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The map label for this gene is metF [H]

Identifier: 99081541

GI number: 99081541

Start: 1796495

End: 1797364

Strand: Direct

Name: metF [H]

Synonym: TM1040_1700

Alternate gene names: 99081541

Gene position: 1796495-1797364 (Clockwise)

Preceding gene: 99081538

Following gene: 99081542

Centisome position: 56.12

GC content: 60.92

Gene sequence:

>870_bases
GTGACCACGCCCGAGATTTCCTTTGAATTCTTCCCGCCCAAGAACCTCGAAGCCTCTTTTCGGCTTTGGGACACGGTGCA
GTCACTGGCGCCGCTGGCGCCCCGTTTTGTCTCTGTGACCTATGGCGCGGGCGGCACCACCCGCGAGCTGACCCGCGATG
CGGTGACGACCCTGCACAAGAATTCCGGCCTCAAGGTGGCTGCACATTTGACCTGTGTTTCTGCGACACGACAGGAAACA
CTTGATATCGCAGACCAATATGCAGCAGCCGGGATAACCGACATCGTGGCCCTGCGCGGTGACCCGCCCAAGGAAGAAGG
TCGTTTCACGCCGACCGCGGACGGGTTCAACTCTTCGGTGGAGCTGATTGAGGCGCTCGCCGCAACCGGCAAGTTCAACC
TGCGCGTTGGCGCCTACCCCGAGAGCCATCCGGAAGCCGCCAGCCAAGCGGCCAATGTAGAATGGCTCAAGCGCAAGCTT
GATGCCGGTGCCGATGAGGCGCTCACGCAGTTCTTCTTTGAGGCCGAGAGCTTCTTTCGGTTCCGCGACGCCTGCGCCAA
GGCGGGCATCGACACTGACAAGATCACGCCCGGCATCCTGCCAATTGAAAACTGGAAGGGCGTGCGTCGTTTTGCAGAGG
CCTGCGGCACCACGATCCCGGCTTGGCTGGACGAGGCGTTCGAGAAAGCAGACCGCGACGATCGATGCGATCTCCTCGCC
ACAGCATTGTGCAGCGAGCTCTGCACGGAACTCCTGGATGGCGGCGTCAAGAAGCTGCATTTCTACACGCTGAACCGCCC
TGAACTTACCCGCGATGTGTGTCACGCGCTCGGTGTGAAATCCAAGGCACGCCTTCAGAACGTCGCGTAA

Upstream 100 bases:

>100_bases
TCATGTGAAAAGGGGTCAAACCTGAAGCGACACATGTGTCGCAAAACACGACATTCCGAGCCCGCCGTGAAACGCGCGCC
GCCTCCGAGGAGCACCGACA

Downstream 100 bases:

>100_bases
CGCTTGTTACGCCTGTGGCGCCCTCCTAACGTTTGTGAAATGCATTCAGGAGGACGCCATGGCCGTCGCGGAAACACCTT
CTGATCTGTTGCCGATCGAA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTTPEISFEFFPPKNLEASFRLWDTVQSLAPLAPRFVSVTYGAGGTTRELTRDAVTTLHKNSGLKVAAHLTCVSATRQET
LDIADQYAAAGITDIVALRGDPPKEEGRFTPTADGFNSSVELIEALAATGKFNLRVGAYPESHPEAASQAANVEWLKRKL
DAGADEALTQFFFEAESFFRFRDACAKAGIDTDKITPGILPIENWKGVRRFAEACGTTIPAWLDEAFEKADRDDRCDLLA
TALCSELCTELLDGGVKKLHFYTLNRPELTRDVCHALGVKSKARLQNVA

Sequences:

>Translated_289_residues
MTTPEISFEFFPPKNLEASFRLWDTVQSLAPLAPRFVSVTYGAGGTTRELTRDAVTTLHKNSGLKVAAHLTCVSATRQET
LDIADQYAAAGITDIVALRGDPPKEEGRFTPTADGFNSSVELIEALAATGKFNLRVGAYPESHPEAASQAANVEWLKRKL
DAGADEALTQFFFEAESFFRFRDACAKAGIDTDKITPGILPIENWKGVRRFAEACGTTIPAWLDEAFEKADRDDRCDLLA
TALCSELCTELLDGGVKKLHFYTLNRPELTRDVCHALGVKSKARLQNVA
>Mature_288_residues
TTPEISFEFFPPKNLEASFRLWDTVQSLAPLAPRFVSVTYGAGGTTRELTRDAVTTLHKNSGLKVAAHLTCVSATRQETL
DIADQYAAAGITDIVALRGDPPKEEGRFTPTADGFNSSVELIEALAATGKFNLRVGAYPESHPEAASQAANVEWLKRKLD
AGADEALTQFFFEAESFFRFRDACAKAGIDTDKITPGILPIENWKGVRRFAEACGTTIPAWLDEAFEKADRDDRCDLLAT
ALCSELCTELLDGGVKKLHFYTLNRPELTRDVCHALGVKSKARLQNVA

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=282, Percent_Identity=34.7517730496454, Blast_Score=155, Evalue=4e-38,
Organism=Escherichia coli, GI1790377, Length=276, Percent_Identity=44.2028985507246, Blast_Score=239, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI25147436, Length=283, Percent_Identity=32.8621908127208, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25147441, Length=283, Percent_Identity=32.8621908127208, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321313, Length=270, Percent_Identity=31.4814814814815, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6325234, Length=279, Percent_Identity=32.6164874551971, Blast_Score=137, Evalue=3e-33,
Organism=Drosophila melanogaster, GI24662658, Length=255, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31618; Mature: 31487

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPEISFEFFPPKNLEASFRLWDTVQSLAPLAPRFVSVTYGAGGTTRELTRDAVTTLHK
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHHEEEEECCCCCHHHHHHHHHHHHHC
NSGLKVAAHLTCVSATRQETLDIADQYAAAGITDIVALRGDPPKEEGRFTPTADGFNSSV
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCCHHCCCCCCCCCCCCHHH
ELIEALAATGKFNLRVGAYPESHPEAASQAANVEWLKRKLDAGADEALTQFFFEAESFFR
HHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FRDACAKAGIDTDKITPGILPIENWKGVRRFAEACGTTIPAWLDEAFEKADRDDRCDLLA
HHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHH
TALCSELCTELLDGGVKKLHFYTLNRPELTRDVCHALGVKSKARLQNVA
HHHHHHHHHHHHCCCHHEEEEEECCCCHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
TTPEISFEFFPPKNLEASFRLWDTVQSLAPLAPRFVSVTYGAGGTTRELTRDAVTTLHK
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHHEEEEECCCCCHHHHHHHHHHHHHC
NSGLKVAAHLTCVSATRQETLDIADQYAAAGITDIVALRGDPPKEEGRFTPTADGFNSSV
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCCCCHHCCCCCCCCCCCCHHH
ELIEALAATGKFNLRVGAYPESHPEAASQAANVEWLKRKLDAGADEALTQFFFEAESFFR
HHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
FRDACAKAGIDTDKITPGILPIENWKGVRRFAEACGTTIPAWLDEAFEKADRDDRCDLLA
HHHHHHHCCCCCCCCCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHH
TALCSELCTELLDGGVKKLHFYTLNRPELTRDVCHALGVKSKARLQNVA
HHHHHHHHHHHHCCCHHEEEEEECCCCHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA