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The map label for this gene is phaE [H]

Identifier: 99081382

GI number: 99081382

Start: 1649139

End: 1649636

Strand: Direct

Name: phaE [H]

Synonym: TM1040_1541

Alternate gene names: 99081382

Gene position: 1649139-1649636 (Clockwise)

Preceding gene: 99081381

Following gene: 99081383

Centisome position: 51.52

GC content: 59.84

Gene sequence:

>498_bases
ATGAAACTCCTGAACCGACTGCTTCCGCACCCTCTTTTGACGCTGATGCTGACAGTTATCTGGCAGCTTCTGGTCAACAA
GGTCACGCCGAACTCGCTGCTGTTTGGGTTCTTCCTCGGGTTGCTGATCCCCTTTGTGACCCAGCCCTATTGGCCCAACC
GCCCCAAACTCAAGAGCCCGCTGCGGCTTGCGACCTATGTGCTTTTGGTGTTCTGGGACATCGTCATGGCCAACATCACG
GTGGCGCGCATCATCCTGTTCATGCCCAACAGCAAGCGTCAGCCCACCTGGGTGTCGATCCCGCTTGAGATCCGCGCGCC
CGAGGCCATCGCCATGCTGGGGGGCACCATCACCATGACCCCGGGCACGCTGACGGCGGATATTTCGTCGGACGGGCGCT
ATCTTCTGGTGCATTGCCTGCACGCGCCCAACCCGGATGAGGTGCGCGACGAGATCAAGACCCGCTACGAACGCCGCCTG
AAGGAGATTTTCGAATGA

Upstream 100 bases:

>100_bases
TTGCAAACCGCGACCGCCCGGTAGTTGAGCCCGACGCAGATGGCACAGACAAGGCCAAGGACGATGGCGCCGCTGCGCCC
AAATCGGAGGAGAGCAACTG

Downstream 100 bases:

>100_bases
TCGAATATGCCTATATCTTCGCCTTCGGCTGTTTCGCCCTGGCGATGCTGATGAACCTCTGGAAAGTCGTGACAGGCACA
GAGGTGACCGACCGGATCCT

Product: putative monovalent cation/H+ antiporter subunit E

Products: NA

Alternate protein names: pH adaptation potassium efflux system protein E; Pha system subunit E [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MKLLNRLLPHPLLTLMLTVIWQLLVNKVTPNSLLFGFFLGLLIPFVTQPYWPNRPKLKSPLRLATYVLLVFWDIVMANIT
VARIILFMPNSKRQPTWVSIPLEIRAPEAIAMLGGTITMTPGTLTADISSDGRYLLVHCLHAPNPDEVRDEIKTRYERRL
KEIFE

Sequences:

>Translated_165_residues
MKLLNRLLPHPLLTLMLTVIWQLLVNKVTPNSLLFGFFLGLLIPFVTQPYWPNRPKLKSPLRLATYVLLVFWDIVMANIT
VARIILFMPNSKRQPTWVSIPLEIRAPEAIAMLGGTITMTPGTLTADISSDGRYLLVHCLHAPNPDEVRDEIKTRYERRL
KEIFE
>Mature_165_residues
MKLLNRLLPHPLLTLMLTVIWQLLVNKVTPNSLLFGFFLGLLIPFVTQPYWPNRPKLKSPLRLATYVLLVFWDIVMANIT
VARIILFMPNSKRQPTWVSIPLEIRAPEAIAMLGGTITMTPGTLTADISSDGRYLLVHCLHAPNPDEVRDEIKTRYERRL
KEIFE

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG1863

COG function: function code P; Multisubunit Na+/H+ antiporter, MnhE subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004847
- InterPro:   IPR002758 [H]

Pfam domain/function: PF01899 MNHE [H]

EC number: NA

Molecular weight: Translated: 18960; Mature: 18960

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLNRLLPHPLLTLMLTVIWQLLVNKVTPNSLLFGFFLGLLIPFVTQPYWPNRPKLKSP
CCHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
LRLATYVLLVFWDIVMANITVARIILFMPNSKRQPTWVSIPLEIRAPEAIAMLGGTITMT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCHHHHHHCCEEEEC
PGTLTADISSDGRYLLVHCLHAPNPDEVRDEIKTRYERRLKEIFE
CCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLLNRLLPHPLLTLMLTVIWQLLVNKVTPNSLLFGFFLGLLIPFVTQPYWPNRPKLKSP
CCHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
LRLATYVLLVFWDIVMANITVARIILFMPNSKRQPTWVSIPLEIRAPEAIAMLGGTITMT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCHHHHHHCCEEEEC
PGTLTADISSDGRYLLVHCLHAPNPDEVRDEIKTRYERRLKEIFE
CCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]