGCGCAGCGCCGACATCGATTTCATGACG
ATGGGCCAATATCTGCAACCGACGCCGAAACACGCCAAGGTGATCGACTTCGTCACCCCGCAGGCGTTCGACGCCTATGC
GCAGATCGCGCGCGCCAAGGGTTTCCTGCAGGTGGCGTCATCGCCGCTGACGCGCTCCAGCTATCATGCGGGCGACGATT
TCGAACATATGCGCGCGGCGCGCGAGGCGCAGCTCGCGCGCGTCCGCGCCGACTGA

Upstream 100 bases:

>100_bases
CGGCGATAGCCCTCGATCATGTCGGCAAAGTGAGTCATGGCGGCCCCATAGCATAGGTAGAGAGGTTGAGAAGAGGGGCA
TAGGCGACTATGTGGGCGCG

Downstream 100 bases:

>100_bases
GCGTCGCTTGCCAAGGCACCACGAAACGCGCGATCTGCCGTACAGCGCCGAACAGATGTTCGCGCTGGTGACCGATATCG
CACGCTATCCCGAGTTCCTG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MNAPASPAASPSQRARKPDWIRVKAPTSPGYAETRKLMRELNLHTVCEEAACPNIGECWTKKHATVMILGDTCTRACAFC
NVKTGMPRPVDLLEPEHTAIAAAKMGLSHIVITSVDRDDLPDGGASQFVKVINALRRETPQTTIEILTPDFRNKPESAVA
AIVDARPDVYNHNLETVPRLYPTIRPGARYYASLRLLESVKRRDPSIFTKSGIMLGLGEERMEVHQVMDDMRSADIDFMT
MGQYLQPTPKHAKVIDFVTPQAFDAYAQIARAKGFLQVASSPLTRSSYHAGDDFEHMRAAREAQLARVRAD

Sequences:

>Translated_311_residues
MNAPASPAASPSQRARKPDWIRVKAPTSPGYAETRKLMRELNLHTVCEEAACPNIGECWTKKHATVMILGDTCTRACAFC
NVKTGMPRPVDLLEPEHTAIAAAKMGLSHIVITSVDRDDLPDGGASQFVKVINALRRETPQTTIEILTPDFRNKPESAVA
AIVDARPDVYNHNLETVPRLYPTIRPGARYYASLRLLESVKRRDPSIFTKSGIMLGLGEERMEVHQVMDDMRSADIDFMT
MGQYLQPTPKHAKVIDFVTPQAFDAYAQIARAKGFLQVASSPLTRSSYHAGDDFEHMRAAREAQLARVRAD
>Mature_311_residues
MNAPASPAASPSQRARKPDWIRVKAPTSPGYAETRKLMRELNLHTVCEEAACPNIGECWTKKHATVMILGDTCTRACAFC
NVKTGMPRPVDLLEPEHTAIAAAKMGLSHIVITSVDRDDLPDGGASQFVKVINALRRETPQTTIEILTPDFRNKPESAVA
AIVDARPDVYNHNLETVPRLYPTIRPGARYYASLRLLESVKRRDPSIFTKSGIMLGLGEERMEVHQVMDDMRSADIDFMT
MGQYLQPTPKHAKVIDFVTPQAFDAYAQIARAKGFLQVASSPLTRSSYHAGDDFEHMRAAREAQLARVRAD

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=301, Percent_Identity=45.1827242524917, Blast_Score=279, Evalue=2e-75,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=47.2, Blast_Score=243, Evalue=1e-64,
Organism=Escherichia coli, GI1786846, Length=276, Percent_Identity=48.9130434782609, Blast_Score=270, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI32564533, Length=257, Percent_Identity=48.6381322957198, Blast_Score=256, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6324770, Length=299, Percent_Identity=45.1505016722408, Blast_Score=264, Evalue=1e-71,
Organism=Drosophila melanogaster, GI221513272, Length=289, Percent_Identity=48.0968858131488, Blast_Score=282, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_SPHAL (Q1GTM7)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616328.1
- ProteinModelPortal:   Q1GTM7
- SMR:   Q1GTM7
- GeneID:   4082532
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1281
- NMPDR:   fig|317655.9.peg.1216
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   SALA317655:SALA_1281-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 34501; Mature: 34501

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPASPAASPSQRARKPDWIRVKAPTSPGYAETRKLMRELNLHTVCEEAACPNIGECWT
CCCCCCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
KKHATVMILGDTCTRACAFCNVKTGMPRPVDLLEPEHTAIAAAKMGLSHIVITSVDRDDL
CCCCEEEEECCHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHCCCEEEEEECCCCCC
PDGGASQFVKVINALRRETPQTTIEILTPDFRNKPESAVAAIVDARPDVYNHNLETVPRL
CCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHCCCHHHHHHH
YPTIRPGARYYASLRLLESVKRRDPSIFTKSGIMLGLGEERMEVHQVMDDMRSADIDFMT
CCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCEEEECCHHHHHHHHHHHHHHHCCCCCEE
MGQYLQPTPKHAKVIDFVTPQAFDAYAQIARAKGFLQVASSPLTRSSYHAGDDFEHMRAA
CCHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
REAQLARVRAD
HHHHHHHHCCC
>Mature Secondary Structure
MNAPASPAASPSQRARKPDWIRVKAPTSPGYAETRKLMRELNLHTVCEEAACPNIGECWT
CCCCCCCCCCCHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHC
KKHATVMILGDTCTRACAFCNVKTGMPRPVDLLEPEHTAIAAAKMGLSHIVITSVDRDDL
CCCCEEEEECCHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHCCCEEEEEECCCCCC
PDGGASQFVKVINALRRETPQTTIEILTPDFRNKPESAVAAIVDARPDVYNHNLETVPRL
CCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCHHCCCHHHHHHH
YPTIRPGARYYASLRLLESVKRRDPSIFTKSGIMLGLGEERMEVHQVMDDMRSADIDFMT
CCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCEEEECCHHHHHHHHHHHHHHHCCCCCEE
MGQYLQPTPKHAKVIDFVTPQAFDAYAQIARAKGFLQVASSPLTRSSYHAGDDFEHMRAA
CCHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
REAQLARVRAD
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA