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The map label for this gene is rpoA

Identifier: 103486058

GI number: 103486058

Start: 578579

End: 579637

Strand: Reverse

Name: rpoA

Synonym: Sala_0565

Alternate gene names: 103486058

Gene position: 579637-578579 (Counterclockwise)

Preceding gene: 103486059

Following gene: 103486057

Centisome position: 17.33

GC content: 61.85

Gene sequence:

>1059_bases
ATGACTGTCAACATCCGGAACTGGCAGGAATTGAAGAAGCCCAGCAACCTGGAAATCAAGACCGGCGGCGACGGCAAGCG
CAAGGCGACCTTCGTTGCCGAACCGCTCGAGCGCGGTTTCGGCCTGACGCTGGGTAACGCGCTGCGCCGCGTGCTGCTCT
CGTCGCTCCAGGGCGCGGCGATCACCTCGATCAAGATCGAAAATGTGCTGCACGAGTTTTCGAGCCTTGCCGGTGTGCGC
GAAGACGTCACCGACATCGTCCTGAACGTCAAGCAGATCGCGCTCAAGATGGAGGGCGAAGGGCCGAAGCGGCTCCAGCT
TTCGGCGACGGGCCCGGCGACGGTCAAGGCGGGCGACATCATGGTGTCGGGCGACATCAAGGTGATGAACCCGAACCATG
TCATCTGCCATCTCGACGAAGGCGCGACGCTCAACATGGAGCTCGTCGCCGACACCGGCAAAGGCTATGTCCCCGCGACC
GCCAACCGCCCGGCCGATGCGCCGATCGGCCTGATCCCGGTCGACTCGCTCTATTCGCCGGTGCGCCAGGTCGCCTACAA
GGTCGACAATGCCCGCATCGGGCAGGAACTGGACTATGACAAGCTGAACCTGACCGTCGAAACCGACGGCACGGTGACGC
CCGAAGATGCTGTCGCTTACGCCGCACGCATCCTGCAGGATCAGCTCCAGGTCTTCGTCCACTTCGAAGAAGCGATGAGC
GACAGCGGCCTGATCGGCATGGCGGCCCCCTCGGCCGCCAGCGACGAGTCCGACGTCAACCAGCTCAACCGCTTCCTGCT
CAAGAAGGTCGACGAGCTTGAACTGTCGGTCCGCTCGGCCAACTGCCTGAAGAACGACAACATCATCTATATCGGTGACC
TCGTTCAAAAGACCGAAGCCGAAATGCTCCGCACGCCGAACTTCGGCCGCAAGTCCTTGAACGAAATCAAGGAAGTGCTG
TCGTCGATGGGCCTGCGTCTGGGCATGGACATCCCCGGCTGGCCACCCGAGAATATCGAGGAAATGGCCAAGAAGCTGGA
GCAGGAGCTGCTGGGTTAA

Upstream 100 bases:

>100_bases
CTTTCGGGCGCGCTCCAACACCGGAGCGCCGCCCGACCAGACCTCCTCGCTGGACCGATGGCCGACCCCCATCCGTCCCG
CCACTAGCAAAGGGAAATCC

Downstream 100 bases:

>100_bases
AAACCAAACCTTCTCCCGTCGGGAGAAGGATACGCAGCCTTGCCGACTGGTCGGCTAGGCGAAGTTGGATGAGGGGTTCC
GCGTTCTCGCGACAGGGCCG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MTVNIRNWQELKKPSNLEIKTGGDGKRKATFVAEPLERGFGLTLGNALRRVLLSSLQGAAITSIKIENVLHEFSSLAGVR
EDVTDIVLNVKQIALKMEGEGPKRLQLSATGPATVKAGDIMVSGDIKVMNPNHVICHLDEGATLNMELVADTGKGYVPAT
ANRPADAPIGLIPVDSLYSPVRQVAYKVDNARIGQELDYDKLNLTVETDGTVTPEDAVAYAARILQDQLQVFVHFEEAMS
DSGLIGMAAPSAASDESDVNQLNRFLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVL
SSMGLRLGMDIPGWPPENIEEMAKKLEQELLG

Sequences:

>Translated_352_residues
MTVNIRNWQELKKPSNLEIKTGGDGKRKATFVAEPLERGFGLTLGNALRRVLLSSLQGAAITSIKIENVLHEFSSLAGVR
EDVTDIVLNVKQIALKMEGEGPKRLQLSATGPATVKAGDIMVSGDIKVMNPNHVICHLDEGATLNMELVADTGKGYVPAT
ANRPADAPIGLIPVDSLYSPVRQVAYKVDNARIGQELDYDKLNLTVETDGTVTPEDAVAYAARILQDQLQVFVHFEEAMS
DSGLIGMAAPSAASDESDVNQLNRFLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVL
SSMGLRLGMDIPGWPPENIEEMAKKLEQELLG
>Mature_351_residues
TVNIRNWQELKKPSNLEIKTGGDGKRKATFVAEPLERGFGLTLGNALRRVLLSSLQGAAITSIKIENVLHEFSSLAGVRE
DVTDIVLNVKQIALKMEGEGPKRLQLSATGPATVKAGDIMVSGDIKVMNPNHVICHLDEGATLNMELVADTGKGYVPATA
NRPADAPIGLIPVDSLYSPVRQVAYKVDNARIGQELDYDKLNLTVETDGTVTPEDAVAYAARILQDQLQVFVHFEEAMSD
SGLIGMAAPSAASDESDVNQLNRFLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLS
SMGLRLGMDIPGWPPENIEEMAKKLEQELLG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=334, Percent_Identity=46.4071856287425, Blast_Score=297, Evalue=9e-82,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_SPHAL (Q1GVN6)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615619.1
- ProteinModelPortal:   Q1GVN6
- GeneID:   4081455
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0565
- NMPDR:   fig|317655.9.peg.523
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   SALA317655:SALA_0565-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 38362; Mature: 38231

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVNIRNWQELKKPSNLEIKTGGDGKRKATFVAEPLERGFGLTLGNALRRVLLSSLQGAA
CEECCCCHHHHCCCCCEEEEECCCCCCCHHEEHHHHHCCCCCHHHHHHHHHHHHHCCCCE
ITSIKIENVLHEFSSLAGVREDVTDIVLNVKQIALKMEGEGPKRLQLSATGPATVKAGDI
EEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCEEEECCE
MVSGDIKVMNPNHVICHLDEGATLNMELVADTGKGYVPATANRPADAPIGLIPVDSLYSP
EEECCEEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHH
VRQVAYKVDNARIGQELDYDKLNLTVETDGTVTPEDAVAYAARILQDQLQVFVHFEEAMS
HHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DSGLIGMAAPSAASDESDVNQLNRFLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTEA
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHH
EMLRTPNFGRKSLNEIKEVLSSMGLRLGMDIPGWPPENIEEMAKKLEQELLG
HHHCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVNIRNWQELKKPSNLEIKTGGDGKRKATFVAEPLERGFGLTLGNALRRVLLSSLQGAA
EECCCCHHHHCCCCCEEEEECCCCCCCHHEEHHHHHCCCCCHHHHHHHHHHHHHCCCCE
ITSIKIENVLHEFSSLAGVREDVTDIVLNVKQIALKMEGEGPKRLQLSATGPATVKAGDI
EEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEEECCCCCCEEEEECCCCCEEEECCE
MVSGDIKVMNPNHVICHLDEGATLNMELVADTGKGYVPATANRPADAPIGLIPVDSLYSP
EEECCEEEECCCEEEEEECCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHHHH
VRQVAYKVDNARIGQELDYDKLNLTVETDGTVTPEDAVAYAARILQDQLQVFVHFEEAMS
HHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
DSGLIGMAAPSAASDESDVNQLNRFLLKKVDELELSVRSANCLKNDNIIYIGDLVQKTEA
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHH
EMLRTPNFGRKSLNEIKEVLSSMGLRLGMDIPGWPPENIEEMAKKLEQELLG
HHHCCCCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA