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The map label for this gene is rnhA

Identifier: 99082413

GI number: 99082413

Start: 2713912

End: 2714385

Strand: Direct

Name: rnhA

Synonym: TM1040_2573

Alternate gene names: 99082413

Gene position: 2713912-2714385 (Clockwise)

Preceding gene: 99082412

Following gene: 99082414

Centisome position: 84.78

GC content: 60.13

Gene sequence:

>474_bases
ATGCCTGATTTGTTTGCCTATACCGACGGAGCCTGCTCCGGTAATCCCGGCCCCGGTGGCTGGGGCGCCCTCTTGCGTGC
CATGGACGGGGAGACCGTCCTGAAGGAGCGCGAACTCAAGGGCGGCGAAAAAGAGACGACCAACAACCGGATGGAGCTTC
TGGCCGCGATTCATGCGCTCGAGAGCCTTGCACGCCCCTCAAAGATCACCGTGGTGACGGACTCCGCCTATGTGAAGAAC
GGCGTCACAGGCTGGATCTTTGGCTGGAAAAAGAACGGCTGGAAGACATCGGCGAAAAAGCCCGTCAAGAACGTAGAGCT
TTGGCAACGTCTGGACGCCGCACAATCTCGCCATGACGTCACCTGGGAGTGGGTCAAGGGCCATGCGGGCCATCCGGAAA
ACGAGCGCGCGGACGAGCTGGCACGCGCAGGAATGGCGCCGTTCAAATCCTCTGGAAAATCTTCCAAAGGCTGA

Upstream 100 bases:

>100_bases
GCCCTGCTCGCCGAGGCAGGCTTTGAGGTTCTTGGCGCCCGCTGTGCCAATAGCAAAGGATTGGCTGGCGCCCGCGAGAC
CTTTGTCGTACTGACCGCCC

Downstream 100 bases:

>100_bases
GATCGAGCCCCGCGCAGACATACCTGCGCTGTGCGCCGTGCGTGTCCGGCGCCCTTGGTTATACGCCGCCACCCAGCTAG
GATCGGGGCAACTCTAAGCG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MPDLFAYTDGACSGNPGPGGWGALLRAMDGETVLKERELKGGEKETTNNRMELLAAIHALESLARPSKITVVTDSAYVKN
GVTGWIFGWKKNGWKTSAKKPVKNVELWQRLDAAQSRHDVTWEWVKGHAGHPENERADELARAGMAPFKSSGKSSKG

Sequences:

>Translated_157_residues
MPDLFAYTDGACSGNPGPGGWGALLRAMDGETVLKERELKGGEKETTNNRMELLAAIHALESLARPSKITVVTDSAYVKN
GVTGWIFGWKKNGWKTSAKKPVKNVELWQRLDAAQSRHDVTWEWVKGHAGHPENERADELARAGMAPFKSSGKSSKG
>Mature_156_residues
PDLFAYTDGACSGNPGPGGWGALLRAMDGETVLKERELKGGEKETTNNRMELLAAIHALESLARPSKITVVTDSAYVKNG
VTGWIFGWKKNGWKTSAKKPVKNVELWQRLDAAQSRHDVTWEWVKGHAGHPENERADELARAGMAPFKSSGKSSKG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=150, Percent_Identity=40, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI1786408, Length=142, Percent_Identity=61.2676056338028, Blast_Score=181, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI115532542, Length=148, Percent_Identity=38.5135135135135, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI45552509, Length=154, Percent_Identity=36.3636363636364, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137476, Length=154, Percent_Identity=36.3636363636364, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_SILST (Q1GDG1)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614567.1
- ProteinModelPortal:   Q1GDG1
- SMR:   Q1GDG1
- STRING:   Q1GDG1
- GeneID:   4076704
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2573
- NMPDR:   fig|292414.1.peg.18
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q1GDG1
- ProtClustDB:   PRK00203
- BioCyc:   SSP292414:TM1040_2573-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17133; Mature: 17002

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLFAYTDGACSGNPGPGGWGALLRAMDGETVLKERELKGGEKETTNNRMELLAAIHAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ESLARPSKITVVTDSAYVKNGVTGWIFGWKKNGWKTSAKKPVKNVELWQRLDAAQSRHDV
HHHCCCCEEEEEECCHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
TWEWVKGHAGHPENERADELARAGMAPFKSSGKSSKG
CHHEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PDLFAYTDGACSGNPGPGGWGALLRAMDGETVLKERELKGGEKETTNNRMELLAAIHAL
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
ESLARPSKITVVTDSAYVKNGVTGWIFGWKKNGWKTSAKKPVKNVELWQRLDAAQSRHDV
HHHCCCCEEEEEECCHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
TWEWVKGHAGHPENERADELARAGMAPFKSSGKSSKG
CHHEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA