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The map label for this gene is trmE

Identifier: 99082704

GI number: 99082704

Start: 3032719

End: 3034005

Strand: Direct

Name: trmE

Synonym: TM1040_2864

Alternate gene names: 99082704

Gene position: 3032719-3034005 (Clockwise)

Preceding gene: 99082703

Following gene: 99082705

Centisome position: 94.74

GC content: 53.69

Gene sequence:

>1287_bases
ATGGAGACGATCTTTGCGTTGGCCACCGCCCAAGGAAAGGCCGGTGTCGCCGTTATACGTGTATCCGGTCCGCATGCGAT
CGAGATCGGCGAAAAACTAACGCGCAGGACGCTACCTGCGCGAGGTATGATATTTTCAAAATTGAAAGATAGCGCAGGTG
AGATCCTTGATGAGGCTCTGGTCTTGTCGTTTACGTCACCTGACAGCTTCACCGGCGAAAATATCGTAGAGTTTCAATTA
CATGGCAGCATAGCTGTCGTATCTGCAGTGATGTCTGCGATTGAGGAAACCGGTGTTGCGCGTCTTGCGGACCCCGGAGA
GTTTACCCGCCGGGCGTTGGACAACGGAAAGCTCGATCTAACTCAGGTTGAGGGCCTTGCTGATCTTATTGATGCGGAGA
CGGAAGCTCAAAGAAAACAGGCGCAGGTCATCCTTGCAGGCACACTGGGGGATCTGGCAGACCGTTGGCGAACAGATCTT
ATTCGTGCGGCGTCCCTGATTGAGGTTACAATCGATTTCGCGGACGAAGAAGTACCTGTGGATGTCACGCCAGAGGTAAA
GCAGCTTCTTTCTGGCGTATTGCACGACATTGAAAAAGAGGTCGATGGCGTTGCTATTGCCGAGCGCATTCGCGAGGGTT
TTGAAGTGGCTATTGTCGGCTCGCCCAATGTCGGAAAGTCGACGCTCCTCAATGCGCTTGCTGGGCGCACCGCAGCGATC
ACATCTGAGTATGCGGGAACAACGCGCGATGTGATCGAGGTTCGAATGGACCTCGCTGGCCTACCAGTTACCTTATTGGA
TACGGCCGGACTGAGAGAGACTGACGATCATGTTGAGGGGATTGGTATCCGTCTCGCGCAGGAGCGCGCAGAAAGGGCTG
ACATCCGCGTATTCCTGGCCGAAGAGGATGAGAGTTTCTCAATTCAGATGCGAGAAGGGGATCTCCGACTGCTTCCGAAG
GCGGATGAGCGACGGTCTGCGAAGGGCGCGATTTCTGGCAGAACAGGGCAGGGCGTAGATGACTTGGTATCGCGTATATC
GGATACCCTTAGAAATCGGACTGCCCATGATGGTATTGCGACAAGAGCACGGCATCGGGAGACGATGAACCGTGCTGTGC
TGAGTTTGCGCCAAGCGATTGAAGTCGTTGAACAGGGGCCCGAGTTTTATGACCTGGCAGCTGAGGACATGAGATCGGCG
ATCCGAGCGCTAGAGTTGCTAGTGGGTCGGATAAATGTAGAGAATCTTCTGGATGAGATTTTCTCGAGTTTCTGTTTGGG
AAAGTAA

Upstream 100 bases:

>100_bases
TGAGTTCCTGCTGTCTAAGTTGAAGCAAACAAAGACAAACTCAGAGTTTTTTGACTCGATGAACACCTAAACGGGGTAGG
GCGGAGAAAGAGGCCATCCC

Downstream 100 bases:

>100_bases
GGAGTGTTTCACGTGAAACATTCACATTATGACGTGGTTGTAGTTGGTGGTGGACATGCTGGAACCGAAGCGGCTCACGC
GTCCGCGAGGATGGGCGTGC

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
METIFALATAQGKAGVAVIRVSGPHAIEIGEKLTRRTLPARGMIFSKLKDSAGEILDEALVLSFTSPDSFTGENIVEFQL
HGSIAVVSAVMSAIEETGVARLADPGEFTRRALDNGKLDLTQVEGLADLIDAETEAQRKQAQVILAGTLGDLADRWRTDL
IRAASLIEVTIDFADEEVPVDVTPEVKQLLSGVLHDIEKEVDGVAIAERIREGFEVAIVGSPNVGKSTLLNALAGRTAAI
TSEYAGTTRDVIEVRMDLAGLPVTLLDTAGLRETDDHVEGIGIRLAQERAERADIRVFLAEEDESFSIQMREGDLRLLPK
ADERRSAKGAISGRTGQGVDDLVSRISDTLRNRTAHDGIATRARHRETMNRAVLSLRQAIEVVEQGPEFYDLAAEDMRSA
IRALELLVGRINVENLLDEIFSSFCLGK

Sequences:

>Translated_428_residues
METIFALATAQGKAGVAVIRVSGPHAIEIGEKLTRRTLPARGMIFSKLKDSAGEILDEALVLSFTSPDSFTGENIVEFQL
HGSIAVVSAVMSAIEETGVARLADPGEFTRRALDNGKLDLTQVEGLADLIDAETEAQRKQAQVILAGTLGDLADRWRTDL
IRAASLIEVTIDFADEEVPVDVTPEVKQLLSGVLHDIEKEVDGVAIAERIREGFEVAIVGSPNVGKSTLLNALAGRTAAI
TSEYAGTTRDVIEVRMDLAGLPVTLLDTAGLRETDDHVEGIGIRLAQERAERADIRVFLAEEDESFSIQMREGDLRLLPK
ADERRSAKGAISGRTGQGVDDLVSRISDTLRNRTAHDGIATRARHRETMNRAVLSLRQAIEVVEQGPEFYDLAAEDMRSA
IRALELLVGRINVENLLDEIFSSFCLGK
>Mature_428_residues
METIFALATAQGKAGVAVIRVSGPHAIEIGEKLTRRTLPARGMIFSKLKDSAGEILDEALVLSFTSPDSFTGENIVEFQL
HGSIAVVSAVMSAIEETGVARLADPGEFTRRALDNGKLDLTQVEGLADLIDAETEAQRKQAQVILAGTLGDLADRWRTDL
IRAASLIEVTIDFADEEVPVDVTPEVKQLLSGVLHDIEKEVDGVAIAERIREGFEVAIVGSPNVGKSTLLNALAGRTAAI
TSEYAGTTRDVIEVRMDLAGLPVTLLDTAGLRETDDHVEGIGIRLAQERAERADIRVFLAEEDESFSIQMREGDLRLLPK
ADERRSAKGAISGRTGQGVDDLVSRISDTLRNRTAHDGIATRARHRETMNRAVLSLRQAIEVVEQGPEFYDLAAEDMRSA
IRALELLVGRINVENLLDEIFSSFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=476, Percent_Identity=40.1260504201681, Blast_Score=254, Evalue=2e-67,
Organism=Homo sapiens, GI193082993, Length=461, Percent_Identity=40.5639913232104, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=38.4769539078156, Blast_Score=241, Evalue=1e-63,
Organism=Escherichia coli, GI2367268, Length=455, Percent_Identity=34.7252747252747, Blast_Score=234, Evalue=8e-63,
Organism=Caenorhabditis elegans, GI17507259, Length=448, Percent_Identity=33.0357142857143, Blast_Score=191, Evalue=9e-49,
Organism=Saccharomyces cerevisiae, GI6323665, Length=498, Percent_Identity=30.9236947791165, Blast_Score=213, Evalue=5e-56,
Organism=Drosophila melanogaster, GI45550813, Length=313, Percent_Identity=42.8115015974441, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_SILST (Q1GCM0)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614858.1
- ProteinModelPortal:   Q1GCM0
- SMR:   Q1GCM0
- STRING:   Q1GCM0
- GeneID:   4076398
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2864
- NMPDR:   fig|292414.1.peg.1767
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- PhylomeDB:   Q1GCM0
- ProtClustDB:   PRK05291
- BioCyc:   SSP292414:TM1040_2864-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 46401; Mature: 46401

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: BINDING 20-20 BINDING 77-77 BINDING 117-117 BINDING 428-428

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIFALATAQGKAGVAVIRVSGPHAIEIGEKLTRRTLPARGMIFSKLKDSAGEILDEAL
CCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VLSFTSPDSFTGENIVEFQLHGSIAVVSAVMSAIEETGVARLADPGEFTRRALDNGKLDL
EEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCCEEH
TQVEGLADLIDAETEAQRKQAQVILAGTLGDLADRWRTDLIRAASLIEVTIDFADEEVPV
HHHHHHHHHHCCHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
DVTPEVKQLLSGVLHDIEKEVDGVAIAERIREGFEVAIVGSPNVGKSTLLNALAGRTAAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCHHHH
TSEYAGTTRDVIEVRMDLAGLPVTLLDTAGLRETDDHVEGIGIRLAQERAERADIRVFLA
HHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
EEDESFSIQMREGDLRLLPKADERRSAKGAISGRTGQGVDDLVSRISDTLRNRTAHDGIA
ECCCCEEEEEECCCEEECCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
TRARHRETMNRAVLSLRQAIEVVEQGPEFYDLAAEDMRSAIRALELLVGRINVENLLDEI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FSSFCLGK
HHHHCCCC
>Mature Secondary Structure
METIFALATAQGKAGVAVIRVSGPHAIEIGEKLTRRTLPARGMIFSKLKDSAGEILDEAL
CCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
VLSFTSPDSFTGENIVEFQLHGSIAVVSAVMSAIEETGVARLADPGEFTRRALDNGKLDL
EEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCCEEH
TQVEGLADLIDAETEAQRKQAQVILAGTLGDLADRWRTDLIRAASLIEVTIDFADEEVPV
HHHHHHHHHHCCHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCC
DVTPEVKQLLSGVLHDIEKEVDGVAIAERIREGFEVAIVGSPNVGKSTLLNALAGRTAAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHCCHHHH
TSEYAGTTRDVIEVRMDLAGLPVTLLDTAGLRETDDHVEGIGIRLAQERAERADIRVFLA
HHHHCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEE
EEDESFSIQMREGDLRLLPKADERRSAKGAISGRTGQGVDDLVSRISDTLRNRTAHDGIA
ECCCCEEEEEECCCEEECCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHH
TRARHRETMNRAVLSLRQAIEVVEQGPEFYDLAAEDMRSAIRALELLVGRINVENLLDEI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FSSFCLGK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA