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The map label for this gene is waaA [H]

Identifier: 99082481

GI number: 99082481

Start: 2775250

End: 2776446

Strand: Direct

Name: waaA [H]

Synonym: TM1040_2641

Alternate gene names: 99082481

Gene position: 2775250-2776446 (Clockwise)

Preceding gene: 99082480

Following gene: 99082482

Centisome position: 86.7

GC content: 65.16

Gene sequence:

>1197_bases
ATGTTTGGCCCCGGCGGAACACAGATCCCAAACCCCCTGCGGCTGAAACGCCCCGAGGGCGAGCTGGTCTGGGCCCATGC
GACCACCCAGGAACGTCTCTTGGGGCTTTGTGACGTGGGCTGTCGTCTCAAAATGATGCGCCCGGACCTCTCGGTGATGC
TCACGTGGGAAGAGGACATGCGGCCCGCCAAATTGCCCGAGGGCTGCGACATCCCGCTCGGGCCGCTGACGGTGGAGCAA
CCCAACGACATTCGCAACTTTCTGGACAATTGGTCTCCCGATGTCTGCGTCTGGGCGGGCGGGCGTTTGCGTCGCCTCCT
GATGCGCCATATGCGCGAGCGGGAGATGCCCGCGCTCTTGTGCGACATCGACGCGGACGAGCTGCCAAGCCGGGCTTCGC
GCTGGCTGCCGGATCAGCGCCATCGTCTGCTCAACGGGTTTGCCGCGATCCTGGTGCCAGGCACCGAGGTCTCAGAGCGG
CTGAAACGTGCAGGTGTTGCGCCCGAACGCATCCACCCCGCAGGCCGCCTGTTTCAATCCAGCACCCCGCCAAGCTGCAA
CGACGACGAACTGGCGCAGATGCAGAAACAATTTGCCAGCCGCCCGCTCTGGCTTGCCGCGCATGTCTCGCTTTCGGAAC
TCCCCGCCGTGCTCAAGGCGCACCGGGGGGCCCTGCGACTGCTGCACCGGCTCTTGCTGGTGCTCACGGTGGACACGTTT
GAGGATCTCGACGCCGCCCGCAGCCTCCTCAGAAAAGAGGGGCTCTCCTTTGCCGACTGGGACATGGGCGAAGACCCCGA
GGACCACACGCAGGTCGCCATTGGCCTCACGGAAAATCTTGGTCTGTGGTATCGGCTCTGCCCGATCAGTTTCCTCGGCA
ACAGCCTCATTCGGGGGGCGCAGGGCACCAACCCGCTGGACGCCGCCGCACTTGGCTCTGCGATCCTGCATGGCCCCGGC
GTCGTCGCCCATGCCCAGGCCTATCAGCGCCTCGCGGCCCTTGATGCGGCCGAGCGGATCCACGGCGAAGAAGAGCTTGC
CGATGCTGTCTTTCGCCTGTCATCACCGGACCGTGCCGCCGAAATGGCCCTTGCGGGCTGGCAGGTGGTGACCGAAGGTG
CGGTCATGACAGACACCCTCCTAGAGCGGATCCAGGATCTGCTGGATCAAAGCGAACTCTCCCATGCGCCCGCCTGA

Upstream 100 bases:

>100_bases
GCGCCGACGCGCTGCAAAACGGGGTTGTCGCGGGCGGACCCAAGATGCACCGCGCCTTGCTCAACCGGCTGGTCTATGGC
GCACAGGGGTCCAAACCCTG

Downstream 100 bases:

>100_bases
ATTCTGGAACAAACCGCCCAGCCACTTTGACCTGCGCGCCTGCCTGCTGTCGCCGCTGGGCGCTCTTTATGCCAAAGGCA
CTGCCACGCGGCTGCGCAAG

Product: 3-deoxy-D-manno-octulosonic-acid transferase, putative

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MFGPGGTQIPNPLRLKRPEGELVWAHATTQERLLGLCDVGCRLKMMRPDLSVMLTWEEDMRPAKLPEGCDIPLGPLTVEQ
PNDIRNFLDNWSPDVCVWAGGRLRRLLMRHMREREMPALLCDIDADELPSRASRWLPDQRHRLLNGFAAILVPGTEVSER
LKRAGVAPERIHPAGRLFQSSTPPSCNDDELAQMQKQFASRPLWLAAHVSLSELPAVLKAHRGALRLLHRLLLVLTVDTF
EDLDAARSLLRKEGLSFADWDMGEDPEDHTQVAIGLTENLGLWYRLCPISFLGNSLIRGAQGTNPLDAAALGSAILHGPG
VVAHAQAYQRLAALDAAERIHGEEELADAVFRLSSPDRAAEMALAGWQVVTEGAVMTDTLLERIQDLLDQSELSHAPA

Sequences:

>Translated_398_residues
MFGPGGTQIPNPLRLKRPEGELVWAHATTQERLLGLCDVGCRLKMMRPDLSVMLTWEEDMRPAKLPEGCDIPLGPLTVEQ
PNDIRNFLDNWSPDVCVWAGGRLRRLLMRHMREREMPALLCDIDADELPSRASRWLPDQRHRLLNGFAAILVPGTEVSER
LKRAGVAPERIHPAGRLFQSSTPPSCNDDELAQMQKQFASRPLWLAAHVSLSELPAVLKAHRGALRLLHRLLLVLTVDTF
EDLDAARSLLRKEGLSFADWDMGEDPEDHTQVAIGLTENLGLWYRLCPISFLGNSLIRGAQGTNPLDAAALGSAILHGPG
VVAHAQAYQRLAALDAAERIHGEEELADAVFRLSSPDRAAEMALAGWQVVTEGAVMTDTLLERIQDLLDQSELSHAPA
>Mature_398_residues
MFGPGGTQIPNPLRLKRPEGELVWAHATTQERLLGLCDVGCRLKMMRPDLSVMLTWEEDMRPAKLPEGCDIPLGPLTVEQ
PNDIRNFLDNWSPDVCVWAGGRLRRLLMRHMREREMPALLCDIDADELPSRASRWLPDQRHRLLNGFAAILVPGTEVSER
LKRAGVAPERIHPAGRLFQSSTPPSCNDDELAQMQKQFASRPLWLAAHVSLSELPAVLKAHRGALRLLHRLLLVLTVDTF
EDLDAARSLLRKEGLSFADWDMGEDPEDHTQVAIGLTENLGLWYRLCPISFLGNSLIRGAQGTNPLDAAALGSAILHGPG
VVAHAQAYQRLAALDAAERIHGEEELADAVFRLSSPDRAAEMALAGWQVVTEGAVMTDTLLERIQDLLDQSELSHAPA

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=282, Percent_Identity=26.9503546099291, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 44015; Mature: 44015

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGPGGTQIPNPLRLKRPEGELVWAHATTQERLLGLCDVGCRLKMMRPDLSVMLTWEEDM
CCCCCCCCCCCCCEEECCCCCEEEEECCHHHHHHHHHCCCCEEEEECCCCEEEEEEHHCC
RPAKLPEGCDIPLGPLTVEQPNDIRNFLDNWSPDVCVWAGGRLRRLLMRHMREREMPALL
CCCCCCCCCCCCCCCEECCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCCEEE
CDIDADELPSRASRWLPDQRHRLLNGFAAILVPGTEVSERLKRAGVAPERIHPAGRLFQS
ECCCHHHCCHHHHHCCCHHHHHHHHHHHEEEECCHHHHHHHHHHCCCHHHCCHHHHHHCC
STPPSCNDDELAQMQKQFASRPLWLAAHVSLSELPAVLKAHRGALRLLHRLLLVLTVDTF
CCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDLDAARSLLRKEGLSFADWDMGEDPEDHTQVAIGLTENLGLWYRLCPISFLGNSLIRGA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
QGTNPLDAAALGSAILHGPGVVAHAQAYQRLAALDAAERIHGEEELADAVFRLSSPDRAA
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHH
EMALAGWQVVTEGAVMTDTLLERIQDLLDQSELSHAPA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFGPGGTQIPNPLRLKRPEGELVWAHATTQERLLGLCDVGCRLKMMRPDLSVMLTWEEDM
CCCCCCCCCCCCCEEECCCCCEEEEECCHHHHHHHHHCCCCEEEEECCCCEEEEEEHHCC
RPAKLPEGCDIPLGPLTVEQPNDIRNFLDNWSPDVCVWAGGRLRRLLMRHMREREMPALL
CCCCCCCCCCCCCCCEECCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCCEEE
CDIDADELPSRASRWLPDQRHRLLNGFAAILVPGTEVSERLKRAGVAPERIHPAGRLFQS
ECCCHHHCCHHHHHCCCHHHHHHHHHHHEEEECCHHHHHHHHHHCCCHHHCCHHHHHHCC
STPPSCNDDELAQMQKQFASRPLWLAAHVSLSELPAVLKAHRGALRLLHRLLLVLTVDTF
CCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EDLDAARSLLRKEGLSFADWDMGEDPEDHTQVAIGLTENLGLWYRLCPISFLGNSLIRGA
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCC
QGTNPLDAAALGSAILHGPGVVAHAQAYQRLAALDAAERIHGEEELADAVFRLSSPDRAA
CCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHH
EMALAGWQVVTEGAVMTDTLLERIQDLLDQSELSHAPA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]