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The map label for this gene is alkK [H]

Identifier: 103486668

GI number: 103486668

Start: 1218331

End: 1219935

Strand: Direct

Name: alkK [H]

Synonym: Sala_1180

Alternate gene names: 103486668

Gene position: 1218331-1219935 (Clockwise)

Preceding gene: 103486667

Following gene: 103486673

Centisome position: 36.42

GC content: 63.36

Gene sequence:

>1605_bases
ATGGGAATGCAGGGCCAGCCGCTGCTCGTCACCAGCCTGATCGACCACGCCGCACGCGAACATGCGGGACGCGAGATTGT
GTCGCGATGGGCCGACGGGAGGGTGACGCGATCGAACTGGGGCGAAGTCGGGACCGACGCCCGGCGCTTTGCCGCCGCGA
TGGTGAAGCTGGGCATGAAAAAGGGCGACCGTATCGCGACGCTGGCGATGAACCACGGTCACCATCTGGTGAGCTGGTAC
GGCACCGCGGGCATGGGCGGGGTGCTGCACACGGTGAACCCGCGGCTGTTCGACGAACAGCTTGTCTACATCATCAATCA
TGCCGAAGACCGGGTGCTGTTCTTCGACGCGGCCTTCCTGCCGATCGTCGAGCGGCTGCGTGGCCAGCTGACCAGTGTCG
AGCATTTCGTCCTGTTCGATGCGTCGGCGCAGGGCGATTACAAATCCTATCGCGACCTGGTCGATGCCGAGGATGGCGAT
TTCGAGTGGGTCGAACTGGGCGAACGCGATCCGGTCGGGCTTTGCTACACCAGCGGCACGACGGGCAACCCCAAGGGCGT
GCTTTACGAGCATCGCTCGAACGTCATCCATGCAATCACCGAAATCCAGCCGGACGCCTTTGACCTGTCGAGCCGGAGCG
TCATCCTGCCGATCGTGCCGATGTTCCACGCGAACAGTTGGGGCATTCCCTTTGCAGCGGCGACGGTGGGCGCGAAGCTG
GTCTTTTCGGCGACCAATGATGCGCAGACCCTGTGTGACCTGATGCACGCCGAGGGCGTCACCCACAGCGCCGGGGTGCC
GACCGTCTGGCTGGCGATGTTCGCGCATATGGATGCGACGGGCATGGGTTATGGCGCGCTCAAGCATGTCATCATCGGCG
GGTCGGCGGCCCCGCGCGCGATGATTGAACGCTTTATGAAGGCCGGTGTCTATGTCGGGCACGCCTGGGGCATGACCGAG
ACCAGCCCGATCGGGACGATGGGCAAGCGGCCGTGGAACTGGGATGCGCTGAGTTTTGACGAGCAGGTCGACATCATCTG
CCGCCAGGGCTGTCCGCCGTTCGGGGTCGAACTGCGGATCGTCGATGACGAGGGCAAGGAACTGCCGCGCGACGGCCAGA
CCAGCGGACGGCTCCAGATCCGTGGGCCGTGGATCATTCAGCGTTATTTCAAGGCCGATGCCGACGCTGCCGACGCCGAT
GGCTGGTTCGACACGGGCGATGTGTCGGTGCTCCATCCCGATGGCGTCATGCAGATCACCGACCGCGCGAAGGACGTCAT
CAAGTCGGGCGGCGAATGGATCAGCTCGATCGAGCTGGAAAATGCCGCGGTCGGAGCGCCGGGCGTGCAGGAAGCCGCTG
CGGTCGGCGTCTATCACCCCAAATGGGACGAGCGGCCGATCCTGCTGATCGTGAAGAAACCGGGTGCCGAGGTGAGCGAG
GCGGCGATCATTGAATATCTGAAGGACAAGGTCGCCAAATGGTGGCTACCCGACGAGGTCGTGTTCGTCGACGAACTGCC
GCACACCGCCACGGGCAAGATTTTGAAGCGCCAGATCCGCGATGACTATAAGGATTACAAGCTGAAGTCGCTGGCAGAGG
CGTAA

Upstream 100 bases:

>100_bases
GCGCGAGTTCCGGGTGCGCTGACGCGCTTTGCCCCTTGCACGGCGGCATGGTCGCACCCTATTCCTGAGCGGCAACGACA
AGAATAGAGGATGCCGCACC

Downstream 100 bases:

>100_bases
GTGAAAAGCGTCGCCCCCGCCTTCGCGGAGGCGACGGTATTGGTTCAGAACAGCTCCATCTGCCCATCGCGCTCGGGCGG
CCTGAACAGGTCCGAGCGGA

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MGMQGQPLLVTSLIDHAAREHAGREIVSRWADGRVTRSNWGEVGTDARRFAAAMVKLGMKKGDRIATLAMNHGHHLVSWY
GTAGMGGVLHTVNPRLFDEQLVYIINHAEDRVLFFDAAFLPIVERLRGQLTSVEHFVLFDASAQGDYKSYRDLVDAEDGD
FEWVELGERDPVGLCYTSGTTGNPKGVLYEHRSNVIHAITEIQPDAFDLSSRSVILPIVPMFHANSWGIPFAAATVGAKL
VFSATNDAQTLCDLMHAEGVTHSAGVPTVWLAMFAHMDATGMGYGALKHVIIGGSAAPRAMIERFMKAGVYVGHAWGMTE
TSPIGTMGKRPWNWDALSFDEQVDIICRQGCPPFGVELRIVDDEGKELPRDGQTSGRLQIRGPWIIQRYFKADADAADAD
GWFDTGDVSVLHPDGVMQITDRAKDVIKSGGEWISSIELENAAVGAPGVQEAAAVGVYHPKWDERPILLIVKKPGAEVSE
AAIIEYLKDKVAKWWLPDEVVFVDELPHTATGKILKRQIRDDYKDYKLKSLAEA

Sequences:

>Translated_534_residues
MGMQGQPLLVTSLIDHAAREHAGREIVSRWADGRVTRSNWGEVGTDARRFAAAMVKLGMKKGDRIATLAMNHGHHLVSWY
GTAGMGGVLHTVNPRLFDEQLVYIINHAEDRVLFFDAAFLPIVERLRGQLTSVEHFVLFDASAQGDYKSYRDLVDAEDGD
FEWVELGERDPVGLCYTSGTTGNPKGVLYEHRSNVIHAITEIQPDAFDLSSRSVILPIVPMFHANSWGIPFAAATVGAKL
VFSATNDAQTLCDLMHAEGVTHSAGVPTVWLAMFAHMDATGMGYGALKHVIIGGSAAPRAMIERFMKAGVYVGHAWGMTE
TSPIGTMGKRPWNWDALSFDEQVDIICRQGCPPFGVELRIVDDEGKELPRDGQTSGRLQIRGPWIIQRYFKADADAADAD
GWFDTGDVSVLHPDGVMQITDRAKDVIKSGGEWISSIELENAAVGAPGVQEAAAVGVYHPKWDERPILLIVKKPGAEVSE
AAIIEYLKDKVAKWWLPDEVVFVDELPHTATGKILKRQIRDDYKDYKLKSLAEA
>Mature_533_residues
GMQGQPLLVTSLIDHAAREHAGREIVSRWADGRVTRSNWGEVGTDARRFAAAMVKLGMKKGDRIATLAMNHGHHLVSWYG
TAGMGGVLHTVNPRLFDEQLVYIINHAEDRVLFFDAAFLPIVERLRGQLTSVEHFVLFDASAQGDYKSYRDLVDAEDGDF
EWVELGERDPVGLCYTSGTTGNPKGVLYEHRSNVIHAITEIQPDAFDLSSRSVILPIVPMFHANSWGIPFAAATVGAKLV
FSATNDAQTLCDLMHAEGVTHSAGVPTVWLAMFAHMDATGMGYGALKHVIIGGSAAPRAMIERFMKAGVYVGHAWGMTET
SPIGTMGKRPWNWDALSFDEQVDIICRQGCPPFGVELRIVDDEGKELPRDGQTSGRLQIRGPWIIQRYFKADADAADADG
WFDTGDVSVLHPDGVMQITDRAKDVIKSGGEWISSIELENAAVGAPGVQEAAAVGVYHPKWDERPILLIVKKPGAEVSEA
AIIEYLKDKVAKWWLPDEVVFVDELPHTATGKILKRQIRDDYKDYKLKSLAEA

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=533, Percent_Identity=26.6416510318949, Blast_Score=142, Evalue=8e-34,
Organism=Homo sapiens, GI187761345, Length=533, Percent_Identity=24.765478424015, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI187761343, Length=533, Percent_Identity=24.765478424015, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI38505220, Length=531, Percent_Identity=23.3521657250471, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI122937307, Length=522, Percent_Identity=24.5210727969349, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI115511026, Length=526, Percent_Identity=22.6235741444867, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI42544132, Length=508, Percent_Identity=22.4409448818898, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI157311624, Length=551, Percent_Identity=21.7785843920145, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI157311622, Length=551, Percent_Identity=21.7785843920145, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI28416953, Length=532, Percent_Identity=22.7443609022556, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI58082049, Length=555, Percent_Identity=22.5225225225225, Blast_Score=87, Evalue=5e-17,
Organism=Escherichia coli, GI1788107, Length=537, Percent_Identity=27.56052141527, Blast_Score=168, Evalue=8e-43,
Organism=Escherichia coli, GI221142682, Length=523, Percent_Identity=25.8126195028681, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI145693145, Length=484, Percent_Identity=26.2396694214876, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1786810, Length=359, Percent_Identity=30.3621169916435, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1789201, Length=550, Percent_Identity=22.1818181818182, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788595, Length=511, Percent_Identity=22.1135029354207, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1790505, Length=180, Percent_Identity=31.1111111111111, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71994703, Length=527, Percent_Identity=27.3244781783681, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI71994694, Length=527, Percent_Identity=27.3244781783681, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI71994690, Length=527, Percent_Identity=27.3244781783681, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17538037, Length=533, Percent_Identity=24.015009380863, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17558820, Length=507, Percent_Identity=24.8520710059172, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17560308, Length=534, Percent_Identity=26.7790262172285, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32563687, Length=499, Percent_Identity=25.4509018036072, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=553, Percent_Identity=23.3273056057866, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17559526, Length=517, Percent_Identity=23.4042553191489, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17531443, Length=510, Percent_Identity=22.3529411764706, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI71985884, Length=392, Percent_Identity=26.7857142857143, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17560140, Length=507, Percent_Identity=21.8934911242604, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71996755, Length=494, Percent_Identity=23.0769230769231, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=394, Percent_Identity=26.9035532994924, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320852, Length=452, Percent_Identity=23.6725663716814, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI18859661, Length=499, Percent_Identity=27.0541082164329, Blast_Score=135, Evalue=9e-32,
Organism=Drosophila melanogaster, GI21355181, Length=501, Percent_Identity=23.9520958083832, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI21356947, Length=508, Percent_Identity=23.6220472440945, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24581924, Length=532, Percent_Identity=26.1278195488722, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24653035, Length=496, Percent_Identity=22.9838709677419, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648260, Length=514, Percent_Identity=21.7898832684825, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI161076582, Length=507, Percent_Identity=22.2879684418146, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922652, Length=519, Percent_Identity=22.1579961464355, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI21358303, Length=472, Percent_Identity=20.3389830508475, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281365686, Length=501, Percent_Identity=20.3592814371257, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI20130357, Length=368, Percent_Identity=24.7282608695652, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24648257, Length=512, Percent_Identity=21.6796875, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356441, Length=520, Percent_Identity=21.1538461538462, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 58728; Mature: 58597

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMQGQPLLVTSLIDHAAREHAGREIVSRWADGRVTRSNWGEVGTDARRFAAAMVKLGMK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHCC
KGDRIATLAMNHGHHLVSWYGTAGMGGVLHTVNPRLFDEQLVYIINHAEDRVLFFDAAFL
CCCEEEEEEECCCCEEEEEECCCCCCCEEECCCCCHHCCEEEEEEECCCCEEEEEEHHHH
PIVERLRGQLTSVEHFVLFDASAQGDYKSYRDLVDAEDGDFEWVELGERDPVGLCYTSGT
HHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCC
TGNPKGVLYEHRSNVIHAITEIQPDAFDLSSRSVILPIVPMFHANSWGIPFAAATVGAKL
CCCCCCEEECCCCCCEEHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHCCEE
VFSATNDAQTLCDLMHAEGVTHSAGVPTVWLAMFAHMDATGMGYGALKHVIIGGSAAPRA
EEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHEEECCCCCHHH
MIERFMKAGVYVGHAWGMTETSPIGTMGKRPWNWDALSFDEQVDIICRQGCPPFGVELRI
HHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEE
VDDEGKELPRDGQTSGRLQIRGPWIIQRYFKADADAADADGWFDTGDVSVLHPDGVMQIT
ECCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHH
DRAKDVIKSGGEWISSIELENAAVGAPGVQEAAAVGVYHPKWDERPILLIVKKPGAEVSE
HHHHHHHHCCCHHHHHEEECCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHH
AAIIEYLKDKVAKWWLPDEVVFVDELPHTATGKILKRQIRDDYKDYKLKSLAEA
HHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GMQGQPLLVTSLIDHAAREHAGREIVSRWADGRVTRSNWGEVGTDARRFAAAMVKLGMK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHHCC
KGDRIATLAMNHGHHLVSWYGTAGMGGVLHTVNPRLFDEQLVYIINHAEDRVLFFDAAFL
CCCEEEEEEECCCCEEEEEECCCCCCCEEECCCCCHHCCEEEEEEECCCCEEEEEEHHHH
PIVERLRGQLTSVEHFVLFDASAQGDYKSYRDLVDAEDGDFEWVELGERDPVGLCYTSGT
HHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCEEEEECCC
TGNPKGVLYEHRSNVIHAITEIQPDAFDLSSRSVILPIVPMFHANSWGIPFAAATVGAKL
CCCCCCEEECCCCCCEEHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCCCHHHHHHCCEE
VFSATNDAQTLCDLMHAEGVTHSAGVPTVWLAMFAHMDATGMGYGALKHVIIGGSAAPRA
EEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHEEECCCCCHHH
MIERFMKAGVYVGHAWGMTETSPIGTMGKRPWNWDALSFDEQVDIICRQGCPPFGVELRI
HHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEE
VDDEGKELPRDGQTSGRLQIRGPWIIQRYFKADADAADADGWFDTGDVSVLHPDGVMQIT
ECCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHH
DRAKDVIKSGGEWISSIELENAAVGAPGVQEAAAVGVYHPKWDERPILLIVKKPGAEVSE
HHHHHHHHCCCHHHHHEEECCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHH
AAIIEYLKDKVAKWWLPDEVVFVDELPHTATGKILKRQIRDDYKDYKLKSLAEA
HHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]