Click here to switch to the map view.

The map label for this gene is pyrB

Identifier: 103486414

GI number: 103486414

Start: 937479

End: 938513

Strand: Reverse

Name: pyrB

Synonym: Sala_0924

Alternate gene names: 103486414

Gene position: 938513-937479 (Counterclockwise)

Preceding gene: 103486415

Following gene: 103486413

Centisome position: 28.06

GC content: 65.7

Gene sequence:

>1035_bases
TTGCCAGAATCGCCGCCCCTACCTAAACGCTCCCCTTTAATGACAAGCTCCACCACCCGACCCGCCAGCGACTATCCGCC
CGGCGGCGATGCGTTTCGCCATCGCCACCTGACCGGTATCGCCCAGCTCACCCCGTGGGAGATTTCCTACATATTGGACG
CGGCCGAAGAATGGGTCGAACTCAATCGCAGCGGCGCCGCCAAGCATGACGACCGGCTCGCCGGGCTGACGATCATCAAC
GCCTTTTTCGAAAACTCGACGCGCACCTTGCTTTCGTTCGAAATCGCCGGAAAACGCCTCGGCGCCGACGTCGTCAACAT
GCACGCCGCGCAGTCGAGCGTGAAGAAGGGCGAGACGCTGATCGACACCGCGATGACCTTGAACGCGATGCGCGCCGACG
CCATCGTCATCCGCCACGCAAGTTCGGGCGCGGTGCAGCTGATCGCCGACAAGGTCGATTGTCCCGTGCTCAACGCGGGC
GACGGGCGCCACGAGCATCCGACGCAGGCGCTGCTCGACGCGCTGACGATCCGCCGCCGCCTCGGCCGCGTCGAGGGGCT
GGCCATTGCGATCTGCGGCGACGTCCTCCACAGCCGCGTCGCGCGCTCGAACATCCTCGCGCTCACCCTGCTCGGCAATG
AGGTGCGCGTCGTCGCGCCCGCGACGCTGACACCGCCCGCGATGGAACGGATGCACGTGCGCTGCTTCACCGACATGGAC
GAAGGGTTGAAAGGCGCCGACGTCGTGATGATGCTCCGCCTCCAGAACGAGCGCATGGATGGCGCCCATCTCCCCTCTGC
GCGCGAATATCACGCGCTCTATGGCCTGACCCCCCAGCGGCTCGAAAAGGCGAAGCCCGACGCCATCGTCATGCACCCCG
GTCCGATGAACCGCGGGGTGGAGATCGACAGCAGCGTCGCCGACGATCACGCGCGCTCGACGATCACCGAACAGGTCGAA
ATGGGGGTCGCGGTGCGCATGGCATGTCTCGACATATTGACGCGCCGCCAGCGGGGAGTGCCGGGATGGAATTGA

Upstream 100 bases:

>100_bases
AGCGCTGACGGAGGGGCCACAACGCTGCCGCTGCTGCCCCTCCACCACGCCCTGCGGGCGCGGGCCCCCTCCCCATGGCT
TCGCCACTGGGAGGATTGGC

Downstream 100 bases:

>100_bases
GGCCGCTCCATATCGCCAACGCGCTGCTGGTCGATGGCGATACGCCGCGTCCGGGCAGCCTGCTGGCGGTCGACGGCCGC
ATCGCCGCGATCGACCCGGC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MPESPPLPKRSPLMTSSTTRPASDYPPGGDAFRHRHLTGIAQLTPWEISYILDAAEEWVELNRSGAAKHDDRLAGLTIIN
AFFENSTRTLLSFEIAGKRLGADVVNMHAAQSSVKKGETLIDTAMTLNAMRADAIVIRHASSGAVQLIADKVDCPVLNAG
DGRHEHPTQALLDALTIRRRLGRVEGLAIAICGDVLHSRVARSNILALTLLGNEVRVVAPATLTPPAMERMHVRCFTDMD
EGLKGADVVMMLRLQNERMDGAHLPSAREYHALYGLTPQRLEKAKPDAIVMHPGPMNRGVEIDSSVADDHARSTITEQVE
MGVAVRMACLDILTRRQRGVPGWN

Sequences:

>Translated_344_residues
MPESPPLPKRSPLMTSSTTRPASDYPPGGDAFRHRHLTGIAQLTPWEISYILDAAEEWVELNRSGAAKHDDRLAGLTIIN
AFFENSTRTLLSFEIAGKRLGADVVNMHAAQSSVKKGETLIDTAMTLNAMRADAIVIRHASSGAVQLIADKVDCPVLNAG
DGRHEHPTQALLDALTIRRRLGRVEGLAIAICGDVLHSRVARSNILALTLLGNEVRVVAPATLTPPAMERMHVRCFTDMD
EGLKGADVVMMLRLQNERMDGAHLPSAREYHALYGLTPQRLEKAKPDAIVMHPGPMNRGVEIDSSVADDHARSTITEQVE
MGVAVRMACLDILTRRQRGVPGWN
>Mature_343_residues
PESPPLPKRSPLMTSSTTRPASDYPPGGDAFRHRHLTGIAQLTPWEISYILDAAEEWVELNRSGAAKHDDRLAGLTIINA
FFENSTRTLLSFEIAGKRLGADVVNMHAAQSSVKKGETLIDTAMTLNAMRADAIVIRHASSGAVQLIADKVDCPVLNAGD
GRHEHPTQALLDALTIRRRLGRVEGLAIAICGDVLHSRVARSNILALTLLGNEVRVVAPATLTPPAMERMHVRCFTDMDE
GLKGADVVMMLRLQNERMDGAHLPSAREYHALYGLTPQRLEKAKPDAIVMHPGPMNRGVEIDSSVADDHARSTITEQVEM
GVAVRMACLDILTRRQRGVPGWN

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=348, Percent_Identity=36.4942528735632, Blast_Score=178, Evalue=8e-45,
Organism=Escherichia coli, GI2367364, Length=314, Percent_Identity=33.4394904458599, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1790703, Length=232, Percent_Identity=30.1724137931034, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1786469, Length=228, Percent_Identity=28.5087719298246, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=291, Percent_Identity=35.7388316151203, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322331, Length=319, Percent_Identity=36.9905956112853, Blast_Score=154, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24642586, Length=271, Percent_Identity=39.4833948339483, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_SPHAL (Q1GUN0)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615975.1
- ProteinModelPortal:   Q1GUN0
- SMR:   Q1GUN0
- GeneID:   4080477
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0924
- NMPDR:   fig|317655.9.peg.864
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   SALA317655:SALA_0924-MONOMER
- BRENDA:   2.1.3.2
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 37543; Mature: 37411

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESPPLPKRSPLMTSSTTRPASDYPPGGDAFRHRHLTGIAQLTPWEISYILDAAEEWVE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LNRSGAAKHDDRLAGLTIINAFFENSTRTLLSFEIAGKRLGADVVNMHAAQSSVKKGETL
HCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHCHHHHHHHHHHHHHHCCCHH
IDTAMTLNAMRADAIVIRHASSGAVQLIADKVDCPVLNAGDGRHEHPTQALLDALTIRRR
HHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHHHH
LGRVEGLAIAICGDVLHSRVARSNILALTLLGNEVRVVAPATLTPPAMERMHVRCFTDMD
HCCCCCEEEEHHHHHHHHHHHHCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHEECCHH
EGLKGADVVMMLRLQNERMDGAHLPSAREYHALYGLTPQRLEKAKPDAIVMHPGPMNRGV
CCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCC
EIDSSVADDHARSTITEQVEMGVAVRMACLDILTRRQRGVPGWN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PESPPLPKRSPLMTSSTTRPASDYPPGGDAFRHRHLTGIAQLTPWEISYILDAAEEWVE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LNRSGAAKHDDRLAGLTIINAFFENSTRTLLSFEIAGKRLGADVVNMHAAQSSVKKGETL
HCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCHHHCHHHHHHHHHHHHHHCCCHH
IDTAMTLNAMRADAIVIRHASSGAVQLIADKVDCPVLNAGDGRHEHPTQALLDALTIRRR
HHHHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCEECCCCCCCCCHHHHHHHHHHHHHH
LGRVEGLAIAICGDVLHSRVARSNILALTLLGNEVRVVAPATLTPPAMERMHVRCFTDMD
HCCCCCEEEEHHHHHHHHHHHHCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHEECCHH
EGLKGADVVMMLRLQNERMDGAHLPSAREYHALYGLTPQRLEKAKPDAIVMHPGPMNRGV
CCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHCCCHHHHHHCCCCEEEECCCCCCCCC
EIDSSVADDHARSTITEQVEMGVAVRMACLDILTRRQRGVPGWN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA