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The map label for this gene is rlmN

Identifier: 103486196

GI number: 103486196

Start: 709125

End: 710387

Strand: Reverse

Name: rlmN

Synonym: Sala_0703

Alternate gene names: 103486196

Gene position: 710387-709125 (Counterclockwise)

Preceding gene: 103486198

Following gene: 103486195

Centisome position: 21.24

GC content: 65.16

Gene sequence:

>1263_bases
ATGCAGATTCCCGGCCATATCGACCCCGTCACGACGGGCACCGTCCCGCTGCGCGGGGGCAATCGTATCGACCTGGTCGG
GCTCAGCCGCGATGCGATCGGCGGTGTGCTGGTCGAGGCAGGACTCGACGCGAAGGCGGCGAAGCTGCGCGCCAAGCAGA
TCTGGCACTGGATCTATCATCGCGGCGTCACCGATTTCATGGGCATGACCGACATCGCCAAGGCGATGCGCCCCTGGCTG
ACCGACCGTTTCATCATCGGTCGCCCGACAGTGCGCGAGGCGCAGGTGTCGAGCGACGGCACGCGCAAATGGCTGCTCGC
CGCCGCCGACGGGCAGGAATATGAAATGGTGTTCATCCCCGACGCCGACCGGGGAACCTTGTGCGTGTCGAGCCAGGTCG
GGTGCACATTGAACTGCCGTTTCTGCCACACCGGCACGATGCGCCTCGTCCGCAACCTGGGCGCGGGCGAAATCGTCGGG
CAGGTGCTGCTGGCGCGCGACGCGCTCGGCGAATGGCCCAAGGGCAATATGGCGGGTTTTGGGGCCGGCTCCGACGCCGA
CCCGGAGGACGACGACGCCGACGACGATGCGGTCGGCCATTACACCGCCGACGGGCGGATGCTCACCAACATCGTGATGA
TGGGCATGGGCGAGCCGCTCTACAATTTCGACGAGGTCAAGGCCGCGCTGAAGATCGTTATGGACGGCGACGGGCTGGCC
CTGTCGAAGCGACGCATTACGCTGTCGACCAGCGGCGTCGTGCCGATGATGGCGCGCGCGGGCGAGGAGATCGGGGTCAA
TCTGGCGGTCTCGCTGCACGCGGTGAGCAAGGAAATCCGCGACGAGATTGTGCCGCTCAACCGCAAATATGGCATCGAGG
AATTGCTGCAGGCGTGCGCCGATTATCCGGGCGCGAACAATGCGCGGCGCATCACCTTTGAATATGTGATGCTGAAGGAC
AAGAATGACCGCGACGAGGATGCGCGCGAACTGGTGCGGCTGATCAAGCAGTATAAGCTCCCTGCAAAGGTCAACCTGAT
TCCCTTCAACCCCTGGCCCGGCGCGCCTTATGAATGTTCGACGCCCGAGCGGGTGCGCGCGTTCAGCAACCTGATTTTCA
AGGCGGGAATCTCCGCGCCGATCCGCACCCCGCGCGGGCGCGACATCATGGCGGCGTGCGGGCAATTGAAGAGCGCCGCG
ACCAGGCCGACGCGCGCCGAACTCGACCGCATCGCCGAGGAAAAGCAGGCAGCGCTGGGCTGA

Upstream 100 bases:

>100_bases
CGGACCTTGCAGGTTACGATCCTCCCCATCGCTTCGCGACAGGGAGGATTCTCGCGCGCAATTGCGCTTTTCTCCCTTCC
CCACTATATCGCGCCGCATC

Downstream 100 bases:

>100_bases
ACGCCCGCTCCTTCATCAACATACCTCCAGCAAGAACGTCGCTTTTCCAAGGGATTGGCCGCAACGTCCCTGCATAGCCA
TTCTATACCGTGTGGTAAAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MQIPGHIDPVTTGTVPLRGGNRIDLVGLSRDAIGGVLVEAGLDAKAAKLRAKQIWHWIYHRGVTDFMGMTDIAKAMRPWL
TDRFIIGRPTVREAQVSSDGTRKWLLAAADGQEYEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLGAGEIVG
QVLLARDALGEWPKGNMAGFGAGSDADPEDDDADDDAVGHYTADGRMLTNIVMMGMGEPLYNFDEVKAALKIVMDGDGLA
LSKRRITLSTSGVVPMMARAGEEIGVNLAVSLHAVSKEIRDEIVPLNRKYGIEELLQACADYPGANNARRITFEYVMLKD
KNDRDEDARELVRLIKQYKLPAKVNLIPFNPWPGAPYECSTPERVRAFSNLIFKAGISAPIRTPRGRDIMAACGQLKSAA
TRPTRAELDRIAEEKQAALG

Sequences:

