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The map label for this gene is glyA

Identifier: 99081420

GI number: 99081420

Start: 1688939

End: 1690234

Strand: Direct

Name: glyA

Synonym: TM1040_1579

Alternate gene names: 99081420

Gene position: 1688939-1690234 (Clockwise)

Preceding gene: 99081418

Following gene: 99081421

Centisome position: 52.76

GC content: 62.27

Gene sequence:

>1296_bases
ATGACTGACGCGACCCGCGACCCCGGTTTCTTCACCCAAAGCCTCTCTGAGCGCGACCCAGAGCTTTTTGGCGCAATCAC
CGATGAACTCGGTCGTCAGCGCGACGAGATCGAGCTGATCGCCTCCGAAAACATCGTCTCTGCTGCCGTCATGGAAGCGC
AGGGCTCTGTGCTGACCAACAAGTATGCCGAAGGCTACCCGGGCCGTCGCTATTATGGCGGCTGCCAGTATGTCGACGTG
GCCGAAAACCTCGCCATCGACCGGGCCAAGCAGCTGTTCAACTGCGAATTTGCCAATGTGCAGCCCAACTCCGGCTCGCA
GGCGAACCAGGGCGTGTTCCAGGCGATTCTGAAGCCCGGCGACACCATCCTCGGCATGGATCTCGCGTCCGGCGGTCACC
TCACCCATGGTGCAGCGCCAAACCAGTCCGGCAAATGGTTCAACGCGGTGCACTACGGCGTGCGCGAATCCGACTGCCTG
ATCGACTATGATCAGGTTCAGGCACTTGCGACCGAGCACCAGCCCAAGCTGATCATCGCCGGCGGCTCCGCGATCCCGCG
CCAGATCGATTTTGCCAAGTTCCGTGAAATCGCTGACAGCGTCGGTGCCTATCTGCTGGTGGACATGGCGCATTTCGCCG
GTCTTGTTGCAGCAGGCGAGCATCCTTCGCCCTTCCCGCATGCTGATGTGGCCACCACCACCACCCACAAGACCCTGCGC
GGCCCGCGTGGCGGCATGATCCTGACCAACAACCCGGATCTGGCAAAGAAATTCAACTCTGCCATCTTCCCGGGCATTCA
GGGCGGCCCGCTGATGCACGTGATCGCAGGCAAAGCCGCCGCCTTTGGCGAGGCGCTGAAGCCCGAGTTCAAATCCTACC
AGAAACAAGTGCGTGCAAACGCGGTTGCGCTGGCGGATGAGCTGATCAAAGGCGGTCTGGACATCGTGACCGGCGGCACC
GACACCCATGTGATGCTGGTAGACCTGCGCCCCAAAGGCGTGACGGGCAACATCGTCGACAAAGCGCTCGGCCGTGCGCA
TATCACCACCAACAAGAACGGCATCCCGTTCGACCCGGAAAAGCCCACCGTGACCTCCGGTATCCGCCTCGGCACGCCTG
CAGGCACGACCCGTGGGTTTGGTGAGGAAGAGTTCCGCGAGATCGCCCGTCTGATCGTCGAGGTGGTCGACGGTCTCGCA
GCCAATGGCGAGGACGGCAACGCCGCGGTCGAAGAAGCCGTGCGTGGCAAAGTCGCGGCGCTCTGCGGCCGCTTCCCGCT
CTATCCAAACCTCTGA

Upstream 100 bases:

>100_bases
CGTTCACCTGATGCGTTTCGTCGCTTTACAGCCTTTCAGATCCGTGAAGTTTCCTATAGGAAATCTCGCAAATACCAAGC
CCCAACCAGACGGAGCCAAT

Downstream 100 bases:

>100_bases
TCTGGCGCCTCACCCGAGGTTGCTCTGCACCGCCGCGCTCTTTCGGGCGCGGCGGTTTTTTTTGCGCCGGTCCGCCTGGC
GTGGCTCGCCTCCCATACCC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTDATRDPGFFTQSLSERDPELFGAITDELGRQRDEIELIASENIVSAAVMEAQGSVLTNKYAEGYPGRRYYGGCQYVDV
AENLAIDRAKQLFNCEFANVQPNSGSQANQGVFQAILKPGDTILGMDLASGGHLTHGAAPNQSGKWFNAVHYGVRESDCL
IDYDQVQALATEHQPKLIIAGGSAIPRQIDFAKFREIADSVGAYLLVDMAHFAGLVAAGEHPSPFPHADVATTTTHKTLR
GPRGGMILTNNPDLAKKFNSAIFPGIQGGPLMHVIAGKAAAFGEALKPEFKSYQKQVRANAVALADELIKGGLDIVTGGT
DTHVMLVDLRPKGVTGNIVDKALGRAHITTNKNGIPFDPEKPTVTSGIRLGTPAGTTRGFGEEEFREIARLIVEVVDGLA
ANGEDGNAAVEEAVRGKVAALCGRFPLYPNL

Sequences:

>Translated_431_residues
MTDATRDPGFFTQSLSERDPELFGAITDELGRQRDEIELIASENIVSAAVMEAQGSVLTNKYAEGYPGRRYYGGCQYVDV
AENLAIDRAKQLFNCEFANVQPNSGSQANQGVFQAILKPGDTILGMDLASGGHLTHGAAPNQSGKWFNAVHYGVRESDCL
IDYDQVQALATEHQPKLIIAGGSAIPRQIDFAKFREIADSVGAYLLVDMAHFAGLVAAGEHPSPFPHADVATTTTHKTLR
GPRGGMILTNNPDLAKKFNSAIFPGIQGGPLMHVIAGKAAAFGEALKPEFKSYQKQVRANAVALADELIKGGLDIVTGGT
DTHVMLVDLRPKGVTGNIVDKALGRAHITTNKNGIPFDPEKPTVTSGIRLGTPAGTTRGFGEEEFREIARLIVEVVDGLA
ANGEDGNAAVEEAVRGKVAALCGRFPLYPNL
>Mature_430_residues
TDATRDPGFFTQSLSERDPELFGAITDELGRQRDEIELIASENIVSAAVMEAQGSVLTNKYAEGYPGRRYYGGCQYVDVA
ENLAIDRAKQLFNCEFANVQPNSGSQANQGVFQAILKPGDTILGMDLASGGHLTHGAAPNQSGKWFNAVHYGVRESDCLI
DYDQVQALATEHQPKLIIAGGSAIPRQIDFAKFREIADSVGAYLLVDMAHFAGLVAAGEHPSPFPHADVATTTTHKTLRG
PRGGMILTNNPDLAKKFNSAIFPGIQGGPLMHVIAGKAAAFGEALKPEFKSYQKQVRANAVALADELIKGGLDIVTGGTD
THVMLVDLRPKGVTGNIVDKALGRAHITTNKNGIPFDPEKPTVTSGIRLGTPAGTTRGFGEEEFREIARLIVEVVDGLAA
NGEDGNAAVEEAVRGKVAALCGRFPLYPNL

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI261862352, Length=408, Percent_Identity=46.8137254901961, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI261862350, Length=408, Percent_Identity=46.8137254901961, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI261862348, Length=408, Percent_Identity=46.8137254901961, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI19923315, Length=408, Percent_Identity=46.8137254901961, Blast_Score=344, Evalue=8e-95,
Organism=Homo sapiens, GI22547186, Length=459, Percent_Identity=44.4444444444444, Blast_Score=331, Evalue=7e-91,
Organism=Homo sapiens, GI261862346, Length=408, Percent_Identity=45.5882352941176, Blast_Score=328, Evalue=6e-90,
Organism=Homo sapiens, GI22547189, Length=445, Percent_Identity=42.4719101123596, Blast_Score=298, Evalue=5e-81,
Organism=Escherichia coli, GI1788902, Length=417, Percent_Identity=58.5131894484413, Blast_Score=470, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI25144732, Length=416, Percent_Identity=46.875, Blast_Score=345, Evalue=2e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=416, Percent_Identity=46.875, Blast_Score=345, Evalue=3e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=414, Percent_Identity=46.1352657004831, Blast_Score=353, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6323087, Length=411, Percent_Identity=46.2287104622871, Blast_Score=344, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24640005, Length=462, Percent_Identity=45.021645021645, Blast_Score=359, Evalue=2e-99,
Organism=Drosophila melanogaster, GI221329721, Length=462, Percent_Identity=45.021645021645, Blast_Score=358, Evalue=4e-99,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_SILST (Q1GGA4)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613574.1
- ProteinModelPortal:   Q1GGA4
- SMR:   Q1GGA4
- STRING:   Q1GGA4
- GeneID:   4078388
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1579
- NMPDR:   fig|292414.1.peg.3323
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   CEGLEMI
- PhylomeDB:   Q1GGA4
- ProtClustDB:   PRK00011
- BioCyc:   SSP292414:TM1040_1579-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45908; Mature: 45777

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDATRDPGFFTQSLSERDPELFGAITDELGRQRDEIELIASENIVSAAVMEAQGSVLTN
CCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCHHHH
KYAEGYPGRRYYGGCQYVDVAENLAIDRAKQLFNCEFANVQPNSGSQANQGVFQAILKPG
HHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHHHCCC
DTILGMDLASGGHLTHGAAPNQSGKWFNAVHYGVRESDCLIDYDQVQALATEHQPKLIIA
CEEEEEEECCCCCCCCCCCCCCCCCEEHHHHCCCCCCCCEECHHHHHHHHHCCCCEEEEE
GGSAIPRQIDFAKFREIADSVGAYLLVDMAHFAGLVAAGEHPSPFPHADVATTTTHKTLR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHC
GPRGGMILTNNPDLAKKFNSAIFPGIQGGPLMHVIAGKAAAFGEALKPEFKSYQKQVRAN
CCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHH
AVALADELIKGGLDIVTGGTDTHVMLVDLRPKGVTGNIVDKALGRAHITTNKNGIPFDPE
HHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCHHHHHHCCCEEEEECCCCCCCCCC
KPTVTSGIRLGTPAGTTRGFGEEEFREIARLIVEVVDGLAANGEDGNAAVEEAVRGKVAA
CCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LCGRFPLYPNL
HHCCCCCCCCC
>Mature Secondary Structure 
TDATRDPGFFTQSLSERDPELFGAITDELGRQRDEIELIASENIVSAAVMEAQGSVLTN
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCHHHH
KYAEGYPGRRYYGGCQYVDVAENLAIDRAKQLFNCEFANVQPNSGSQANQGVFQAILKPG
HHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHHHCCC
DTILGMDLASGGHLTHGAAPNQSGKWFNAVHYGVRESDCLIDYDQVQALATEHQPKLIIA
CEEEEEEECCCCCCCCCCCCCCCCCEEHHHHCCCCCCCCEECHHHHHHHHHCCCCEEEEE
GGSAIPRQIDFAKFREIADSVGAYLLVDMAHFAGLVAAGEHPSPFPHADVATTTTHKTLR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHC
GPRGGMILTNNPDLAKKFNSAIFPGIQGGPLMHVIAGKAAAFGEALKPEFKSYQKQVRAN
CCCCCEEEECCCHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHH
AVALADELIKGGLDIVTGGTDTHVMLVDLRPKGVTGNIVDKALGRAHITTNKNGIPFDPE
HHHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCHHHHHHCCCEEEEECCCCCCCCCC
KPTVTSGIRLGTPAGTTRGFGEEEFREIARLIVEVVDGLAANGEDGNAAVEEAVRGKVAA
CCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LCGRFPLYPNL
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA