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The map label for this gene is dacB [H]

Identifier: 99082099

GI number: 99082099

Start: 2373550

End: 2375034

Strand: Direct

Name: dacB [H]

Synonym: TM1040_2259

Alternate gene names: 99082099

Gene position: 2373550-2375034 (Clockwise)

Preceding gene: 99082098

Following gene: 99082101

Centisome position: 74.15

GC content: 64.78

Gene sequence:

>1485_bases
ATGATTTCACGTCGTCACTTTCTCAGCACCGCCCTCGCGGGGCTTGCCGCGGCCCCCGCGCTGGCCAATGCCCCCGGCTA
CACCCCTCGCCCCAAGGCCCGCGGGCTCAGCCCCTATACCCCCGGCGCAGATGGTCTCGACGCGATCCTGCGTGGCGCGG
GGCTCTCAGGGCAGAGCACCTGCGTAGTCGCCAACGCGCGCACCGGTCAGGTCCTCGAAAGTGTGGAGGGCGACGCGCTT
TTACCCCCTGCCAGCGTGGCAAAAGTTCTCACGGCGCTTTACGCGCTCGACTCGCTTGGTGCTGGCTACCGGTTTCGAAC
CCGCCTGGTGGCCACTGGCCCGCTCAAGAACGGGATCATTCAGGGCGATCTTGTGCTGGTTGGCGGCGCCGATCCAACGC
TCGACACGGATGGGCTTGCCAATCTCGCCGGCCAAATGAAGACGGCGGGCGTGCGGGGCGTCACTGGCCGCCTGCTGATC
TGGGAGGATATGCTTCCCTATATCCGGTCGATCGACCCGGACCAGCCCGATCATATCGGCTACAGCCCTTCTGTGAGTGG
CATCGCGCTGAACTTCAACCGGGTTCATTTTGAATGGAAGCGCGCGGGCAACAACTGGGCCATCACGATGGATGCCCGCA
CACAAAAGTACCGCCCCGACGTCTATACCTCGAAAATGGCCGTCGCGCGGCGCGATCTGCCAGTCTACAGCTATGAGAAC
AAGGCGGGCATCGATCATTGGACCGTCGCCAGCGGCGCCCTCGGCAATGGGGGCGCGCGGTGGTTGCCGGTGCGCAATCC
CGCGCTCTATGCCGGGGATGTGTTCCAGACCCTCGCTCGTGCCAACGGGATCGCCTTGCCGAGCCCAAAGACCACCGCGA
CCCTGCCCGGCGGCACCGTGGTCGCCCAGATCCAGAGCGCGCCGCTGCCGGACCTGCTGCGCGCCATGCTCAAATACTCC
AACAACCTGATGGCCGAAATGATCGGCATGACGGCCTCCCTGCAGCGCGGCGCCCGCCCCTCAAGCCTCAAGTCCTCGGG
GCAGAGAATGAGCGACTGGGCGCGCAGCGCCCTCGGCATGCAGCACTGCAAGATGGTGGACCACTCGGGCCTTGGCGAAG
AATCCCGTCTCAGCGCCAATGACATGGTGCGCGCGCTGCTTGCGTCACGCCGCACCGGTGCGCTGAAACCACTGCTCAAG
CCCTACTATTTCAACGATGCCAAGGGCCGCCCGATCAAAACCCACCCTGTGAAGATCGCGGCCAAAACCGGCACGCTCAA
TTTTGTCTCGGGGCTTGGCGGATTTATCAACACGCCCGATGGCGGCGAGCTTGTCTTTGCAATCTTCAGCGCCGACCAGC
GCGCCCGCGCCAAGATCAGCCGTGCCGAACGTGAACGCCCGCGCGGAGGGCGCAGCTGGAACACACGCGCAAAGAAGCTA
CAGCAAAACCTGCTGGACCGGTGGGGACAGGTCTACGGCTCCTGA

Upstream 100 bases:

>100_bases
TAGAGCTGCGCCGTCGAAGCGACACGTCACATAAACTTCTTGCTGGCAAACCATCAAAGCATTTGCGCTCTGTTCGGGAA
TTTGCTCCAAAATACTCAAT

Downstream 100 bases:

>100_bases
ACCCCAGCCGCCGGCACGTCACCCTGTTCTAAACCAAGCCGGAACAAAAAGGGCGCCATCCCGCGCCCTGTTCTGCTCAT
TCTTCTAACCGCAAATATCC

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MISRRHFLSTALAGLAAAPALANAPGYTPRPKARGLSPYTPGADGLDAILRGAGLSGQSTCVVANARTGQVLESVEGDAL
LPPASVAKVLTALYALDSLGAGYRFRTRLVATGPLKNGIIQGDLVLVGGADPTLDTDGLANLAGQMKTAGVRGVTGRLLI
WEDMLPYIRSIDPDQPDHIGYSPSVSGIALNFNRVHFEWKRAGNNWAITMDARTQKYRPDVYTSKMAVARRDLPVYSYEN
KAGIDHWTVASGALGNGGARWLPVRNPALYAGDVFQTLARANGIALPSPKTTATLPGGTVVAQIQSAPLPDLLRAMLKYS
NNLMAEMIGMTASLQRGARPSSLKSSGQRMSDWARSALGMQHCKMVDHSGLGEESRLSANDMVRALLASRRTGALKPLLK
PYYFNDAKGRPIKTHPVKIAAKTGTLNFVSGLGGFINTPDGGELVFAIFSADQRARAKISRAERERPRGGRSWNTRAKKL
QQNLLDRWGQVYGS

Sequences:

>Translated_494_residues
MISRRHFLSTALAGLAAAPALANAPGYTPRPKARGLSPYTPGADGLDAILRGAGLSGQSTCVVANARTGQVLESVEGDAL
LPPASVAKVLTALYALDSLGAGYRFRTRLVATGPLKNGIIQGDLVLVGGADPTLDTDGLANLAGQMKTAGVRGVTGRLLI
WEDMLPYIRSIDPDQPDHIGYSPSVSGIALNFNRVHFEWKRAGNNWAITMDARTQKYRPDVYTSKMAVARRDLPVYSYEN
KAGIDHWTVASGALGNGGARWLPVRNPALYAGDVFQTLARANGIALPSPKTTATLPGGTVVAQIQSAPLPDLLRAMLKYS
NNLMAEMIGMTASLQRGARPSSLKSSGQRMSDWARSALGMQHCKMVDHSGLGEESRLSANDMVRALLASRRTGALKPLLK
PYYFNDAKGRPIKTHPVKIAAKTGTLNFVSGLGGFINTPDGGELVFAIFSADQRARAKISRAERERPRGGRSWNTRAKKL
QQNLLDRWGQVYGS
>Mature_494_residues
MISRRHFLSTALAGLAAAPALANAPGYTPRPKARGLSPYTPGADGLDAILRGAGLSGQSTCVVANARTGQVLESVEGDAL
LPPASVAKVLTALYALDSLGAGYRFRTRLVATGPLKNGIIQGDLVLVGGADPTLDTDGLANLAGQMKTAGVRGVTGRLLI
WEDMLPYIRSIDPDQPDHIGYSPSVSGIALNFNRVHFEWKRAGNNWAITMDARTQKYRPDVYTSKMAVARRDLPVYSYEN
KAGIDHWTVASGALGNGGARWLPVRNPALYAGDVFQTLARANGIALPSPKTTATLPGGTVVAQIQSAPLPDLLRAMLKYS
NNLMAEMIGMTASLQRGARPSSLKSSGQRMSDWARSALGMQHCKMVDHSGLGEESRLSANDMVRALLASRRTGALKPLLK
PYYFNDAKGRPIKTHPVKIAAKTGTLNFVSGLGGFINTPDGGELVFAIFSADQRARAKISRAERERPRGGRSWNTRAKKL
QQNLLDRWGQVYGS

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=420, Percent_Identity=28.0952380952381, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 52884; Mature: 52884

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRRHFLSTALAGLAAAPALANAPGYTPRPKARGLSPYTPGADGLDAILRGAGLSGQST
CCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCE
CVVANARTGQVLESVEGDALLPPASVAKVLTALYALDSLGAGYRFRTRLVATGPLKNGII
EEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEE
QGDLVLVGGADPTLDTDGLANLAGQMKTAGVRGVTGRLLIWEDMLPYIRSIDPDQPDHIG
ECCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCC
YSPSVSGIALNFNRVHFEWKRAGNNWAITMDARTQKYRPDVYTSKMAVARRDLPVYSYEN
CCCCCCEEEEEEEEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEECCC
KAGIDHWTVASGALGNGGARWLPVRNPALYAGDVFQTLARANGIALPSPKTTATLPGGTV
CCCCCEEEECCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCCCEEECCCCEE
VAQIQSAPLPDLLRAMLKYSNNLMAEMIGMTASLQRGARPSSLKSSGQRMSDWARSALGM
EEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHH
QHCKMVDHSGLGEESRLSANDMVRALLASRRTGALKPLLKPYYFNDAKGRPIKTHPVKIA
HHHHHHHCCCCCCHHHCCHHHHHHHHHHHCCCCCHHHHHCCCEECCCCCCCEEECCEEEE
AKTGTLNFVSGLGGFINTPDGGELVFAIFSADQRARAKISRAERERPRGGRSWNTRAKKL
EECCCCHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
QQNLLDRWGQVYGS
HHHHHHHHHHHHCC
>Mature Secondary Structure
MISRRHFLSTALAGLAAAPALANAPGYTPRPKARGLSPYTPGADGLDAILRGAGLSGQST
CCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCE
CVVANARTGQVLESVEGDALLPPASVAKVLTALYALDSLGAGYRFRTRLVATGPLKNGII
EEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEE
QGDLVLVGGADPTLDTDGLANLAGQMKTAGVRGVTGRLLIWEDMLPYIRSIDPDQPDHIG
ECCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCC
YSPSVSGIALNFNRVHFEWKRAGNNWAITMDARTQKYRPDVYTSKMAVARRDLPVYSYEN
CCCCCCEEEEEEEEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEECCC
KAGIDHWTVASGALGNGGARWLPVRNPALYAGDVFQTLARANGIALPSPKTTATLPGGTV
CCCCCEEEECCCCCCCCCEEEEECCCCCEEHHHHHHHHHHHCCCCCCCCCCEEECCCCEE
VAQIQSAPLPDLLRAMLKYSNNLMAEMIGMTASLQRGARPSSLKSSGQRMSDWARSALGM
EEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHH
QHCKMVDHSGLGEESRLSANDMVRALLASRRTGALKPLLKPYYFNDAKGRPIKTHPVKIA
HHHHHHHCCCCCCHHHCCHHHHHHHHHHHCCCCCHHHHHCCCEECCCCCCCEEECCEEEE
AKTGTLNFVSGLGGFINTPDGGELVFAIFSADQRARAKISRAERERPRGGRSWNTRAKKL
EECCCCHHHHHCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHH
QQNLLDRWGQVYGS
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2040429; 9278503; 2046551 [H]