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The map label for this gene is gap [H]

Identifier: 99082234

GI number: 99082234

Start: 2517618

End: 2518607

Strand: Direct

Name: gap [H]

Synonym: TM1040_2394

Alternate gene names: 99082234

Gene position: 2517618-2518607 (Clockwise)

Preceding gene: 99082229

Following gene: 99082235

Centisome position: 78.65

GC content: 62.12

Gene sequence:

>990_bases
ATGAAGATAGCGATCAACGGATTCGGCCGCATTGGCCGTACAATCTTGCGCCAGATCCTGACCAGCCCGCGCGGCTCCGG
GATCGAGGTGGTTTGCATCAACGACATCACCGATGCGGAGATGTGCGCCTATCTGTTCCAATACGACAGCACCTTTGGCC
CCTACCCGGGCGAGGTGTCGCTCGAGGCGGGTACGCTCTGCGTCGACGGACGGCGGATCGCGCTTCACCAAGAGGCCAAT
GTGACCAACCTCGACCTCGCGGGTGTGGATGTCTTGATGGAATGCACAGGCATTGCACGCACGACCGACCTGGCACAGCG
CGGCCTGACCGCAGGAGCGCGCAAGGTTTTGATTTCCGGACCATCTCCGGCTGCCGAAGTCACCGTTGTATATGGCGTCA
ATGATGCTGACCTTGCAGGCGCGCAGATCGTGTCGAACGCCTCTTGCACCACCAATGCGGTTGCAGGGCTGGTGCAGCTG
GTGGATGCGGTCGCCGGAATTGAAACCGGTCACATGACCACCATCCACTGCTATACCAACTCGCAACCGATGGTGGACAC
ACCGCGCGGCGACTTCGCCCGGTCGCGGGGAGGCGCACAGTCCATGGTGCCCACGACAACCTCCGCAATGCATCTGATCG
ACGTCGTTCTGCCTCGCCTCAAGGGGCGGATTTCCGGCGCCGCCGTACGGGTTCCGACCGCCAGCGTTTCGGCGGTGGAT
CTCGTGGCAACCCTGCAATCAGAATGGGATCAATCCGATCTCCTGATGGCCCTGAAGGCATCGGCCAACGGCTCGCCCAT
CTTGGGATGGACCGAACAGCCGCTCGTTTCTTCGGACCTGCGGGCGCGGCCAGAATCGCTGATTATCGCAGCCCCCGAAA
CCCGCATGGTGGGGACGCAGCAGCTGCGCATCTTCGGCTGGTATGACAATGAATGGGGCTTTTCGGCGCGGATGCTTGAT
GTCGCGCGCGCGATGCAGCCCCAGGTCTGA

Upstream 100 bases:

>100_bases
TTCTGCGCCCGGGCTCCCTCCAGACGCGATTGTATCAAGCCTGCCGCATTATTGTGCGGGTGGATTGCCGGGCGCAAAAA
GCGCTGCCATAGCTTTGCCC

Downstream 100 bases:

>100_bases
TCGGCGCATTTTCGCCACGCCACTACAGCAATTCTCCTCTACATGGGCTCGACCCGGCGGGCCGAGGTTTTATGTCACCG
GCCATGCGTGTAGCCTCATT

Product: glyceraldehyde 3-phosphate dehydrogenase (phosphorylating)

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKIAINGFGRIGRTILRQILTSPRGSGIEVVCINDITDAEMCAYLFQYDSTFGPYPGEVSLEAGTLCVDGRRIALHQEAN
VTNLDLAGVDVLMECTGIARTTDLAQRGLTAGARKVLISGPSPAAEVTVVYGVNDADLAGAQIVSNASCTTNAVAGLVQL
VDAVAGIETGHMTTIHCYTNSQPMVDTPRGDFARSRGGAQSMVPTTTSAMHLIDVVLPRLKGRISGAAVRVPTASVSAVD
LVATLQSEWDQSDLLMALKASANGSPILGWTEQPLVSSDLRARPESLIIAAPETRMVGTQQLRIFGWYDNEWGFSARMLD
VARAMQPQV

Sequences:

>Translated_329_residues
MKIAINGFGRIGRTILRQILTSPRGSGIEVVCINDITDAEMCAYLFQYDSTFGPYPGEVSLEAGTLCVDGRRIALHQEAN
VTNLDLAGVDVLMECTGIARTTDLAQRGLTAGARKVLISGPSPAAEVTVVYGVNDADLAGAQIVSNASCTTNAVAGLVQL
VDAVAGIETGHMTTIHCYTNSQPMVDTPRGDFARSRGGAQSMVPTTTSAMHLIDVVLPRLKGRISGAAVRVPTASVSAVD
LVATLQSEWDQSDLLMALKASANGSPILGWTEQPLVSSDLRARPESLIIAAPETRMVGTQQLRIFGWYDNEWGFSARMLD
VARAMQPQV
>Mature_329_residues
MKIAINGFGRIGRTILRQILTSPRGSGIEVVCINDITDAEMCAYLFQYDSTFGPYPGEVSLEAGTLCVDGRRIALHQEAN
VTNLDLAGVDVLMECTGIARTTDLAQRGLTAGARKVLISGPSPAAEVTVVYGVNDADLAGAQIVSNASCTTNAVAGLVQL
VDAVAGIETGHMTTIHCYTNSQPMVDTPRGDFARSRGGAQSMVPTTTSAMHLIDVVLPRLKGRISGAAVRVPTASVSAVD
LVATLQSEWDQSDLLMALKASANGSPILGWTEQPLVSSDLRARPESLIIAAPETRMVGTQQLRIFGWYDNEWGFSARMLD
VARAMQPQV

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7657116, Length=333, Percent_Identity=39.3393393393393, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI7669492, Length=335, Percent_Identity=37.3134328358209, Blast_Score=218, Evalue=9e-57,
Organism=Escherichia coli, GI1788079, Length=326, Percent_Identity=40.1840490797546, Blast_Score=237, Evalue=6e-64,
Organism=Escherichia coli, GI1789295, Length=331, Percent_Identity=39.5770392749245, Blast_Score=232, Evalue=3e-62,
Organism=Caenorhabditis elegans, GI17568413, Length=332, Percent_Identity=38.5542168674699, Blast_Score=207, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI32566163, Length=332, Percent_Identity=38.5542168674699, Blast_Score=207, Evalue=5e-54,
Organism=Caenorhabditis elegans, GI17534677, Length=332, Percent_Identity=37.3493975903614, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17534679, Length=332, Percent_Identity=36.7469879518072, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6321631, Length=329, Percent_Identity=40.7294832826748, Blast_Score=238, Evalue=8e-64,
Organism=Saccharomyces cerevisiae, GI6322468, Length=328, Percent_Identity=39.9390243902439, Blast_Score=233, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6322409, Length=328, Percent_Identity=40.5487804878049, Blast_Score=231, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17933600, Length=334, Percent_Identity=38.9221556886228, Blast_Score=223, Evalue=9e-59,
Organism=Drosophila melanogaster, GI18110149, Length=334, Percent_Identity=38.9221556886228, Blast_Score=223, Evalue=9e-59,
Organism=Drosophila melanogaster, GI85725000, Length=334, Percent_Identity=38.3233532934132, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI22023983, Length=334, Percent_Identity=38.3233532934132, Blast_Score=218, Evalue=5e-57,
Organism=Drosophila melanogaster, GI19922412, Length=331, Percent_Identity=36.5558912386707, Blast_Score=200, Evalue=1e-51,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 34919; Mature: 34919

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAINGFGRIGRTILRQILTSPRGSGIEVVCINDITDAEMCAYLFQYDSTFGPYPGEVS
CEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEE
LEAGTLCVDGRRIALHQEANVTNLDLAGVDVLMECTGIARTTDLAQRGLTAGARKVLISG
EECCEEEECCCEEEEEECCCCCEEEHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEC
PSPAAEVTVVYGVNDADLAGAQIVSNASCTTNAVAGLVQLVDAVAGIETGHMTTIHCYTN
CCCCCEEEEEEECCCCHHCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEC
SQPMVDTPRGDFARSRGGAQSMVPTTTSAMHLIDVVLPRLKGRISGAAVRVPTASVSAVD
CCCCEECCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHH
LVATLQSEWDQSDLLMALKASANGSPILGWTEQPLVSSDLRARPESLIIAAPETRMVGTQ
HHHHHHHCCCCCCEEEEEECCCCCCEEECCCCCCCCCCHHHCCCCEEEEECCCCCCCCCE
QLRIFGWYDNEWGFSARMLDVARAMQPQV
EEEEEEEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIAINGFGRIGRTILRQILTSPRGSGIEVVCINDITDAEMCAYLFQYDSTFGPYPGEVS
CEEEECCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEE
LEAGTLCVDGRRIALHQEANVTNLDLAGVDVLMECTGIARTTDLAQRGLTAGARKVLISG
EECCEEEECCCEEEEEECCCCCEEEHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEC
PSPAAEVTVVYGVNDADLAGAQIVSNASCTTNAVAGLVQLVDAVAGIETGHMTTIHCYTN
CCCCCEEEEEEECCCCHHCCHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEC
SQPMVDTPRGDFARSRGGAQSMVPTTTSAMHLIDVVLPRLKGRISGAAVRVPTASVSAVD
CCCCEECCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCHHHH
LVATLQSEWDQSDLLMALKASANGSPILGWTEQPLVSSDLRARPESLIIAAPETRMVGTQ
HHHHHHHCCCCCCEEEEEECCCCCCEEECCCCCCCCCCHHHCCCCEEEEECCCCCCCCCE
QLRIFGWYDNEWGFSARMLDVARAMQPQV
EEEEEEEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045900; 10984043 [H]