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The map label for this gene is thyA

Identifier: 99081570

GI number: 99081570

Start: 1825228

End: 1826061

Strand: Reverse

Name: thyA

Synonym: TM1040_1729

Alternate gene names: 99081570

Gene position: 1826061-1825228 (Counterclockwise)

Preceding gene: 99081572

Following gene: 99081569

Centisome position: 57.05

GC content: 55.76

Gene sequence:

>834_bases
ATGCAGCAGTATCATGACGCTTTGCAGCATATTCTTGATCACGGGGTCGAGACGACGGATCGTACCGGCACCGGCACTCT
GTCGTGTTTTGGAATGCAGCAACGTTATGATCTGGCCGAAGGGTTCCCTTTGGTGACGACCAAAAAGCTGCATCTGCGCT
CGATCATACATGAGCTGTTGTGGTTCCTGTCCGGGGATACAAACATAAGATATCTCAAAGAGAACGGTGTTTCGATCTGG
GACGAATGGGCGGATGAAAACGGCGATCTTGGTCCGGTCTATGGCTACCAGTGGCGCAAGTTTCCACGACTAGAGCTCGC
CGAGGGCACGCTTGGAGATGAGCCACTCTATCGGGCAGGCACGGTGGATCAGATCAGCGAGCTGCTGGATATGATCCGCA
AGAGCCCCGACAGCCGCCGTCTGATCGTCACCGCATGGAACCCCGGCGATGTGCCGGATATGGCGCTGCCGCCCTGTCAC
AGCCTGTGGCAACTGCGCATCCTGGGCGGCAAGATGCACCTGCAGCTCTACCAGCGCTCTGCGGATATGTTCCTCGGTGT
GCCGTTCAATATCGCTTCATATGCTTTGTTGTTGCACATGCTCGCCCATGTGACCGGCTATGAGGTGGGCAGTTTTGTGC
ATACGATGGGGGACGCGCATATCTATTCCAACCACATGGAACAGGTGAAACTGCAGCTCTCGCGCAGCCCCAAGTCCCTG
CCGCAGCTGCGCATTGCGCGCGATGTGTCTTCTATTTTTGATTTCAAATACGAGGATTTTGAAATCCTCGGCTATGATCC
CGACCCCGTCATCAAGGCCCCGGTCGCGGTCTAA

Upstream 100 bases:

>100_bases
AACCTAAGCCGCGCGTCCAGAATGTCGAGAGCGCGCGTAAACCGCTTGGAACTCTGCGATGCTCGGGCTATCACCGGAGG
GCAGACAGGAGGCTCGCACG

Downstream 100 bases:

>100_bases
GACCTCTCAGTTCAGGCAGGATTCCCCATGATCACGCTTATCGTCGCGCGGGCGCGAAACGGTGCCATTGGCAAAGACAA
CGACATCCCTTGGCACGCGC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MQQYHDALQHILDHGVETTDRTGTGTLSCFGMQQRYDLAEGFPLVTTKKLHLRSIIHELLWFLSGDTNIRYLKENGVSIW
DEWADENGDLGPVYGYQWRKFPRLELAEGTLGDEPLYRAGTVDQISELLDMIRKSPDSRRLIVTAWNPGDVPDMALPPCH
SLWQLRILGGKMHLQLYQRSADMFLGVPFNIASYALLLHMLAHVTGYEVGSFVHTMGDAHIYSNHMEQVKLQLSRSPKSL
PQLRIARDVSSIFDFKYEDFEILGYDPDPVIKAPVAV

Sequences:

>Translated_277_residues
MQQYHDALQHILDHGVETTDRTGTGTLSCFGMQQRYDLAEGFPLVTTKKLHLRSIIHELLWFLSGDTNIRYLKENGVSIW
DEWADENGDLGPVYGYQWRKFPRLELAEGTLGDEPLYRAGTVDQISELLDMIRKSPDSRRLIVTAWNPGDVPDMALPPCH
SLWQLRILGGKMHLQLYQRSADMFLGVPFNIASYALLLHMLAHVTGYEVGSFVHTMGDAHIYSNHMEQVKLQLSRSPKSL
PQLRIARDVSSIFDFKYEDFEILGYDPDPVIKAPVAV
>Mature_277_residues
MQQYHDALQHILDHGVETTDRTGTGTLSCFGMQQRYDLAEGFPLVTTKKLHLRSIIHELLWFLSGDTNIRYLKENGVSIW
DEWADENGDLGPVYGYQWRKFPRLELAEGTLGDEPLYRAGTVDQISELLDMIRKSPDSRRLIVTAWNPGDVPDMALPPCH
SLWQLRILGGKMHLQLYQRSADMFLGVPFNIASYALLLHMLAHVTGYEVGSFVHTMGDAHIYSNHMEQVKLQLSRSPKSL
PQLRIARDVSSIFDFKYEDFEILGYDPDPVIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=287, Percent_Identity=52.2648083623693, Blast_Score=303, Evalue=9e-83,
Organism=Escherichia coli, GI1789191, Length=277, Percent_Identity=59.927797833935, Blast_Score=349, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI71993377, Length=288, Percent_Identity=45.4861111111111, Blast_Score=265, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6324648, Length=232, Percent_Identity=44.8275862068966, Blast_Score=191, Evalue=8e-50,
Organism=Drosophila melanogaster, GI17137556, Length=288, Percent_Identity=50.3472222222222, Blast_Score=289, Evalue=1e-78,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_SILST (Q1GFV4)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613724.1
- ProteinModelPortal:   Q1GFV4
- SMR:   Q1GFV4
- STRING:   Q1GFV4
- GeneID:   4075793
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1729
- NMPDR:   fig|292414.1.peg.3202
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   Q1GFV4
- ProtClustDB:   PRK01827
- BioCyc:   SSP292414:TM1040_1729-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 31557; Mature: 31557

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 159-159

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYHDALQHILDHGVETTDRTGTGTLSCFGMQQRYDLAEGFPLVTTKKLHLRSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHCCCCCEECHHHHHHHHHHHHH
WFLSGDTNIRYLKENGVSIWDEWADENGDLGPVYGYQWRKFPRLELAEGTLGDEPLYRAG
HHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCC
TVDQISELLDMIRKSPDSRRLIVTAWNPGDVPDMALPPCHSLWQLRILGGKMHLQLYQRS
CHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHEEEEECCHHHHHHHHCC
ADMFLGVPFNIASYALLLHMLAHVTGYEVGSFVHTMGDAHIYSNHMEQVKLQLSRSPKSL
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
PQLRIARDVSSIFDFKYEDFEILGYDPDPVIKAPVAV
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MQQYHDALQHILDHGVETTDRTGTGTLSCFGMQQRYDLAEGFPLVTTKKLHLRSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHHHHCCCCCEECHHHHHHHHHHHHH
WFLSGDTNIRYLKENGVSIWDEWADENGDLGPVYGYQWRKFPRLELAEGTLGDEPLYRAG
HHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCC
TVDQISELLDMIRKSPDSRRLIVTAWNPGDVPDMALPPCHSLWQLRILGGKMHLQLYQRS
CHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCHHHHHEEEEECCHHHHHHHHCC
ADMFLGVPFNIASYALLLHMLAHVTGYEVGSFVHTMGDAHIYSNHMEQVKLQLSRSPKSL
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
PQLRIARDVSSIFDFKYEDFEILGYDPDPVIKAPVAV
HHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA