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The map label for this gene is grpE [H]

Identifier: 103485895

GI number: 103485895

Start: 410461

End: 410997

Strand: Reverse

Name: grpE [H]

Synonym: Sala_0401

Alternate gene names: 103485895

Gene position: 410997-410461 (Counterclockwise)

Preceding gene: 103485896

Following gene: 103485886

Centisome position: 12.29

GC content: 58.47

Gene sequence:

>537_bases
GTGTCGGACAATGCAAAGAATGGCGAGAACGAGGACGGATCGCCCTCGGCAGTTGGTAAAAGCGTGGAGTCGGAGGTCCT
GCCGGAACCGGAAGAAGGGGACGCGTTACAGGATCGATTGCTTCGTGCACTGGCGGAAGCTGAGAATGCGCGAAAGCGCG
CAGAAAAAGCGGGAACCGAAGGTCGACAGGCCGGGATGGCGCAACTGCTATCCGAACTCGCACCAGCGCTCGACAATCTC
GACCTTGCCTTGGAGGCCTTGGCGGGTTCACACGAGATCGAGCCATCGTTGATCAAGGGGCTGGAGGCGACGCGGCGAGC
AATCAACGATGCCTTGCTCAAGGCCGGGGTAAAAATACTGCGTCCTTCGATTGGCGAAGATCCTGATCCCAACGTGCATG
AAATTATCGGGACCGTGCCGAGTACGGGATCCAATGCGGGCCAGATCGTGAGCGTCGCGCAGTCAGGTTACATGATTGGG
CCCAGACTGGTTCGCGCCGCGAGAGTCATTGTTTCACGAGAAGTGGATGCTTCCTGA

Upstream 100 bases:

>100_bases
GAGCTGCCGGTTCGGAAGCTTCGGATGATGAAGAAGTCGTCGACGCCGAATTCTCCGAGGTCGATGATGACAAGAAAGAC
TAAGTAGGGGGGTACAGGCC

Downstream 100 bases:

>100_bases
CGTGACATGACCAGCCTCTTTCGGCGCGGAACCAGTGGCCAAGCGAAGAGGCCTGGAGACAGACCGCGCTATAACGGCGA
GCTGATAGCGAAGACATGGT

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MSDNAKNGENEDGSPSAVGKSVESEVLPEPEEGDALQDRLLRALAEAENARKRAEKAGTEGRQAGMAQLLSELAPALDNL
DLALEALAGSHEIEPSLIKGLEATRRAINDALLKAGVKILRPSIGEDPDPNVHEIIGTVPSTGSNAGQIVSVAQSGYMIG
PRLVRAARVIVSREVDAS

Sequences:

>Translated_178_residues
MSDNAKNGENEDGSPSAVGKSVESEVLPEPEEGDALQDRLLRALAEAENARKRAEKAGTEGRQAGMAQLLSELAPALDNL
DLALEALAGSHEIEPSLIKGLEATRRAINDALLKAGVKILRPSIGEDPDPNVHEIIGTVPSTGSNAGQIVSVAQSGYMIG
PRLVRAARVIVSREVDAS
>Mature_177_residues
SDNAKNGENEDGSPSAVGKSVESEVLPEPEEGDALQDRLLRALAEAENARKRAEKAGTEGRQAGMAQLLSELAPALDNLD
LALEALAGSHEIEPSLIKGLEATRRAINDALLKAGVKILRPSIGEDPDPNVHEIIGTVPSTGSNAGQIVSVAQSGYMIGP
RLVRAARVIVSREVDAS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI40255109, Length=148, Percent_Identity=31.0810810810811, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1788967, Length=137, Percent_Identity=32.1167883211679, Blast_Score=78, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=179, Percent_Identity=31.2849162011173, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 18621; Mature: 18490

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNAKNGENEDGSPSAVGKSVESEVLPEPEEGDALQDRLLRALAEAENARKRAEKAGTE
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GRQAGMAQLLSELAPALDNLDLALEALAGSHEIEPSLIKGLEATRRAINDALLKAGVKIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RPSIGEDPDPNVHEIIGTVPSTGSNAGQIVSVAQSGYMIGPRLVRAARVIVSREVDAS
CCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDNAKNGENEDGSPSAVGKSVESEVLPEPEEGDALQDRLLRALAEAENARKRAEKAGTE
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GRQAGMAQLLSELAPALDNLDLALEALAGSHEIEPSLIKGLEATRRAINDALLKAGVKIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RPSIGEDPDPNVHEIIGTVPSTGSNAGQIVSVAQSGYMIGPRLVRAARVIVSREVDAS
CCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA