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The map label for this gene is queC

Identifier: 99081887

GI number: 99081887

Start: 2151940

End: 2152641

Strand: Reverse

Name: queC

Synonym: TM1040_2047

Alternate gene names: 99081887

Gene position: 2152641-2151940 (Counterclockwise)

Preceding gene: 99081893

Following gene: 99081886

Centisome position: 67.25

GC content: 63.25

Gene sequence:

>702_bases
ATGAAAACACTTGTCATCTGCTCTGGCGGACTCGATTCCGTGTCGCTGGCGCATATCACCTCCCAGAAATACCAACTTAC
CCGTCTGGTTTCCTTTGACTATGGTCAACGCCATCGCAAGGAACTTGACTTTGCCCGCGCCGCTGCCACGCGGCTGGGCG
TGCCGTTTCACCTGATCGACATGCGCAGCATCGGTGGCGCGCTCTCCGGGTCGGCGCTCACCGACGACATCGACGTCCCC
GATGGGCATTACGCCGAGGACACCATGCGGATCACCGTGGTGCCCAACCGCAATGCGATCATGCTTGCGATTGGCTTTGG
CGTCGCGGCGGCGCAAGGGGATGAGGCCGTGGCCACCGCCGTGCATGGCGGGGATCACTTCATCTATCCCGATTGTCGAC
CCGGCTTCACCCGCGCCTTTGAAACGATGCAGCGCGCGGCGCTGGACGGTTATGCCGATGTGCGCCTGCACACGCCCTTT
GTCGAGCAGAGCAAGGCCGACATCGTGCGTGCCGGCGCCGCTCACAACACGCCCTTTGCTGAAACATGGTCCTGCTACAA
GGGCGGTGACATCCACTGCGGCCGTTGTGGCACCTGCGTGGAGCGGCGCGAGGCTTTCCATCTGGCGGAGGTTGCGGACC
CCACGGTCTATGCCGACCCAGACTATTGGAGCGCGGCCATTGCCGGGCGGGAGGCAGAGTGA

Upstream 100 bases:

>100_bases
AAGCGCCCAGAGAGAGGGCTCTTGCAAATTCCGCGGTTTCTGAAATAAGGCCCGCCATCCGGTCGCAGCCTACCCGGACA
TCAAAACAAGGGCCCCAGAT

Downstream 100 bases:

>100_bases
TGTTTCGCATTCGCAAGGAGTTTCACTTCTCCGCCTCGCACCAGTTGTCACATCTGCCTTTGGATCACCAATGCGCGCGC
CTGCATGGGCACAACTATAT

Product: ExsB

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MKTLVICSGGLDSVSLAHITSQKYQLTRLVSFDYGQRHRKELDFARAAATRLGVPFHLIDMRSIGGALSGSALTDDIDVP
DGHYAEDTMRITVVPNRNAIMLAIGFGVAAAQGDEAVATAVHGGDHFIYPDCRPGFTRAFETMQRAALDGYADVRLHTPF
VEQSKADIVRAGAAHNTPFAETWSCYKGGDIHCGRCGTCVERREAFHLAEVADPTVYADPDYWSAAIAGREAE

Sequences:

>Translated_233_residues
MKTLVICSGGLDSVSLAHITSQKYQLTRLVSFDYGQRHRKELDFARAAATRLGVPFHLIDMRSIGGALSGSALTDDIDVP
DGHYAEDTMRITVVPNRNAIMLAIGFGVAAAQGDEAVATAVHGGDHFIYPDCRPGFTRAFETMQRAALDGYADVRLHTPF
VEQSKADIVRAGAAHNTPFAETWSCYKGGDIHCGRCGTCVERREAFHLAEVADPTVYADPDYWSAAIAGREAE
>Mature_233_residues
MKTLVICSGGLDSVSLAHITSQKYQLTRLVSFDYGQRHRKELDFARAAATRLGVPFHLIDMRSIGGALSGSALTDDIDVP
DGHYAEDTMRITVVPNRNAIMLAIGFGVAAAQGDEAVATAVHGGDHFIYPDCRPGFTRAFETMQRAALDGYADVRLHTPF
VEQSKADIVRAGAAHNTPFAETWSCYKGGDIHCGRCGTCVERREAFHLAEVADPTVYADPDYWSAAIAGREAE

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family

Homologues:

Organism=Escherichia coli, GI1786648, Length=232, Percent_Identity=34.9137931034483, Blast_Score=104, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEC_SILST (Q1GEY7)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_614041.1
- ProteinModelPortal:   Q1GEY7
- SMR:   Q1GEY7
- STRING:   Q1GEY7
- GeneID:   4077974
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_2047
- NMPDR:   fig|292414.1.peg.419
- eggNOG:   COG0603
- HOGENOM:   HBG553284
- OMA:   GWAEVLG
- PhylomeDB:   Q1GEY7
- ProtClustDB:   CLSK864390
- BioCyc:   SSP292414:TM1040_2047-MONOMER
- HAMAP:   MF_01633_B
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006293
- TIGRFAMs:   TIGR00364

Pfam domain/function: PF06508 ExsB

EC number: NA

Molecular weight: Translated: 25249; Mature: 25249

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLVICSGGLDSVSLAHITSQKYQLTRLVSFDYGQRHRKELDFARAAATRLGVPFHLID
CCEEEEECCCCCCEEEEEHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEE
MRSIGGALSGSALTDDIDVPDGHYAEDTMRITVVPNRNAIMLAIGFGVAAAQGDEAVATA
HHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCEEECCCCCEEEEE
VHGGDHFIYPDCRPGFTRAFETMQRAALDGYADVRLHTPFVEQSKADIVRAGAAHNTPFA
EECCCEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHCCCHHHEECCCCCCCCCH
ETWSCYKGGDIHCGRCGTCVERREAFHLAEVADPTVYADPDYWSAAIAGREAE
HCCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCCCCCC
>Mature Secondary Structure
MKTLVICSGGLDSVSLAHITSQKYQLTRLVSFDYGQRHRKELDFARAAATRLGVPFHLID
CCEEEEECCCCCCEEEEEHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEE
MRSIGGALSGSALTDDIDVPDGHYAEDTMRITVVPNRNAIMLAIGFGVAAAQGDEAVATA
HHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCEEECCCCCEEEEE
VHGGDHFIYPDCRPGFTRAFETMQRAALDGYADVRLHTPFVEQSKADIVRAGAAHNTPFA
EECCCEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHCCCHHHEECCCCCCCCCH
ETWSCYKGGDIHCGRCGTCVERREAFHLAEVADPTVYADPDYWSAAIAGREAE
HCCCCCCCCCEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA