Click here to switch to the map view.

The map label for this gene is dnaG [H]

Identifier: 103486469

GI number: 103486469

Start: 1005177

End: 1007054

Strand: Reverse

Name: dnaG [H]

Synonym: Sala_0980

Alternate gene names: 103486469

Gene position: 1007054-1005177 (Counterclockwise)

Preceding gene: 103486470

Following gene: 103486468

Centisome position: 30.1

GC content: 67.68

Gene sequence:

>1878_bases
ATGACCCTCACCCCGCAATGGCTTGACGAACTGCGCTCGCGGATCACGCTGTCGACCCTCATCGGGCGCACTGTGAAGAT
CACGCGCGCGGGCCGCGAGTATAAGGCCTGCTGCCCCTTCCATAACGAAAAGACGCCCAGCTTCACGATCAACGATGAAA
AGGGCTTCTATCACTGCTTCGGCTGCGGCGCGCATGGCGATGCGATCCGCTGGATGACCGACCAGCGCGGCCTCAGCTTC
ATGGATGCGGTGAAGGAGCTTGCCGCCGAAGCGGGGATGGAGGTTCCCGCCCCCGACCCGCGTGCCGCGAAAAAGGCCGA
AGAACAGGCAAGTCTGCGCGACGTGATGCAGGCCGCGGCCGAATGGTTCGCGCAGCAGCTTGGCAGCAGCAACGGCGCCC
CGGCGCGCGAATATCTCGCCAAGCGGGGTATTTCGGAAGCGACGCGCAAGGCCTTCGGCTTCGGCCTTGCGCCCGACAGC
CGCAGTGCGCTGAAGGAGGCGCTCAAGACGTTCCCGACCGCGATGCTCGTCGAGGCGGGGATGCTGATCGCCGTCGATGA
CAAGGAGCCCTACGACCGTTTCCGTGGGCGGCTGATGATCCCGATCCGTGATGCGCGCGGGCGCGTCATCGCCTTTGGCG
GACGCATCCTTGGCGACGGCGAGCCCAAATATCTGAACTCGCCCGACACGCCGCTCTTCGACAAGGGGCGCACGCTCTAC
AATCTCGACAAGGCAAGCCCCGCATCGCGGCGGACGAACCGCATCGTCGTCGTCGAAGGCTATATGGACGTCATCGCGCT
GGCGGAAGCGGGGATCACCGACGCGGTCGCGCCGCTCGGCACCGCGCTCACCGAGAACCAGCTCGCGATGCTGTGGCGCA
TGGTGCCCGTGCCCATCCTCTGTTTCGACGGCGACAGCGCGGGGCAGAAAGCGGCAATGCGCGCCGCGATGCGCGCGCTG
CCGCTGCTCCGCCCCGGCTTCAGCCTCGCCTTTGCGACGCTGCCGGCTGGGCAGGACCCCGACGACCTGGTGCGCGCCAA
GGGCGCCGAGGGCTTCGCGGCGATCCTGGAGGATGCGCAGCCGCTCGTCGAGCGCCTGTGGGCGCATGAGGTCGCCGCGG
GGCCGCTCGCGACGCCCGAGGAGCGGGCGGCGCTCAAGACGCGGCTGTTGGCGCACGCAGACGTGATCGAGGATGCCGAT
GTGCGCCACCATTATCGCGAGGCGTTCCGCGAAAAACTCGACGCGCTGTTCGCAAGGGCGCCCGCCGGGCGCGGCCCGCG
CGTGCCATGGGCGCCGCAGCCGGGGCGCGGCGGTCGGCGCTTCGCGCCCGACCCGCGGCTCCAGCCGCCGGGCGAGGAGA
CACGCGCGCTCGGCCAGACGGGCATCGCGGGCCCGCTCGCCGCGGCGTTGCTCGGCGGCCTGCTCCGCTATCCGCAAGCG
ATCCGGCGCAACGAAGAGGCGCTGTGCCGCCTCGCCATCCCCGATTCTGGCGACGCCGAACTGCTCGGGCTGATGCTTGA
CATCGCAAATGCCCGCGAAGGGCTTGATTGCGAGGGGTTGCTTGCCATATTGGAACCCATGAAAGTGTATAATAGGGCGA
TGACATTGCTCAGAGCCGATGGAATGCACTTCTCGTTCAATCGACGGCTCGAAGGCGAAGAGGTTGATAAGGCGCGCGAG
ATCGCGCTGCGCGACCTTGACGAATATATCGGCGTGCTCGTGACGCAGCCCGAAATCCGTGCCCGGCTGGCCGAGGCGAC
CGCGGATTATATGCGCACGATGGACGACGAAGGCCATGCGCGGCAGCAAAAGCTGCGCGCGATGGACGAGGATCTGACCC
GCCGCCTGGCCGCGCTGTCCGACAGCCGCAGCCAGTAA

Upstream 100 bases:

>100_bases
AAGTCGAGATGCCCGTCGAAATAGCGCCCAGCCCGATTGGTGTCTCGACTTCGCTCGACACGAGCGGGAATAGAGGCTTG
CCCAGTGGCGGAGGCAACCC

Downstream 100 bases:

>100_bases
CGACCCGTAAATGCCCCCGCAGGCAAGGAACAAGAATGGCCACGAAGAACGAAGCCGATACCGACGCCCCGCTGATCGAC
CTGAACGAGGCCGACGTCAA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MTLTPQWLDELRSRITLSTLIGRTVKITRAGREYKACCPFHNEKTPSFTINDEKGFYHCFGCGAHGDAIRWMTDQRGLSF
MDAVKELAAEAGMEVPAPDPRAAKKAEEQASLRDVMQAAAEWFAQQLGSSNGAPAREYLAKRGISEATRKAFGFGLAPDS
RSALKEALKTFPTAMLVEAGMLIAVDDKEPYDRFRGRLMIPIRDARGRVIAFGGRILGDGEPKYLNSPDTPLFDKGRTLY
NLDKASPASRRTNRIVVVEGYMDVIALAEAGITDAVAPLGTALTENQLAMLWRMVPVPILCFDGDSAGQKAAMRAAMRAL
PLLRPGFSLAFATLPAGQDPDDLVRAKGAEGFAAILEDAQPLVERLWAHEVAAGPLATPEERAALKTRLLAHADVIEDAD
VRHHYREAFREKLDALFARAPAGRGPRVPWAPQPGRGGRRFAPDPRLQPPGEETRALGQTGIAGPLAAALLGGLLRYPQA
IRRNEEALCRLAIPDSGDAELLGLMLDIANAREGLDCEGLLAILEPMKVYNRAMTLLRADGMHFSFNRRLEGEEVDKARE
IALRDLDEYIGVLVTQPEIRARLAEATADYMRTMDDEGHARQQKLRAMDEDLTRRLAALSDSRSQ

Sequences:

>Translated_625_residues
MTLTPQWLDELRSRITLSTLIGRTVKITRAGREYKACCPFHNEKTPSFTINDEKGFYHCFGCGAHGDAIRWMTDQRGLSF
MDAVKELAAEAGMEVPAPDPRAAKKAEEQASLRDVMQAAAEWFAQQLGSSNGAPAREYLAKRGISEATRKAFGFGLAPDS
RSALKEALKTFPTAMLVEAGMLIAVDDKEPYDRFRGRLMIPIRDARGRVIAFGGRILGDGEPKYLNSPDTPLFDKGRTLY
NLDKASPASRRTNRIVVVEGYMDVIALAEAGITDAVAPLGTALTENQLAMLWRMVPVPILCFDGDSAGQKAAMRAAMRAL
PLLRPGFSLAFATLPAGQDPDDLVRAKGAEGFAAILEDAQPLVERLWAHEVAAGPLATPEERAALKTRLLAHADVIEDAD
VRHHYREAFREKLDALFARAPAGRGPRVPWAPQPGRGGRRFAPDPRLQPPGEETRALGQTGIAGPLAAALLGGLLRYPQA
IRRNEEALCRLAIPDSGDAELLGLMLDIANAREGLDCEGLLAILEPMKVYNRAMTLLRADGMHFSFNRRLEGEEVDKARE
IALRDLDEYIGVLVTQPEIRARLAEATADYMRTMDDEGHARQQKLRAMDEDLTRRLAALSDSRSQ
>Mature_624_residues
TLTPQWLDELRSRITLSTLIGRTVKITRAGREYKACCPFHNEKTPSFTINDEKGFYHCFGCGAHGDAIRWMTDQRGLSFM
DAVKELAAEAGMEVPAPDPRAAKKAEEQASLRDVMQAAAEWFAQQLGSSNGAPAREYLAKRGISEATRKAFGFGLAPDSR
SALKEALKTFPTAMLVEAGMLIAVDDKEPYDRFRGRLMIPIRDARGRVIAFGGRILGDGEPKYLNSPDTPLFDKGRTLYN
LDKASPASRRTNRIVVVEGYMDVIALAEAGITDAVAPLGTALTENQLAMLWRMVPVPILCFDGDSAGQKAAMRAAMRALP
LLRPGFSLAFATLPAGQDPDDLVRAKGAEGFAAILEDAQPLVERLWAHEVAAGPLATPEERAALKTRLLAHADVIEDADV
RHHYREAFREKLDALFARAPAGRGPRVPWAPQPGRGGRRFAPDPRLQPPGEETRALGQTGIAGPLAAALLGGLLRYPQAI
RRNEEALCRLAIPDSGDAELLGLMLDIANAREGLDCEGLLAILEPMKVYNRAMTLLRADGMHFSFNRRLEGEEVDKAREI
ALRDLDEYIGVLVTQPEIRARLAEATADYMRTMDDEGHARQQKLRAMDEDLTRRLAALSDSRSQ

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=415, Percent_Identity=43.855421686747, Blast_Score=340, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 68541; Mature: 68410

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTPQWLDELRSRITLSTLIGRTVKITRAGREYKACCPFHNEKTPSFTINDEKGFYHCF
CCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCEEEEE
GCGAHGDAIRWMTDQRGLSFMDAVKELAAEAGMEVPAPDPRAAKKAEEQASLRDVMQAAA
ECCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EWFAQQLGSSNGAPAREYLAKRGISEATRKAFGFGLAPDSRSALKEALKTFPTAMLVEAG
HHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHCC
MLIAVDDKEPYDRFRGRLMIPIRDARGRVIAFGGRILGDGEPKYLNSPDTPLFDKGRTLY
CEEEECCCCHHHHHCCEEEEEEECCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCCEE
NLDKASPASRRTNRIVVVEGYMDVIALAEAGITDAVAPLGTALTENQLAMLWRMVPVPIL
ECCCCCCCCCCCCEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
CFDGDSAGQKAAMRAAMRALPLLRPGFSLAFATLPAGQDPDDLVRAKGAEGFAAILEDAQ
EECCCCCHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCHHHHHHHCCCCHHHHHHHHHH
PLVERLWAHEVAAGPLATPEERAALKTRLLAHADVIEDADVRHHYREAFREKLDALFARA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
PAGRGPRVPWAPQPGRGGRRFAPDPRLQPPGEETRALGQTGIAGPLAAALLGGLLRYPQA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IRRNEEALCRLAIPDSGDAELLGLMLDIANAREGLDCEGLLAILEPMKVYNRAMTLLRAD
HHCCCHHEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GMHFSFNRRLEGEEVDKAREIALRDLDEYIGVLVTQPEIRARLAEATADYMRTMDDEGHA
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHCCCCCHH
RQQKLRAMDEDLTRRLAALSDSRSQ
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TLTPQWLDELRSRITLSTLIGRTVKITRAGREYKACCPFHNEKTPSFTINDEKGFYHCF
CCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCEEECCCCCEEEEE
GCGAHGDAIRWMTDQRGLSFMDAVKELAAEAGMEVPAPDPRAAKKAEEQASLRDVMQAAA
ECCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
EWFAQQLGSSNGAPAREYLAKRGISEATRKAFGFGLAPDSRSALKEALKTFPTAMLVEAG
HHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHCC
MLIAVDDKEPYDRFRGRLMIPIRDARGRVIAFGGRILGDGEPKYLNSPDTPLFDKGRTLY
CEEEECCCCHHHHHCCEEEEEEECCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCCEE
NLDKASPASRRTNRIVVVEGYMDVIALAEAGITDAVAPLGTALTENQLAMLWRMVPVPIL
ECCCCCCCCCCCCEEEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEE
CFDGDSAGQKAAMRAAMRALPLLRPGFSLAFATLPAGQDPDDLVRAKGAEGFAAILEDAQ
EECCCCCHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCHHHHHHHCCCCHHHHHHHHHH
PLVERLWAHEVAAGPLATPEERAALKTRLLAHADVIEDADVRHHYREAFREKLDALFARA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
PAGRGPRVPWAPQPGRGGRRFAPDPRLQPPGEETRALGQTGIAGPLAAALLGGLLRYPQA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
IRRNEEALCRLAIPDSGDAELLGLMLDIANAREGLDCEGLLAILEPMKVYNRAMTLLRAD
HHCCCHHEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GMHFSFNRRLEGEEVDKAREIALRDLDEYIGVLVTQPEIRARLAEATADYMRTMDDEGHA
CCEEEECCCCCCHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHHHHHHHCCCCCHH
RQQKLRAMDEDLTRRLAALSDSRSQ
HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]