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The map label for this gene is mdeA [H]

Identifier: 103485976

GI number: 103485976

Start: 499052

End: 500344

Strand: Reverse

Name: mdeA [H]

Synonym: Sala_0483

Alternate gene names: 103485976

Gene position: 500344-499052 (Counterclockwise)

Preceding gene: 103485977

Following gene: 103485974

Centisome position: 14.96

GC content: 64.19

Gene sequence:

>1293_bases
ATGGCCATGCGCGGCAAACCCAAGACGAATAGCAAAAAGATCGGCGACCGGGAGCTCAGCCCCGCGACGCTGATGATGGG
GCTCGGCTATGATCCCGTGCTGTCCGAAGGCTCGCTCAAACCCCCGATCTTCCTCACCTCGACCTTCGCCTTTGAAAGCG
CGGCGGCGGGCAAACGCTTCTTCGAACATATCACCGGCAAGCGCGAAGGCCCCGCCGATGGCCTCGTCTATTCGCGCTTC
AACGGTCCGAATCAGGAAATCCTCGAAGACCGGCTTGCGGTGTGGGACGGCGCCGACGACAGCCTCGTCTTTTCGAGCGG
CATGTCGGCGATCGCCACCCTGCTCCTCGCGCTGGTCGGCCAGAATGACGTCATCGTCCATTCGGGTCCGCTCTATGCCG
CCACCGAAACGCTGATCGCGCGTATCCTGTCGCGCTTCGGCGTGAGCTTCGTCGACTTTCCGGCGGGCGCATCGCGTGAC
GACATCGACGTCATTCTTGCGCGCGCGCAGGCGCTCGCCAGCCAGAAGGGCGGCAAGGTCGCGCTCGTCTATCTCGAAAG
CCCGGCGAACCCGACCAACGCGCTGGTCGATGTCGAGGCCGTCCGCGCTGCGCGCGACACCGCTTTTCCGGGCGAGCAAA
AGCCCGCGATCGCCATCGACAACACCTTCCTCGGCCCACTGTGGCAGCAGCCGCTGAAACAGGGCGCCGAACTCGTCATC
TACAGCCTCACCAAATATGCCGGCGGCCATTCGGACCTCGTCGCGGGCGGCGTGTCGGGCGACCAGCGGTTGCTCGACAT
CATCCGCCCGATGCGCAACACGATCGGTACGATCTGTGACCCGAATACCGCTTGGATGCTGCTGCGCAGCCTCGAAACGC
TCGAACTGCGCATGCGCCGCGCGGGCGAAAATGCGCTGAAGGTCTGCACCTTCCTTCGCGATCATCCGAAAGTCGAGGGG
CTCGGCTATCTCGGCTTCATCAGCGATGCGCGGCAGAAGGATATTTTCGACCGCCACTGCACCGGCGCCGGATCGACCTT
CTCGCTCTTCATCAAGGGCGGCGAGGCCGAGAGCTTCCGTTTCCTCGACGCCTTGCAGATCGCCAAGCTCGCGGTCAGCC
TGGGGGGCACGGAAACGCTCGCCAGCCATCCCGCCGCAATGACGCACCTGTCGGTGCCCGACGAACGCAAAAAAGCGCTC
GGCATCACCGACAATCTGGTGCGCGTGTCGATCGGCATCGAAGACCCCGACGATCTGATCGCCGACTTCGCGCAGGCGCT
GGACGCGGTTTGA

Upstream 100 bases:

>100_bases
GTGATCTGGGCGATCGCGGCGATCGCCTGACCCCGCGCAATATAGTTTCAAACGAAACTTGGTGCGCTATAAGGACCGCA
CCATCATCAGGAGCAGTTTC

Downstream 100 bases:

>100_bases
GAGGGGCTGGTTGGGGGGGGATGGCCGGAAACGACCGGTTACGGCCATAAGTTCCTCCCCATCGACTTGCGATGGGGAGG
TGGCAGCCCGCAGGGCTGAC

Product: methionine gamma-lyase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MAMRGKPKTNSKKIGDRELSPATLMMGLGYDPVLSEGSLKPPIFLTSTFAFESAAAGKRFFEHITGKREGPADGLVYSRF
NGPNQEILEDRLAVWDGADDSLVFSSGMSAIATLLLALVGQNDVIVHSGPLYAATETLIARILSRFGVSFVDFPAGASRD
DIDVILARAQALASQKGGKVALVYLESPANPTNALVDVEAVRAARDTAFPGEQKPAIAIDNTFLGPLWQQPLKQGAELVI
YSLTKYAGGHSDLVAGGVSGDQRLLDIIRPMRNTIGTICDPNTAWMLLRSLETLELRMRRAGENALKVCTFLRDHPKVEG
LGYLGFISDARQKDIFDRHCTGAGSTFSLFIKGGEAESFRFLDALQIAKLAVSLGGTETLASHPAAMTHLSVPDERKKAL
GITDNLVRVSIGIEDPDDLIADFAQALDAV

Sequences:

>Translated_430_residues
MAMRGKPKTNSKKIGDRELSPATLMMGLGYDPVLSEGSLKPPIFLTSTFAFESAAAGKRFFEHITGKREGPADGLVYSRF
NGPNQEILEDRLAVWDGADDSLVFSSGMSAIATLLLALVGQNDVIVHSGPLYAATETLIARILSRFGVSFVDFPAGASRD
DIDVILARAQALASQKGGKVALVYLESPANPTNALVDVEAVRAARDTAFPGEQKPAIAIDNTFLGPLWQQPLKQGAELVI
YSLTKYAGGHSDLVAGGVSGDQRLLDIIRPMRNTIGTICDPNTAWMLLRSLETLELRMRRAGENALKVCTFLRDHPKVEG
LGYLGFISDARQKDIFDRHCTGAGSTFSLFIKGGEAESFRFLDALQIAKLAVSLGGTETLASHPAAMTHLSVPDERKKAL
GITDNLVRVSIGIEDPDDLIADFAQALDAV
>Mature_429_residues
AMRGKPKTNSKKIGDRELSPATLMMGLGYDPVLSEGSLKPPIFLTSTFAFESAAAGKRFFEHITGKREGPADGLVYSRFN
GPNQEILEDRLAVWDGADDSLVFSSGMSAIATLLLALVGQNDVIVHSGPLYAATETLIARILSRFGVSFVDFPAGASRDD
IDVILARAQALASQKGGKVALVYLESPANPTNALVDVEAVRAARDTAFPGEQKPAIAIDNTFLGPLWQQPLKQGAELVIY
SLTKYAGGHSDLVAGGVSGDQRLLDIIRPMRNTIGTICDPNTAWMLLRSLETLELRMRRAGENALKVCTFLRDHPKVEGL
GYLGFISDARQKDIFDRHCTGAGSTFSLFIKGGEAESFRFLDALQIAKLAVSLGGTETLASHPAAMTHLSVPDERKKALG
ITDNLVRVSIGIEDPDDLIADFAQALDAV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=412, Percent_Identity=34.4660194174757, Blast_Score=202, Evalue=7e-52,
Organism=Homo sapiens, GI299473759, Length=412, Percent_Identity=32.0388349514563, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI25453487, Length=410, Percent_Identity=30.4878048780488, Blast_Score=148, Evalue=1e-35,
Organism=Escherichia coli, GI1790375, Length=411, Percent_Identity=33.5766423357664, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789383, Length=417, Percent_Identity=28.537170263789, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17538055, Length=391, Percent_Identity=36.3171355498721, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17560056, Length=389, Percent_Identity=34.1902313624679, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI71980708, Length=391, Percent_Identity=30.690537084399, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6319307, Length=397, Percent_Identity=34.5088161209068, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=435, Percent_Identity=28.5057471264368, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6321904, Length=398, Percent_Identity=30.1507537688442, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321254, Length=408, Percent_Identity=25.9803921568627, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321170, Length=351, Percent_Identity=23.6467236467236, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19922554, Length=393, Percent_Identity=37.6590330788804, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46159; Mature: 46028

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMRGKPKTNSKKIGDRELSPATLMMGLGYDPVLSEGSLKPPIFLTSTFAFESAAAGKRF
CCCCCCCCCCCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
FEHITGKREGPADGLVYSRFNGPNQEILEDRLAVWDGADDSLVFSSGMSAIATLLLALVG
HHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEHHCCHHHHHHHHHHHHC
QNDVIVHSGPLYAATETLIARILSRFGVSFVDFPAGASRDDIDVILARAQALASQKGGKV
CCCEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCE
ALVYLESPANPTNALVDVEAVRAARDTAFPGEQKPAIAIDNTFLGPLWQQPLKQGAELVI
EEEEEECCCCCCHHEEEHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCEEE
YSLTKYAGGHSDLVAGGVSGDQRLLDIIRPMRNTIGTICDPNTAWMLLRSLETLELRMRR
EEHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AGENALKVCTFLRDHPKVEGLGYLGFISDARQKDIFDRHCTGAGSTFSLFIKGGEAESFR
CCCHHHHHHHHHHCCCCCCCCEEEEHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHH
FLDALQIAKLAVSLGGTETLASHPAAMTHLSVPDERKKALGITDNLVRVSIGIEDPDDLI
HHHHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCHHHHHCCCCCCEEEEEECCCCHHHHH
ADFAQALDAV
HHHHHHHCCC
>Mature Secondary Structure 
AMRGKPKTNSKKIGDRELSPATLMMGLGYDPVLSEGSLKPPIFLTSTFAFESAAAGKRF
CCCCCCCCCCCCCCCCCCCCHHEEEECCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHH
FEHITGKREGPADGLVYSRFNGPNQEILEDRLAVWDGADDSLVFSSGMSAIATLLLALVG
HHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCEEHHCCHHHHHHHHHHHHC
QNDVIVHSGPLYAATETLIARILSRFGVSFVDFPAGASRDDIDVILARAQALASQKGGKV
CCCEEEECCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCE
ALVYLESPANPTNALVDVEAVRAARDTAFPGEQKPAIAIDNTFLGPLWQQPLKQGAELVI
EEEEEECCCCCCHHEEEHHHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHCCCCEEE
YSLTKYAGGHSDLVAGGVSGDQRLLDIIRPMRNTIGTICDPNTAWMLLRSLETLELRMRR
EEHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
AGENALKVCTFLRDHPKVEGLGYLGFISDARQKDIFDRHCTGAGSTFSLFIKGGEAESFR
CCCHHHHHHHHHHCCCCCCCCEEEEHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCHHH
FLDALQIAKLAVSLGGTETLASHPAAMTHLSVPDERKKALGITDNLVRVSIGIEDPDDLI
HHHHHHHHHHHHHCCCCHHHHCCCCCEEECCCCCHHHHHCCCCCCEEEEEECCCCHHHHH
ADFAQALDAV
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]