Click here to switch to the map view.

The map label for this gene is fgs [H]

Identifier: 99082515

GI number: 99082515

Start: 2810634

End: 2811905

Strand: Reverse

Name: fgs [H]

Synonym: TM1040_2675

Alternate gene names: 99082515

Gene position: 2811905-2810634 (Counterclockwise)

Preceding gene: 99082516

Following gene: 99082512

Centisome position: 87.85

GC content: 65.88

Gene sequence:

>1272_bases
ATGACCGAACAGACCTCCGATGCCATTCTCGCCCGCATGATGGCGCTGCACCCCAAGATCATCGACCTGACGCTGGATCG
GGTCTGGCGTCTTCTGGCGGCGCTTGAGAACCCGCAGGACAAGCTGCCCCCGGTGATCCATATCGCGGGCACCAACGGCA
AAGGCTCGACGCAGGCGATGATCCGCGCCGGGCTTGAGGGCTGGGGCAAATCGGTGCACGCCTATACCTCACCGCATCTG
GCGCGCTTTCATGAACGCATCCGTCTGGCGGGCGATCTGATCTCAGAGGCGCATCTCACGGAGGTGCTGGACGAGTGCTA
CAGCGCCAATGGCGACGCAAGCATCACCTATTTCGAGATCACCACCGTCGCGGGGCTTCTGGCGTTTTCACGCACACCCG
CCGACTACACCCTCCTCGAGGTTGGCCTTGGCGGGCGTCTGGATGCCACCAATGTCATCACGCCCGAGGTCTCTGTAATC
ACGCCGGTCTCGATCGATCACGAGCAATTCCTCGGCAATACGCTGGCCAAGATCGCAGGCGAGAAGGCCGGGATCATCAA
ACGCGGCGTGCCCGTGGTGGTCGGCCCCCAGGCCGAAGAGGCGATGGAGGTCATCGAGGACACCGCGATGCGCCTGGGCG
CCCCCCTGATCGCCTATGGCCAGCACTGGCACGTCCACGAGGAACGCGGCCGTCTCGTCTATCAGGACGAGCGCGGCCTG
CTGGACCTGCCGCTGCCCAACCTGATGGGCGCGCATCAGATCCAGAACGCCGGCGCCGCCCTTGCGGTGCTGCGCCACCT
TGGCGCGGATGAGGCCGCCTGCGAGGCGGCTGTTGTGGGGGCACAGTGGCCCGCCCGTATGCAGCGCCTCAAAACCGGCC
CGCTGGTGGAAATCGCAGGCGCGGCCGAGCTGTGGCTCGATGGCGGCCACAACGCCGCTGCCGGGATTGCTCTCGCCGAT
GTGCTGGCCAAGCTGCCCAAACGCCCGACGCATCTGATTTGCGGTATGCTCAATACCAAGGATGTCACAGGCTACCTGCG
CCCGCTCGCCGCCGAGGCCCAGAGCCTCACGGCGGTGTCGATCCCGGGCGAGGCGGCAACCCTCTCGGCCGAGGAAACCA
AAGCGGCGGCCAGTTCCGTGGACCTTCCAGCAACCACTGCGGAGTCTGTTGCCGAGGCGCTCACCGCCATTCTGGCGCGC
GACCCAGACAGCCGCGTGCTGATCTGCGGCTCGCTCTATCTGGCGGGCAATATCCTGCGCGAGAACGGCTAG

Upstream 100 bases:

>100_bases
CCAAACGGGGCGGGCGCTTCGCTTTCACCCCGACGGGACGGGCGCGGCCCTATCGCATGTGGCCCCGTGTCGCTCCGTGA
TCAGACAGGTTCAGGCCGCC

Downstream 100 bases:

>100_bases
GGCGCTTCCGATATCAGCACCAAGGCCCCCGCGCAGAGCCCAGCAGCGAGGGCTTTTGGGGGCGGCGCATTTGTGACGAC
ATAATCGGGATCGTGGAGCC

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTEQTSDAILARMMALHPKIIDLTLDRVWRLLAALENPQDKLPPVIHIAGTNGKGSTQAMIRAGLEGWGKSVHAYTSPHL
ARFHERIRLAGDLISEAHLTEVLDECYSANGDASITYFEITTVAGLLAFSRTPADYTLLEVGLGGRLDATNVITPEVSVI
TPVSIDHEQFLGNTLAKIAGEKAGIIKRGVPVVVGPQAEEAMEVIEDTAMRLGAPLIAYGQHWHVHEERGRLVYQDERGL
LDLPLPNLMGAHQIQNAGAALAVLRHLGADEAACEAAVVGAQWPARMQRLKTGPLVEIAGAAELWLDGGHNAAAGIALAD
VLAKLPKRPTHLICGMLNTKDVTGYLRPLAAEAQSLTAVSIPGEAATLSAEETKAAASSVDLPATTAESVAEALTAILAR
DPDSRVLICGSLYLAGNILRENG

Sequences:

>Translated_423_residues
MTEQTSDAILARMMALHPKIIDLTLDRVWRLLAALENPQDKLPPVIHIAGTNGKGSTQAMIRAGLEGWGKSVHAYTSPHL
ARFHERIRLAGDLISEAHLTEVLDECYSANGDASITYFEITTVAGLLAFSRTPADYTLLEVGLGGRLDATNVITPEVSVI
TPVSIDHEQFLGNTLAKIAGEKAGIIKRGVPVVVGPQAEEAMEVIEDTAMRLGAPLIAYGQHWHVHEERGRLVYQDERGL
LDLPLPNLMGAHQIQNAGAALAVLRHLGADEAACEAAVVGAQWPARMQRLKTGPLVEIAGAAELWLDGGHNAAAGIALAD
VLAKLPKRPTHLICGMLNTKDVTGYLRPLAAEAQSLTAVSIPGEAATLSAEETKAAASSVDLPATTAESVAEALTAILAR
DPDSRVLICGSLYLAGNILRENG
>Mature_422_residues
TEQTSDAILARMMALHPKIIDLTLDRVWRLLAALENPQDKLPPVIHIAGTNGKGSTQAMIRAGLEGWGKSVHAYTSPHLA
RFHERIRLAGDLISEAHLTEVLDECYSANGDASITYFEITTVAGLLAFSRTPADYTLLEVGLGGRLDATNVITPEVSVIT
PVSIDHEQFLGNTLAKIAGEKAGIIKRGVPVVVGPQAEEAMEVIEDTAMRLGAPLIAYGQHWHVHEERGRLVYQDERGLL
DLPLPNLMGAHQIQNAGAALAVLRHLGADEAACEAAVVGAQWPARMQRLKTGPLVEIAGAAELWLDGGHNAAAGIALADV
LAKLPKRPTHLICGMLNTKDVTGYLRPLAAEAQSLTAVSIPGEAATLSAEETKAAASSVDLPATTAESVAEALTAILARD
PDSRVLICGSLYLAGNILRENG

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=309, Percent_Identity=30.7443365695793, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI66932990, Length=309, Percent_Identity=30.7443365695793, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1788654, Length=415, Percent_Identity=33.4939759036145, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI71984923, Length=477, Percent_Identity=26.8343815513627, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17553148, Length=477, Percent_Identity=26.8343815513627, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17553150, Length=477, Percent_Identity=26.8343815513627, Blast_Score=132, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6323760, Length=423, Percent_Identity=30.2600472813239, Blast_Score=163, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6324815, Length=335, Percent_Identity=28.3582089552239, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322718, Length=284, Percent_Identity=26.7605633802817, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24641571, Length=425, Percent_Identity=26.5882352941176, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24581568, Length=176, Percent_Identity=34.0909090909091, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 44855; Mature: 44724

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQTSDAILARMMALHPKIIDLTLDRVWRLLAALENPQDKLPPVIHIAGTNGKGSTQAM
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCHHHHH
IRAGLEGWGKSVHAYTSPHLARFHERIRLAGDLISEAHLTEVLDECYSANGDASITYFEI
HHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEH
TTVAGLLAFSRTPADYTLLEVGLGGRLDATNVITPEVSVITPVSIDHEQFLGNTLAKIAG
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCC
EKAGIIKRGVPVVVGPQAEEAMEVIEDTAMRLGAPLIAYGQHWHVHEERGRLVYQDERGL
CCCCHHHCCCCEEECCCHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCEEEECCCCE
LDLPLPNLMGAHQIQNAGAALAVLRHLGADEAACEAAVVGAQWPARMQRLKTGPLVEIAG
EECCCCHHHCHHHHHHCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHCCCCCCEEECC
AAELWLDGGHNAAAGIALADVLAKLPKRPTHLICGMLNTKDVTGYLRPLAAEAQSLTAVS
CCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHEECCCCCHHHHHHHHHHHHHHCEEEEE
IPGEAATLSAEETKAAASSVDLPATTAESVAEALTAILARDPDSRVLICGSLYLAGNILR
CCCCCEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHH
ENG
CCC
>Mature Secondary Structure 
TEQTSDAILARMMALHPKIIDLTLDRVWRLLAALENPQDKLPPVIHIAGTNGKGSTQAM
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCHHHHH
IRAGLEGWGKSVHAYTSPHLARFHERIRLAGDLISEAHLTEVLDECYSANGDASITYFEI
HHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEH
TTVAGLLAFSRTPADYTLLEVGLGGRLDATNVITPEVSVITPVSIDHEQFLGNTLAKIAG
HHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCC
EKAGIIKRGVPVVVGPQAEEAMEVIEDTAMRLGAPLIAYGQHWHVHEERGRLVYQDERGL
CCCCHHHCCCCEEECCCHHHHHHHHHHHHHHHCCCCEECCCEEEEECCCCCEEEECCCCE
LDLPLPNLMGAHQIQNAGAALAVLRHLGADEAACEAAVVGAQWPARMQRLKTGPLVEIAG
EECCCCHHHCHHHHHHCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHCCCCCCEEECC
AAELWLDGGHNAAAGIALADVLAKLPKRPTHLICGMLNTKDVTGYLRPLAAEAQSLTAVS
CCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHEECCCCCHHHHHHHHHHHHHHCEEEEE
IPGEAATLSAEETKAAASSVDLPATTAESVAEALTAILARDPDSRVLICGSLYLAGNILR
CCCCCEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHH
ENG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2105929; 9618466 [H]