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The map label for this gene is mnmG

Identifier: 99082705

GI number: 99082705

Start: 3034009

End: 3035892

Strand: Direct

Name: mnmG

Synonym: TM1040_2865

Alternate gene names: 99082705

Gene position: 3034009-3035892 (Clockwise)

Preceding gene: 99082704

Following gene: 99082706

Centisome position: 94.78

GC content: 50.85

Gene sequence:

>1884_bases
GTGTTTCACGTGAAACATTCACATTATGACGTGGTTGTAGTTGGTGGTGGACATGCTGGAACCGAAGCGGCTCACGCGTC
CGCGAGGATGGGCGTGCGCACCGCACTGGTGACTTTGCGACGTGATGGTATCGGCGTCATGTCTTGCAACCCGGCGATTG
GCGGTCTTGGAAAAGGTCACCTGGTTCGAGAAATTGACGCCCTCGACGGCGTCATGGGGCGCGTAGCCGACCTCGCTGGT
ATTCAATTCAGGCTTCTCAACCGACGCAAGGGGCCAGCGGTCCAAGGGCCGCGCGCCCAGGCCGATCGCTCGATCTATCG
CAAGGAAATGTTGCGTGCGACAGAGGCACAATCAAATCTGGACATTATCGAAGGTGAGGTCGTTGATTTCCTGATGGAAT
CTCCGACTAGGGTCCGCGGCATCATACTCGACGATCAAAGTGAAATTCGTGCAGAATGCGTAGTTCTTACGTCAGGTACC
TTCTTGAATGGAGTTATCCATATTGGGAATAAGTCATCGTCAGGTGGTCGCATGGGAGACAGGCCCTCGATCAAACTTGC
CGAGCGCTTGAAATCCTTTGAACTGCCTTTAGGTCGGCTCAAGACCGGCACACCTCCGCGGCTGGACGGGAGGACGATCA
ACTGGGATATACTTGATAGTCAACCAGGTGATGAAGATCCTACTCTCTTCTCATTTCTGAGCACAAATACATCGGCACCC
CAAATCGCCTGCGGTATTACCCATACGAACGAGAAAACTCACGAAATTATCAGAGATAATCTCGAGCTGTCAGCTATGTA
TGGTGGGCATATAGAAGGCGTCGGGCCTCGCTACTGCCCGTCTATCGAAGACAAAATCGTTCGTTTTGCGGATAAGACTT
CGCATCAGATCTTTCTGGAACCCGAAGGCGTGAACGATCATACGGTTTACCCAAACGGCATATCAACGAGTTTACCTGAA
GATGTACAAGTGGACTACGTCCGATCAATAGTCGGCCTGGAAAATGCTGAAGTATTGCAACCTGGCTATGCGATTGAATA
CGACTACGTTGATCCGCGCGCGCTTAGATCAACGCTAGAACTTCGCGATGTCGAAGGGCTCTATTTGGCGGGTCAGATCA
ATGGCACCACTGGGTATGAAGAAGCCGCAGCTCAGGGTTTGGTGGCTGGACTCAACGCCGCGTCCCATAGATTGAAACAA
CAGGAAATAGTGTTTGGACGTTCGAACAGCTACATCGGAGTGATGATTGACGATCTCATCACAAATGGGGTGACGGAACC
CTATCGTATGTTTACGTCTCGTGCGGAGTTTAGATTGTCCCTGAGAGCCGACAATGCAGACCAAAGATTGACGCCTATCG
GGTATGACCTTGGTTGTATTTCTGAGGTAAGGAGAAGTGAATTTGATCGCAAAATGGATGCGATAACACGAACTAAGAGC
GCGCTTACCTCTGTACTTCTTACACCCAAACAACTTCTCGAGGCTGGTATCCAGGTGAATCAGGATGGGAACAAGCGGAA
CGGGCTAGATGTTCTCGCTTTTCCAAAAGTCGAATTTGATGACATTCTGCCTCTTTTTCCAGAACTAGCTGATACTGAGA
CTAGTATCCGCGAACAGGTGACGCGGGATTCTCTGTATGCGAACTATATCGCGCGACAGGAGAAGGAAGTCGCGTCATTG
AAGCGGGATGAACAATACAGAATTCCCCGTGATTTCAGCTATGAGATCGATGGGCTGTCCATGGAGTTACGGCAAAAGCT
TGAACGTTCGAGACCTGAGAGCTTAGCGCAAGCCGCTCGAATTGATGGTATGACCCCAGCCGCACTGGCGCTGATGCTTG
GAAGTTTGAAGAAGCTTTCCAGGGGCGAAAGCAAAGCATCATGA

Upstream 100 bases:

>100_bases
GAGATCGGCGATCCGAGCGCTAGAGTTGCTAGTGGGTCGGATAAATGTAGAGAATCTTCTGGATGAGATTTTCTCGAGTT
TCTGTTTGGGAAAGTAAGGA

Downstream 100 bases:

>100_bases
GCGATGGTCGAGGAAACTTGGAACTTGATGTTTCACGTGAAACATTAGAACGACTGATGGTCTTCTCAGAGGTCCTTAAA
AAGTGGAACCCGAAGATCAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MFHVKHSHYDVVVVGGGHAGTEAAHASARMGVRTALVTLRRDGIGVMSCNPAIGGLGKGHLVREIDALDGVMGRVADLAG
IQFRLLNRRKGPAVQGPRAQADRSIYRKEMLRATEAQSNLDIIEGEVVDFLMESPTRVRGIILDDQSEIRAECVVLTSGT
FLNGVIHIGNKSSSGGRMGDRPSIKLAERLKSFELPLGRLKTGTPPRLDGRTINWDILDSQPGDEDPTLFSFLSTNTSAP
QIACGITHTNEKTHEIIRDNLELSAMYGGHIEGVGPRYCPSIEDKIVRFADKTSHQIFLEPEGVNDHTVYPNGISTSLPE
DVQVDYVRSIVGLENAEVLQPGYAIEYDYVDPRALRSTLELRDVEGLYLAGQINGTTGYEEAAAQGLVAGLNAASHRLKQ
QEIVFGRSNSYIGVMIDDLITNGVTEPYRMFTSRAEFRLSLRADNADQRLTPIGYDLGCISEVRRSEFDRKMDAITRTKS
ALTSVLLTPKQLLEAGIQVNQDGNKRNGLDVLAFPKVEFDDILPLFPELADTETSIREQVTRDSLYANYIARQEKEVASL
KRDEQYRIPRDFSYEIDGLSMELRQKLERSRPESLAQAARIDGMTPAALALMLGSLKKLSRGESKAS

Sequences:

>Translated_627_residues
MFHVKHSHYDVVVVGGGHAGTEAAHASARMGVRTALVTLRRDGIGVMSCNPAIGGLGKGHLVREIDALDGVMGRVADLAG
IQFRLLNRRKGPAVQGPRAQADRSIYRKEMLRATEAQSNLDIIEGEVVDFLMESPTRVRGIILDDQSEIRAECVVLTSGT
FLNGVIHIGNKSSSGGRMGDRPSIKLAERLKSFELPLGRLKTGTPPRLDGRTINWDILDSQPGDEDPTLFSFLSTNTSAP
QIACGITHTNEKTHEIIRDNLELSAMYGGHIEGVGPRYCPSIEDKIVRFADKTSHQIFLEPEGVNDHTVYPNGISTSLPE
DVQVDYVRSIVGLENAEVLQPGYAIEYDYVDPRALRSTLELRDVEGLYLAGQINGTTGYEEAAAQGLVAGLNAASHRLKQ
QEIVFGRSNSYIGVMIDDLITNGVTEPYRMFTSRAEFRLSLRADNADQRLTPIGYDLGCISEVRRSEFDRKMDAITRTKS
ALTSVLLTPKQLLEAGIQVNQDGNKRNGLDVLAFPKVEFDDILPLFPELADTETSIREQVTRDSLYANYIARQEKEVASL
KRDEQYRIPRDFSYEIDGLSMELRQKLERSRPESLAQAARIDGMTPAALALMLGSLKKLSRGESKAS
>Mature_627_residues
MFHVKHSHYDVVVVGGGHAGTEAAHASARMGVRTALVTLRRDGIGVMSCNPAIGGLGKGHLVREIDALDGVMGRVADLAG
IQFRLLNRRKGPAVQGPRAQADRSIYRKEMLRATEAQSNLDIIEGEVVDFLMESPTRVRGIILDDQSEIRAECVVLTSGT
FLNGVIHIGNKSSSGGRMGDRPSIKLAERLKSFELPLGRLKTGTPPRLDGRTINWDILDSQPGDEDPTLFSFLSTNTSAP
QIACGITHTNEKTHEIIRDNLELSAMYGGHIEGVGPRYCPSIEDKIVRFADKTSHQIFLEPEGVNDHTVYPNGISTSLPE
DVQVDYVRSIVGLENAEVLQPGYAIEYDYVDPRALRSTLELRDVEGLYLAGQINGTTGYEEAAAQGLVAGLNAASHRLKQ
QEIVFGRSNSYIGVMIDDLITNGVTEPYRMFTSRAEFRLSLRADNADQRLTPIGYDLGCISEVRRSEFDRKMDAITRTKS
ALTSVLLTPKQLLEAGIQVNQDGNKRNGLDVLAFPKVEFDDILPLFPELADTETSIREQVTRDSLYANYIARQEKEVASL
KRDEQYRIPRDFSYEIDGLSMELRQKLERSRPESLAQAARIDGMTPAALALMLGSLKKLSRGESKAS

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family

Homologues:

Organism=Homo sapiens, GI74024895, Length=640, Percent_Identity=45.3125, Blast_Score=520, Evalue=1e-147,
Organism=Homo sapiens, GI19882217, Length=665, Percent_Identity=43.609022556391, Blast_Score=506, Evalue=1e-143,
Organism=Homo sapiens, GI183227703, Length=680, Percent_Identity=42.6470588235294, Blast_Score=501, Evalue=1e-142,
Organism=Escherichia coli, GI2367273, Length=620, Percent_Identity=52.741935483871, Blast_Score=625, Evalue=1e-180,
Organism=Caenorhabditis elegans, GI17534255, Length=617, Percent_Identity=42.3014586709887, Blast_Score=467, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6321202, Length=640, Percent_Identity=46.40625, Blast_Score=560, Evalue=1e-160,
Organism=Drosophila melanogaster, GI24658174, Length=631, Percent_Identity=45.1664025356577, Blast_Score=504, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMG_SILST (Q1GCL9)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614859.1
- ProteinModelPortal:   Q1GCL9
- SMR:   Q1GCL9
- STRING:   Q1GCL9
- GeneID:   4076399
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2865
- NMPDR:   fig|292414.1.peg.1768
- eggNOG:   COG0445
- HOGENOM:   HBG284774
- OMA:   GIQFRVL
- PhylomeDB:   Q1GCL9
- ProtClustDB:   PRK05192
- BioCyc:   SSP292414:TM1040_2865-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00129
- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00136

Pfam domain/function: PF01134 GIDA

EC number: NA

Molecular weight: Translated: 69082; Mature: 69082

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: BINDING 127-127 BINDING 183-183 BINDING 372-372

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHVKHSHYDVVVVGGGHAGTEAAHASARMGVRTALVTLRRDGIGVMSCNPAIGGLGKGH
CCEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCH
LVREIDALDGVMGRVADLAGIQFRLLNRRKGPAVQGPRAQADRSIYRKEMLRATEAQSNL
HHHHHHHHHHHHHHHHHHHCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
DIIEGEVVDFLMESPTRVRGIILDDQSEIRAECVVLTSGTFLNGVIHIGNKSSSGGRMGD
CCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCEEEEEEEECCCCCCCCCCCC
RPSIKLAERLKSFELPLGRLKTGTPPRLDGRTINWDILDSQPGDEDPTLFSFLSTNTSAP
CCCHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHEECCCCCCC
QIACGITHTNEKTHEIIRDNLELSAMYGGHIEGVGPRYCPSIEDKIVRFADKTSHQIFLE
EEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
PEGVNDHTVYPNGISTSLPEDVQVDYVRSIVGLENAEVLQPGYAIEYDYVDPRALRSTLE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCEEEECCCCHHHHHHHHH
LRDVEGLYLAGQINGTTGYEEAAAQGLVAGLNAASHRLKQQEIVFGRSNSYIGVMIDDLI
HCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCCEEEEEHHHHH
TNGVTEPYRMFTSRAEFRLSLRADNADQRLTPIGYDLGCISEVRRSEFDRKMDAITRTKS
HCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALTSVLLTPKQLLEAGIQVNQDGNKRNGLDVLAFPKVEFDDILPLFPELADTETSIREQV
HHHHHHCCHHHHHHCCCEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHH
TRDSLYANYIARQEKEVASLKRDEQYRIPRDFSYEIDGLSMELRQKLERSRPESLAQAAR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHCCHHHHHHHHH
IDGMTPAALALMLGSLKKLSRGESKAS
CCCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFHVKHSHYDVVVVGGGHAGTEAAHASARMGVRTALVTLRRDGIGVMSCNPAIGGLGKGH
CCEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCH
LVREIDALDGVMGRVADLAGIQFRLLNRRKGPAVQGPRAQADRSIYRKEMLRATEAQSNL
HHHHHHHHHHHHHHHHHHHCHHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
DIIEGEVVDFLMESPTRVRGIILDDQSEIRAECVVLTSGTFLNGVIHIGNKSSSGGRMGD
CCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEEEEECCCEEEEEEEECCCCCCCCCCCC
RPSIKLAERLKSFELPLGRLKTGTPPRLDGRTINWDILDSQPGDEDPTLFSFLSTNTSAP
CCCHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHEECCCCCCC
QIACGITHTNEKTHEIIRDNLELSAMYGGHIEGVGPRYCPSIEDKIVRFADKTSHQIFLE
EEEEEEECCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEE
PEGVNDHTVYPNGISTSLPEDVQVDYVRSIVGLENAEVLQPGYAIEYDYVDPRALRSTLE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEECCCCEEEECCCCHHHHHHHHH
LRDVEGLYLAGQINGTTGYEEAAAQGLVAGLNAASHRLKQQEIVFGRSNSYIGVMIDDLI
HCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCCEEEEEHHHHH
TNGVTEPYRMFTSRAEFRLSLRADNADQRLTPIGYDLGCISEVRRSEFDRKMDAITRTKS
HCCCCHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ALTSVLLTPKQLLEAGIQVNQDGNKRNGLDVLAFPKVEFDDILPLFPELADTETSIREQV
HHHHHHCCHHHHHHCCCEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHH
TRDSLYANYIARQEKEVASLKRDEQYRIPRDFSYEIDGLSMELRQKLERSRPESLAQAAR
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHCCHHHHHHHHH
IDGMTPAALALMLGSLKKLSRGESKAS
CCCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA