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The map label for this gene is yfcG [H]

Identifier: 99081772

GI number: 99081772

Start: 2030640

End: 2031323

Strand: Reverse

Name: yfcG [H]

Synonym: TM1040_1931

Alternate gene names: 99081772

Gene position: 2031323-2030640 (Counterclockwise)

Preceding gene: 99081773

Following gene: 99081767

Centisome position: 63.46

GC content: 57.75

Gene sequence:

>684_bases
TTGACGCGGCCCCTCGGGTCGCGTCACTCTGCGCTGCAGAAACGACTCGCGGAGACCCTCATGATCGACCTATACACCTG
GAACACCCCCAACGGGCGCAAGGTCTCCATCCTGCTCGAAGAGTTGAACGTACCTTATACCGCACATGCGATCGACATTG
CGACGGGCGCGCAAATGGCGCCCGCGTTTCTTGCGATCAGCCCGAATAACAAGATCCCGGCCATCCGCGATCAAGAGACC
GGTGTCACCTTGATGGAGTCAGGCGCGATCCTGATTTATCTTGCTGAAAAACATGGTCAATTCTTGCCGCCTGAACAGCG
GATCGAGATCTATGAATGGCTGATGTGGCAGATGGCTGGTTTTGGACCGATGCTGGGGCAGGCGCACCATTTCCTAAAGT
ACAACCGTGGCGCCTCCGACTATGCAGAGGAGCGCTATGGTGCCGAGGCGCGGCGTCTCTACGGCGTTCTGGATCGCCGT
CTTGAGGGGCGTGACTATATCGTCAACGACTATTCGATTGCCGATATGGCCTGCTGGCCATGGGTGTCGCGCTATGAATG
GCAGGACATCGATCTACGCGATTTCCCCAATGTGCGCAGATGGTACCAGGCCATTGCGGCCCGTCCGGCCGTGCAGCGCG
GATATCATTTGCCCGATCATGTCAATGAGATCCCGCAGGGTTGA

Upstream 100 bases:

>100_bases
CAGTCGCTTTCGGCTGTACGCGCCGAAGGACAGGGCAAGGCAAACGCCTGAGGCACGTTCTGCCTCTGGGTGCGCCAAAC
AATTCGTGACGGTGACTTGA

Downstream 100 bases:

>100_bases
GGCCCCGTTGGTCTGACGTTCTCTAGTTTGAGAGCTCTCCCATGACCTCCCGGGCCATGCGTTCGATGGCCGGGCGGGCC
TCTTCTGCGCTCATTCCGAC

Product: glutathione S-transferase-like

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTRPLGSRHSALQKRLAETLMIDLYTWNTPNGRKVSILLEELNVPYTAHAIDIATGAQMAPAFLAISPNNKIPAIRDQET
GVTLMESGAILIYLAEKHGQFLPPEQRIEIYEWLMWQMAGFGPMLGQAHHFLKYNRGASDYAEERYGAEARRLYGVLDRR
LEGRDYIVNDYSIADMACWPWVSRYEWQDIDLRDFPNVRRWYQAIAARPAVQRGYHLPDHVNEIPQG

Sequences:

>Translated_227_residues
MTRPLGSRHSALQKRLAETLMIDLYTWNTPNGRKVSILLEELNVPYTAHAIDIATGAQMAPAFLAISPNNKIPAIRDQET
GVTLMESGAILIYLAEKHGQFLPPEQRIEIYEWLMWQMAGFGPMLGQAHHFLKYNRGASDYAEERYGAEARRLYGVLDRR
LEGRDYIVNDYSIADMACWPWVSRYEWQDIDLRDFPNVRRWYQAIAARPAVQRGYHLPDHVNEIPQG
>Mature_226_residues
TRPLGSRHSALQKRLAETLMIDLYTWNTPNGRKVSILLEELNVPYTAHAIDIATGAQMAPAFLAISPNNKIPAIRDQETG
VTLMESGAILIYLAEKHGQFLPPEQRIEIYEWLMWQMAGFGPMLGQAHHFLKYNRGASDYAEERYGAEARRLYGVLDRRL
EGRDYIVNDYSIADMACWPWVSRYEWQDIDLRDFPNVRRWYQAIAARPAVQRGYHLPDHVNEIPQG

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=199, Percent_Identity=49.2462311557789, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI87082195, Length=212, Percent_Identity=40.5660377358491, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI87081789, Length=202, Percent_Identity=29.7029702970297, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324100, Length=225, Percent_Identity=32, Blast_Score=102, Evalue=6e-23,
Organism=Drosophila melanogaster, GI17933730, Length=190, Percent_Identity=29.4736842105263, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571670, Length=170, Percent_Identity=32.9411764705882, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17864596, Length=189, Percent_Identity=29.1005291005291, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17864592, Length=177, Percent_Identity=28.8135593220339, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI17864598, Length=178, Percent_Identity=30.3370786516854, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI17864594, Length=190, Percent_Identity=30.5263157894737, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45549270, Length=176, Percent_Identity=28.9772727272727, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI85725204, Length=190, Percent_Identity=28.4210526315789, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17737923, Length=190, Percent_Identity=28.4210526315789, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221378983, Length=179, Percent_Identity=27.3743016759777, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646259, Length=179, Percent_Identity=27.3743016759777, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646251, Length=192, Percent_Identity=27.6041666666667, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26160; Mature: 26029

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPLGSRHSALQKRLAETLMIDLYTWNTPNGRKVSILLEELNVPYTAHAIDIATGAQMA
CCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCC
PAFLAISPNNKIPAIRDQETGVTLMESGAILIYLAEKHGQFLPPEQRIEIYEWLMWQMAG
CEEEEECCCCCCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHC
FGPMLGQAHHFLKYNRGASDYAEERYGAEARRLYGVLDRRLEGRDYIVNDYSIADMACWP
CCHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCC
WVSRYEWQDIDLRDFPNVRRWYQAIAARPAVQRGYHLPDHVNEIPQG
CCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHCCCCCCHHHHHCCCC
>Mature Secondary Structure 
TRPLGSRHSALQKRLAETLMIDLYTWNTPNGRKVSILLEELNVPYTAHAIDIATGAQMA
CCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCC
PAFLAISPNNKIPAIRDQETGVTLMESGAILIYLAEKHGQFLPPEQRIEIYEWLMWQMAG
CEEEEECCCCCCCCCCCCCCCEEEEECCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHC
FGPMLGQAHHFLKYNRGASDYAEERYGAEARRLYGVLDRRLEGRDYIVNDYSIADMACWP
CCHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHCC
WVSRYEWQDIDLRDFPNVRRWYQAIAARPAVQRGYHLPDHVNEIPQG
CCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]