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The map label for this gene is yfiN [H]

Identifier: 103485734

GI number: 103485734

Start: 243964

End: 245190

Strand: Reverse

Name: yfiN [H]

Synonym: Sala_0238

Alternate gene names: 103485734

Gene position: 245190-243964 (Counterclockwise)

Preceding gene: 103485735

Following gene: 103485733

Centisome position: 7.33

GC content: 67.07

Gene sequence:

>1227_bases
ATGAGCGGCCCCGACCTGCCCCGCCCGGTCGAACGGATCGGCGCGCGCACGACGCTGCAAAAGCTGCTGTCGCGCTTCCA
TTTCGGCATCACCCTGTTCGCGGTCGCGCTGTCGGGGCTGACGATCCTGCTCGCCGGCGTCACGGCGCTGCGCGGCTATG
CCGACCGCAACATGGAACTCGCGGCGCAGCTTGGCGCCTATGGCGTCGAACCCGCGCTGGTCTTCAACGACCCCGTCGCC
GCGCGCGAAGGGATCGAGCCGCTGACGCGCATTCCCGGCATCACGCGGCTGCGCGTCATCGACGATGTCGGCAGACCAGT
CATCGAATGGAAATCGCCTGCCGGAGATCCGGCGCCGCTGCTCAGCCGCACCTTCTTCCCGCGCCCCTTTGCGGTCACTG
TCCGGCGCAACGGATCGGCGATCGGCACGGTCGAGGTCTGGGGCGACAGTTCGACCCTGATCGACTATGTGCGGCTCGGC
CTGCTCGCCGGACTGGCCTGCCTCCTCGTCACCGCATTGGGGACGATCTTCCTCGCCCGCCGGTTCGAATATGAACTGGT
CAAGCCGCTGAACGAGATCGCGACGGTCGCGCACGACGTGCGCCTCCACCGCCGCTTCGACAAGCGCGTGAAGCCGCTCG
GCATTGCCGAACTCGACCGGCTCGGCGGCGACATCAATGCGCTGCTCGACGAGCTTCAGGGCTGGCAGGGGCATATGGAA
AGCGAACGCGCGCTGCTCGCGCACCGCGCGTCGCACGACACGCTGACCGCGCTGCCCAACCGTGCGGCTTTCGACGAACA
GCTTGCGCTGCGGATCGCTGCGGCAAAGGCGCGCGATGGCCGGTTCGCGCTGCTGTTCATCGATGCCGACAATTTCAAGG
CGGCGAACGACAATTTCGGCCATGCCGCAGGCGACGCCGTGCTTGTCGCGATATCGGCCTGCATCCGGAATACGCTGCGT
AAGGATGATTTCGCCGCGCGGATCGGCGGCGACGAGTTCGTCGTCATCATCGATCCCTTCGACGTCGGCGTCGTCCCGGC
CGATCTGGCGAACCGCCTGCGCGCCTGCATTGCCGACCCCATCACCCTGCCCGACGGCGAATCCTATCGTGCGTCGGTCA
GCGTCGGCGTCGCGGTCTTTCCCGACGATGGCGGCGACGCCACCGCGCTGCTCACCGCCGCCGATGCGGCGATGTATAGC
GAAAAGATGACTCATCGTTCCCGATAG

Upstream 100 bases:

>100_bases
CAAGCCTCAGCTTCTCGGTCAATCTCGACGCCATAGCGCGCGGTCCGCTCAAGATCGATCCGCGCGTGCTGCGCATCGGG
GCTGGCGATGGAGGCGCCGG

Downstream 100 bases:

>100_bases
ACCAGCGGAGAGAAGCCATGACCATCCCACTCGTCCGACCGTTATGTACGCTGTTCCTGACCGCTTTCGCCGCGCTGCTG
GCGGCGTGCCAGAGCCTGCC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MSGPDLPRPVERIGARTTLQKLLSRFHFGITLFAVALSGLTILLAGVTALRGYADRNMELAAQLGAYGVEPALVFNDPVA
AREGIEPLTRIPGITRLRVIDDVGRPVIEWKSPAGDPAPLLSRTFFPRPFAVTVRRNGSAIGTVEVWGDSSTLIDYVRLG
LLAGLACLLVTALGTIFLARRFEYELVKPLNEIATVAHDVRLHRRFDKRVKPLGIAELDRLGGDINALLDELQGWQGHME
SERALLAHRASHDTLTALPNRAAFDEQLALRIAAAKARDGRFALLFIDADNFKAANDNFGHAAGDAVLVAISACIRNTLR
KDDFAARIGGDEFVVIIDPFDVGVVPADLANRLRACIADPITLPDGESYRASVSVGVAVFPDDGGDATALLTAADAAMYS
EKMTHRSR

Sequences:

>Translated_408_residues
MSGPDLPRPVERIGARTTLQKLLSRFHFGITLFAVALSGLTILLAGVTALRGYADRNMELAAQLGAYGVEPALVFNDPVA
AREGIEPLTRIPGITRLRVIDDVGRPVIEWKSPAGDPAPLLSRTFFPRPFAVTVRRNGSAIGTVEVWGDSSTLIDYVRLG
LLAGLACLLVTALGTIFLARRFEYELVKPLNEIATVAHDVRLHRRFDKRVKPLGIAELDRLGGDINALLDELQGWQGHME
SERALLAHRASHDTLTALPNRAAFDEQLALRIAAAKARDGRFALLFIDADNFKAANDNFGHAAGDAVLVAISACIRNTLR
KDDFAARIGGDEFVVIIDPFDVGVVPADLANRLRACIADPITLPDGESYRASVSVGVAVFPDDGGDATALLTAADAAMYS
EKMTHRSR
>Mature_407_residues
SGPDLPRPVERIGARTTLQKLLSRFHFGITLFAVALSGLTILLAGVTALRGYADRNMELAAQLGAYGVEPALVFNDPVAA
REGIEPLTRIPGITRLRVIDDVGRPVIEWKSPAGDPAPLLSRTFFPRPFAVTVRRNGSAIGTVEVWGDSSTLIDYVRLGL
LAGLACLLVTALGTIFLARRFEYELVKPLNEIATVAHDVRLHRRFDKRVKPLGIAELDRLGGDINALLDELQGWQGHMES
ERALLAHRASHDTLTALPNRAAFDEQLALRIAAAKARDGRFALLFIDADNFKAANDNFGHAAGDAVLVAISACIRNTLRK
DDFAARIGGDEFVVIIDPFDVGVVPADLANRLRACIADPITLPDGESYRASVSVGVAVFPDDGGDATALLTAADAAMYSE
KMTHRSR

Specific function: Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HAMP domain [H]

Homologues:

Organism=Escherichia coli, GI1788956, Length=394, Percent_Identity=31.4720812182741, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1788381, Length=222, Percent_Identity=35.1351351351351, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1787541, Length=153, Percent_Identity=37.2549019607843, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87081977, Length=171, Percent_Identity=35.0877192982456, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=35.7575757575758, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=31.7365269461078, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=31.4814814814815, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081881, Length=170, Percent_Identity=35.2941176470588, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786584, Length=186, Percent_Identity=30.1075268817204, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787816, Length=174, Percent_Identity=30.4597701149425, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787802, Length=171, Percent_Identity=30.4093567251462, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081974, Length=159, Percent_Identity=27.6729559748428, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788085, Length=164, Percent_Identity=28.0487804878049, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 44046; Mature: 43915

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPDLPRPVERIGARTTLQKLLSRFHFGITLFAVALSGLTILLAGVTALRGYADRNMEL
CCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AAQLGAYGVEPALVFNDPVAAREGIEPLTRIPGITRLRVIDDVGRPVIEWKSPAGDPAPL
HHHHCCCCCCCEEEECCCHHHHHCCCHHHHCCCCEEEHHHHHCCCCEEECCCCCCCCHHH
LSRTFFPRPFAVTVRRNGSAIGTVEVWGDSSTLIDYVRLGLLAGLACLLVTALGTIFLAR
HHHCCCCCCEEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFEYELVKPLNEIATVAHDVRLHRRFDKRVKPLGIAELDRLGGDINALLDELQGWQGHME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHH
SERALLAHRASHDTLTALPNRAAFDEQLALRIAAAKARDGRFALLFIDADNFKAANDNFG
HHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCC
HAAGDAVLVAISACIRNTLRKDDFAARIGGDEFVVIIDPFDVGVVPADLANRLRACIADP
CCHHHHHHHHHHHHHHHHHCCCCHHEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCC
ITLPDGESYRASVSVGVAVFPDDGGDATALLTAADAAMYSEKMTHRSR
CCCCCCCCCEEEEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGPDLPRPVERIGARTTLQKLLSRFHFGITLFAVALSGLTILLAGVTALRGYADRNMEL
CCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AAQLGAYGVEPALVFNDPVAAREGIEPLTRIPGITRLRVIDDVGRPVIEWKSPAGDPAPL
HHHHCCCCCCCEEEECCCHHHHHCCCHHHHCCCCEEEHHHHHCCCCEEECCCCCCCCHHH
LSRTFFPRPFAVTVRRNGSAIGTVEVWGDSSTLIDYVRLGLLAGLACLLVTALGTIFLAR
HHHCCCCCCEEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFEYELVKPLNEIATVAHDVRLHRRFDKRVKPLGIAELDRLGGDINALLDELQGWQGHME
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHH
SERALLAHRASHDTLTALPNRAAFDEQLALRIAAAKARDGRFALLFIDADNFKAANDNFG
HHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCC
HAAGDAVLVAISACIRNTLRKDDFAARIGGDEFVVIIDPFDVGVVPADLANRLRACIADP
CCHHHHHHHHHHHHHHHHHCCCCHHEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCC
ITLPDGESYRASVSVGVAVFPDDGGDATALLTAADAAMYSEKMTHRSR
CCCCCCCCCEEEEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 6357787; 7567469 [H]