Click here to switch to the map view.

The map label for this gene is ispDF

Identifier: 103486764

GI number: 103486764

Start: 1336018

End: 1337196

Strand: Direct

Name: ispDF

Synonym: Sala_1278

Alternate gene names: 103486764

Gene position: 1336018-1337196 (Clockwise)

Preceding gene: 103486756

Following gene: 103486765

Centisome position: 39.94

GC content: 68.87

Gene sequence:

>1179_bases
ATGACCGAGTCCGCTGCCCCGTCTCCGCGCTGCGTCGCCGCGATCCTGCTCGCCGGCGGCCGCGGGCTGCGTGCTGGCTT
TGGACGGCCCAAGCAGCTCGAGTGGCTTTGTGGCAAACCGGTGCTGCGCTGGAGCCTCGACGTTCTGGCGGGCTACCCCG
GCCTTTCGGGCGGCGTGCTGGTGGCGAACCATGACGTCATGGCGGCGACATACCCCTTGCCGGAAGGGTGGATGGCCGCC
GATCCCGGCGTTGAGCGACAACAATCGGTTGCCAATGCGCTGTCCGCGCTGACCGACTGGCCCGACGATGCAGTCGTGCT
CGTCCATGATGCCGCCCGCCCCGGCGTCGATTCCGCCGTCGTCGATCGGCTGCTGGCCGCACTCGACGGCGCCGACGCCG
CGATCCCGGTGCTGCCCGTCCCCGACACGCTGGTCGAGGCCGCCGATGGCGCGGCGGGCGCGGTCGTCGCGCGCGACCGG
CTGGCGCGCGTGCAGACGCCGCAGGCCTTTCGCCTCGGCACCCTGCGCCGCGCGCATGCCGAAGCATCCGGCGCCGCTGC
GACCGACGATGCGCAGCTCGTCCGCAGCCTTGGCGTCTCGGTGGCGACGGTCGAAGGTGATGCGCGGCTCCACAAATTGA
CATATGCCGCGGATATGGCGATTCTGGCCGGGCTGCTGGGGACGAAGGACATGATGCGAACCGCCGTGGGCATGGGCTAT
GACGTCCACCGCCTCGTCGAGGGCAAGCCCTTGTGGATCGGCGGTATAGAAATAGCGCATAGTCATGGACTTGAAGGGCA
TAGCGATGCCGACGTCGGGCTTCACGCGCTGACCGATGCGATCCTTGGCGCGCTGGGCGACGGCGACATTGGCGATCATT
TCCCACCGAGCGACCCGCAATGGCGCGGCGCCGCATCGCACCATTTCCTCTCCTTTGCGGGCGAGCGCGTCGCCGCAAAT
GGCGGACGGATCGGCCATCTCGACCTCACCATCATCGCGGAAGCGCCGCGGATCGGCCCGCACCGCGTCGCCATCCGCGA
CCGCATTGCCGAAATCCTTGCGATTCCGGCGGGAAGGGTTAGTGTGAAGGCGACAACGACGGAGCGGCTGGGTTTCACCG
GCCGCCGCGAGGGGATCGCGGCCCAGGCCGTTGCAACCATCCAACTTCCGGAGACCTGA

Upstream 100 bases:

>100_bases
CCATGGCGGGGTGCTTTCGTCAGACTGCCTAAATTTTGAGCAGCCCCTACACGCTAAGCCGCTTTCCCGCAAGGCGTGGC
TGCGCTAACCGGCGCGCACC

Downstream 100 bases:

>100_bases
AGCCGTGCAGCCCGACGCCGAATCGCGCCTCATCGCGCGTGATGACCTCGCCGCGAGCGTGCTCGCCCAGAATCGCGCGG
CCGGGCGCAAGATCGCGGTC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MTESAAPSPRCVAAILLAGGRGLRAGFGRPKQLEWLCGKPVLRWSLDVLAGYPGLSGGVLVANHDVMAATYPLPEGWMAA
DPGVERQQSVANALSALTDWPDDAVVLVHDAARPGVDSAVVDRLLAALDGADAAIPVLPVPDTLVEAADGAAGAVVARDR
LARVQTPQAFRLGTLRRAHAEASGAAATDDAQLVRSLGVSVATVEGDARLHKLTYAADMAILAGLLGTKDMMRTAVGMGY
DVHRLVEGKPLWIGGIEIAHSHGLEGHSDADVGLHALTDAILGALGDGDIGDHFPPSDPQWRGAASHHFLSFAGERVAAN
GGRIGHLDLTIIAEAPRIGPHRVAIRDRIAEILAIPAGRVSVKATTTERLGFTGRREGIAAQAVATIQLPET

Sequences:

>Translated_392_residues
MTESAAPSPRCVAAILLAGGRGLRAGFGRPKQLEWLCGKPVLRWSLDVLAGYPGLSGGVLVANHDVMAATYPLPEGWMAA
DPGVERQQSVANALSALTDWPDDAVVLVHDAARPGVDSAVVDRLLAALDGADAAIPVLPVPDTLVEAADGAAGAVVARDR
LARVQTPQAFRLGTLRRAHAEASGAAATDDAQLVRSLGVSVATVEGDARLHKLTYAADMAILAGLLGTKDMMRTAVGMGY
DVHRLVEGKPLWIGGIEIAHSHGLEGHSDADVGLHALTDAILGALGDGDIGDHFPPSDPQWRGAASHHFLSFAGERVAAN
GGRIGHLDLTIIAEAPRIGPHRVAIRDRIAEILAIPAGRVSVKATTTERLGFTGRREGIAAQAVATIQLPET
>Mature_391_residues
TESAAPSPRCVAAILLAGGRGLRAGFGRPKQLEWLCGKPVLRWSLDVLAGYPGLSGGVLVANHDVMAATYPLPEGWMAAD
PGVERQQSVANALSALTDWPDDAVVLVHDAARPGVDSAVVDRLLAALDGADAAIPVLPVPDTLVEAADGAAGAVVARDRL
ARVQTPQAFRLGTLRRAHAEASGAAATDDAQLVRSLGVSVATVEGDARLHKLTYAADMAILAGLLGTKDMMRTAVGMGYD
VHRLVEGKPLWIGGIEIAHSHGLEGHSDADVGLHALTDAILGALGDGDIGDHFPPSDPQWRGAASHHFLSFAGERVAANG
GRIGHLDLTIIAEAPRIGPHRVAIRDRIAEILAIPAGRVSVKATTTERLGFTGRREGIAAQAVATIQLPET

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family

Homologues:

Organism=Homo sapiens, GI157412259, Length=246, Percent_Identity=26.8292682926829, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789103, Length=152, Percent_Identity=51.9736842105263, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPDF_SPHAL (Q1GTN0)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616325.1
- ProteinModelPortal:   Q1GTN0
- SMR:   Q1GTN0
- GeneID:   4082529
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1278
- NMPDR:   fig|317655.9.peg.1214
- HOGENOM:   HBG672839
- OMA:   IVLIHDA
- BioCyc:   SALA317655:SALA_1278-MONOMER
- BRENDA:   2.7.7.60
- BRENDA:   4.6.1.12
- HAMAP:   MF_01520
- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00453
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF01128 IspD; PF02542 YgbB; SSF69765 YgbB_synth

EC number: =2.7.7.60; =4.6.1.12

Molecular weight: Translated: 40698; Mature: 40567

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS01295 ISPD; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTESAAPSPRCVAAILLAGGRGLRAGFGRPKQLEWLCGKPVLRWSLDVLAGYPGLSGGVL
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCEE
VANHDVMAATYPLPEGWMAADPGVERQQSVANALSALTDWPDDAVVLVHDAARPGVDSAV
EECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHH
VDRLLAALDGADAAIPVLPVPDTLVEAADGAAGAVVARDRLARVQTPQAFRLGTLRRAHA
HHHHHHHHCCCCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
EASGAAATDDAQLVRSLGVSVATVEGDARLHKLTYAADMAILAGLLGTKDMMRTAVGMGY
CCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
DVHRLVEGKPLWIGGIEIAHSHGLEGHSDADVGLHALTDAILGALGDGDIGDHFPPSDPQ
CHHHHHCCCCEEEECEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
WRGAASHHFLSFAGERVAANGGRIGHLDLTIIAEAPRIGPHRVAIRDRIAEILAIPAGRV
CCCCHHHHHHHHCCCEEECCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCE
SVKATTTERLGFTGRREGIAAQAVATIQLPET
EEEEECHHHCCCCCCCCCCEEEEEEEEECCCC
>Mature Secondary Structure 
TESAAPSPRCVAAILLAGGRGLRAGFGRPKQLEWLCGKPVLRWSLDVLAGYPGLSGGVL
CCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCCCEE
VANHDVMAATYPLPEGWMAADPGVERQQSVANALSALTDWPDDAVVLVHDAARPGVDSAV
EECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHH
VDRLLAALDGADAAIPVLPVPDTLVEAADGAAGAVVARDRLARVQTPQAFRLGTLRRAHA
HHHHHHHHCCCCCCEEEECCCHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
EASGAAATDDAQLVRSLGVSVATVEGDARLHKLTYAADMAILAGLLGTKDMMRTAVGMGY
CCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
DVHRLVEGKPLWIGGIEIAHSHGLEGHSDADVGLHALTDAILGALGDGDIGDHFPPSDPQ
CHHHHHCCCCEEEECEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
WRGAASHHFLSFAGERVAANGGRIGHLDLTIIAEAPRIGPHRVAIRDRIAEILAIPAGRV
CCCCHHHHHHHHCCCEEECCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCE
SVKATTTERLGFTGRREGIAAQAVATIQLPET
EEEEECHHHCCCCCCCCCCEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA