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The map label for this gene is smpB

Identifier: 99082322

GI number: 99082322

Start: 2621561

End: 2622037

Strand: Reverse

Name: smpB

Synonym: TM1040_2482

Alternate gene names: 99082322

Gene position: 2622037-2621561 (Counterclockwise)

Preceding gene: 99082323

Following gene: 99082319

Centisome position: 81.91

GC content: 56.6

Gene sequence:

>477_bases
ATGGCGAAACAAAAGACAGACCCGAATTACAAAGTCATAGCTGAAAACCGGCGCGCGCGGTTTGACTATGCGATCGAGCA
GGATCTCGAATGCGGCATCATGCTTGAAGGGTCCGAGGTGAAATCCCTGCGCGCGGGTGGCTCCAACATCGCGGAGTCCT
ATGCTGCGGTGGAGGAAGGCGAGCTGTGGCTGGTCAATTCCTATATCGCGCCCTACGAGCAGGCGAAGGTTTTCAAGCAC
GAAGAGCGCCGCCGCCGGAAGCTTTTGGTGTCGCGCAAGGAAATGGCGGATCTGTGGAACGCAACCCAGCGCAAGGGGAT
GACTCTTGTGCCGCTGGTTCTCTATTTCAACCATCGTGGCATGGCGAAGATCAAGATCGGCATCGCGAAGGGTAAGAAGA
ACCACGACAAGCGCGAAGCCCAGGCCAAACGCGATTGGTCACGCCAGAAACAACGCCTTCTCAAGGATCACGGCTAA

Upstream 100 bases:

>100_bases
CGTGAAAGCGCGCCGAGCATGGCCTTTGCAGCACAGAATGCCTGCTTTGTGCATCAGGTGATCTTGCACGCCGTTGCTCG
GCGACTTACATCTGCGCCCC

Downstream 100 bases:

>100_bases
CCAGAGTCTGTGGGTTTCCGCGCGCGCTTGAGTGATCAAGCATGGCTTGCCTACATTCAGATCTCATTGCCAAACAACAA
GCCCCGAAAGAGCGCACTCT

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MAKQKTDPNYKVIAENRRARFDYAIEQDLECGIMLEGSEVKSLRAGGSNIAESYAAVEEGELWLVNSYIAPYEQAKVFKH
EERRRRKLLVSRKEMADLWNATQRKGMTLVPLVLYFNHRGMAKIKIGIAKGKKNHDKREAQAKRDWSRQKQRLLKDHG

Sequences:

>Translated_158_residues
MAKQKTDPNYKVIAENRRARFDYAIEQDLECGIMLEGSEVKSLRAGGSNIAESYAAVEEGELWLVNSYIAPYEQAKVFKH
EERRRRKLLVSRKEMADLWNATQRKGMTLVPLVLYFNHRGMAKIKIGIAKGKKNHDKREAQAKRDWSRQKQRLLKDHG
>Mature_157_residues
AKQKTDPNYKVIAENRRARFDYAIEQDLECGIMLEGSEVKSLRAGGSNIAESYAAVEEGELWLVNSYIAPYEQAKVFKHE
ERRRRKLLVSRKEMADLWNATQRKGMTLVPLVLYFNHRGMAKIKIGIAKGKKNHDKREAQAKRDWSRQKQRLLKDHG

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=158, Percent_Identity=42.4050632911392, Blast_Score=119, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_SILST (Q1GDQ2)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614476.1
- ProteinModelPortal:   Q1GDQ2
- SMR:   Q1GDQ2
- STRING:   Q1GDQ2
- GeneID:   4076847
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2482
- NMPDR:   fig|292414.1.peg.799
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   Q1GDQ2
- ProtClustDB:   PRK05422
- BioCyc:   SSP292414:TM1040_2482-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18345; Mature: 18214

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKQKTDPNYKVIAENRRARFDYAIEQDLECGIMLEGSEVKSLRAGGSNIAESYAAVEEG
CCCCCCCCCEEEEECCCCCCCCHHHCCCCCCCEEECCCHHHHHHCCCCHHHHHHHHHCCC
ELWLVNSYIAPYEQAKVFKHEERRRRKLLVSRKEMADLWNATQRKGMTLVPLVLYFNHRG
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
MAKIKIGIAKGKKNHDKREAQAKRDWSRQKQRLLKDHG
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKQKTDPNYKVIAENRRARFDYAIEQDLECGIMLEGSEVKSLRAGGSNIAESYAAVEEG
CCCCCCCCEEEEECCCCCCCCHHHCCCCCCCEEECCCHHHHHHCCCCHHHHHHHHHCCC
ELWLVNSYIAPYEQAKVFKHEERRRRKLLVSRKEMADLWNATQRKGMTLVPLVLYFNHRG
CEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
MAKIKIGIAKGKKNHDKREAQAKRDWSRQKQRLLKDHG
CEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA