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The map label for this gene is thlA [H]

Identifier: 103486273

GI number: 103486273

Start: 787380

End: 788567

Strand: Reverse

Name: thlA [H]

Synonym: Sala_0781

Alternate gene names: 103486273

Gene position: 788567-787380 (Counterclockwise)

Preceding gene: 103486275

Following gene: 103486272

Centisome position: 23.57

GC content: 68.01

Gene sequence:

>1188_bases
ATGACCGCCACCGATCCCGTCGTTTTCCTCTCCTATGCGCGCACCCCGATGGGCAGCATGCAAGGCAGCCTGTCGGATGC
GAGCGCGACCGACCTCGGCGCGACCGCGGTGAAGGCGGCGGTCGAGCGCGCAGGCGTCAGCGGCGACGACATCGAGCGCA
TCTATATGGGCTGCGTCCTCCCCGCCGGGCTGGGTCAGGCGCCCGCGCGCCAGGCCGCGATCAAGGCAGGCCTGCCCAAA
TCGGTGCAGGCGACGACCGTGAACAAGGTCTGCGGTTCGGGGATGCAAACGGTCATCATGGGCGCCGAAGCGCTCGCCGC
GGGCAGCGTCGAGTTGATCGTCGCGGGTGGCATGGAGTCGATGACCAATGCACCCTATCTGCTCAAGAAGCATCGCTCGG
GCGCGCGCATCGGCCACGATACGGCCTATGACCATATGTTCCTCGACGGGCTGGAGGACGCCTATGACGCGGGCCGCGCA
ATGGGCACCTTTGCGCAGGACACCGCCGACGCCTATCAGCTCAGCCGCCAGGCGATGGACGATTACACGCTGACGTCGCT
GAGCCGCGCCAAGGCCGCGATCGCCGAAGGCGCCTTCGTCGCCGAAATCGCCCCGGTGACGATTTCGGGCCGCAGGGGCG
ACGTCGTCGTCGACACCGACGAAGCGCCGGGCAAGGCGATGCCCGACAAGATCCCGACGCTCAAGCCCGCCTTTGCCAAG
GACGGTACGATCACCGCGGCGACCAGTTCGTCGATTTCGGACGGCGCCGCCGCGGTCGTGCTGACGCGCCAGTCGGTCGC
CGACGCCAAGGGCGCAAAGCCCGTCGCGCGGCTCGTCGCGCACGCCGCGCACGCCCAGGAGCCCAAGGATTTCACCGTCG
CCCCCGTCGGTGCGATCAACAAGCTGCTCGCCAGGACTGGCTGGTCGATCGGCGACGTCGACCTGTTCGAGGTCAATGAA
GCGTTCGCGTGTGTGGCGATGTTCGCGATGCACGACCTGGGCATTCCGCACGACAAGATCAACGTCCACGGCGGCGCGAC
CGCGCTCGGTCATCCGATCGGCGCCAGCGGCACGCGCATCATCACCACGCTGATCGCGGCGCTGCAACGCCACGGCAAAA
AACGCGGCATCGCCAGCCTGTGCATCGGCGGCGGCGAAGCGACCGCGGTGGCGATCGAGCTGGTGTAA

Upstream 100 bases:

>100_bases
GATGAAACGCGCTTCCCATCTTGGCGCTGCCCCTTTCGCAATTGCAGCAAAGCCGCTAATCGGGCCTCCGACATTGCTTT
CCCTTACGGAGTCTTTTGCC

Downstream 100 bases:

>100_bases
ATTGGCCCGTGCCCCTGCAAAGGCAGGGGCGCGGCTGTGGTCAGCGGCGACAGTCGGGCGCGGTGCTTCCCATCCCTATT
CCCGCGACACTTTGCGACAC

Product: Acetyl-CoA C-acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MTATDPVVFLSYARTPMGSMQGSLSDASATDLGATAVKAAVERAGVSGDDIERIYMGCVLPAGLGQAPARQAAIKAGLPK
SVQATTVNKVCGSGMQTVIMGAEALAAGSVELIVAGGMESMTNAPYLLKKHRSGARIGHDTAYDHMFLDGLEDAYDAGRA
MGTFAQDTADAYQLSRQAMDDYTLTSLSRAKAAIAEGAFVAEIAPVTISGRRGDVVVDTDEAPGKAMPDKIPTLKPAFAK
DGTITAATSSSISDGAAAVVLTRQSVADAKGAKPVARLVAHAAHAQEPKDFTVAPVGAINKLLARTGWSIGDVDLFEVNE
AFACVAMFAMHDLGIPHDKINVHGGATALGHPIGASGTRIITTLIAALQRHGKKRGIASLCIGGGEATAVAIELV

Sequences:

>Translated_395_residues
MTATDPVVFLSYARTPMGSMQGSLSDASATDLGATAVKAAVERAGVSGDDIERIYMGCVLPAGLGQAPARQAAIKAGLPK
SVQATTVNKVCGSGMQTVIMGAEALAAGSVELIVAGGMESMTNAPYLLKKHRSGARIGHDTAYDHMFLDGLEDAYDAGRA
MGTFAQDTADAYQLSRQAMDDYTLTSLSRAKAAIAEGAFVAEIAPVTISGRRGDVVVDTDEAPGKAMPDKIPTLKPAFAK
DGTITAATSSSISDGAAAVVLTRQSVADAKGAKPVARLVAHAAHAQEPKDFTVAPVGAINKLLARTGWSIGDVDLFEVNE
AFACVAMFAMHDLGIPHDKINVHGGATALGHPIGASGTRIITTLIAALQRHGKKRGIASLCIGGGEATAVAIELV
>Mature_394_residues
TATDPVVFLSYARTPMGSMQGSLSDASATDLGATAVKAAVERAGVSGDDIERIYMGCVLPAGLGQAPARQAAIKAGLPKS
VQATTVNKVCGSGMQTVIMGAEALAAGSVELIVAGGMESMTNAPYLLKKHRSGARIGHDTAYDHMFLDGLEDAYDAGRAM
GTFAQDTADAYQLSRQAMDDYTLTSLSRAKAAIAEGAFVAEIAPVTISGRRGDVVVDTDEAPGKAMPDKIPTLKPAFAKD
GTITAATSSSISDGAAAVVLTRQSVADAKGAKPVARLVAHAAHAQEPKDFTVAPVGAINKLLARTGWSIGDVDLFEVNEA
FACVAMFAMHDLGIPHDKINVHGGATALGHPIGASGTRIITTLIAALQRHGKKRGIASLCIGGGEATAVAIELV

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=393, Percent_Identity=42.7480916030534, Blast_Score=322, Evalue=5e-88,
Organism=Homo sapiens, GI4557237, Length=389, Percent_Identity=43.7017994858612, Blast_Score=302, Evalue=4e-82,
Organism=Homo sapiens, GI167614485, Length=389, Percent_Identity=36.2467866323907, Blast_Score=251, Evalue=6e-67,
Organism=Homo sapiens, GI4501853, Length=381, Percent_Identity=34.3832020997375, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=31.1163895486936, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI194353979, Length=88, Percent_Identity=46.5909090909091, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1788554, Length=388, Percent_Identity=47.1649484536082, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI87082165, Length=388, Percent_Identity=42.5257731958763, Blast_Score=308, Evalue=5e-85,
Organism=Escherichia coli, GI1787663, Length=392, Percent_Identity=35.7142857142857, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=35.8024691358025, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788683, Length=411, Percent_Identity=28.4671532846715, Blast_Score=134, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17535921, Length=389, Percent_Identity=42.159383033419, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI133906874, Length=390, Percent_Identity=40, Blast_Score=291, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI25147385, Length=381, Percent_Identity=40.6824146981627, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17535917, Length=387, Percent_Identity=34.1085271317829, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=421, Percent_Identity=29.4536817102138, Blast_Score=176, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6325229, Length=395, Percent_Identity=44.3037974683544, Blast_Score=322, Evalue=9e-89,
Organism=Saccharomyces cerevisiae, GI6322031, Length=337, Percent_Identity=33.2344213649852, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24655093, Length=388, Percent_Identity=44.0721649484536, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24640423, Length=389, Percent_Identity=45.7583547557841, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI17648125, Length=399, Percent_Identity=35.8395989974937, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI17137578, Length=421, Percent_Identity=29.2161520190024, Blast_Score=157, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40440; Mature: 40308

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00098 THIOLASE_1 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATDPVVFLSYARTPMGSMQGSLSDASATDLGATAVKAAVERAGVSGDDIERIYMGCVL
CCCCCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
PAGLGQAPARQAAIKAGLPKSVQATTVNKVCGSGMQTVIMGAEALAAGSVELIVAGGMES
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCHHEEHHCHHHHHCCCEEEEEECCHHH
MTNAPYLLKKHRSGARIGHDTAYDHMFLDGLEDAYDAGRAMGTFAQDTADAYQLSRQAMD
HCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
DYTLTSLSRAKAAIAEGAFVAEIAPVTISGRRGDVVVDTDEAPGKAMPDKIPTLKPAFAK
CHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
DGTITAATSSSISDGAAAVVLTRQSVADAKGAKPVARLVAHAAHAQEPKDFTVAPVGAIN
CCEEEEECCCCCCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEECHHHHH
KLLARTGWSIGDVDLFEVNEAFACVAMFAMHDLGIPHDKINVHGGATALGHPIGASGTRI
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHCCCCCCCCCHHH
ITTLIAALQRHGKKRGIASLCIGGGEATAVAIELV
HHHHHHHHHHCCHHCCCHHEEECCCCEEEEEEEEC
>Mature Secondary Structure 
TATDPVVFLSYARTPMGSMQGSLSDASATDLGATAVKAAVERAGVSGDDIERIYMGCVL
CCCCCEEEEEECCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHC
PAGLGQAPARQAAIKAGLPKSVQATTVNKVCGSGMQTVIMGAEALAAGSVELIVAGGMES
CCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCHHEEHHCHHHHHCCCEEEEEECCHHH
MTNAPYLLKKHRSGARIGHDTAYDHMFLDGLEDAYDAGRAMGTFAQDTADAYQLSRQAMD
HCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHC
DYTLTSLSRAKAAIAEGAFVAEIAPVTISGRRGDVVVDTDEAPGKAMPDKIPTLKPAFAK
CHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCC
DGTITAATSSSISDGAAAVVLTRQSVADAKGAKPVARLVAHAAHAQEPKDFTVAPVGAIN
CCEEEEECCCCCCCCCEEEEEEHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEECHHHHH
KLLARTGWSIGDVDLFEVNEAFACVAMFAMHDLGIPHDKINVHGGATALGHPIGASGTRI
HHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHCCCCCCCCCHHH
ITTLIAALQRHGKKRGIASLCIGGGEATAVAIELV
HHHHHHHHHHCCHHCCCHHEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]