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The map label for this gene is mepA [H]

Identifier: 99082503

GI number: 99082503

Start: 2798512

End: 2799435

Strand: Direct

Name: mepA [H]

Synonym: TM1040_2663

Alternate gene names: 99082503

Gene position: 2798512-2799435 (Clockwise)

Preceding gene: 99082502

Following gene: 99082504

Centisome position: 87.43

GC content: 65.26

Gene sequence:

>924_bases
TTGAGGTTATTGCGACATATCACCCTCGCGGCCTTTGTGCTTGCGGCCTGCGCCCCGGCGCAAGACAGCCCACGCCCCTC
TGCCGCCGCCTTCGCCAACAAGGCCGCCAGTTCCACCGTGCCGGCCAAACAGCTGTTCGGCGCAAAACGCGGCCCCTCCG
CCCAGCGCCCCGCGCCACTGGGATCTTATGCCAAAGGCTGTGTCGCAGGCGCGGCACAACTGCCGGAAACCGGCCCCACC
TGGCAGGCCATGCGCCTCAGTCGCAATCGCAACTGGGGCCACCCCGAAACCATTGATTTCATTCAGGACCTGAGCCGTGT
TGCGGCACGACAGCCGGGCTGGCAGGGGCTCTATGTGGGCGACATCAGCCAGCCGCGGGGCGGGCCGATGCTGTCGGGCC
ATCGCAGCCATCAGATCGGCCTTGATGCCGACATCTGGATGCTGCCCCCCGACCGGCTCGACCTCAGCCGCGAGGCACGC
GAGAATATCTCGTCGATCTCGTTGCGCCGCGCCAGTGGTGCCTATGTGAACGACAACTGGAGCGCCCAGCATCACGCGAT
CCTGAAGGCCGCCGCCTCCGACCCCCGAGTGGCGCGGATCTTTGTCTTTCCCGGCGCCAAGGTCAAAATGTGCGACACCG
AGACCGGCGACCGAAGCTATCTGCGCAAGATCCGGCCGTGGTGGGGGCATCACTACCACTTCCATGTGCGTCTGAATTGT
CCTCGTGGCACCCGTGGCTGTGTCGAGCAGGATCCGCCGCCACGCGGGGATGGCTGCGATGATGCGCGCCAGTGGGTCGC
CAATATCCTGAACCCGCCGCCCCCAAAACCGGTTGACCCGAACGCGCCAAAGCCTAAACCGCGCAGGGATTACACCCTTT
CGGATCTGCCTCAGCAATGCGCCGCCGTTCTTTCATCCGACTAG

Upstream 100 bases:

>100_bases
CACTGACGGGCAGTGCACGCCTTGGCATTACACCAGTGATTGCGCTCTTTGCCCTCGGTCTGCTTTTATTGCTTTGGGTG
GATGGTGAAGGAGATCAGTC

Downstream 100 bases:

>100_bases
CCTCCGTCGCGATCGGCCTCTTGGTCGCTCTCTGCGCGGCTGCCCTTGCGGCCTCCACCGTCTGGAGCGTGACTCACGCC
GACAGCAACAGCGCCCGGCT

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRLLRHITLAAFVLAACAPAQDSPRPSAAAFANKAASSTVPAKQLFGAKRGPSAQRPAPLGSYAKGCVAGAAQLPETGPT
WQAMRLSRNRNWGHPETIDFIQDLSRVAARQPGWQGLYVGDISQPRGGPMLSGHRSHQIGLDADIWMLPPDRLDLSREAR
ENISSISLRRASGAYVNDNWSAQHHAILKAAASDPRVARIFVFPGAKVKMCDTETGDRSYLRKIRPWWGHHYHFHVRLNC
PRGTRGCVEQDPPPRGDGCDDARQWVANILNPPPPKPVDPNAPKPKPRRDYTLSDLPQQCAAVLSSD

Sequences:

>Translated_307_residues
MRLLRHITLAAFVLAACAPAQDSPRPSAAAFANKAASSTVPAKQLFGAKRGPSAQRPAPLGSYAKGCVAGAAQLPETGPT
WQAMRLSRNRNWGHPETIDFIQDLSRVAARQPGWQGLYVGDISQPRGGPMLSGHRSHQIGLDADIWMLPPDRLDLSREAR
ENISSISLRRASGAYVNDNWSAQHHAILKAAASDPRVARIFVFPGAKVKMCDTETGDRSYLRKIRPWWGHHYHFHVRLNC
PRGTRGCVEQDPPPRGDGCDDARQWVANILNPPPPKPVDPNAPKPKPRRDYTLSDLPQQCAAVLSSD
>Mature_307_residues
MRLLRHITLAAFVLAACAPAQDSPRPSAAAFANKAASSTVPAKQLFGAKRGPSAQRPAPLGSYAKGCVAGAAQLPETGPT
WQAMRLSRNRNWGHPETIDFIQDLSRVAARQPGWQGLYVGDISQPRGGPMLSGHRSHQIGLDADIWMLPPDRLDLSREAR
ENISSISLRRASGAYVNDNWSAQHHAILKAAASDPRVARIFVFPGAKVKMCDTETGDRSYLRKIRPWWGHHYHFHVRLNC
PRGTRGCVEQDPPPRGDGCDDARQWVANILNPPPPKPVDPNAPKPKPRRDYTLSDLPQQCAAVLSSD

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=246, Percent_Identity=36.9918699186992, Blast_Score=154, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 33654; Mature: 33654

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLRHITLAAFVLAACAPAQDSPRPSAAAFANKAASSTVPAKQLFGAKRGPSAQRPAPL
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCC
GSYAKGCVAGAAQLPETGPTWQAMRLSRNRNWGHPETIDFIQDLSRVAARQPGWQGLYVG
CHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
DISQPRGGPMLSGHRSHQIGLDADIWMLPPDRLDLSREARENISSISLRRASGAYVNDNW
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCC
SAQHHAILKAAASDPRVARIFVFPGAKVKMCDTETGDRSYLRKIRPWWGHHYHFHVRLNC
CHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEEC
PRGTRGCVEQDPPPRGDGCDDARQWVANILNPPPPKPVDPNAPKPKPRRDYTLSDLPQQC
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AAVLSSD
HHHHCCC
>Mature Secondary Structure
MRLLRHITLAAFVLAACAPAQDSPRPSAAAFANKAASSTVPAKQLFGAKRGPSAQRPAPL
CHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCC
GSYAKGCVAGAAQLPETGPTWQAMRLSRNRNWGHPETIDFIQDLSRVAARQPGWQGLYVG
CHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEE
DISQPRGGPMLSGHRSHQIGLDADIWMLPPDRLDLSREARENISSISLRRASGAYVNDNW
CCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEECCCC
SAQHHAILKAAASDPRVARIFVFPGAKVKMCDTETGDRSYLRKIRPWWGHHYHFHVRLNC
CHHHHHHHHHHCCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHHHHCCCEEEEEEEEC
PRGTRGCVEQDPPPRGDGCDDARQWVANILNPPPPKPVDPNAPKPKPRRDYTLSDLPQQC
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AAVLSSD
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]