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The map label for this gene is ppnK [H]

Identifier: 99081419

GI number: 99081419

Start: 1688007

End: 1688762

Strand: Reverse

Name: ppnK [H]

Synonym: TM1040_1578

Alternate gene names: 99081419

Gene position: 1688762-1688007 (Counterclockwise)

Preceding gene: 99081422

Following gene: 99081417

Centisome position: 52.76

GC content: 61.64

Gene sequence:

>756_bases
ATGAGAATCGCATTTCTGGCCAGCGAGGCCCCTTTGGCGCAGACGGCTCTTTCTGTTCTGAGCAAACGCTACGGATCTGT
CGCCGTCGAGGCCGCAGATGTTATCGTGGCGCTGGGGGGCGATGGGTTCATGTTGCACACGCTGCGTGACACCGTTGATC
TCAATATTCCGATCTACGGGATGAACCGCGGCACGGTTGGTTTCTTGATGAACAGCTTTGGCGAGGACGCGCTTCTGGAA
CGGCTGGAGGCCGCCAATGAAGAGATCATCCACCCCTTGCGGATGCGGGCGCAGGACCGGGCCGGGGTCATGCATGAGGC
GATGGCGATCAACGAAGTGTCCTTGCTGCGGCAGGGTCCGCAGGCGGCCAAGCTCAAGATCTCGGTTGATGGGCGGCAGC
GGATGGCGGAGCTGGTTTGCGATGGCGCGCTGGTGTCGACGCCTGCGGGCTCCACCGCCTATAATTTTTCGGCGCACGGC
CCGATCCTGCCCATCGGGGCGGATGTGCTGGCGCTGACGGCGATTGCGGCCTTTCGCCCAAGGCGCTGGCGCGGCGCGTT
GCTGCCCAGTGGGGCCACCGTGCGTTTTGACGTGATCGATGCGGCCAAGCGACCCGTGATGGCGGAAGCGGATTCGATTT
CCTTCAAGGAGATCGACTGGGTTGAAATCCAGAGTGAACGCCGGATCAGCCACAAGATCCTGTTTGACCCCGGTCATGGG
CTTGAGGAACGGCTGATCTCGGAACAGTTCAACTGA

Upstream 100 bases:

>100_bases
GCGACGAAACGCATCAGGTGAACGGCAACTCTGGTCAACGGGGAAAACCTGTGCTTGTTTCGGAACCGTTTGGACCCTAC
CGGAAACAGGATCGCGCCTT

Downstream 100 bases:

>100_bases
GGCCGCGCTGGCGCGCGACAGGTTGAGCCTTGGACCCATGGGTCCAAGGCCGTGCCTCCGGCGGGGATATTTCTGGTTAG
AAGAAGGCATCGGCGCGCAC

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRIAFLASEAPLAQTALSVLSKRYGSVAVEAADVIVALGGDGFMLHTLRDTVDLNIPIYGMNRGTVGFLMNSFGEDALLE
RLEAANEEIIHPLRMRAQDRAGVMHEAMAINEVSLLRQGPQAAKLKISVDGRQRMAELVCDGALVSTPAGSTAYNFSAHG
PILPIGADVLALTAIAAFRPRRWRGALLPSGATVRFDVIDAAKRPVMAEADSISFKEIDWVEIQSERRISHKILFDPGHG
LEERLISEQFN

Sequences:

>Translated_251_residues
MRIAFLASEAPLAQTALSVLSKRYGSVAVEAADVIVALGGDGFMLHTLRDTVDLNIPIYGMNRGTVGFLMNSFGEDALLE
RLEAANEEIIHPLRMRAQDRAGVMHEAMAINEVSLLRQGPQAAKLKISVDGRQRMAELVCDGALVSTPAGSTAYNFSAHG
PILPIGADVLALTAIAAFRPRRWRGALLPSGATVRFDVIDAAKRPVMAEADSISFKEIDWVEIQSERRISHKILFDPGHG
LEERLISEQFN
>Mature_251_residues
MRIAFLASEAPLAQTALSVLSKRYGSVAVEAADVIVALGGDGFMLHTLRDTVDLNIPIYGMNRGTVGFLMNSFGEDALLE
RLEAANEEIIHPLRMRAQDRAGVMHEAMAINEVSLLRQGPQAAKLKISVDGRQRMAELVCDGALVSTPAGSTAYNFSAHG
PILPIGADVLALTAIAAFRPRRWRGALLPSGATVRFDVIDAAKRPVMAEADSISFKEIDWVEIQSERRISHKILFDPGHG
LEERLISEQFN

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus [H]

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788968, Length=175, Percent_Identity=32.5714285714286, Blast_Score=64, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6325068, Length=177, Percent_Identity=27.683615819209, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504 [H]

Pfam domain/function: PF01513 NAD_kinase [H]

EC number: =2.7.1.23 [H]

Molecular weight: Translated: 27289; Mature: 27289

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAFLASEAPLAQTALSVLSKRYGSVAVEAADVIVALGGDGFMLHTLRDTVDLNIPIYG
CEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCEEEEEECCCEEECEEEEE
MNRGTVGFLMNSFGEDALLERLEAANEEIIHPLRMRAQDRAGVMHEAMAINEVSLLRQGP
CCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCC
QAAKLKISVDGRQRMAELVCDGALVSTPAGSTAYNFSAHGPILPIGADVLALTAIAAFRP
CCEEEEEEECHHHHHHHHHHCCCEEECCCCCCEEECCCCCCEEECCHHHHHHHHHHHHCC
RRWRGALLPSGATVRFDVIDAAKRPVMAEADSISFKEIDWVEIQSERRISHKILFDPGHG
CCCCCCCCCCCCEEEEEEEHHHCCCCEECCCCCCCCCCEEEEECCCCCCCEEEEECCCCC
LEERLISEQFN
HHHHHHHHCCC
>Mature Secondary Structure
MRIAFLASEAPLAQTALSVLSKRYGSVAVEAADVIVALGGDGFMLHTLRDTVDLNIPIYG
CEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCEEEEEECCCEEECEEEEE
MNRGTVGFLMNSFGEDALLERLEAANEEIIHPLRMRAQDRAGVMHEAMAINEVSLLRQGP
CCCCHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHCCC
QAAKLKISVDGRQRMAELVCDGALVSTPAGSTAYNFSAHGPILPIGADVLALTAIAAFRP
CCEEEEEEECHHHHHHHHHHCCCEEECCCCCCEEECCCCCCEEECCHHHHHHHHHHHHCC
RRWRGALLPSGATVRFDVIDAAKRPVMAEADSISFKEIDWVEIQSERRISHKILFDPGHG
CCCCCCCCCCCCEEEEEEEHHHCCCCEECCCCCCCCCCEEEEECCCCCCCEEEEECCCCC
LEERLISEQFN
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA