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The map label for this gene is gloB [H]

Identifier: 99081931

GI number: 99081931

Start: 2194905

End: 2195720

Strand: Direct

Name: gloB [H]

Synonym: TM1040_2091

Alternate gene names: 99081931

Gene position: 2194905-2195720 (Clockwise)

Preceding gene: 99081922

Following gene: 99081932

Centisome position: 68.57

GC content: 59.56

Gene sequence:

>816_bases
ATGACCATGCCCGATCAGACCTCTTTCCCCTCCACCGTGGCAAATGGCCTCGAAATCGTGACGGTTCCGTGTCTTGCGGA
CAACTATGCCTACCTCGTCAAAGGACCCGATGGCGTGGCGCTGATCGATGCGCCCGAGGCCGAGCCGATCCTGACCGCTC
TGGAGGCGCGGGACTGGTCGCTTGGGGTCATCATGATTACCCATCACCACCACGACCATGTGGGCGGTGTCGCCGCACTG
CGTGAAAAATTCGGCTGCCCGGTCATGGGGCCCAAGGCCGAAGCCGATAAGATCGCAGCCCAGGGGGTCACACTGGATAT
GGAACTGGAGCCCGGCTTTGAAGGCGGCACCGGTGCCGGGCGCTGCCAGGTGCTTGCAGTGCCGGGCCACACGCTCGGCC
ACATCGCTTTTTATTATCCCGAGGCGCCGGCCCTGTTCAGCGCCGACAGCCTCATGGTGATGGGCTGTGGACGCCTTTTT
GAGGGCACGCCCGCGATGATGTGGCAGACCATGACCACGCTTTCTGCGCTCCCTGACGAGACGCTGATCTATTCCGGTCA
CGAATACACCGAGAGCAACGCAGGCTTTGCGCTCAGTCTGGAGCCGGAAAATGCAGCACTGCAAAAAAGAATGGATGAAA
TCACCAAAACCCGTCAAAACGGCCAACCGACCGTCCCTGCCCTTCTGTCGCTCGAAAAACAGACCAACCCGTTCCTGCGA
GCTGGCGATCCAAACCTGAAGAAAACGCTTGGTTTGGAAGGGGTTGAAGACGCAGAGGTCTTTGCCCATATCCGTGTGGC
GAAGGATAAGTTCTGA

Upstream 100 bases:

>100_bases
TGCTGCGGTAATAGAAATTCCTCAGATCCTGCACATCCAGATGCATTGCAGACGCTCACTGCTTCGGTCACTCTGGGGCA
GTCTAGCAATGTGAAAGCGA

Downstream 100 bases:

>100_bases
CACCAGTTTGTGGTCAGATGTGCATCGCCACACAAGATATGTGAACACAATCTCGGGAATTGTGACCACAATCGCGCAAA
AAGCCCTTGAAGCTTACGAT

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTMPDQTSFPSTVANGLEIVTVPCLADNYAYLVKGPDGVALIDAPEAEPILTALEARDWSLGVIMITHHHHDHVGGVAAL
REKFGCPVMGPKAEADKIAAQGVTLDMELEPGFEGGTGAGRCQVLAVPGHTLGHIAFYYPEAPALFSADSLMVMGCGRLF
EGTPAMMWQTMTTLSALPDETLIYSGHEYTESNAGFALSLEPENAALQKRMDEITKTRQNGQPTVPALLSLEKQTNPFLR
AGDPNLKKTLGLEGVEDAEVFAHIRVAKDKF

Sequences:

>Translated_271_residues
MTMPDQTSFPSTVANGLEIVTVPCLADNYAYLVKGPDGVALIDAPEAEPILTALEARDWSLGVIMITHHHHDHVGGVAAL
REKFGCPVMGPKAEADKIAAQGVTLDMELEPGFEGGTGAGRCQVLAVPGHTLGHIAFYYPEAPALFSADSLMVMGCGRLF
EGTPAMMWQTMTTLSALPDETLIYSGHEYTESNAGFALSLEPENAALQKRMDEITKTRQNGQPTVPALLSLEKQTNPFLR
AGDPNLKKTLGLEGVEDAEVFAHIRVAKDKF
>Mature_270_residues
TMPDQTSFPSTVANGLEIVTVPCLADNYAYLVKGPDGVALIDAPEAEPILTALEARDWSLGVIMITHHHHDHVGGVAALR
EKFGCPVMGPKAEADKIAAQGVTLDMELEPGFEGGTGAGRCQVLAVPGHTLGHIAFYYPEAPALFSADSLMVMGCGRLFE
GTPAMMWQTMTTLSALPDETLIYSGHEYTESNAGFALSLEPENAALQKRMDEITKTRQNGQPTVPALLSLEKQTNPFLRA
GDPNLKKTLGLEGVEDAEVFAHIRVAKDKF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI116642887, Length=247, Percent_Identity=33.6032388663968, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI21703352, Length=247, Percent_Identity=33.6032388663968, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI14150041, Length=244, Percent_Identity=35.655737704918, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI94538320, Length=264, Percent_Identity=32.5757575757576, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI94538322, Length=270, Percent_Identity=32.962962962963, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI46361987, Length=205, Percent_Identity=35.609756097561, Blast_Score=108, Evalue=4e-24,
Organism=Escherichia coli, GI1786406, Length=259, Percent_Identity=41.3127413127413, Blast_Score=170, Evalue=9e-44,
Organism=Escherichia coli, GI1787158, Length=135, Percent_Identity=35.5555555555556, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17536925, Length=260, Percent_Identity=31.9230769230769, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320478, Length=267, Percent_Identity=27.7153558052434, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324614, Length=246, Percent_Identity=28.8617886178862, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21356335, Length=265, Percent_Identity=34.7169811320755, Blast_Score=151, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24667711, Length=265, Percent_Identity=34.7169811320755, Blast_Score=150, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24667703, Length=265, Percent_Identity=34.7169811320755, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI221330176, Length=202, Percent_Identity=27.2277227722772, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29091; Mature: 28959

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMPDQTSFPSTVANGLEIVTVPCLADNYAYLVKGPDGVALIDAPEAEPILTALEARDWS
CCCCCCCCCCHHHHCCEEEEEEEEECCCCEEEEECCCCEEEEECCCCCHHHHHHHCCCCE
LGVIMITHHHHDHVGGVAALREKFGCPVMGPKAEADKIAAQGVTLDMELEPGFEGGTGAG
EEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCC
RCQVLAVPGHTLGHIAFYYPEAPALFSADSLMVMGCGRLFEGTPAMMWQTMTTLSALPDE
EEEEEEECCCCCCEEEEEECCCCCEECCCCEEEEECCHHHCCCHHHHHHHHHHHHHCCCC
TLIYSGHEYTESNAGFALSLEPENAALQKRMDEITKTRQNGQPTVPALLSLEKQTNPFLR
EEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCEEE
AGDPNLKKTLGLEGVEDAEVFAHIRVAKDKF
CCCCCHHHHHCCCCCCHHHHEEEEEEECCCC
>Mature Secondary Structure 
TMPDQTSFPSTVANGLEIVTVPCLADNYAYLVKGPDGVALIDAPEAEPILTALEARDWS
CCCCCCCCCHHHHCCEEEEEEEEECCCCEEEEECCCCEEEEECCCCCHHHHHHHCCCCE
LGVIMITHHHHDHVGGVAALREKFGCPVMGPKAEADKIAAQGVTLDMELEPGFEGGTGAG
EEEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCCC
RCQVLAVPGHTLGHIAFYYPEAPALFSADSLMVMGCGRLFEGTPAMMWQTMTTLSALPDE
EEEEEEECCCCCCEEEEEECCCCCEECCCCEEEEECCHHHCCCHHHHHHHHHHHHHCCCC
TLIYSGHEYTESNAGFALSLEPENAALQKRMDEITKTRQNGQPTVPALLSLEKQTNPFLR
EEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCHHHEEECCCCCCEEE
AGDPNLKKTLGLEGVEDAEVFAHIRVAKDKF
CCCCCHHHHHCCCCCCHHHHEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA