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The map label for this gene is rho [H]

Identifier: 99082703

GI number: 99082703

Start: 3031417

End: 3032688

Strand: Direct

Name: rho [H]

Synonym: TM1040_2863

Alternate gene names: 99082703

Gene position: 3031417-3032688 (Clockwise)

Preceding gene: 99082702

Following gene: 99082704

Centisome position: 94.7

GC content: 54.4

Gene sequence:

>1272_bases
ATGACCGAGCAATCTCTGAACCTTTCTGATCTCAAGGCGCAAAGCCCGAAGGCCCTGTTGGCTATGGCTGAGGAGCTGGA
GATCGAAAACGCCTCCACTATGCGGAAAGGCGAGATGATGTTCCAAATCCTGCGTGAACGCGCGGATGAAGGCTGGAACA
TCGGAGGGGACGGGGTTCTCGAGGTCCTGCAGGACGGATTTGGCTTCTTGCGCTCGCCCGAAGCAAACTATCTGCCGGGT
CCAGACGATATCTATGTCTCTCCTGACATGATCCGCCAGTTTTCGCTACGCACTGGGGATACGGTAGAGGGGCAGATCAA
GGCACCACTGGAAAATGAGCGCTACTTTGCGCTGACCACGGTGACAAAGATCAACTTTGAAGAGCCCGAAAAGGCCCGTC
ACAAGATTGCATTCGACAATCTGACACCGCTCTATCCGGACGAGCGTCTGCAGATGGAAATCGAGGATCCGACGGTTAAG
GATCGCTCAGCACGCGTGATTGACCTTGTCTCGCCGATCGGCAAGGGCCAGCGTTCGCTTATTGTAGCGCCGCCGCGAAC
CGGTAAGACAGTTCTACTGCAAAACATCGCCCATTCGATCGAGCAGAACCACCCTGAGTGCTATCTGATCGTGCTTCTGA
TCGACGAGCGCCCTGAAGAAGTCACCGACATGCAGCGCTCCGTCAAAGGCGAGGTCGTGTCCTCCACGTTTGACGAACCC
GCAGCGCGCCACGTGGCGGTTTCCGAGATGGTCATTGAAAAAGCCAAGCGTCTGGTCGAACATAAACGTGACGTTGTGAT
CTTGCTCGACTCGATCACGCGTCTCGGCCGTGCTTTCAACACGGTTGTTCCATCGTCAGGCAAGGTTCTGACTGGTGGTG
TGGATGCAAACGCTCTGCAACGTCCCAAGCGCTTCTTTGGGGCTGCGCGGAATATCGAAGAAGGTGGCTCTTTGACGATC
ATCGCGACCGCGCTTATCGACACGGGCAGCCGTATGGACGAGGTTATCTTTGAAGAATTCAAGGGTACGGGTAACTCTGA
GATCGTTCTGGATCGCAAGATTGCAGACAAGAGGGTCTTCCCTGCGATCGACATTCTCAAGTCGGGAACGCGGAAGGAAG
ACCTCCTGGTCGACAAAGGCGATCTGGCAAAAACCTTTGTGTTGCGTCGCATCCTGAATCCGATGGGGACCACCGACGCA
ATTGAGTTCCTGCTGTCTAAGTTGAAGCAAACAAAGACAAACTCAGAGTTTTTTGACTCGATGAACACCTAA

Upstream 100 bases:

>100_bases
CTATCTAGCGAGAAAGCGGCCCCGACCGGGGATTCCCGCTATTTCCGTATTTTATACTGTGATCCCCTCTCAACGCAGAG
GGAACAAGGGCCGTGTGCAT

Downstream 100 bases:

>100_bases
ACGGGGTAGGGCGGAGAAAGAGGCCATCCCATGGAGACGATCTTTGCGTTGGCCACCGCCCAAGGAAAGGCCGGTGTCGC
CGTTATACGTGTATCCGGTC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTEQSLNLSDLKAQSPKALLAMAEELEIENASTMRKGEMMFQILRERADEGWNIGGDGVLEVLQDGFGFLRSPEANYLPG
PDDIYVSPDMIRQFSLRTGDTVEGQIKAPLENERYFALTTVTKINFEEPEKARHKIAFDNLTPLYPDERLQMEIEDPTVK
DRSARVIDLVSPIGKGQRSLIVAPPRTGKTVLLQNIAHSIEQNHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEP
AARHVAVSEMVIEKAKRLVEHKRDVVILLDSITRLGRAFNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTI
IATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKIADKRVFPAIDILKSGTRKEDLLVDKGDLAKTFVLRRILNPMGTTDA
IEFLLSKLKQTKTNSEFFDSMNT

Sequences:

>Translated_423_residues
MTEQSLNLSDLKAQSPKALLAMAEELEIENASTMRKGEMMFQILRERADEGWNIGGDGVLEVLQDGFGFLRSPEANYLPG
PDDIYVSPDMIRQFSLRTGDTVEGQIKAPLENERYFALTTVTKINFEEPEKARHKIAFDNLTPLYPDERLQMEIEDPTVK
DRSARVIDLVSPIGKGQRSLIVAPPRTGKTVLLQNIAHSIEQNHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEP
AARHVAVSEMVIEKAKRLVEHKRDVVILLDSITRLGRAFNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTI
IATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKIADKRVFPAIDILKSGTRKEDLLVDKGDLAKTFVLRRILNPMGTTDA
IEFLLSKLKQTKTNSEFFDSMNT
>Mature_422_residues
TEQSLNLSDLKAQSPKALLAMAEELEIENASTMRKGEMMFQILRERADEGWNIGGDGVLEVLQDGFGFLRSPEANYLPGP
DDIYVSPDMIRQFSLRTGDTVEGQIKAPLENERYFALTTVTKINFEEPEKARHKIAFDNLTPLYPDERLQMEIEDPTVKD
RSARVIDLVSPIGKGQRSLIVAPPRTGKTVLLQNIAHSIEQNHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEPA
ARHVAVSEMVIEKAKRLVEHKRDVVILLDSITRLGRAFNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTII
ATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKIADKRVFPAIDILKSGTRKEDLLVDKGDLAKTFVLRRILNPMGTTDAI
EFLLSKLKQTKTNSEFFDSMNT

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=416, Percent_Identity=67.5480769230769, Blast_Score=590, Evalue=1e-170,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47203; Mature: 47071

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQSLNLSDLKAQSPKALLAMAEELEIENASTMRKGEMMFQILRERADEGWNIGGDGVL
CCCCCCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
EVLQDGFGFLRSPEANYLPGPDDIYVSPDMIRQFSLRTGDTVEGQIKAPLENERYFALTT
HHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCEEECCCCCCCEEEEEE
VTKINFEEPEKARHKIAFDNLTPLYPDERLQMEIEDPTVKDRSARVIDLVSPIGKGQRSL
EEEECCCCCHHHHHHEECCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCEE
IVAPPRTGKTVLLQNIAHSIEQNHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEP
EEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCCH
AARHVAVSEMVIEKAKRLVEHKRDVVILLDSITRLGRAFNTVVPSSGKVLTGGVDANALQ
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHH
RPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKIADKRVF
HHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHCCCCC
PAIDILKSGTRKEDLLVDKGDLAKTFVLRRILNPMGTTDAIEFLLSKLKQTKTNSEFFDS
HHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHC
MNT
CCC
>Mature Secondary Structure 
TEQSLNLSDLKAQSPKALLAMAEELEIENASTMRKGEMMFQILRERADEGWNIGGDGVL
CCCCCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
EVLQDGFGFLRSPEANYLPGPDDIYVSPDMIRQFSLRTGDTVEGQIKAPLENERYFALTT
HHHHHHHHHHCCCCCCCCCCCCCEEECHHHHHHHHCCCCCCCCCEEECCCCCCCEEEEEE
VTKINFEEPEKARHKIAFDNLTPLYPDERLQMEIEDPTVKDRSARVIDLVSPIGKGQRSL
EEEECCCCCHHHHHHEECCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCCCCEE
IVAPPRTGKTVLLQNIAHSIEQNHPECYLIVLLIDERPEEVTDMQRSVKGEVVSSTFDEP
EEECCCCCCHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCCH
AARHVAVSEMVIEKAKRLVEHKRDVVILLDSITRLGRAFNTVVPSSGKVLTGGVDANALQ
HHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCCHHHH
RPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKIADKRVF
HHHHHHHHHCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHCCCCC
PAIDILKSGTRKEDLLVDKGDLAKTFVLRRILNPMGTTDAIEFLLSKLKQTKTNSEFFDS
HHHHHHHCCCCCCCCEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHC
MNT
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606169 [H]