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The map label for this gene is dfrA [H]

Identifier: 99081569

GI number: 99081569

Start: 1824709

End: 1825200

Strand: Reverse

Name: dfrA [H]

Synonym: TM1040_1728

Alternate gene names: 99081569

Gene position: 1825200-1824709 (Counterclockwise)

Preceding gene: 99081570

Following gene: 99081567

Centisome position: 57.02

GC content: 57.93

Gene sequence:

>492_bases
ATGATCACGCTTATCGTCGCGCGGGCGCGAAACGGTGCCATTGGCAAAGACAACGACATCCCTTGGCACGCGCCCGAGGA
TCTGAAGGCGTTTCAGCGGGAAACGCTGGGAGGTGCGATCATCATGGGCCGCAACACCTGGGACAGTTTGCCATTCAAGC
CATTGAAAAACAGGTTCAATATCGTCGTGTCCTCAAACCCGGATGCGGCGGACACCGTGGTGGCCAGTCTTGATGAGGCA
ATCGATCTGGCCCGAAGCGAAGGCTACGCCCGCCTCTATGGTATCGGCGGGGCCGGGATCTACAAGGGCATGCTGGCACG
AGCTGATCGCTTGATGATTACCGAAGTGGACCTTGAAATAGATGATGCCGACGCGTTTTTCCCGGAGTTTCCTCCGGAGG
AGTGGCGTGTCAACGCGACGCTGCCTCTTCGTGCAGAGGACCCGGCCTGTGTCTTGACCGAATATCTGAGATTGCCTCGC
GCACAGGGATAA

Upstream 100 bases:

>100_bases
TTTCAAATACGAGGATTTTGAAATCCTCGGCTATGATCCCGACCCCGTCATCAAGGCCCCGGTCGCGGTCTAAGACCTCT
CAGTTCAGGCAGGATTCCCC

Downstream 100 bases:

>100_bases
TCTGCAGCAGAGGTGCATGGAAACGACCGCGCCCAAACCACACAACATGGCCTGGACGCAGTGGAGTTTTTTGGGAAAGG
GCCTTTAGAGTGGGCGAATG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MITLIVARARNGAIGKDNDIPWHAPEDLKAFQRETLGGAIIMGRNTWDSLPFKPLKNRFNIVVSSNPDAADTVVASLDEA
IDLARSEGYARLYGIGGAGIYKGMLARADRLMITEVDLEIDDADAFFPEFPPEEWRVNATLPLRAEDPACVLTEYLRLPR
AQG

Sequences:

>Translated_163_residues
MITLIVARARNGAIGKDNDIPWHAPEDLKAFQRETLGGAIIMGRNTWDSLPFKPLKNRFNIVVSSNPDAADTVVASLDEA
IDLARSEGYARLYGIGGAGIYKGMLARADRLMITEVDLEIDDADAFFPEFPPEEWRVNATLPLRAEDPACVLTEYLRLPR
AQG
>Mature_163_residues
MITLIVARARNGAIGKDNDIPWHAPEDLKAFQRETLGGAIIMGRNTWDSLPFKPLKNRFNIVVSSNPDAADTVVASLDEA
IDLARSEGYARLYGIGGAGIYKGMLARADRLMITEVDLEIDDADAFFPEFPPEEWRVNATLPLRAEDPACVLTEYLRLPR
AQG

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=160, Percent_Identity=31.25, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI28827793, Length=162, Percent_Identity=30.8641975308642, Blast_Score=67, Evalue=7e-12,
Organism=Escherichia coli, GI1786233, Length=137, Percent_Identity=41.6058394160584, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324810, Length=125, Percent_Identity=32.8, Blast_Score=63, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116008026, Length=173, Percent_Identity=30.0578034682081, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24647458, Length=173, Percent_Identity=30.0578034682081, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 17986; Mature: 17986

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLIVARARNGAIGKDNDIPWHAPEDLKAFQRETLGGAIIMGRNTWDSLPFKPLKNRFN
CEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEE
IVVSSNPDAADTVVASLDEAIDLARSEGYARLYGIGGAGIYKGMLARADRLMITEVDLEI
EEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCEEEEEEECEEE
DDADAFFPEFPPEEWRVNATLPLRAEDPACVLTEYLRLPRAQG
CCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MITLIVARARNGAIGKDNDIPWHAPEDLKAFQRETLGGAIIMGRNTWDSLPFKPLKNRFN
CEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCEEE
IVVSSNPDAADTVVASLDEAIDLARSEGYARLYGIGGAGIYKGMLARADRLMITEVDLEI
EEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHCCEEEEEEECEEE
DDADAFFPEFPPEEWRVNATLPLRAEDPACVLTEYLRLPRAQG
CCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]