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The map label for this gene is ada [H]

Identifier: 99082290

GI number: 99082290

Start: 2591174

End: 2592025

Strand: Reverse

Name: ada [H]

Synonym: TM1040_2450

Alternate gene names: 99082290

Gene position: 2592025-2591174 (Counterclockwise)

Preceding gene: 99082294

Following gene: 99082289

Centisome position: 80.98

GC content: 63.97

Gene sequence:

>852_bases
ATGCAACGGGATGAACAGGCGTACCATTACGGGGTGATCCGGCGCGCTATCGAGGTCATCGATGCGGATGGTGGCGATCT
GTCGCTGGAGGAGCTGGCGGCGCGCATGGGCATGAGCGCCGCGCATTTCCAGCGCGTCTTTTCCTCTTGGGCTGGCGTTT
CCCCCAAGCGGTATCAGCAATATTTGCGCCTTGGTCATGCCAAAGCGCTGCTCCACGAGGATCTGCCTCTTCTGGAGACC
GCACATGCGGTGGGCCTTTCTGGATCGGGACGGTTGCACGACCTCTTTGTCCGATGGGAGGCCATGAGCCCAGGAGATTA
TGCCCGCCGTGGCGCGGGGCTTACTGTGCGCTGGGGGTGGTTTGACAGCCCCTTTGGGCAGGCGCTTACGATGGGGACAG
AGAAGGGGCTCTGTGGGCTGGCCTTTGCCGCCGAGGTTGGGCCAGAGGCCGCTTGGGAGGACATGCGCGCCCGCTGGCCG
AAGGCGGAGTTTGTCGAAGACCCCGATGCCCTTCGCCCGCGGGTTGAGGCCGCCTTTGCCCAAAAGGGAGAAACCGCCCT
CCATCTGATTGGCGCCCCGCTGCAGATAAAGGTCTGGGAGGCGCTGTTGCAGATCCCTGAAGGTCAAGCGACCACCTATG
GCGATATCGCGGAGGCGATCGATAACCCACGTGCCGTGCGCGCCGTTGGCACGGCCGTGGGGAAGAATCCGCTCAGCTGG
CTCATTCCCTGCCATCGCGTCCTTCGCAAAGGCGGAGGCATGGGGGGCTATCACTGGGGTGTGCCCGTCAAACGTGCGAT
GCTGGCTTTTGAGGCGGCCCGTACCGAGACCGACCCGAAGGCCGTCGCCTGA

Upstream 100 bases:

>100_bases
GCCTGGACGGCCAAACCGCAGGTTCCGGGTCGCCGCCGCGCGGCACGTTGGCTAAGGTGCGTCTCCAGAGTATTGACCCA
ATACAAGAGGACGAGGTGCC

Downstream 100 bases:

>100_bases
ACGGCACGCGGATTGACAGGTTTATCATTTTCATCAAGGCTTTGCGCCTTGTGCGCCTTGTGCGCCTTGTGCGCCGTTTC
GCCCGTTGAAGGGCCTCGAT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MQRDEQAYHYGVIRRAIEVIDADGGDLSLEELAARMGMSAAHFQRVFSSWAGVSPKRYQQYLRLGHAKALLHEDLPLLET
AHAVGLSGSGRLHDLFVRWEAMSPGDYARRGAGLTVRWGWFDSPFGQALTMGTEKGLCGLAFAAEVGPEAAWEDMRARWP
KAEFVEDPDALRPRVEAAFAQKGETALHLIGAPLQIKVWEALLQIPEGQATTYGDIAEAIDNPRAVRAVGTAVGKNPLSW
LIPCHRVLRKGGGMGGYHWGVPVKRAMLAFEAARTETDPKAVA

Sequences:

>Translated_283_residues
MQRDEQAYHYGVIRRAIEVIDADGGDLSLEELAARMGMSAAHFQRVFSSWAGVSPKRYQQYLRLGHAKALLHEDLPLLET
AHAVGLSGSGRLHDLFVRWEAMSPGDYARRGAGLTVRWGWFDSPFGQALTMGTEKGLCGLAFAAEVGPEAAWEDMRARWP
KAEFVEDPDALRPRVEAAFAQKGETALHLIGAPLQIKVWEALLQIPEGQATTYGDIAEAIDNPRAVRAVGTAVGKNPLSW
LIPCHRVLRKGGGMGGYHWGVPVKRAMLAFEAARTETDPKAVA
>Mature_283_residues
MQRDEQAYHYGVIRRAIEVIDADGGDLSLEELAARMGMSAAHFQRVFSSWAGVSPKRYQQYLRLGHAKALLHEDLPLLET
AHAVGLSGSGRLHDLFVRWEAMSPGDYARRGAGLTVRWGWFDSPFGQALTMGTEKGLCGLAFAAEVGPEAAWEDMRARWP
KAEFVEDPDALRPRVEAAFAQKGETALHLIGAPLQIKVWEALLQIPEGQATTYGDIAEAIDNPRAVRAVGTAVGKNPLSW
LIPCHRVLRKGGGMGGYHWGVPVKRAMLAFEAARTETDPKAVA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=279, Percent_Identity=27.5985663082437, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787596, Length=81, Percent_Identity=48.1481481481481, Blast_Score=78, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115533068, Length=133, Percent_Identity=33.0827067669173, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=132, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=87, Percent_Identity=41.3793103448276, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17137554, Length=175, Percent_Identity=33.1428571428571, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 30966; Mature: 30966

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRDEQAYHYGVIRRAIEVIDADGGDLSLEELAARMGMSAAHFQRVFSSWAGVSPKRYQQ
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHH
YLRLGHAKALLHEDLPLLETAHAVGLSGSGRLHDLFVRWEAMSPGDYARRGAGLTVRWGW
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHCCCCCEEEECC
FDSPFGQALTMGTEKGLCGLAFAAEVGPEAAWEDMRARWPKAEFVEDPDALRPRVEAAFA
CCCCHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHH
QKGETALHLIGAPLQIKVWEALLQIPEGQATTYGDIAEAIDNPRAVRAVGTAVGKNPLSW
HCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHH
LIPCHRVLRKGGGMGGYHWGVPVKRAMLAFEAARTETDPKAVA
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQRDEQAYHYGVIRRAIEVIDADGGDLSLEELAARMGMSAAHFQRVFSSWAGVSPKRYQQ
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHH
YLRLGHAKALLHEDLPLLETAHAVGLSGSGRLHDLFVRWEAMSPGDYARRGAGLTVRWGW
HHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEEEECCCCCHHHHCCCCCEEEECC
FDSPFGQALTMGTEKGLCGLAFAAEVGPEAAWEDMRARWPKAEFVEDPDALRPRVEAAFA
CCCCHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHH
QKGETALHLIGAPLQIKVWEALLQIPEGQATTYGDIAEAIDNPRAVRAVGTAVGKNPLSW
HCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHH
LIPCHRVLRKGGGMGGYHWGVPVKRAMLAFEAARTETDPKAVA
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904855; 11677609 [H]