Click here to switch to the map view.

The map label for this gene is nocT [H]

Identifier: 99081553

GI number: 99081553

Start: 1810083

End: 1810790

Strand: Direct

Name: nocT [H]

Synonym: TM1040_1712

Alternate gene names: 99081553

Gene position: 1810083-1810790 (Clockwise)

Preceding gene: 99081552

Following gene: 99081554

Centisome position: 56.55

GC content: 61.16

Gene sequence:

>708_bases
ATGAAATCTCTGCTTCTTGGCTCTGCCGCACTGGCACTGTCCGCAACCTTTGCCATGGCCGACACCATCCGCCTCGGCAC
CGAGGGCGCCTACCCTCCCTACAACTTCATCAACGACGCCGGTGAAGTCGACGGGTTCGAGCGCGAGCTGGGCGATGAGC
TGTGCAAGCGCGCCGAACTGACCTGCGAGTGGGTCACCAACGACTGGGATTCGATCATTCCGAACCTGCTGTCGGGCAAC
TACGACGCGATCATCGCGGGCATGTCGATCACCGACGAGCGCAAGGAAGTTGTTTCCTTCACCGAAGGCTACACCAAGCC
CTCGCCCTCCGCTTACGCCGCCCTGTCCGAAGGTGTTGACGTGAACGCAAGCGTTGTTGCCGCGCAGTCCGGCACCATCC
AGGCGGCACATGTGGCCGAAACCGGCGCCACCCTGGTGGAATTCCCCACCCCGGATGAGACCATCGCTGCCGTGAACTCC
GGCGAAGTCGATGTGGTGCTCTCCGACAAGGACTTCCTCGCACCGATCGTTGAAGAATCCGATCTGGTCTTCCTCGGCGA
CGTCTACCTCGGTGGTGGCATCGGCATGGCGTTCCGTCAGTCCGACGACGAGATGCGCGGCAAGTTCGATGCGGCGATTG
CCTCGATGAAAGAAGACGGTTCGCTCAACGAGCTGCTCGACAAATGGGAGATCGAGGGCAAGTTCTGA

Upstream 100 bases:

>100_bases
TGGAAATACCCGCAGTGAACGGCTGCGGGCCTTCCTGTCGTCCACGCGACAGGCCCAATAACCAAAAACCAGACCAACAC
CAATAAACGGGAGTTACCAC

Downstream 100 bases:

>100_bases
TCGCCCTGATCCCTGACTGAAACACGGGAGGGGGGCATCGGCCCCCTTTCCACTTTTGCCACCGCGCATGCGGTCGCGCC
CGTTCCCCATCAGATGACGA

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKSLLLGSAALALSATFAMADTIRLGTEGAYPPYNFINDAGEVDGFERELGDELCKRAELTCEWVTNDWDSIIPNLLSGN
YDAIIAGMSITDERKEVVSFTEGYTKPSPSAYAALSEGVDVNASVVAAQSGTIQAAHVAETGATLVEFPTPDETIAAVNS
GEVDVVLSDKDFLAPIVEESDLVFLGDVYLGGGIGMAFRQSDDEMRGKFDAAIASMKEDGSLNELLDKWEIEGKF

Sequences:

>Translated_235_residues
MKSLLLGSAALALSATFAMADTIRLGTEGAYPPYNFINDAGEVDGFERELGDELCKRAELTCEWVTNDWDSIIPNLLSGN
YDAIIAGMSITDERKEVVSFTEGYTKPSPSAYAALSEGVDVNASVVAAQSGTIQAAHVAETGATLVEFPTPDETIAAVNS
GEVDVVLSDKDFLAPIVEESDLVFLGDVYLGGGIGMAFRQSDDEMRGKFDAAIASMKEDGSLNELLDKWEIEGKF
>Mature_235_residues
MKSLLLGSAALALSATFAMADTIRLGTEGAYPPYNFINDAGEVDGFERELGDELCKRAELTCEWVTNDWDSIIPNLLSGN
YDAIIAGMSITDERKEVVSFTEGYTKPSPSAYAALSEGVDVNASVVAAQSGTIQAAHVAETGATLVEFPTPDETIAAVNS
GEVDVVLSDKDFLAPIVEESDLVFLGDVYLGGGIGMAFRQSDDEMRGKFDAAIASMKEDGSLNELLDKWEIEGKF

Specific function: Component of the nopaline active transport system probably consisting of four subunits:Q, M, P and T. This system is also capable of transporting octopine provided that catabolic functions are induced with nopaline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=246, Percent_Identity=29.2682926829268, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1787088, Length=245, Percent_Identity=29.7959183673469, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787085, Length=242, Percent_Identity=28.5123966942149, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1788648, Length=237, Percent_Identity=29.957805907173, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787031, Length=243, Percent_Identity=29.2181069958848, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788228, Length=218, Percent_Identity=26.605504587156, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 25068; Mature: 25068

Theoretical pI: Translated: 3.81; Mature: 3.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLLLGSAALALSATFAMADTIRLGTEGAYPPYNFINDAGEVDGFERELGDELCKRAEL
CCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCC
TCEWVTNDWDSIIPNLLSGNYDAIIAGMSITDERKEVVSFTEGYTKPSPSAYAALSEGVD
EEEEECCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VNASVVAAQSGTIQAAHVAETGATLVEFPTPDETIAAVNSGEVDVVLSDKDFLAPIVEES
CCEEEEEECCCCEEEEEECCCCCEEEECCCCCHHEEEECCCCEEEEECCCHHHHHHCCCC
DLVFLGDVYLGGGIGMAFRQSDDEMRGKFDAAIASMKEDGSLNELLDKWEIEGKF
CEEEEECEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSLLLGSAALALSATFAMADTIRLGTEGAYPPYNFINDAGEVDGFERELGDELCKRAEL
CCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHCC
TCEWVTNDWDSIIPNLLSGNYDAIIAGMSITDERKEVVSFTEGYTKPSPSAYAALSEGVD
EEEEECCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
VNASVVAAQSGTIQAAHVAETGATLVEFPTPDETIAAVNSGEVDVVLSDKDFLAPIVEES
CCEEEEEECCCCEEEEEECCCCCEEEECCCCCHHEEEECCCCEEEEECCCHHHHHHCCCC
DLVFLGDVYLGGGIGMAFRQSDDEMRGKFDAAIASMKEDGSLNELLDKWEIEGKF
CEEEEECEEECCCCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1732218; 11743193; 11743194 [H]