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The map label for this gene is def [H]

Identifier: 99082420

GI number: 99082420

Start: 2717810

End: 2718334

Strand: Reverse

Name: def [H]

Synonym: TM1040_2580

Alternate gene names: 99082420

Gene position: 2718334-2717810 (Counterclockwise)

Preceding gene: 99082422

Following gene: 99082419

Centisome position: 84.92

GC content: 59.81

Gene sequence:

>525_bases
ATGAAACGCTCGATCCTGATCCACCCCGACCCGCGTCTGAAGAAGATCGCCACCGATGTGCCGGACCTTTCCGACGACTT
GCGCGCGCTTGCCGACGACATGCTGGAAACCATGTATGCCGCGCCCGGCATCGGTCTTGCTGCGCCGCAAATCGGCGTTC
TGGATCGGCTGATCGTCATGGACTGCGTCAAGGAAGGCGAGGGTGACGCCCGCCCGCTGGTGATGTTCAATCCGCGGGTC
ATTGCGTCCTCGGATGAAACCAACGTCTACGAGGAAGGCTGCCTCTCCATCCCTGAGCAATATGCCGAAGTGACCCGCCC
CAAAGTGGTGGATGTCGAATGGATGGATCGGGACGGCAACCTGCAGACCGAGACATTCGACGGCCTCTGGGCCACCTGCG
TTCAGCATGAAATCGACCACCTCGACGGTAAACTTTTCATCGATTACCTGAAGCCGCTCAAGCGCCAGATGATCACCCGC
AAGATGCAAAAGCTCAAACGCGAACGCGCGCGCGGCACCGCATGA

Upstream 100 bases:

>100_bases
CCCCGCCAATGTGCTGGTACAAACACGCTCGGCCACTCCCGTAGGCTGCGCCGCGAAGAAGCGTTGCGGCCCTGCGGGGA
TTGGCCTAAAGCCTTTGCCC

Downstream 100 bases:

>100_bases
GCCTGTTGCCTATCGTCCAATGGCCGGATCCGCGCCTGTCGACGGCCTGTGCTCCGGTGGGGGCTGCCGAAGACCTTGGC
ACGCTGATCGACGATGTGTT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MKRSILIHPDPRLKKIATDVPDLSDDLRALADDMLETMYAAPGIGLAAPQIGVLDRLIVMDCVKEGEGDARPLVMFNPRV
IASSDETNVYEEGCLSIPEQYAEVTRPKVVDVEWMDRDGNLQTETFDGLWATCVQHEIDHLDGKLFIDYLKPLKRQMITR
KMQKLKRERARGTA

Sequences:

>Translated_174_residues
MKRSILIHPDPRLKKIATDVPDLSDDLRALADDMLETMYAAPGIGLAAPQIGVLDRLIVMDCVKEGEGDARPLVMFNPRV
IASSDETNVYEEGCLSIPEQYAEVTRPKVVDVEWMDRDGNLQTETFDGLWATCVQHEIDHLDGKLFIDYLKPLKRQMITR
KMQKLKRERARGTA
>Mature_174_residues
MKRSILIHPDPRLKKIATDVPDLSDDLRALADDMLETMYAAPGIGLAAPQIGVLDRLIVMDCVKEGEGDARPLVMFNPRV
IASSDETNVYEEGCLSIPEQYAEVTRPKVVDVEWMDRDGNLQTETFDGLWATCVQHEIDHLDGKLFIDYLKPLKRQMITR
KMQKLKRERARGTA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=48.5207100591716, Blast_Score=154, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=33.1210191082803, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=31.0126582278481, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19710; Mature: 19710

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSILIHPDPRLKKIATDVPDLSDDLRALADDMLETMYAAPGIGLAAPQIGVLDRLIVM
CCCCEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
DCVKEGEGDARPLVMFNPRVIASSDETNVYEEGCLSIPEQYAEVTRPKVVDVEWMDRDGN
HHHHCCCCCCCCEEEECCEEEECCCCCHHHHHHHHCCHHHHHHHCCCEEEEEEEECCCCC
LQTETFDGLWATCVQHEIDHLDGKLFIDYLKPLKRQMITRKMQKLKRERARGTA
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKRSILIHPDPRLKKIATDVPDLSDDLRALADDMLETMYAAPGIGLAAPQIGVLDRLIVM
CCCCEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
DCVKEGEGDARPLVMFNPRVIASSDETNVYEEGCLSIPEQYAEVTRPKVVDVEWMDRDGN
HHHHCCCCCCCCEEEECCEEEECCCCCHHHHHHHHCCHHHHHHHCCCEEEEEEEECCCCC
LQTETFDGLWATCVQHEIDHLDGKLFIDYLKPLKRQMITRKMQKLKRERARGTA
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]