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The map label for this gene is sdaA [H]

Identifier: 99081802

GI number: 99081802

Start: 2065987

End: 2067360

Strand: Direct

Name: sdaA [H]

Synonym: TM1040_1962

Alternate gene names: 99081802

Gene position: 2065987-2067360 (Clockwise)

Preceding gene: 99081801

Following gene: 99081804

Centisome position: 64.54

GC content: 63.83

Gene sequence:

>1374_bases
ATGTTCCTCTCCGTTTTTGACATGTTCAAAGTGGGCATCGGCCCGTCCTCCTCGCACACCATGGGCCCGATGGTGGCCGC
GGCACGCTTTCTCGACATGATGCGGGCCTCGCCCTTTGACTTTCACGGGCTGCGCGCATCGCTGCACGGCTCGCTGGCCT
TTACCGGCGTCGGCCATGCCACCGACCGCGCCACCATTCTCGGGCTTGCTGGCTTTTTGCCCGACACCTATGACGACGAC
AAAGCCGAGGCCGCGCTTGCGGCGATCACCGAGACCAAGACCATCGTGCTCGAGGATCTCGGCACGCTGCGCTTTGACCC
CAAGGCGGATATGATATTTGACTATGATCACGCGCTTCCGGGCCACGCCAACGGCATGGTTCTGATGGCGCTGGATGCGC
AGGGCGACGTGACCTTGCGGCAGGTGTTCTATTCCGTTGGCGGTGGCTTTGTGCTGACCGAGGAAGAGCTGGCACAGGGC
AAGGCCACGGATGAAGGCGACCCCGTCCCCTACCCGTTCAAATCCGCCGCCGAGATGCTCGAGATGGCAAAGACCTCCGG
GCTCTCGATCGCCCAGATGAAACGCGCCAATGAAGTCTCGCGCGGCTGCGAGCAGAGTTTTGCCAAGGGTACACAGCGCC
TGTGGCAGGTAATGAACGACTGTATCAACCGCGGGCTGGAGCGTGACGGCATCCTGCCCGGCGGTCTGAGCGTGCGCCGC
CGCGCCAAGGGTATCTATGATGCGCTGATGGCCGAGCGCGGCATGAACCTGACCGCGCCGCACACCATCAACGACTGGAT
GAGCGTCTATGCTATGGCCGTGAACGAGGAAAACGCCGCAGGCGGACAGGTCGTGACGGCCCCCACCAATGGCGCGGCGG
GCGTCCTGCCAGCGGTGCTGCGCTATTACCTTGATCACGTGCCGGGCGCGTCGGAAAAACACATCGAGGATTTCCTGCTG
ACCGCAGCCGCCATTGGCGGGCTGGTCAAATACAACGCCTCGATCTCTGGCGCCGAAGCGGGTTGTCAGGCCGAAGTCGG
CTCTGCCTCTGCCATGGCGGCGGCGGGGCTCTGTGCGGTGATGGGTGGCACGCCGGAACAGGTGGAGAACGCGGCCGAGA
TTGCGCTCGAACACCACCTCGGCATGACCTGCGACCCGGTCAAAGGGTTGGTTCAGGTGCCCTGCATCGAGCGCAACGGT
CTCGGCGCGATCAAGGCGGTTTCGGCAGCGTCCCTGGCGCTGCGCGGCGACGGGCAGCATTTTGTGCCGCTGGATGCCGT
CATCGAAACCATGCGCCAGACCGGGGCCGACATGCACGAGAAATACAAGGAAACCTCGCTTGGGGGCCTCGCCGTCAACG
TCCCCAACTGCTGA

Upstream 100 bases:

>100_bases
CCGCGCCAGCACCAAGGCGGCACAGACCGCCGAGGGGCTTGGCCCCACGCCCTTTACCACCCCGAAATTTGCCACCCCTC
CAGAAGGATCAGCCACCTAA

Downstream 100 bases:

>100_bases
GGCAGGCTCTCCCGCGCCATTCGCTGAGCGGGTGGCGCGGGCTTACCGCCGGATCATGGCTCTGCGGGGTTGCGCGCTGC
CTCTGCCGCGATTTCATCTG

Product: L-serine ammonia-lyase / 2-hydroxymethylglutarate dehydratase

Products: NA

Alternate protein names: SDH; L-serine deaminase; L-SD [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MFLSVFDMFKVGIGPSSSHTMGPMVAAARFLDMMRASPFDFHGLRASLHGSLAFTGVGHATDRATILGLAGFLPDTYDDD
KAEAALAAITETKTIVLEDLGTLRFDPKADMIFDYDHALPGHANGMVLMALDAQGDVTLRQVFYSVGGGFVLTEEELAQG
KATDEGDPVPYPFKSAAEMLEMAKTSGLSIAQMKRANEVSRGCEQSFAKGTQRLWQVMNDCINRGLERDGILPGGLSVRR
RAKGIYDALMAERGMNLTAPHTINDWMSVYAMAVNEENAAGGQVVTAPTNGAAGVLPAVLRYYLDHVPGASEKHIEDFLL
TAAAIGGLVKYNASISGAEAGCQAEVGSASAMAAAGLCAVMGGTPEQVENAAEIALEHHLGMTCDPVKGLVQVPCIERNG
LGAIKAVSAASLALRGDGQHFVPLDAVIETMRQTGADMHEKYKETSLGGLAVNVPNC

Sequences:

>Translated_457_residues
MFLSVFDMFKVGIGPSSSHTMGPMVAAARFLDMMRASPFDFHGLRASLHGSLAFTGVGHATDRATILGLAGFLPDTYDDD
KAEAALAAITETKTIVLEDLGTLRFDPKADMIFDYDHALPGHANGMVLMALDAQGDVTLRQVFYSVGGGFVLTEEELAQG
KATDEGDPVPYPFKSAAEMLEMAKTSGLSIAQMKRANEVSRGCEQSFAKGTQRLWQVMNDCINRGLERDGILPGGLSVRR
RAKGIYDALMAERGMNLTAPHTINDWMSVYAMAVNEENAAGGQVVTAPTNGAAGVLPAVLRYYLDHVPGASEKHIEDFLL
TAAAIGGLVKYNASISGAEAGCQAEVGSASAMAAAGLCAVMGGTPEQVENAAEIALEHHLGMTCDPVKGLVQVPCIERNG
LGAIKAVSAASLALRGDGQHFVPLDAVIETMRQTGADMHEKYKETSLGGLAVNVPNC
>Mature_457_residues
MFLSVFDMFKVGIGPSSSHTMGPMVAAARFLDMMRASPFDFHGLRASLHGSLAFTGVGHATDRATILGLAGFLPDTYDDD
KAEAALAAITETKTIVLEDLGTLRFDPKADMIFDYDHALPGHANGMVLMALDAQGDVTLRQVFYSVGGGFVLTEEELAQG
KATDEGDPVPYPFKSAAEMLEMAKTSGLSIAQMKRANEVSRGCEQSFAKGTQRLWQVMNDCINRGLERDGILPGGLSVRR
RAKGIYDALMAERGMNLTAPHTINDWMSVYAMAVNEENAAGGQVVTAPTNGAAGVLPAVLRYYLDHVPGASEKHIEDFLL
TAAAIGGLVKYNASISGAEAGCQAEVGSASAMAAAGLCAVMGGTPEQVENAAEIALEHHLGMTCDPVKGLVQVPCIERNG
LGAIKAVSAASLALRGDGQHFVPLDAVIETMRQTGADMHEKYKETSLGGLAVNVPNC

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI48994925, Length=455, Percent_Identity=47.9120879120879, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1789161, Length=459, Percent_Identity=46.8409586056645, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1788116, Length=456, Percent_Identity=46.4912280701754, Blast_Score=391, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 48211; Mature: 48211

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLSVFDMFKVGIGPSSSHTMGPMVAAARFLDMMRASPFDFHGLRASLHGSLAFTGVGHA
CEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEECCCC
TDRATILGLAGFLPDTYDDDKAEAALAAITETKTIVLEDLGTLRFDPKADMIFDYDHALP
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCEEECCCCCEEEECCCCCC
GHANGMVLMALDAQGDVTLRQVFYSVGGGFVLTEEELAQGKATDEGDPVPYPFKSAAEML
CCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHH
EMAKTSGLSIAQMKRANEVSRGCEQSFAKGTQRLWQVMNDCINRGLERDGILPGGLSVRR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RAKGIYDALMAERGMNLTAPHTINDWMSVYAMAVNEENAAGGQVVTAPTNGAAGVLPAVL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCCCCEEEECCCCCCHHHHHHHH
RYYLDHVPGASEKHIEDFLLTAAAIGGLVKYNASISGAEAGCQAEVGSASAMAAAGLCAV
HHHHHHCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MGGTPEQVENAAEIALEHHLGMTCDPVKGLVQVPCIERNGLGAIKAVSAASLALRGDGQH
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHHEEEECCCCC
FVPLDAVIETMRQTGADMHEKYKETSLGGLAVNVPNC
CCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCC
>Mature Secondary Structure
MFLSVFDMFKVGIGPSSSHTMGPMVAAARFLDMMRASPFDFHGLRASLHGSLAFTGVGHA
CEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEECCCC
TDRATILGLAGFLPDTYDDDKAEAALAAITETKTIVLEDLGTLRFDPKADMIFDYDHALP
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCEEECCCCCEEEECCCCCC
GHANGMVLMALDAQGDVTLRQVFYSVGGGFVLTEEELAQGKATDEGDPVPYPFKSAAEML
CCCCCEEEEEECCCCCHHHHHHHHHHCCCEEEEHHHHHCCCCCCCCCCCCCCHHHHHHHH
EMAKTSGLSIAQMKRANEVSRGCEQSFAKGTQRLWQVMNDCINRGLERDGILPGGLSVRR
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RAKGIYDALMAERGMNLTAPHTINDWMSVYAMAVNEENAAGGQVVTAPTNGAAGVLPAVL
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEECCCCCCCCEEEECCCCCCHHHHHHHH
RYYLDHVPGASEKHIEDFLLTAAAIGGLVKYNASISGAEAGCQAEVGSASAMAAAGLCAV
HHHHHHCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MGGTPEQVENAAEIALEHHLGMTCDPVKGLVQVPCIERNGLGAIKAVSAASLALRGDGQH
CCCCHHHHHHHHHHHHHHHCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHHEEEECCCCC
FVPLDAVIETMRQTGADMHEKYKETSLGGLAVNVPNC
CCCHHHHHHHHHHCCCHHHHHHHHHCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12000953 [H]