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The map label for this gene is gshB [H]

Identifier: 103485756

GI number: 103485756

Start: 262302

End: 263255

Strand: Reverse

Name: gshB [H]

Synonym: Sala_0261

Alternate gene names: 103485756

Gene position: 263255-262302 (Counterclockwise)

Preceding gene: 103485757

Following gene: 103485755

Centisome position: 7.87

GC content: 65.72

Gene sequence:

>954_bases
ATGACCCTGCGCGCCGCGGTGCAAATGGATCCGATGGACGGTATCAACATCGCGGGCGACAGCAGCTTCGCCCTGATGCT
GAAGGGGCTCGAACGCGGTTACACCCTCTATCATTACGACGTCCGCGGCCTGACGTGGGAGGCCGGGCGGCTGACGACCA
AGGCGCACGGCGTGGTGGCGGCGCAGCGCGTCGCGGGCGATCATTACCGCTTCGGCGACGAAGTCACGCTCGACCTCGGC
CGCGACGTCGACGTCGTACTGATGCGGCAGGACCCGCCCTTCGACCTCGGCTATCTCACCGGCACCTGGCTGCTCGAACG
CATCCAGGGCGAAACCCTGGTGGTCAACGACCCCGTGTCGGTGCGCAACGCGCCCGAAAAGGTCTTCGTGCTCGATTTCC
CCGAATATATGCCGCCGACGATGGTGACGCGCGATCTCGACGCAGTAAAGGATTTCCAGGCGCGCCACGGCGCCGTCGTC
ATCAAGCCGCTCCACGGCAACGGCGGCAAGGCGGTGTTCCGCATCGACGCCGACGGCGGCAACCTCGGCGCGCTCGTCGA
ACTGTTCGGACAGGTGTGGCCCGAACCCTTCATGGTCCAGCAGTTCCTGCCCAGCGTTGCCCAGGGCGACAAGCGCATCG
TGCTTGTCGACGGCGAAGTCGCAGGCGCAATCAACCGCAAGCCCGGCGAGGGCGAATTCCGCTCGAACCTTGCGGTCGGC
GGTTATGCGGAAGCTGCCGAGCTCACCCCGCGCGAACAGGAAATCTGCGCCGCGCTCGCTCCCGCGCTCAAGGAACGCGG
GCTGATCTTTGTCGGCATCGACGTCATCGGCGGCGAATGGCTGACCGAAATCAACGTCACCTCGCCCACCGGCATTGTCG
CGATCGACCGTTTCAACAACAGCGACACCGCGGGAATGATCTGGGACGCGATCGCGCGGCGGATCGGCAGGTGA

Upstream 100 bases:

>100_bases
GCCGGTGCGTCCGATTGCGACGACGAGATCCCGGCCTTCGCCGGGATGACGAATCAGGATAGGCGTGCAGCCTAATGGCG
CGCGCTGTGAGGAGAAAAAA

Downstream 100 bases:

>100_bases
ACAATTGCTTCGTCATTGCGAGGACCCCGGACTTGATCCGGGGGACGAAGCAATCTCCAGCCATCGTCCGAGTGCTACGC
AAGCTGGAGATTGCTTCGCT

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MTLRAAVQMDPMDGINIAGDSSFALMLKGLERGYTLYHYDVRGLTWEAGRLTTKAHGVVAAQRVAGDHYRFGDEVTLDLG
RDVDVVLMRQDPPFDLGYLTGTWLLERIQGETLVVNDPVSVRNAPEKVFVLDFPEYMPPTMVTRDLDAVKDFQARHGAVV
IKPLHGNGGKAVFRIDADGGNLGALVELFGQVWPEPFMVQQFLPSVAQGDKRIVLVDGEVAGAINRKPGEGEFRSNLAVG
GYAEAAELTPREQEICAALAPALKERGLIFVGIDVIGGEWLTEINVTSPTGIVAIDRFNNSDTAGMIWDAIARRIGR

Sequences:

>Translated_317_residues
MTLRAAVQMDPMDGINIAGDSSFALMLKGLERGYTLYHYDVRGLTWEAGRLTTKAHGVVAAQRVAGDHYRFGDEVTLDLG
RDVDVVLMRQDPPFDLGYLTGTWLLERIQGETLVVNDPVSVRNAPEKVFVLDFPEYMPPTMVTRDLDAVKDFQARHGAVV
IKPLHGNGGKAVFRIDADGGNLGALVELFGQVWPEPFMVQQFLPSVAQGDKRIVLVDGEVAGAINRKPGEGEFRSNLAVG
GYAEAAELTPREQEICAALAPALKERGLIFVGIDVIGGEWLTEINVTSPTGIVAIDRFNNSDTAGMIWDAIARRIGR
>Mature_316_residues
TLRAAVQMDPMDGINIAGDSSFALMLKGLERGYTLYHYDVRGLTWEAGRLTTKAHGVVAAQRVAGDHYRFGDEVTLDLGR
DVDVVLMRQDPPFDLGYLTGTWLLERIQGETLVVNDPVSVRNAPEKVFVLDFPEYMPPTMVTRDLDAVKDFQARHGAVVI
KPLHGNGGKAVFRIDADGGNLGALVELFGQVWPEPFMVQQFLPSVAQGDKRIVLVDGEVAGAINRKPGEGEFRSNLAVGG
YAEAAELTPREQEICAALAPALKERGLIFVGIDVIGGEWLTEINVTSPTGIVAIDRFNNSDTAGMIWDAIARRIGR

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=315, Percent_Identity=40.3174603174603, Blast_Score=221, Evalue=4e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34561; Mature: 34430

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRAAVQMDPMDGINIAGDSSFALMLKGLERGYTLYHYDVRGLTWEAGRLTTKAHGVVA
CCEEEEEEECCCCCCEECCCCCHHHEEHHHHCCEEEEEEEECCEEEECCCEEEHHCCCEE
AQRVAGDHYRFGDEVTLDLGRDVDVVLMRQDPPFDLGYLTGTWLLERIQGETLVVNDPVS
HHHHCCCCEECCCEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCC
VRNAPEKVFVLDFPEYMPPTMVTRDLDAVKDFQARHGAVVIKPLHGNGGKAVFRIDADGG
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCC
NLGALVELFGQVWPEPFMVQQFLPSVAQGDKRIVLVDGEVAGAINRKPGEGEFRSNLAVG
CHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCCHHHCCEEEC
GYAEAAELTPREQEICAALAPALKERGLIFVGIDVIGGEWLTEINVTSPTGIVAIDRFNN
CCCCHHHCCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCEEEEEECCCCCCEEEEECCCC
SDTAGMIWDAIARRIGR
CCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TLRAAVQMDPMDGINIAGDSSFALMLKGLERGYTLYHYDVRGLTWEAGRLTTKAHGVVA
CEEEEEEECCCCCCEECCCCCHHHEEHHHHCCEEEEEEEECCEEEECCCEEEHHCCCEE
AQRVAGDHYRFGDEVTLDLGRDVDVVLMRQDPPFDLGYLTGTWLLERIQGETLVVNDPVS
HHHHCCCCEECCCEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEECCCC
VRNAPEKVFVLDFPEYMPPTMVTRDLDAVKDFQARHGAVVIKPLHGNGGKAVFRIDADGG
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEEECCCC
NLGALVELFGQVWPEPFMVQQFLPSVAQGDKRIVLVDGEVAGAINRKPGEGEFRSNLAVG
CHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCCEECCCCCCCCCCHHHCCEEEC
GYAEAAELTPREQEICAALAPALKERGLIFVGIDVIGGEWLTEINVTSPTGIVAIDRFNN
CCCCHHHCCCCHHHHHHHHHHHHHHCCEEEEEEEEECCCEEEEEECCCCCCEEEEECCCC
SDTAGMIWDAIARRIGR
CCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA