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The map label for this gene is rlmL [H]

Identifier: 99082255

GI number: 99082255

Start: 2555077

End: 2556198

Strand: Reverse

Name: rlmL [H]

Synonym: TM1040_2415

Alternate gene names: 99082255

Gene position: 2556198-2555077 (Counterclockwise)

Preceding gene: 99082264

Following gene: 99082254

Centisome position: 79.86

GC content: 61.68

Gene sequence:

>1122_bases
ATGATGGCTGACACCTCCTTTGAAATCTTTCTCGTGGCTCCTCCGGGGCTGGAACATGTCCTGTGTGAAGAGGCCCGCGA
GGCCGGGTTTGAAGCGGCGCGCCAAGTTGCTGGGGGTGTGAGCTTTGAAGGCACTTGGGAGGACGTGCGACGCGCCAATC
TGGTGCTGCGTGGTGCGACGCGTGTTCTGGCGCGGATCGGTTCTTTTCGCGCCTTCCACCTTGCCCAGCTGGATAAGCGC
GCGCGAAAATTCCCGTGGGGGGATGTTCTGCGCAAGGACGTACCGGTCAAGGTTGAGGTGGTCACCAACAAGAAGTCCAA
GATCTATCATGCGGGTGCGGCCACACAGCGGATCGAGACCGCCATCACCGAGGAACTCGGTGCGCCAATCGCCCCTGATG
CGCCGGTGACGCTCAAGGTTCGGATTGACGACAACACCTGTATTTTCAGTGTCGATACCTCTGGCGAGGGCCTGCATAAA
CGCGGCCACAAGGTCGCTGTTGGCAAGGCCCCGATGCGCGAAACCCTCGCGGCGCTGCTGTTGCGCGCCTGCGGCTATGA
CGGGAGCGAACCTGTCGTGGACCCGATGTGCGGGTCGGGGACATTTGTGATCGAGGCGGCAGAAATCGCGTCCGGCCTCA
CTGCGGGGCGCAGCCGGTCGTTTGCCTTTGAGCAGCTAGCAAATCACGACGCGACCGCTTGGACTGATCTCAAGGCAAGC
ATGCCAGAGGCGGTTCCCACCGTGTGTCGGTATTTTGGTTCGGATCGAAATACGGGTGCCATCGAGATGGCTAGTCAGAA
CGCCGACCGCGCTGGCGTGGAGGGGATTTGCCACTTTACCCATGCCGCCGTCAGTGACCTGCAACGTCCAGAAGGTCCGG
CGGGGCTCGTGATGGTCAATCCCCCCTATGGGGCAAGGATTGGCAACAAAAAACAGCTGTTTGCGGTCTATGGCGCCATG
GGCGAGGTGTTGAAAACGCGGTTTTCCGGGTGGCGCGTCGGGATCGTCACCAGCGATACCGCCCTTGCGCGCGCCACCGG
CTTGCCATTCTTGCCCGTTGGCGCGCCAATTGCGCACGGCGGCTTGAAGGTGCGCCTGTTTCAGACCGCCCCTCTCCCCT
AA

Upstream 100 bases:

>100_bases
TGCGGGGGAGGCACGCGCCTGTGCGTCACTGTGGGCGCGCGTTTTGACGATGTCTCATTAGGGATACCCCCGGCCAGTGA
TTCCAGCTAAGAGGTGGCGC

Downstream 100 bases:

>100_bases
GCGCGGGGCCTCAACCCCGCACCATCCGTTGCGTCACGCAGAAGATCGCGGCTTGCAAAGACAGGTCCGCGCGGCTCTCT
TGTGACGCGAGAAGTGCCAA

Product: putative RNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MMADTSFEIFLVAPPGLEHVLCEEAREAGFEAARQVAGGVSFEGTWEDVRRANLVLRGATRVLARIGSFRAFHLAQLDKR
ARKFPWGDVLRKDVPVKVEVVTNKKSKIYHAGAATQRIETAITEELGAPIAPDAPVTLKVRIDDNTCIFSVDTSGEGLHK
RGHKVAVGKAPMRETLAALLLRACGYDGSEPVVDPMCGSGTFVIEAAEIASGLTAGRSRSFAFEQLANHDATAWTDLKAS
MPEAVPTVCRYFGSDRNTGAIEMASQNADRAGVEGICHFTHAAVSDLQRPEGPAGLVMVNPPYGARIGNKKQLFAVYGAM
GEVLKTRFSGWRVGIVTSDTALARATGLPFLPVGAPIAHGGLKVRLFQTAPLP

Sequences:

>Translated_373_residues
MMADTSFEIFLVAPPGLEHVLCEEAREAGFEAARQVAGGVSFEGTWEDVRRANLVLRGATRVLARIGSFRAFHLAQLDKR
ARKFPWGDVLRKDVPVKVEVVTNKKSKIYHAGAATQRIETAITEELGAPIAPDAPVTLKVRIDDNTCIFSVDTSGEGLHK
RGHKVAVGKAPMRETLAALLLRACGYDGSEPVVDPMCGSGTFVIEAAEIASGLTAGRSRSFAFEQLANHDATAWTDLKAS
MPEAVPTVCRYFGSDRNTGAIEMASQNADRAGVEGICHFTHAAVSDLQRPEGPAGLVMVNPPYGARIGNKKQLFAVYGAM
GEVLKTRFSGWRVGIVTSDTALARATGLPFLPVGAPIAHGGLKVRLFQTAPLP
>Mature_373_residues
MMADTSFEIFLVAPPGLEHVLCEEAREAGFEAARQVAGGVSFEGTWEDVRRANLVLRGATRVLARIGSFRAFHLAQLDKR
ARKFPWGDVLRKDVPVKVEVVTNKKSKIYHAGAATQRIETAITEELGAPIAPDAPVTLKVRIDDNTCIFSVDTSGEGLHK
RGHKVAVGKAPMRETLAALLLRACGYDGSEPVVDPMCGSGTFVIEAAEIASGLTAGRSRSFAFEQLANHDATAWTDLKAS
MPEAVPTVCRYFGSDRNTGAIEMASQNADRAGVEGICHFTHAAVSDLQRPEGPAGLVMVNPPYGARIGNKKQLFAVYGAM
GEVLKTRFSGWRVGIVTSDTALARATGLPFLPVGAPIAHGGLKVRLFQTAPLP

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=322, Percent_Identity=29.8136645962733, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 39890; Mature: 39890

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMADTSFEIFLVAPPGLEHVLCEEAREAGFEAARQVAGGVSFEGTWEDVRRANLVLRGAT
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
RVLARIGSFRAFHLAQLDKRARKFPWGDVLRKDVPVKVEVVTNKKSKIYHAGAATQRIET
HHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEECCCCEEEECCCHHHHHHH
AITEELGAPIAPDAPVTLKVRIDDNTCIFSVDTSGEGLHKRGHKVAVGKAPMRETLAALL
HHHHHHCCCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHCCCEEEECCCCHHHHHHHHH
LRACGYDGSEPVVDPMCGSGTFVIEAAEIASGLTAGRSRSFAFEQLANHDATAWTDLKAS
HHHHCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHCCCCCCHHHHHHHCCCCCEEHHHHHC
MPEAVPTVCRYFGSDRNTGAIEMASQNADRAGVEGICHFTHAAVSDLQRPEGPAGLVMVN
CCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PPYGARIGNKKQLFAVYGAMGEVLKTRFSGWRVGIVTSDTALARATGLPFLPVGAPIAHG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHCCCCEECCCCCEECC
GLKVRLFQTAPLP
CEEEEEEECCCCC
>Mature Secondary Structure
MMADTSFEIFLVAPPGLEHVLCEEAREAGFEAARQVAGGVSFEGTWEDVRRANLVLRGAT
CCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
RVLARIGSFRAFHLAQLDKRARKFPWGDVLRKDVPVKVEVVTNKKSKIYHAGAATQRIET
HHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEECCCCEEEECCCHHHHHHH
AITEELGAPIAPDAPVTLKVRIDDNTCIFSVDTSGEGLHKRGHKVAVGKAPMRETLAALL
HHHHHHCCCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHCCCEEEECCCCHHHHHHHHH
LRACGYDGSEPVVDPMCGSGTFVIEAAEIASGLTAGRSRSFAFEQLANHDATAWTDLKAS
HHHHCCCCCCCCCCCCCCCCEEEEEHHHHHCCHHCCCCCCHHHHHHHCCCCCEEHHHHHC
MPEAVPTVCRYFGSDRNTGAIEMASQNADRAGVEGICHFTHAAVSDLQRPEGPAGLVMVN
CCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEC
PPYGARIGNKKQLFAVYGAMGEVLKTRFSGWRVGIVTSDTALARATGLPFLPVGAPIAHG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHCCCCEECCCCCEECC
GLKVRLFQTAPLP
CEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA