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The map label for this gene is kefA [C]

Identifier: 99082347

GI number: 99082347

Start: 2647796

End: 2649223

Strand: Reverse

Name: kefA [C]

Synonym: TM1040_2507

Alternate gene names: 99082347

Gene position: 2649223-2647796 (Counterclockwise)

Preceding gene: 99082350

Following gene: 99082346

Centisome position: 82.76

GC content: 58.47

Gene sequence:

>1428_bases
ATGGAGCAAGACATTCTACCCGAAGAGGTACAGGGCACGTTGGATTACCTTTTGGAACTGGCGCAAGACTATTGGGAAAT
CGCGCTCACCCCGGGGTGGCGGCAGTACCAGGTGTTGATCGTGATCGCTCTGGTTCTGGCGTCGATCCTGATGCGAAAGC
TGACCCAAGGGCGCTGGGAAGCTTGGGCGCGGTCGCGTGAGGGCTGGCCAAAGTGGCGCCTTCGCCTGGCCGTGCAAGTG
CTCAGGCGGCTGTCGCTGATCTATTTCACTGTTTTTTCTTGGGTGGTCTTCGCGGTCATGCAAGAGGTGACATGGCCCTC
CCGCAGCTATCTTATCGGGGCGGTTGCAAAACTGGCGACCGCGTGGCTGGTGATTGCGCTGATGGCGCGCTTTATTGCCA
ACCCAACCCTGCGCAAACTCGCCTCTTGGCTCTTGTGGATCTACGCAACGCTTGCAGTCTTCAACCTGGTGGATGATACC
GCGCGGGTTCTCGATTCCACCGCCTTTGATATTGGGTCCTTCCATCTCTCGCTGCTGGTTGTGCTCAAGGCTCTGGTGTT
GGCGACCGCTTTGCTTACATTTGCGCGCATGGGCTCTGCTCTGGCTGAGCGTGGCCTCAGCGGCAACGAGGATCTGTCGC
CCTCCATGAAGGTGCTCGCCGGCAAGGCGGTGCAGGTGCTTTTGTACGGGATCGCATTTGTGATTGTGATCCGCTCCATC
GGGTTTGATCTCACGGGGCTGGCCGTCCTGTCGGGGGCGGTGGGCGTCGGGATTGGTTTTGGCTTGCAAAAAGTCGTCTC
CAACCTGGTCTCAGGCATCATCATCTTGATGGATCGCTCGATTAAGCCGGGAGATGTGATTTCCCTTGGAGAGACCTTTG
GTTGGATCAATGCCCTGGGCGCGCGCTACGTCTCTGTGGTGACGCGGGATGGCAAGGAGTACCTGATCCCGAACGAGGAT
CTGATTACCTCGCAAGTGGTGAACTGGTCGCATTCCAACCGCTATGTGCGGCTCGATATTCACTTTGGCACAAGCTATGA
TGACGATCCCCATTTGGTGCGCAAAGTGGCTATTGCTGCGACGATGACCGTGGACCGCGTGCTGGATCGCGATGGTATGC
GGCCGGTCTGTCATATCGTTGGATTCGGGGACAGCAGCGTCGATTACATCCTGCGCTTCTGGATCGCGGATCCAACGGGC
GGACTTACGAATATTCGCGGCAATGTGTTTCTCGCGCTTTGGGACGCCTTCAAGGCCGAGGGGGTTTCGATCCCCTTCCC
GCAGCGGGAATTGCGCCTGTTAAACAATAGTCTGGCTGTCGAGATGGCCGAAGGGCGTATGGGGCCTAAGGGTGACGTCG
AGGGCGAGGAGCAGGGCAATGCGGAGCGCGCGACACAGTCGCGCGGTGCAGCCCCGAAGCTTGATTGA

Upstream 100 bases:

>100_bases
CAATCAAGATTCGTTCTCCGTGCAGTCCGATCACAAGACAGGCAGTTCCCCTTGCAGAGTGCCCCAACTGCGGCACATGC
TGGTGCGAAGGAGGACAGGA

Downstream 100 bases:

>100_bases
AAAGGCGCGGCGCAGTTGCTACATTTGAGACGACCCCAGCGCCGGGGGTCTGACGGCGCGCAGCAAGGAGGACAATGTCC
TGGCACCCCAACCTCAGGCG

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MEQDILPEEVQGTLDYLLELAQDYWEIALTPGWRQYQVLIVIALVLASILMRKLTQGRWEAWARSREGWPKWRLRLAVQV
LRRLSLIYFTVFSWVVFAVMQEVTWPSRSYLIGAVAKLATAWLVIALMARFIANPTLRKLASWLLWIYATLAVFNLVDDT
ARVLDSTAFDIGSFHLSLLVVLKALVLATALLTFARMGSALAERGLSGNEDLSPSMKVLAGKAVQVLLYGIAFVIVIRSI
GFDLTGLAVLSGAVGVGIGFGLQKVVSNLVSGIIILMDRSIKPGDVISLGETFGWINALGARYVSVVTRDGKEYLIPNED
LITSQVVNWSHSNRYVRLDIHFGTSYDDDPHLVRKVAIAATMTVDRVLDRDGMRPVCHIVGFGDSSVDYILRFWIADPTG
GLTNIRGNVFLALWDAFKAEGVSIPFPQRELRLLNNSLAVEMAEGRMGPKGDVEGEEQGNAERATQSRGAAPKLD

Sequences:

>Translated_475_residues
MEQDILPEEVQGTLDYLLELAQDYWEIALTPGWRQYQVLIVIALVLASILMRKLTQGRWEAWARSREGWPKWRLRLAVQV
LRRLSLIYFTVFSWVVFAVMQEVTWPSRSYLIGAVAKLATAWLVIALMARFIANPTLRKLASWLLWIYATLAVFNLVDDT
ARVLDSTAFDIGSFHLSLLVVLKALVLATALLTFARMGSALAERGLSGNEDLSPSMKVLAGKAVQVLLYGIAFVIVIRSI
GFDLTGLAVLSGAVGVGIGFGLQKVVSNLVSGIIILMDRSIKPGDVISLGETFGWINALGARYVSVVTRDGKEYLIPNED
LITSQVVNWSHSNRYVRLDIHFGTSYDDDPHLVRKVAIAATMTVDRVLDRDGMRPVCHIVGFGDSSVDYILRFWIADPTG
GLTNIRGNVFLALWDAFKAEGVSIPFPQRELRLLNNSLAVEMAEGRMGPKGDVEGEEQGNAERATQSRGAAPKLD
>Mature_475_residues
MEQDILPEEVQGTLDYLLELAQDYWEIALTPGWRQYQVLIVIALVLASILMRKLTQGRWEAWARSREGWPKWRLRLAVQV
LRRLSLIYFTVFSWVVFAVMQEVTWPSRSYLIGAVAKLATAWLVIALMARFIANPTLRKLASWLLWIYATLAVFNLVDDT
ARVLDSTAFDIGSFHLSLLVVLKALVLATALLTFARMGSALAERGLSGNEDLSPSMKVLAGKAVQVLLYGIAFVIVIRSI
GFDLTGLAVLSGAVGVGIGFGLQKVVSNLVSGIIILMDRSIKPGDVISLGETFGWINALGARYVSVVTRDGKEYLIPNED
LITSQVVNWSHSNRYVRLDIHFGTSYDDDPHLVRKVAIAATMTVDRVLDRDGMRPVCHIVGFGDSSVDYILRFWIADPTG
GLTNIRGNVFLALWDAFKAEGVSIPFPQRELRLLNNSLAVEMAEGRMGPKGDVEGEEQGNAERATQSRGAAPKLD

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=226, Percent_Identity=29.646017699115, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI2367355, Length=258, Percent_Identity=27.5193798449612, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1789291, Length=208, Percent_Identity=27.8846153846154, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 52551; Mature: 52551

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure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CCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]