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The map label for this gene is braD [H]

Identifier: 99082076

GI number: 99082076

Start: 2349167

End: 2350087

Strand: Reverse

Name: braD [H]

Synonym: TM1040_2236

Alternate gene names: 99082076

Gene position: 2350087-2349167 (Counterclockwise)

Preceding gene: 99082077

Following gene: 99082075

Centisome position: 73.42

GC content: 61.56

Gene sequence:

>921_bases
ATGTCCACGATACTTATCATCGAGCAGGTGCTGAACGGGCTTCAGTCCGGCGTCATGCTCTTTCTCATGGCGGCGGGGCT
GACGCTGATTTTTGGGGTCATGGGCCTCATCAATCTTGCCCATGGCTCGCTCTATATGGTTGGGGCCTTTGCCGCGGCGG
CCGTGGCGGGCGTCACGGGGTCCTTCGTGCTGGCGCTTATCGCGGCGCTTGCGGCGGCGGCGCTTGCGGGGGCTGTGATC
GAGGTTGCGGTGATCCGCAGGCTCTATGACAGCGATCACCTCGATCAGGTGCTGGCGACCTTTGCGCTCATTCTGATTTT
CTCCGAAGGCACGCGCTGGGTTTTTGGCTCTTTTCCGCTGTTTCTGGACATTCCCGATGCCTTGTCGGGGCCGGTTACCC
TGCCCGGAGGTATCCAGTATCCGCTTTATCGTCTTGCGCTCATCGGGGTGGGGCTTCTGGTGGCCTTCGGCCTGTGGCTC
CTGATCGAGCGCACCCGGATCGGCATTCAGATCCGTGCGGGCGAGAACGACCGAGAGATGATTGCCGCCCTGGGTGTGGA
CATCTCCAAGCTCTATACGCTGGTCTTTGCCCTTGGCGCCGCGTTGGCCGGTCTTGCGGGGGCGCTGGTTGGTGCGATCC
AGTCGGTGCAGGTCGGCATGGGCGAGCCGGTGCTGATCCTTGCCTTCGTCGTGATCGTGATCGGCGGTATCGGATCCATC
AAAGGGGCCTTTATCGGCGCATTGCTGGTGGGGCTCACGGATACATTGGTCGGGATCTTCCTGCCCGAAGTGCTCAAACT
CTTTATGGACGCGGCCCCCGCAACATCTGCCGGATCGTCGATCGCCTCGATGTCGATCTACATCCTGATGAGCCTTGTAC
TGGTCTGGCGTCCCTCTGGCCTGTTCGGAGCCCGCGCATGA

Upstream 100 bases:

>100_bases
TGAGGATCATGCAAACGCCTATGTGGACGAGTGCAAGATGTAAGTGAGGCGCGCCCGGCTCGCAGTCTGTGCGGGCCGGG
ACACACCTGAAAGAAGACAC

Downstream 100 bases:

>100_bases
CACGAGAAACCTATTTTAACGCAGCCCTGATGCTGGGCCTCATTGCCGTGCCTGCATGGGCGCTGATGAGCGATGAACCT
TTCACCATCACGCTTGCGAC

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSTILIIEQVLNGLQSGVMLFLMAAGLTLIFGVMGLINLAHGSLYMVGAFAAAAVAGVTGSFVLALIAALAAAALAGAVI
EVAVIRRLYDSDHLDQVLATFALILIFSEGTRWVFGSFPLFLDIPDALSGPVTLPGGIQYPLYRLALIGVGLLVAFGLWL
LIERTRIGIQIRAGENDREMIAALGVDISKLYTLVFALGAALAGLAGALVGAIQSVQVGMGEPVLILAFVVIVIGGIGSI
KGAFIGALLVGLTDTLVGIFLPEVLKLFMDAAPATSAGSSIASMSIYILMSLVLVWRPSGLFGARA

Sequences:

>Translated_306_residues
MSTILIIEQVLNGLQSGVMLFLMAAGLTLIFGVMGLINLAHGSLYMVGAFAAAAVAGVTGSFVLALIAALAAAALAGAVI
EVAVIRRLYDSDHLDQVLATFALILIFSEGTRWVFGSFPLFLDIPDALSGPVTLPGGIQYPLYRLALIGVGLLVAFGLWL
LIERTRIGIQIRAGENDREMIAALGVDISKLYTLVFALGAALAGLAGALVGAIQSVQVGMGEPVLILAFVVIVIGGIGSI
KGAFIGALLVGLTDTLVGIFLPEVLKLFMDAAPATSAGSSIASMSIYILMSLVLVWRPSGLFGARA
>Mature_305_residues
STILIIEQVLNGLQSGVMLFLMAAGLTLIFGVMGLINLAHGSLYMVGAFAAAAVAGVTGSFVLALIAALAAAALAGAVIE
VAVIRRLYDSDHLDQVLATFALILIFSEGTRWVFGSFPLFLDIPDALSGPVTLPGGIQYPLYRLALIGVGLLVAFGLWLL
IERTRIGIQIRAGENDREMIAALGVDISKLYTLVFALGAALAGLAGALVGAIQSVQVGMGEPVLILAFVVIVIGGIGSIK
GAFIGALLVGLTDTLVGIFLPEVLKLFMDAAPATSAGSSIASMSIYILMSLVLVWRPSGLFGARA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=287, Percent_Identity=27.8745644599303, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 31584; Mature: 31453

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTILIIEQVLNGLQSGVMLFLMAAGLTLIFGVMGLINLAHGSLYMVGAFAAAAVAGVTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SFVLALIAALAAAALAGAVIEVAVIRRLYDSDHLDQVLATFALILIFSEGTRWVFGSFPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCE
FLDIPDALSGPVTLPGGIQYPLYRLALIGVGLLVAFGLWLLIERTRIGIQIRAGENDREM
EEECCHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHH
IAALGVDISKLYTLVFALGAALAGLAGALVGAIQSVQVGMGEPVLILAFVVIVIGGIGSI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHH
KGAFIGALLVGLTDTLVGIFLPEVLKLFMDAAPATSAGSSIASMSIYILMSLVLVWRPSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
LFGARA
CCCCCC
>Mature Secondary Structure 
STILIIEQVLNGLQSGVMLFLMAAGLTLIFGVMGLINLAHGSLYMVGAFAAAAVAGVTG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
SFVLALIAALAAAALAGAVIEVAVIRRLYDSDHLDQVLATFALILIFSEGTRWVFGSFPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEECCCE
FLDIPDALSGPVTLPGGIQYPLYRLALIGVGLLVAFGLWLLIERTRIGIQIRAGENDREM
EEECCHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHH
IAALGVDISKLYTLVFALGAALAGLAGALVGAIQSVQVGMGEPVLILAFVVIVIGGIGSI
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHH
KGAFIGALLVGLTDTLVGIFLPEVLKLFMDAAPATSAGSSIASMSIYILMSLVLVWRPSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
LFGARA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]