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The map label for this gene is grpE [H]

Identifier: 103485747

GI number: 103485747

Start: 255275

End: 255820

Strand: Reverse

Name: grpE [H]

Synonym: Sala_0252

Alternate gene names: 103485747

Gene position: 255820-255275 (Counterclockwise)

Preceding gene: 103485748

Following gene: 103485744

Centisome position: 7.65

GC content: 67.22

Gene sequence:

>546_bases
ATGGAAAATGACACGCCGGTCGACGACGGCCAGACCGACGACGCCGCAACCGAAACCGAAACCGCGGCGACCGAACCGCA
GGACGAGGCGGCGAAGCTCGCCGAACAGCTCGCCGCGGTGCAGCAGGATTTGCTCTATGCGCGCGCCGAGACGCAGAATG
TCCGCCGCCGCGCCGAGAAGGAAGTCGCCGACGCGCACGCCTATGCCGCGACCAGGTTCGCGCGCGATATCCTCTCGGTC
GCCGACAATCTGGGCCGCGCACTCGCTGCGCTGAGCGACGAACAGCGCGCGGACGAGGCGATCAAGCCGCTGGTGACGGG
GCTGGAAGCGACCGAGCGCGAACTGATGGCGGTGTTCGAACGCCATGGCATCACGCGCATCGCCGCGATCGGCCTGCCGC
TCGACCCCAACCAGCATCAGGCGATGCTCGAAATCCCCAGCGACAAGGAACCCGGCACGATCGTGCAGGAAATGCAGGCG
GGCTATATGATGAAGGACCGGCTGCTGCGGCCCGCCATGGTGGCGGTGGCGAAGAAGGCGGAGTAG

Upstream 100 bases:

>100_bases
GTGATCGGCCCGACGCGCTTGAACTATGCCCGCATCGTTCCCATGGTGGATTTCACCGCCCAATCGCTCTCCAGACTGAT
ACGATAGGTTTATGACGAAC

Downstream 100 bases:

>100_bases
CCAAATGCTCCCCTCCCTTTACGGGAGGGGATACAGGGGTGGGTGCCGCGGCGCGTCTGCGCCGCATAAAATCCCGAGAC
ATTGAGGTTCGGAAAACAGT

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MENDTPVDDGQTDDAATETETAATEPQDEAAKLAEQLAAVQQDLLYARAETQNVRRRAEKEVADAHAYAATRFARDILSV
ADNLGRALAALSDEQRADEAIKPLVTGLEATERELMAVFERHGITRIAAIGLPLDPNQHQAMLEIPSDKEPGTIVQEMQA
GYMMKDRLLRPAMVAVAKKAE

Sequences:

>Translated_181_residues
MENDTPVDDGQTDDAATETETAATEPQDEAAKLAEQLAAVQQDLLYARAETQNVRRRAEKEVADAHAYAATRFARDILSV
ADNLGRALAALSDEQRADEAIKPLVTGLEATERELMAVFERHGITRIAAIGLPLDPNQHQAMLEIPSDKEPGTIVQEMQA
GYMMKDRLLRPAMVAVAKKAE
>Mature_181_residues
MENDTPVDDGQTDDAATETETAATEPQDEAAKLAEQLAAVQQDLLYARAETQNVRRRAEKEVADAHAYAATRFARDILSV
ADNLGRALAALSDEQRADEAIKPLVTGLEATERELMAVFERHGITRIAAIGLPLDPNQHQAMLEIPSDKEPGTIVQEMQA
GYMMKDRLLRPAMVAVAKKAE

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=176, Percent_Identity=36.3636363636364, Blast_Score=107, Evalue=5e-24,
Organism=Homo sapiens, GI40255109, Length=145, Percent_Identity=32.4137931034483, Blast_Score=80, Evalue=9e-16,
Organism=Escherichia coli, GI1788967, Length=171, Percent_Identity=38.0116959064327, Blast_Score=101, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17552458, Length=136, Percent_Identity=36.7647058823529, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324806, Length=168, Percent_Identity=36.9047619047619, Blast_Score=113, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653432, Length=170, Percent_Identity=38.2352941176471, Blast_Score=110, Evalue=4e-25,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 19766; Mature: 19766

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENDTPVDDGQTDDAATETETAATEPQDEAAKLAEQLAAVQQDLLYARAETQNVRRRAEK
CCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVADAHAYAATRFARDILSVADNLGRALAALSDEQRADEAIKPLVTGLEATERELMAVFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHGITRIAAIGLPLDPNQHQAMLEIPSDKEPGTIVQEMQAGYMMKDRLLRPAMVAVAKKA
HCCCHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
E
C
>Mature Secondary Structure
MENDTPVDDGQTDDAATETETAATEPQDEAAKLAEQLAAVQQDLLYARAETQNVRRRAEK
CCCCCCCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVADAHAYAATRFARDILSVADNLGRALAALSDEQRADEAIKPLVTGLEATERELMAVFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHGITRIAAIGLPLDPNQHQAMLEIPSDKEPGTIVQEMQAGYMMKDRLLRPAMVAVAKKA
HCCCHHHHHCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA