| Definition | Ruegeria sp. TM1040, complete genome. |
|---|---|
| Accession | NC_008044 |
| Length | 3,200,938 |
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The map label for this gene is topA [H]
Identifier: 99082179
GI number: 99082179
Start: 2459369
End: 2462050
Strand: Direct
Name: topA [H]
Synonym: TM1040_2339
Alternate gene names: 99082179
Gene position: 2459369-2462050 (Clockwise)
Preceding gene: 99082178
Following gene: 99082180
Centisome position: 76.83
GC content: 60.7
Gene sequence:
>2682_bases ATGCCAGTTGTCGTTGTCGAATCCCCGGCCAAAGCCAAAACAATCAATAAATATCTGGGCTCCGACTACACTGTTTTGGC ATCCTACGGTCACGTCCGGGACCTGCCCGCAAAGAACGGATCCGTTGACCCCGAAGATGATTTTGCGATGACATGGGAGG TCGGCAACGACTCCAAAAAACATGTGCGCGCCATCGCAGAGGCGCTCAAGGACGACAACGCATTGATCCTCGCGACCGAC CCCGACCGCGAAGGCGAGGCGATCAGCTGGCACTTGCAAGAAGCGCTCACCAAGCGGCGCTCCATCAAGAAGGACACCGA CGTCAAACGCGTGACCTTCAACGCGATCACCAAGGACGCTGTCGCCGAGGCCATGAAAAACCCCCGCGATGTCGATATGC CGCTGGTAGAGGCCTACCTCGCCCGGCGTGCTCTCGATTATCTTGTGGGCTTCAACCTCTCTCCGGTGCTTTGGCGCAAA CTGCCCGGCGCGCGCTCGGCCGGGCGTGTGCAATCGGTTTGCCTCCGCCTCATCGTGGAGCGCGAGATGGAGATCGAGGC GTTCAACCCGCAGGAATATTGGTCCGTCCGCGCAAACCTTGCCACCCCGCGCGGCCAATCGTTTGAGGCGCGCCTGACCG TGCTCGGCGGTGAAAAACTCGACAAATTCAGCCTCGCAAACTCGACGGCGGCAGAACTGGCGGTGCAGGCCGTCAGCAGC CGCAAAATGTCTGTCCTGTCGGTTGAGGCCAAACCGGCGGCGCGCAACCCCTCTGCGCCTTTCATGACCTCGACCCTGCA ACAGGAAGCGAGCCGCAAGTTCGGCATGGGCGCCAAACAGTGCATGAATGCCGCGCAGCGTCTCTATGAGGCCGGGCTCA TCACCTATATGCGGACCGATGGGATCGACATGGCCCCGGAGGCGGTCTCTGCCGCGCGCGATGAGATTGCCAAGCGCTAT GGCCAGGAATATGTGCCCGGCGAGCCGCGGATCTACAAGAACAAGGCCAAGAACGCCCAAGAGGCGCACGAATGTATCCG CCCGACCGACATGAGCCGCGACGCCGCGAGCCTCAAAGTATCCGAAGATGATCAGCGCAAGCTTTATGACCTGATCTGGA AACGCACGCTCGCCTGCCAGATGGCAGGTGCCCGTCTGGAGCGCACCACAGTGGACATCGGCTCTGACGACAAGCAGGTG GTGCTGCGCGCCACCGGTCAGGTCGTCATGTTTGATGGCTTCATGCGCGTGTATGAAGAAGGCCGCGATGATGTGGTCGA CGAGGACGACAAGCGTCTGCCGCAGATCATGCAAGGCGAAGCACTGAAGTTTGCAGGTTCGCTTGCGGCTCAGGCGGAAA AAGCGGGCAAAGACGGTTCTGTGCTGTCGCAGGATGGCGCAACGCTGGCACTGCAGCACCACACCCAGCCGCCGCCGCGC TATACCGAGGCCACATTGGTCAAACGGATGGAAGAGCTCGGCATCGGCCGCCCGTCGACCTACGCCAGCGTCATCACCAC GATCCAGGACCGCGAATATGTCCGCAAGGACAAGAACCGCCTCTTCCCCGAGGACAAAGGGCGGATCGTGACCATCTTCC TGCTGAACTTCTTTAAGCGCTACGTGGAATATGACTTCACCGCCGCGCTCGAGAACCAGCTCGATGATGTCAGTGCAGGC GGCAAGGACTACAAGGAACTTCTGGACAGTTTCTGGCGCGACTTCTCGGCGGCGATCTCCGAGACCTCCGATCTGCGCAT CTCGGAAGTTCTGGATGTGCTGGACGAGGCGCTTGCGCCACAGCTCTACCCCCCGCGAGAAGATGGTGGCGACCCGCGTA TCTGCCCCAAGTGTGGTGCCGGTCAACTACATTTGAAAACCTCGCGCACCGGCGGTTTCGTGGGTTGCGGCAACTACCCC GAATGCAACTATACCCGCCCGATCTCGGGCGAGGGCGCCGAAGGTTATGAACGCGTCCTCGGCGAGGATGACGGCGACGA GATCCACCTGAAGTCCGGCCGCTTTGGCCCCTATGTGCAACGCGGTGAGGCCACGCCAGAAAACAAGAAGCCGCCGCGCT CCTCGCTGCCGAAACAGGGCAAGGACTATCTGCCCGGCTGGGGCCCCAATGAGATCACGCTCGAGCAAGCCGTGACCTTG CTGACGCTACCGCGCGAAATCGGCGCGCACCCGGAGGGCGGCGTGGTGTCGTCGAACCTCGGTCGATTTGGCCCCTACAT CATGCACCAGCGTCCCGACGAGGAAAAACCGGTCTATGTGAACCTGAAGGAAACGCTGGACGTCTTTGAAATCGGCATGA ACCGCGCGGTGGAGATGCTGGCGGAAAAACGCGCCAACCCGGGCCGCGGGCGCTCTGCTGCCGCAAAACCGCTGCGTGAG CTGGGCGAACATCCGGACGACAAGGCGCCCGTGAACATCATGGATGGGCGCTACGGGCCTTATGTGAAATGGGGCAAGGT CAATGCGACTCTGCCCAAGGACACCGAGGCCAAGGACGTGACCATGGAGATGGCGGTTGAGCTTCTGAATGAAAAAGCCG CCAAATCCGGCAAGAAAAAAGCGCCCGCGAAGAAAGCTGCTGCGAAGAAGACCACAGCCAAGAAAACCACCGCGAAAAAA GCACCCGCCAAAAAGAAAGAGGCCAAAGCCTCTGGCGATTAA
Upstream 100 bases:
>100_bases GCGCTGATTTCTTCGCGTGGATTGACATTCTCCCCTTGCGCGACACATTTTGCCCGGCCATAGAGCGCGATTGAACCCGT CTGAAAAGGTGCCCACTTAC
Downstream 100 bases:
>100_bases CCGCAGGCGGGGCGCGCTCCCGCCCGTCGTCGCTGCACCAGGATGCAGGTCCTCCCGTTGGGACCAGCGCCGCGCTTTCG CGCGGCGTTTGTGTATTTCG
Product: DNA topoisomerase I
Products: NA
Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]
Number of amino acids: Translated: 893; Mature: 892
Protein sequence:
>893_residues MPVVVVESPAKAKTINKYLGSDYTVLASYGHVRDLPAKNGSVDPEDDFAMTWEVGNDSKKHVRAIAEALKDDNALILATD PDREGEAISWHLQEALTKRRSIKKDTDVKRVTFNAITKDAVAEAMKNPRDVDMPLVEAYLARRALDYLVGFNLSPVLWRK LPGARSAGRVQSVCLRLIVEREMEIEAFNPQEYWSVRANLATPRGQSFEARLTVLGGEKLDKFSLANSTAAELAVQAVSS RKMSVLSVEAKPAARNPSAPFMTSTLQQEASRKFGMGAKQCMNAAQRLYEAGLITYMRTDGIDMAPEAVSAARDEIAKRY GQEYVPGEPRIYKNKAKNAQEAHECIRPTDMSRDAASLKVSEDDQRKLYDLIWKRTLACQMAGARLERTTVDIGSDDKQV VLRATGQVVMFDGFMRVYEEGRDDVVDEDDKRLPQIMQGEALKFAGSLAAQAEKAGKDGSVLSQDGATLALQHHTQPPPR YTEATLVKRMEELGIGRPSTYASVITTIQDREYVRKDKNRLFPEDKGRIVTIFLLNFFKRYVEYDFTAALENQLDDVSAG GKDYKELLDSFWRDFSAAISETSDLRISEVLDVLDEALAPQLYPPREDGGDPRICPKCGAGQLHLKTSRTGGFVGCGNYP ECNYTRPISGEGAEGYERVLGEDDGDEIHLKSGRFGPYVQRGEATPENKKPPRSSLPKQGKDYLPGWGPNEITLEQAVTL LTLPREIGAHPEGGVVSSNLGRFGPYIMHQRPDEEKPVYVNLKETLDVFEIGMNRAVEMLAEKRANPGRGRSAAAKPLRE LGEHPDDKAPVNIMDGRYGPYVKWGKVNATLPKDTEAKDVTMEMAVELLNEKAAKSGKKKAPAKKAAAKKTTAKKTTAKK APAKKKEAKASGD
Sequences:
>Translated_893_residues MPVVVVESPAKAKTINKYLGSDYTVLASYGHVRDLPAKNGSVDPEDDFAMTWEVGNDSKKHVRAIAEALKDDNALILATD PDREGEAISWHLQEALTKRRSIKKDTDVKRVTFNAITKDAVAEAMKNPRDVDMPLVEAYLARRALDYLVGFNLSPVLWRK LPGARSAGRVQSVCLRLIVEREMEIEAFNPQEYWSVRANLATPRGQSFEARLTVLGGEKLDKFSLANSTAAELAVQAVSS RKMSVLSVEAKPAARNPSAPFMTSTLQQEASRKFGMGAKQCMNAAQRLYEAGLITYMRTDGIDMAPEAVSAARDEIAKRY GQEYVPGEPRIYKNKAKNAQEAHECIRPTDMSRDAASLKVSEDDQRKLYDLIWKRTLACQMAGARLERTTVDIGSDDKQV VLRATGQVVMFDGFMRVYEEGRDDVVDEDDKRLPQIMQGEALKFAGSLAAQAEKAGKDGSVLSQDGATLALQHHTQPPPR YTEATLVKRMEELGIGRPSTYASVITTIQDREYVRKDKNRLFPEDKGRIVTIFLLNFFKRYVEYDFTAALENQLDDVSAG GKDYKELLDSFWRDFSAAISETSDLRISEVLDVLDEALAPQLYPPREDGGDPRICPKCGAGQLHLKTSRTGGFVGCGNYP ECNYTRPISGEGAEGYERVLGEDDGDEIHLKSGRFGPYVQRGEATPENKKPPRSSLPKQGKDYLPGWGPNEITLEQAVTL LTLPREIGAHPEGGVVSSNLGRFGPYIMHQRPDEEKPVYVNLKETLDVFEIGMNRAVEMLAEKRANPGRGRSAAAKPLRE LGEHPDDKAPVNIMDGRYGPYVKWGKVNATLPKDTEAKDVTMEMAVELLNEKAAKSGKKKAPAKKAAAKKTTAKKTTAKK APAKKKEAKASGD >Mature_892_residues PVVVVESPAKAKTINKYLGSDYTVLASYGHVRDLPAKNGSVDPEDDFAMTWEVGNDSKKHVRAIAEALKDDNALILATDP DREGEAISWHLQEALTKRRSIKKDTDVKRVTFNAITKDAVAEAMKNPRDVDMPLVEAYLARRALDYLVGFNLSPVLWRKL PGARSAGRVQSVCLRLIVEREMEIEAFNPQEYWSVRANLATPRGQSFEARLTVLGGEKLDKFSLANSTAAELAVQAVSSR KMSVLSVEAKPAARNPSAPFMTSTLQQEASRKFGMGAKQCMNAAQRLYEAGLITYMRTDGIDMAPEAVSAARDEIAKRYG QEYVPGEPRIYKNKAKNAQEAHECIRPTDMSRDAASLKVSEDDQRKLYDLIWKRTLACQMAGARLERTTVDIGSDDKQVV LRATGQVVMFDGFMRVYEEGRDDVVDEDDKRLPQIMQGEALKFAGSLAAQAEKAGKDGSVLSQDGATLALQHHTQPPPRY TEATLVKRMEELGIGRPSTYASVITTIQDREYVRKDKNRLFPEDKGRIVTIFLLNFFKRYVEYDFTAALENQLDDVSAGG KDYKELLDSFWRDFSAAISETSDLRISEVLDVLDEALAPQLYPPREDGGDPRICPKCGAGQLHLKTSRTGGFVGCGNYPE CNYTRPISGEGAEGYERVLGEDDGDEIHLKSGRFGPYVQRGEATPENKKPPRSSLPKQGKDYLPGWGPNEITLEQAVTLL TLPREIGAHPEGGVVSSNLGRFGPYIMHQRPDEEKPVYVNLKETLDVFEIGMNRAVEMLAEKRANPGRGRSAAAKPLREL GEHPDDKAPVNIMDGRYGPYVKWGKVNATLPKDTEAKDVTMEMAVELLNEKAAKSGKKKAPAKKAAAKKTTAKKTTAKKA PAKKKEAKASGD
Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]
COG id: COG0550
COG function: function code L; Topoisomerase IA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Homo sapiens, GI10835218, Length=606, Percent_Identity=27.3927392739274, Blast_Score=131, Evalue=3e-30, Organism=Homo sapiens, GI4507635, Length=773, Percent_Identity=23.0271668822768, Blast_Score=107, Evalue=7e-23, Organism=Escherichia coli, GI1787529, Length=799, Percent_Identity=37.7972465581977, Blast_Score=467, Evalue=1e-132, Organism=Escherichia coli, GI1788061, Length=547, Percent_Identity=25.2285191956124, Blast_Score=120, Evalue=3e-28, Organism=Caenorhabditis elegans, GI32563869, Length=586, Percent_Identity=25.938566552901, Blast_Score=113, Evalue=4e-25, Organism=Saccharomyces cerevisiae, GI6323263, Length=522, Percent_Identity=26.6283524904215, Blast_Score=121, Evalue=4e-28, Organism=Drosophila melanogaster, GI24585251, Length=649, Percent_Identity=26.1941448382126, Blast_Score=130, Evalue=6e-30,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR003601 - InterPro: IPR013497 - InterPro: IPR013824 - InterPro: IPR013825 - InterPro: IPR000380 - InterPro: IPR003602 - InterPro: IPR013498 - InterPro: IPR005733 - InterPro: IPR006171 [H]
Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]
EC number: =5.99.1.2 [H]
Molecular weight: Translated: 98699; Mature: 98568
Theoretical pI: Translated: 7.98; Mature: 7.98
Prosite motif: PS00396 TOPOISOMERASE_I_PROK
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 2.6 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 2.5 %Met (Mature Protein) 3.4 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPVVVVESPAKAKTINKYLGSDYTVLASYGHVRDLPAKNGSVDPEDDFAMTWEVGNDSKK CCEEEECCCCHHHHHHHHHCCCEEEEECCCCHHCCCCCCCCCCCCCCCEEEEECCCCCHH HVRAIAEALKDDNALILATDPDREGEAISWHLQEALTKRRSIKKDTDVKRVTFNAITKDA HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHH VAEAMKNPRDVDMPLVEAYLARRALDYLVGFNLSPVLWRKLPGARSAGRVQSVCLRLIVE HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH REMEIEAFNPQEYWSVRANLATPRGQSFEARLTVLGGEKLDKFSLANSTAAELAVQAVSS HCCCEEECCCHHHHEEHHCCCCCCCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHC RKMSVLSVEAKPAARNPSAPFMTSTLQQEASRKFGMGAKQCMNAAQRLYEAGLITYMRTD CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECC GIDMAPEAVSAARDEIAKRYGQEYVPGEPRIYKNKAKNAQEAHECIRPTDMSRDAASLKV CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHCCCHHHHHHHCCCCCCCCCCCEEEC SEDDQRKLYDLIWKRTLACQMAGARLERTTVDIGSDDKQVVLRATGQVVMFDGFMRVYEE CCHHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCEEEEEECCCEEEHHHHHHHHHC GRDDVVDEDDKRLPQIMQGEALKFAGSLAAQAEKAGKDGSVLSQDGATLALQHHTQPPPR CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEECCCCCCCC YTEATLVKRMEELGIGRPSTYASVITTIQDREYVRKDKNRLFPEDKGRIVTIFLLNFFKR HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHH YVEYDFTAALENQLDDVSAGGKDYKELLDSFWRDFSAAISETSDLRISEVLDVLDEALAP HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC QLYPPREDGGDPRICPKCGAGQLHLKTSRTGGFVGCGNYPECNYTRPISGEGAEGYERVL CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHH GEDDGDEIHLKSGRFGPYVQRGEATPENKKPPRSSLPKQGKDYLPGWGPNEITLEQAVTL CCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHH LTLPREIGAHPEGGVVSSNLGRFGPYIMHQRPDEEKPVYVNLKETLDVFEIGMNRAVEML HCCHHHHCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH AEKRANPGRGRSAAAKPLRELGEHPDDKAPVNIMDGRYGPYVKWGKVNATLPKDTEAKDV HHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEECCEECCCCCCCCHHHH TMEMAVELLNEKAAKSGKKKAPAKKAAAKKTTAKKTTAKKAPAKKKEAKASGD HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC >Mature Secondary Structure PVVVVESPAKAKTINKYLGSDYTVLASYGHVRDLPAKNGSVDPEDDFAMTWEVGNDSKK CEEEECCCCHHHHHHHHHCCCEEEEECCCCHHCCCCCCCCCCCCCCCEEEEECCCCCHH HVRAIAEALKDDNALILATDPDREGEAISWHLQEALTKRRSIKKDTDVKRVTFNAITKDA HHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHH VAEAMKNPRDVDMPLVEAYLARRALDYLVGFNLSPVLWRKLPGARSAGRVQSVCLRLIVE HHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHH REMEIEAFNPQEYWSVRANLATPRGQSFEARLTVLGGEKLDKFSLANSTAAELAVQAVSS HCCCEEECCCHHHHEEHHCCCCCCCCCCEEEEEEECCCCCHHHHHCCHHHHHHHHHHHHC RKMSVLSVEAKPAARNPSAPFMTSTLQQEASRKFGMGAKQCMNAAQRLYEAGLITYMRTD CCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECC GIDMAPEAVSAARDEIAKRYGQEYVPGEPRIYKNKAKNAQEAHECIRPTDMSRDAASLKV CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHCCCHHHHHHHCCCCCCCCCCCEEEC SEDDQRKLYDLIWKRTLACQMAGARLERTTVDIGSDDKQVVLRATGQVVMFDGFMRVYEE CCHHHHHHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCEEEEEECCCEEEHHHHHHHHHC GRDDVVDEDDKRLPQIMQGEALKFAGSLAAQAEKAGKDGSVLSQDGATLALQHHTQPPPR CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEECCCCCEEEEECCCCCCCC YTEATLVKRMEELGIGRPSTYASVITTIQDREYVRKDKNRLFPEDKGRIVTIFLLNFFKR HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHH YVEYDFTAALENQLDDVSAGGKDYKELLDSFWRDFSAAISETSDLRISEVLDVLDEALAP HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC QLYPPREDGGDPRICPKCGAGQLHLKTSRTGGFVGCGNYPECNYTRPISGEGAEGYERVL CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHH GEDDGDEIHLKSGRFGPYVQRGEATPENKKPPRSSLPKQGKDYLPGWGPNEITLEQAVTL CCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHH LTLPREIGAHPEGGVVSSNLGRFGPYIMHQRPDEEKPVYVNLKETLDVFEIGMNRAVEML HCCHHHHCCCCCCCCCCCCCHHHCCHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH AEKRANPGRGRSAAAKPLRELGEHPDDKAPVNIMDGRYGPYVKWGKVNATLPKDTEAKDV HHHCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCEEEECCEECCCCCCCCHHHH TMEMAVELLNEKAAKSGKKKAPAKKAAAKKTTAKKTTAKKAPAKKKEAKASGD HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA