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The map label for this gene is bclA [H]

Identifier: 99082078

GI number: 99082078

Start: 2351314

End: 2352930

Strand: Reverse

Name: bclA [H]

Synonym: TM1040_2238

Alternate gene names: 99082078

Gene position: 2352930-2351314 (Counterclockwise)

Preceding gene: 99082079

Following gene: 99082077

Centisome position: 73.51

GC content: 60.73

Gene sequence:

>1617_bases
ATGATGACGCTCGGAGCATCCGCACATACCGATACATTCACCCGCGACGCCTTGCCGCCTGCGGATCAGTGGCCCGATTT
TATGCTTGAGGGGTTTGAGTATCCCGAGCATCTGAACGCGGGCGTCGAACTGACCGATGCGATGGTGGAGCGCGGCTTTG
GCGACCGTACTGCACTGATCGGCAACGGGCGGCAGCGCACCTATAAGGAGTTGGCCGACTGGACCAACCGGCTGGCGCAT
GCGCTGGTTCAGGATCTGGGCGTCAAACCGGGCAATCGCATCCTGATCCGTTCTGCGAATAATCCGGCGATGGTGGCCTG
CTGGCTCGCCGCCACCAAGGCGGGCGCGGTGGTGGTCAACACCATGCCGATGCTGCGCGCTGCAGAGCTAAAAAAATATG
TCGAGAAGGCGCAGATCCAGTTTGCGCTCTGTGATACGCGGCTGATGGAGGAGATGGAGGCCTGCGCGGCCCAGTGCGAT
GTGCTTGAGCGTGTCGTGGGCTTTGATGGGACCTCCAACCACGACGCTGAACTTGACCGGCTGGCACTGGAGAAACCGGT
GCAGTTTGAGGCGGTAGAGACCGGACGCGATGATGTGGCGCTCCTGGGGTTCACCTCCGGGTCGACCGGAGAGCCCAAGG
CCACCATGCATTTTCACCGCGATCTATTGATCATTGCCGATGGCTATGCCCGTGATGTGCTCAATGTGGTGCCGGAGGAC
ATTTTTGTGGGATCTCCGCCACTTGCCTTTACCTTCGGGCTTGGCGGGCTAGCGATTTTCCCGTTACGGTTCGGGGCAAC
CGCGACGCTTTTGGAACAGGCCACACCGCCCAATATGATCGAGATCATCGAGAAATACCGCGCGACCGTGTGCTTTACTG
CGCCGACCGCCTATCGCGTGATGCTCTCGGCGATGGAAGAGGGCGCGGATCTGAGCAGCCTGCGCGCCGCTGTCTCTGCG
GGCGAGACCCTGCCCAAACCTGTCTATGACGATTGGATGGCCAAGACCGGCAAGCCGATGCTCGATGGGATCGGCGCGAC
CGAGATGCTGCATATCTTTATCTCCAACCGGTTTGACGACCACCGTCCTGCCTGCACCGGCAAACCTGTGCGCGGCTACG
AGGCCAAGGTGGTCGATGACGAAGGCAACACTGTGCCCGATGGTGAAGTTGGCCGCCTTGCGGTGCGCGGGCCGACGGGC
TGTCGCTACCTTAATGATGATCGGCAGGCGGGCTATGTCGCGGATGGTTGGAACATCACCGGCGACAGTTTCATCCGCGA
TGCGGACGGCTATTTCCATTTTGCTGCGCGCAACGACGATATGATCGTCTCGGCGGGCTACAATATCGCTGGCCCCGAGG
TGGAAGCTGCGCTTCTGGCGCATGCGGCGGTGGCGGAATGTGCGGTCATTGGCGCCCCGGACGAGGCGCGCGGACATATC
GTGCAGGCGCATATCGTGCTCAACGCGGACTTCGCGCCGTCTGAGACGCTCATCAAGGATCTGCAGAGCCACGTGAAGGC
GACCATTGCGCCCTATAAATATCCCCGCTCGATCATTTTTGCCGAAGAACTGCCAAAGACGCAGACCGGCAAGATTCAGC
GGTTCCGGCTTCGTTGA

Upstream 100 bases:

>100_bases
AACTCTATCGCGAAATCAGGGCGTTGCGCATCTATGAGGGCGCGAGCGACGTTCAGCGCGTGGTGATCGCGCGGCAGGCG
ATGATGGCATTGGAGGGCAA

Downstream 100 bases:

>100_bases
ATAATAAAACAAACCAACCACAAACAGGGAGTTTGATCATGAAGAAGCTAATGTTGGCCACAGCGGCCGCGGCGCTGGCC
GCGGGCGGAGCGATGGCAGA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Benzoyl-CoA synthetase [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MMTLGASAHTDTFTRDALPPADQWPDFMLEGFEYPEHLNAGVELTDAMVERGFGDRTALIGNGRQRTYKELADWTNRLAH
ALVQDLGVKPGNRILIRSANNPAMVACWLAATKAGAVVVNTMPMLRAAELKKYVEKAQIQFALCDTRLMEEMEACAAQCD
VLERVVGFDGTSNHDAELDRLALEKPVQFEAVETGRDDVALLGFTSGSTGEPKATMHFHRDLLIIADGYARDVLNVVPED
IFVGSPPLAFTFGLGGLAIFPLRFGATATLLEQATPPNMIEIIEKYRATVCFTAPTAYRVMLSAMEEGADLSSLRAAVSA
GETLPKPVYDDWMAKTGKPMLDGIGATEMLHIFISNRFDDHRPACTGKPVRGYEAKVVDDEGNTVPDGEVGRLAVRGPTG
CRYLNDDRQAGYVADGWNITGDSFIRDADGYFHFAARNDDMIVSAGYNIAGPEVEAALLAHAAVAECAVIGAPDEARGHI
VQAHIVLNADFAPSETLIKDLQSHVKATIAPYKYPRSIIFAEELPKTQTGKIQRFRLR

Sequences:

>Translated_538_residues
MMTLGASAHTDTFTRDALPPADQWPDFMLEGFEYPEHLNAGVELTDAMVERGFGDRTALIGNGRQRTYKELADWTNRLAH
ALVQDLGVKPGNRILIRSANNPAMVACWLAATKAGAVVVNTMPMLRAAELKKYVEKAQIQFALCDTRLMEEMEACAAQCD
VLERVVGFDGTSNHDAELDRLALEKPVQFEAVETGRDDVALLGFTSGSTGEPKATMHFHRDLLIIADGYARDVLNVVPED
IFVGSPPLAFTFGLGGLAIFPLRFGATATLLEQATPPNMIEIIEKYRATVCFTAPTAYRVMLSAMEEGADLSSLRAAVSA
GETLPKPVYDDWMAKTGKPMLDGIGATEMLHIFISNRFDDHRPACTGKPVRGYEAKVVDDEGNTVPDGEVGRLAVRGPTG
CRYLNDDRQAGYVADGWNITGDSFIRDADGYFHFAARNDDMIVSAGYNIAGPEVEAALLAHAAVAECAVIGAPDEARGHI
VQAHIVLNADFAPSETLIKDLQSHVKATIAPYKYPRSIIFAEELPKTQTGKIQRFRLR
>Mature_538_residues
MMTLGASAHTDTFTRDALPPADQWPDFMLEGFEYPEHLNAGVELTDAMVERGFGDRTALIGNGRQRTYKELADWTNRLAH
ALVQDLGVKPGNRILIRSANNPAMVACWLAATKAGAVVVNTMPMLRAAELKKYVEKAQIQFALCDTRLMEEMEACAAQCD
VLERVVGFDGTSNHDAELDRLALEKPVQFEAVETGRDDVALLGFTSGSTGEPKATMHFHRDLLIIADGYARDVLNVVPED
IFVGSPPLAFTFGLGGLAIFPLRFGATATLLEQATPPNMIEIIEKYRATVCFTAPTAYRVMLSAMEEGADLSSLRAAVSA
GETLPKPVYDDWMAKTGKPMLDGIGATEMLHIFISNRFDDHRPACTGKPVRGYEAKVVDDEGNTVPDGEVGRLAVRGPTG
CRYLNDDRQAGYVADGWNITGDSFIRDADGYFHFAARNDDMIVSAGYNIAGPEVEAALLAHAAVAECAVIGAPDEARGHI
VQAHIVLNADFAPSETLIKDLQSHVKATIAPYKYPRSIIFAEELPKTQTGKIQRFRLR

Specific function: Catalyzes the ligation of benzoate and CoA to form benzoyl-CoA at the expense of ATP. The enzyme also ligates 2- aminobenzoate and CoA. The enzyme shows activity toward a number of benzoate derivates [H]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. Benzoate-CoA ligase subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544132, Length=490, Percent_Identity=30.6122448979592, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI122937307, Length=535, Percent_Identity=28.9719626168224, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI38505220, Length=485, Percent_Identity=30.7216494845361, Blast_Score=218, Evalue=9e-57,
Organism=Homo sapiens, GI58082049, Length=493, Percent_Identity=29.8174442190669, Blast_Score=215, Evalue=8e-56,
Organism=Homo sapiens, GI157311624, Length=493, Percent_Identity=29.6146044624746, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI157311622, Length=493, Percent_Identity=29.6146044624746, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI115511026, Length=542, Percent_Identity=27.6752767527675, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI187761345, Length=528, Percent_Identity=26.7045454545455, Blast_Score=166, Evalue=8e-41,
Organism=Homo sapiens, GI187761343, Length=528, Percent_Identity=26.7045454545455, Blast_Score=166, Evalue=8e-41,
Organism=Homo sapiens, GI28416953, Length=548, Percent_Identity=29.7445255474453, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI13375727, Length=582, Percent_Identity=23.8831615120275, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI8923896, Length=570, Percent_Identity=24.5614035087719, Blast_Score=130, Evalue=4e-30,
Organism=Homo sapiens, GI253970489, Length=374, Percent_Identity=28.8770053475936, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI156151445, Length=524, Percent_Identity=25.381679389313, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI195947378, Length=458, Percent_Identity=21.8340611353712, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI42544134, Length=321, Percent_Identity=22.4299065420561, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI145693145, Length=503, Percent_Identity=31.2127236580517, Blast_Score=181, Evalue=8e-47,
Organism=Escherichia coli, GI1790505, Length=538, Percent_Identity=28.4386617100372, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1788107, Length=540, Percent_Identity=27.037037037037, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI221142682, Length=507, Percent_Identity=26.4299802761341, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI1786810, Length=544, Percent_Identity=26.6544117647059, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1786529, Length=531, Percent_Identity=26.5536723163842, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789201, Length=354, Percent_Identity=24.8587570621469, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=542, Percent_Identity=28.5977859778598, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=542, Percent_Identity=28.5977859778598, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71994690, Length=542, Percent_Identity=28.5977859778598, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17538037, Length=500, Percent_Identity=26.4, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17557194, Length=501, Percent_Identity=26.3473053892216, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17558820, Length=498, Percent_Identity=25.3012048192771, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71996755, Length=513, Percent_Identity=25.3411306042885, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71982997, Length=562, Percent_Identity=25.6227758007117, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI71983001, Length=562, Percent_Identity=25.6227758007117, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32563687, Length=486, Percent_Identity=24.0740740740741, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=491, Percent_Identity=23.8289205702648, Blast_Score=112, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17560140, Length=497, Percent_Identity=23.943661971831, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=364, Percent_Identity=27.4725274725275, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17560308, Length=515, Percent_Identity=25.2427184466019, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=396, Percent_Identity=27.020202020202, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319264, Length=527, Percent_Identity=28.0834914611006, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6323182, Length=545, Percent_Identity=26.9724770642202, Blast_Score=143, Evalue=6e-35,
Organism=Saccharomyces cerevisiae, GI6319699, Length=359, Percent_Identity=27.5766016713092, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24667955, Length=558, Percent_Identity=28.3154121863799, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI24581924, Length=522, Percent_Identity=26.0536398467433, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI62472339, Length=526, Percent_Identity=28.3269961977186, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24667959, Length=438, Percent_Identity=29.4520547945205, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21355181, Length=511, Percent_Identity=27.9843444227006, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI21355225, Length=555, Percent_Identity=24.6846846846847, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI18859661, Length=495, Percent_Identity=23.6363636363636, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24656500, Length=514, Percent_Identity=22.1789883268482, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653035, Length=492, Percent_Identity=23.5772357723577, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922652, Length=499, Percent_Identity=22.0440881763527, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24648253, Length=479, Percent_Identity=22.3382045929019, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648255, Length=479, Percent_Identity=22.3382045929019, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21356441, Length=410, Percent_Identity=23.1707317073171, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356947, Length=496, Percent_Identity=23.3870967741935, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI281365686, Length=309, Percent_Identity=24.2718446601942, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR011957 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.25 [H]

Molecular weight: Translated: 58622; Mature: 58622

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLGASAHTDTFTRDALPPADQWPDFMLEGFEYPEHLNAGVELTDAMVERGFGDRTALI
CCCCCCCCCCCCCHHCCCCCCCCCCHHHHHCCCCHHHCCCCCHHHHHHHHCCCCCCEEEE
GNGRQRTYKELADWTNRLAHALVQDLGVKPGNRILIRSANNPAMVACWLAATKAGAVVVN
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEHHCCCCEEEEE
TMPMLRAAELKKYVEKAQIQFALCDTRLMEEMEACAAQCDVLERVVGFDGTSNHDAELDR
CCHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LALEKPVQFEAVETGRDDVALLGFTSGSTGEPKATMHFHRDLLIIADGYARDVLNVVPED
HHHCCCCEEEEECCCCCCEEEEEECCCCCCCCCEEEEECCEEEEEECCHHHHHHHHCCHH
IFVGSPPLAFTFGLGGLAIFPLRFGATATLLEQATPPNMIEIIEKYRATVCFTAPTAYRV
HCCCCCCCEEEECCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHCCEEEEECCHHHHH
MLSAMEEGADLSSLRAAVSAGETLPKPVYDDWMAKTGKPMLDGIGATEMLHIFISNRFDD
HHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCC
HRPACTGKPVRGYEAKVVDDEGNTVPDGEVGRLAVRGPTGCRYLNDDRQAGYVADGWNIT
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCC
GDSFIRDADGYFHFAARNDDMIVSAGYNIAGPEVEAALLAHAAVAECAVIGAPDEARGHI
CCHHEECCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCE
VQAHIVLNADFAPSETLIKDLQSHVKATIAPYKYPRSIIFAEELPKTQTGKIQRFRLR
EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCCCEEEECC
>Mature Secondary Structure
MMTLGASAHTDTFTRDALPPADQWPDFMLEGFEYPEHLNAGVELTDAMVERGFGDRTALI
CCCCCCCCCCCCCHHCCCCCCCCCCHHHHHCCCCHHHCCCCCHHHHHHHHCCCCCCEEEE
GNGRQRTYKELADWTNRLAHALVQDLGVKPGNRILIRSANNPAMVACWLAATKAGAVVVN
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEEHHCCCCEEEEE
TMPMLRAAELKKYVEKAQIQFALCDTRLMEEMEACAAQCDVLERVVGFDGTSNHDAELDR
CCHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
LALEKPVQFEAVETGRDDVALLGFTSGSTGEPKATMHFHRDLLIIADGYARDVLNVVPED
HHHCCCCEEEEECCCCCCEEEEEECCCCCCCCCEEEEECCEEEEEECCHHHHHHHHCCHH
IFVGSPPLAFTFGLGGLAIFPLRFGATATLLEQATPPNMIEIIEKYRATVCFTAPTAYRV
HCCCCCCCEEEECCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHCCEEEEECCHHHHH
MLSAMEEGADLSSLRAAVSAGETLPKPVYDDWMAKTGKPMLDGIGATEMLHIFISNRFDD
HHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCC
HRPACTGKPVRGYEAKVVDDEGNTVPDGEVGRLAVRGPTGCRYLNDDRQAGYVADGWNIT
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCC
GDSFIRDADGYFHFAARNDDMIVSAGYNIAGPEVEAALLAHAAVAECAVIGAPDEARGHI
CCHHEECCCCEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCCE
VQAHIVLNADFAPSETLIKDLQSHVKATIAPYKYPRSIIFAEELPKTQTGKIQRFRLR
EEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12897012 [H]