>Translated_420_residues
MQIPGHIDPVTTGTVPLRGGNRIDLVGLSRDAIGGVLVEAGLDAKAAKLRAKQIWHWIYHRGVTDFMGMTDIAKAMRPWL
TDRFIIGRPTVREAQVSSDGTRKWLLAAADGQEYEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLGAGEIVG
QVLLARDALGEWPKGNMAGFGAGSDADPEDDDADDDAVGHYTADGRMLTNIVMMGMGEPLYNFDEVKAALKIVMDGDGLA
LSKRRITLSTSGVVPMMARAGEEIGVNLAVSLHAVSKEIRDEIVPLNRKYGIEELLQACADYPGANNARRITFEYVMLKD
KNDRDEDARELVRLIKQYKLPAKVNLIPFNPWPGAPYECSTPERVRAFSNLIFKAGISAPIRTPRGRDIMAACGQLKSAA
TRPTRAELDRIAEEKQAALG
>Mature_420_residues
MQIPGHIDPVTTGTVPLRGGNRIDLVGLSRDAIGGVLVEAGLDAKAAKLRAKQIWHWIYHRGVTDFMGMTDIAKAMRPWL
TDRFIIGRPTVREAQVSSDGTRKWLLAAADGQEYEMVFIPDADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLGAGEIVG
QVLLARDALGEWPKGNMAGFGAGSDADPEDDDADDDAVGHYTADGRMLTNIVMMGMGEPLYNFDEVKAALKIVMDGDGLA
LSKRRITLSTSGVVPMMARAGEEIGVNLAVSLHAVSKEIRDEIVPLNRKYGIEELLQACADYPGANNARRITFEYVMLKD
KNDRDEDARELVRLIKQYKLPAKVNLIPFNPWPGAPYECSTPERVRAFSNLIFKAGISAPIRTPRGRDIMAACGQLKSAA
TRPTRAELDRIAEEKQAALG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=389, Percent_Identity=43.9588688946015, Blast_Score=293, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_SPHAL (Q1GV98)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615757.1
- ProteinModelPortal:   Q1GV98
- GeneID:   4081792
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0703
- NMPDR:   fig|317655.9.peg.651
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   CLSK835312
- BioCyc:   SALA317655:SALA_0703-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 45880; Mature: 45880

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIPGHIDPVTTGTVPLRGGNRIDLVGLSRDAIGGVLVEAGLDAKAAKLRAKQIWHWIYH
CCCCCCCCCCCCCEEEECCCCEEEEEECCCHHHCCEEEECCCCHHHHHHHHHHHHHHHHH
RGVTDFMGMTDIAKAMRPWLTDRFIIGRPTVREAQVSSDGTRKWLLAAADGQEYEMVFIP
CCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHCCCCCCEEEEEEECCCCCEEEEEEC
DADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLGAGEIVGQVLLARDALGEWPKGNMAGF
CCCCCCEEEECCCCEEEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
GAGSDADPEDDDADDDAVGHYTADGRMLTNIVMMGMGEPLYNFDEVKAALKIVMDGDGLA
CCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCCCHHHHHHHEEEEECCCCCE
LSKRRITLSTSGVVPMMARAGEEIGVNLAVSLHAVSKEIRDEIVPLNRKYGIEELLQACA
EECEEEEEECCCCHHHHHHCCHHCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
DYPGANNARRITFEYVMLKDKNDRDEDARELVRLIKQYKLPAKVNLIPFNPWPGAPYECS
CCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
TPERVRAFSNLIFKAGISAPIRTPRGRDIMAACGQLKSAATRPTRAELDRIAEEKQAALG
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQIPGHIDPVTTGTVPLRGGNRIDLVGLSRDAIGGVLVEAGLDAKAAKLRAKQIWHWIYH
CCCCCCCCCCCCCEEEECCCCEEEEEECCCHHHCCEEEECCCCHHHHHHHHHHHHHHHHH
RGVTDFMGMTDIAKAMRPWLTDRFIIGRPTVREAQVSSDGTRKWLLAAADGQEYEMVFIP
CCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHCCCCCCEEEEEEECCCCCEEEEEEC
DADRGTLCVSSQVGCTLNCRFCHTGTMRLVRNLGAGEIVGQVLLARDALGEWPKGNMAGF
CCCCCCEEEECCCCEEEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCC
GAGSDADPEDDDADDDAVGHYTADGRMLTNIVMMGMGEPLYNFDEVKAALKIVMDGDGLA
CCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCCCHHHHHHHEEEEECCCCCE
LSKRRITLSTSGVVPMMARAGEEIGVNLAVSLHAVSKEIRDEIVPLNRKYGIEELLQACA
EECEEEEEECCCCHHHHHHCCHHCCCEEEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
DYPGANNARRITFEYVMLKDKNDRDEDARELVRLIKQYKLPAKVNLIPFNPWPGAPYECS
CCCCCCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
TPERVRAFSNLIFKAGISAPIRTPRGRDIMAACGQLKSAATRPTRAELDRIAEEKQAALG
CHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA