Click here to switch to the map view.

The map label for this gene is hisH [H]

Identifier: 99081874

GI number: 99081874

Start: 2142831

End: 2143469

Strand: Direct

Name: hisH [H]

Synonym: TM1040_2034

Alternate gene names: 99081874

Gene position: 2142831-2143469 (Clockwise)

Preceding gene: 99081873

Following gene: 99081876

Centisome position: 66.94

GC content: 61.5

Gene sequence:

>639_bases
ATGCTGACCGCCATCATCGACTATGAGTCCGGCAATCTGCACTCCGCCGAGAAGGCCTTTCAGCGCATGGCGACAGAGGT
CGGCGCGGGGGATGTGGTTGTCACATCCGACGCCGATGTGGTGGCCCGCGCTGATCGGTTGGTGCTGCCCGGCGACGGTG
CCTTTCCCGCCTGCGCCGCAGAGCTGCGCGGCCACAAGGGCATCTATGACGCCATGGTCGAGGCTGTGGAGGTCAAGGGC
CGTCCCTTCCTCGGGATCTGCGTTGGCATGCAGTTGATGGCCACCACCGGTCACGAATATGAGGACACGCCGGGGCTTGG
CTGGGTCGACGGGGATGTGGTGAAAATCACCCCGGCAGAGGCACATCTGAAGGTGCCGCATATGGGCTGGAACGATCTGG
TGATCAATCACCCGCACCCGGTGTTTGACGGCATCTCGACCGGGGATCACTGCTATTTCGTGCACAGCTACCATTTCCGC
GTCGCCAACCCGAACGAACGGCTGGCACATGTGGATTATGCGGGCGATGTCACGGCAGTGATCGGGCGCGACACCATGCT
TGGCATGCAGTTTCACCCCGAAAAGAGCCAGCATGTCGGGCTCAAGATGATCGGCAATTTCCTCACCTGGAAACCCTGA

Upstream 100 bases:

>100_bases
CCAGAAGAGCCCCCAGGCTCTCGCCCGCTTTTGCCTCGCCCAGACGCGTGCCCTAAGAGGGCTGCCGGGCCGGGGCGCAT
AAGATCCCGAGGATTTACCT

Downstream 100 bases:

>100_bases
CCCCTGCCCTTATCCTGCTTTGTCTCCCGCTCTAATCTGGACGCAAGCCGCCCCTTGGGGCGGCACGCGCAAGAGGCGAC
AGGCTGCTTGCGCGCCACCG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLTAIIDYESGNLHSAEKAFQRMATEVGAGDVVVTSDADVVARADRLVLPGDGAFPACAAELRGHKGIYDAMVEAVEVKG
RPFLGICVGMQLMATTGHEYEDTPGLGWVDGDVVKITPAEAHLKVPHMGWNDLVINHPHPVFDGISTGDHCYFVHSYHFR
VANPNERLAHVDYAGDVTAVIGRDTMLGMQFHPEKSQHVGLKMIGNFLTWKP

Sequences:

>Translated_212_residues
MLTAIIDYESGNLHSAEKAFQRMATEVGAGDVVVTSDADVVARADRLVLPGDGAFPACAAELRGHKGIYDAMVEAVEVKG
RPFLGICVGMQLMATTGHEYEDTPGLGWVDGDVVKITPAEAHLKVPHMGWNDLVINHPHPVFDGISTGDHCYFVHSYHFR
VANPNERLAHVDYAGDVTAVIGRDTMLGMQFHPEKSQHVGLKMIGNFLTWKP
>Mature_212_residues
MLTAIIDYESGNLHSAEKAFQRMATEVGAGDVVVTSDADVVARADRLVLPGDGAFPACAAELRGHKGIYDAMVEAVEVKG
RPFLGICVGMQLMATTGHEYEDTPGLGWVDGDVVKITPAEAHLKVPHMGWNDLVINHPHPVFDGISTGDHCYFVHSYHFR
VANPNERLAHVDYAGDVTAVIGRDTMLGMQFHPEKSQHVGLKMIGNFLTWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=37.0192307692308, Blast_Score=122, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=217, Percent_Identity=30.4147465437788, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 23074; Mature: 23074

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAIIDYESGNLHSAEKAFQRMATEVGAGDVVVTSDADVVARADRLVLPGDGAFPACAA
CEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHEECCCEEEECCCCCCHHHHH
ELRGHKGIYDAMVEAVEVKGRPFLGICVGMQLMATTGHEYEDTPGLGWVDGDVVKITPAE
HHCCCCCHHHHHHHHHHHCCCCEEEHHHCEEEEECCCCCCCCCCCCCCCCCCEEEEECCH
AHLKVPHMGWNDLVINHPHPVFDGISTGDHCYFVHSYHFRVANPNERLAHVDYAGDVTAV
HEEECCCCCCCCEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEECCCCEEEE
IGRDTMLGMQFHPEKSQHVGLKMIGNFLTWKP
ECCCEEEEEEECCCCCCCCCHHHHCCHHCCCC
>Mature Secondary Structure
MLTAIIDYESGNLHSAEKAFQRMATEVGAGDVVVTSDADVVARADRLVLPGDGAFPACAA
CEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCHHEECCCEEEECCCCCCHHHHH
ELRGHKGIYDAMVEAVEVKGRPFLGICVGMQLMATTGHEYEDTPGLGWVDGDVVKITPAE
HHCCCCCHHHHHHHHHHHCCCCEEEHHHCEEEEECCCCCCCCCCCCCCCCCCEEEEECCH
AHLKVPHMGWNDLVINHPHPVFDGISTGDHCYFVHSYHFRVANPNERLAHVDYAGDVTAV
HEEECCCCCCCCEEEECCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEECCCCEEEE
IGRDTMLGMQFHPEKSQHVGLKMIGNFLTWKP
ECCCEEEEEEECCCCCCCCCHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA