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The map label for this gene is mrpD [H]

Identifier: 99081381

GI number: 99081381

Start: 1647517

End: 1649139

Strand: Direct

Name: mrpD [H]

Synonym: TM1040_1540

Alternate gene names: 99081381

Gene position: 1647517-1649139 (Clockwise)

Preceding gene: 99081380

Following gene: 99081382

Centisome position: 51.47

GC content: 61.31

Gene sequence:

>1623_bases
ATGAACCATCTTCTGATTGCGCCCATCGTGCTGCCCGCATTGGTGGCGCCATTCATCATTATGACCTTGCGCTATCACAT
TGATTTGCAGCGCATATTCTCTTTGACAACGAATGTGCTACTGGTTGCCATCGCGATGGCGCTGGCGGTACAGGCCGCAG
ATGGCACCGTGCAGGTCTACGAGCTCGGCGACTGGCAGGCGCCCTTTGGCATCGTGCTGGTGCTTGATCGGCTCTCGGCC
ATGATGGTGCTGCTGACCACCGTTCTGGCCCTTGCCGTAAACCTCTATGCAATCGGCAGCAAATGGGACGAGCGCGGCGC
GAACTTTCATGCGCTCTATCTCTTTCAGCTCATGGGCGTCATCGGCGCGTTCCTGACGGGCGATGCGTTCAACCTCTTTG
TGTTCTTCGAAGTGCTCTTGATCGCGTCCTACGGGCTAATGATCCACGGCGGTGGTGCCCGGCGGTTTCAGGCAGGCGTG
CAATATGTGGTGTTCAACCTTCTGGGTTCGACGCTGTTTCTGTTTGCGCTTGGCACCATCTACGGGGTGACCGGCACGCT
CAACCTCGCGGATCTTGCGCAGCGCGTCGCCGAACTTCCGGCTGAAGACAGCGCCCTTATTCGCGTGGGCGCGGTGATGC
TGCTTCTGGTCTTTGCGATCAAAGCCTCGCTGTTGCCGCTGCATTTCTGGCTGCCAGCCTCCTATGCCAATGCACCCGCG
CCGGTGGCCGCGCTCTTTGCGATCATGACCAAAGTTGGCGCCTATGCGATCCTGCGCGTCTATACGCTGGTGTTCGGACC
CGACGTCGCGGTGGTCGATGGGCTGGTGGAAAGCTGGTTGTTGCCTGCCGCGCTTTTGACCATTGCCATCGGGGCGATCG
GAGTTCTGGGTTCCAAGGAAATCGGCAAGCTGATGTCCTTTGGCGCGATCACCTCTGTGGGCACGCTGCTCACGGCGATT
GCGCTCTTTACGCCCGAGGCCACCTCGGCCGCGCTCTACTACACGGTGCATTCGACGCTGGCAGCCGCAGCGCTGTTCCT
CGTGGCCGACATGGTGCGCGAACGCCAAGGCGGGGACATAGCCCCCAAGCCAGTCATGGCGCAAAGCGGGCTCATCTCGG
TGCTCTTTTTCGTGGCGGCCATCGCCACGGCGGGCATGCCGCCTCTGTCCGGTTTCCTTGGCAAACTGCTGATCCTTGAC
GCGGCGCGCGACAATACGCTTGCAGTCTGGGTCTGGGGCGTGATCCTCGTGAGCTCTCTTGTGACCATCATGGGATTTGC
CCGCGCAGGCACTCTGATCTTCTGGAAAAGCCATGGTCTGCCCTCCGAGCAGCCCGCGGAGGAAGACGCAAGCGAGAGCG
CCACAACGACGACAGAGCGCACATCCGCCCTGCCCTTCGTGGCTTGTTTCTCGCTTCTGGCAGGGCTGGTCGCGCTGACC
CTCTTTGCCGGACCGGTGACCCGCTATACCACGGCCACCGCTGCGGATCTCTTTGCGCCCGAGGCCTATATCGAGACGGT
CCTTGCAAACCGCGACCGCCCGGTAGTTGAGCCCGACGCAGATGGCACAGACAAGGCCAAGGACGATGGCGCCGCTGCGC
CCAAATCGGAGGAGAGCAACTGA

Upstream 100 bases:

>100_bases
TGACCGCGGTTGTGGTGATGGTGGCGCTCGCCGCCTATCTCAGCGCCCGCGACGACAATATCAACATGGACGACACTGCG
CCTGCGCAGGGAGAGGACGC

Downstream 100 bases:

>100_bases
TGAAACTCCTGAACCGACTGCTTCCGCACCCTCTTTTGACGCTGATGCTGACAGTTATCTGGCAGCTTCTGGTCAACAAG
GTCACGCCGAACTCGCTGCT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 540; Mature: 540

Protein sequence:

>540_residues
MNHLLIAPIVLPALVAPFIIMTLRYHIDLQRIFSLTTNVLLVAIAMALAVQAADGTVQVYELGDWQAPFGIVLVLDRLSA
MMVLLTTVLALAVNLYAIGSKWDERGANFHALYLFQLMGVIGAFLTGDAFNLFVFFEVLLIASYGLMIHGGGARRFQAGV
QYVVFNLLGSTLFLFALGTIYGVTGTLNLADLAQRVAELPAEDSALIRVGAVMLLLVFAIKASLLPLHFWLPASYANAPA
PVAALFAIMTKVGAYAILRVYTLVFGPDVAVVDGLVESWLLPAALLTIAIGAIGVLGSKEIGKLMSFGAITSVGTLLTAI
ALFTPEATSAALYYTVHSTLAAAALFLVADMVRERQGGDIAPKPVMAQSGLISVLFFVAAIATAGMPPLSGFLGKLLILD
AARDNTLAVWVWGVILVSSLVTIMGFARAGTLIFWKSHGLPSEQPAEEDASESATTTTERTSALPFVACFSLLAGLVALT
LFAGPVTRYTTATAADLFAPEAYIETVLANRDRPVVEPDADGTDKAKDDGAAAPKSEESN

Sequences:

>Translated_540_residues
MNHLLIAPIVLPALVAPFIIMTLRYHIDLQRIFSLTTNVLLVAIAMALAVQAADGTVQVYELGDWQAPFGIVLVLDRLSA
MMVLLTTVLALAVNLYAIGSKWDERGANFHALYLFQLMGVIGAFLTGDAFNLFVFFEVLLIASYGLMIHGGGARRFQAGV
QYVVFNLLGSTLFLFALGTIYGVTGTLNLADLAQRVAELPAEDSALIRVGAVMLLLVFAIKASLLPLHFWLPASYANAPA
PVAALFAIMTKVGAYAILRVYTLVFGPDVAVVDGLVESWLLPAALLTIAIGAIGVLGSKEIGKLMSFGAITSVGTLLTAI
ALFTPEATSAALYYTVHSTLAAAALFLVADMVRERQGGDIAPKPVMAQSGLISVLFFVAAIATAGMPPLSGFLGKLLILD
AARDNTLAVWVWGVILVSSLVTIMGFARAGTLIFWKSHGLPSEQPAEEDASESATTTTERTSALPFVACFSLLAGLVALT
LFAGPVTRYTTATAADLFAPEAYIETVLANRDRPVVEPDADGTDKAKDDGAAAPKSEESN
>Mature_540_residues
MNHLLIAPIVLPALVAPFIIMTLRYHIDLQRIFSLTTNVLLVAIAMALAVQAADGTVQVYELGDWQAPFGIVLVLDRLSA
MMVLLTTVLALAVNLYAIGSKWDERGANFHALYLFQLMGVIGAFLTGDAFNLFVFFEVLLIASYGLMIHGGGARRFQAGV
QYVVFNLLGSTLFLFALGTIYGVTGTLNLADLAQRVAELPAEDSALIRVGAVMLLLVFAIKASLLPLHFWLPASYANAPA
PVAALFAIMTKVGAYAILRVYTLVFGPDVAVVDGLVESWLLPAALLTIAIGAIGVLGSKEIGKLMSFGAITSVGTLLTAI
ALFTPEATSAALYYTVHSTLAAAALFLVADMVRERQGGDIAPKPVMAQSGLISVLFFVAAIATAGMPPLSGFLGKLLILD
AARDNTLAVWVWGVILVSSLVTIMGFARAGTLIFWKSHGLPSEQPAEEDASESATTTTERTSALPFVACFSLLAGLVALT
LFAGPVTRYTTATAADLFAPEAYIETVLANRDRPVVEPDADGTDKAKDDGAAAPKSEESN

Specific function: Mrp complex is a Na(+)/H(+) antiporter involved in Na(+) excretion and Na(+) resistance [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=291, Percent_Identity=26.1168384879725, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1788613, Length=430, Percent_Identity=28.1395348837209, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1788829, Length=445, Percent_Identity=26.0674157303371, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788831, Length=385, Percent_Identity=23.6363636363636, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788827, Length=355, Percent_Identity=27.3239436619718, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI145693160, Length=362, Percent_Identity=23.4806629834254, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 57071; Mature: 57071

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHLLIAPIVLPALVAPFIIMTLRYHIDLQRIFSLTTNVLLVAIAMALAVQAADGTVQVY
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ELGDWQAPFGIVLVLDRLSAMMVLLTTVLALAVNLYAIGSKWDERGANFHALYLFQLMGV
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHH
IGAFLTGDAFNLFVFFEVLLIASYGLMIHGGGARRFQAGVQYVVFNLLGSTLFLFALGTI
HHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGVTGTLNLADLAQRVAELPAEDSALIRVGAVMLLLVFAIKASLLPLHFWLPASYANAPA
HHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCH
PVAALFAIMTKVGAYAILRVYTLVFGPDVAVVDGLVESWLLPAALLTIAIGAIGVLGSKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IGKLMSFGAITSVGTLLTAIALFTPEATSAALYYTVHSTLAAAALFLVADMVRERQGGDI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCC
APKPVMAQSGLISVLFFVAAIATAGMPPLSGFLGKLLILDAARDNTLAVWVWGVILVSSL
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCEEHHHHHHHHHHHHH
VTIMGFARAGTLIFWKSHGLPSEQPAEEDASESATTTTERTSALPFVACFSLLAGLVALT
HHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
LFAGPVTRYTTATAADLFAPEAYIETVLANRDRPVVEPDADGTDKAKDDGAAAPKSEESN
HHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNHLLIAPIVLPALVAPFIIMTLRYHIDLQRIFSLTTNVLLVAIAMALAVQAADGTVQVY
CCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
ELGDWQAPFGIVLVLDRLSAMMVLLTTVLALAVNLYAIGSKWDERGANFHALYLFQLMGV
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHH
IGAFLTGDAFNLFVFFEVLLIASYGLMIHGGGARRFQAGVQYVVFNLLGSTLFLFALGTI
HHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGVTGTLNLADLAQRVAELPAEDSALIRVGAVMLLLVFAIKASLLPLHFWLPASYANAPA
HHHCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCH
PVAALFAIMTKVGAYAILRVYTLVFGPDVAVVDGLVESWLLPAALLTIAIGAIGVLGSKE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
IGKLMSFGAITSVGTLLTAIALFTPEATSAALYYTVHSTLAAAALFLVADMVRERQGGDI
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCC
APKPVMAQSGLISVLFFVAAIATAGMPPLSGFLGKLLILDAARDNTLAVWVWGVILVSSL
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCEEHHHHHHHHHHHHH
VTIMGFARAGTLIFWKSHGLPSEQPAEEDASESATTTTERTSALPFVACFSLLAGLVALT
HHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHH
LFAGPVTRYTTATAADLFAPEAYIETVLANRDRPVVEPDADGTDKAKDDGAAAPKSEESN
HHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]