Click here to switch to the map view.

The map label for this gene is phrB [H]

Identifier: 99081282

GI number: 99081282

Start: 1538768

End: 1540192

Strand: Reverse

Name: phrB [H]

Synonym: TM1040_1441

Alternate gene names: 99081282

Gene position: 1540192-1538768 (Counterclockwise)

Preceding gene: 99081283

Following gene: 99081281

Centisome position: 48.12

GC content: 61.68

Gene sequence:

>1425_bases
ATGGCTGACACCCGACCGGTGATCTGGTGGATTCGGCGCGACCTGCGCCTGCGCGACAATCCAGCCCTCAGGGCCGCGCA
GGAGGCGGGCGGACCGATTATCCCGCTCTATATCCATGACGCGCAAGAAGAGGCCCTCGGGGCCGCGCCAAAGTTCCGCC
TTGGCCTCGGGCTTGAACGGTTTGCCAAAACGCTCGAGGAGAAAGGCAGCCGTCTCATCGTCCGCCGTGGCGACGCACTC
GAGGTGCTGCGCGAGGTCATCGCGGAGAGCGGCGCCGGACATGTGATCTGGTCGCGGCTCTACGATCCGGCGGCCACAAA
ACGTGACGCAAAGATCAAGGAAGCACTCAAAGCGTCTGATATAAAGGCAAAATCCACGGGTGGGCGGCTGCTATTCGAAC
CTTGGACCGTCGACACCAAGGACGGCGGCATGTACCGGGTATATACGCCGTTCTGGAAGGCAGTCCGTACGCGCGATGTC
GCGGAGCTGACTGCCGCCCCCTCCCGGTTGGCCGCGCCCGAGAGTTGGCCGTCAGGTGAAGCGCTCGCGGATCTTGGCAT
GGATGCCGCAATGCGTCGGGGTGGCGCGATTGTTGCGCAACATTGCCGTGTGGGAGAGGACGCAGCCCTCGCGCAACTGG
ATGATTTCCTGACGGAACGGGTTTCGAACTACAAAGCCCATCGGGATTTTCCCGCCCGTGCTGCCACCTCGGAGCTTTCG
GAAAATCTCGCCTGGGGGGAAATCAGCCCACATCGGATGTGGCATCTGGGCGCGCAGGCGATGCAGGACGGCCAGCCCGG
CGCGGAGCATTTCCTCAAAGAGGTTGTCTGGCGCGAGTTTGCCTATCACCTGATGCATCGCTCGCCACGCATCCTGACGC
GAAACTGGCGAGAGGAATGGGACGGTTTCCCCTGGCAGGACGACCCTGGCGACGCTTTGCAGCGCTGGCAACAGGGACAG
ACCGGCTATGATTTTGTCGATGCCGCGATGCGAGAGCTCTACGTCACCGGCAAGATGCACAACCGCGCTCGGATGATCGT
GGCAAGCTTTCTTACCAAGCATCTGATGATCCATTGGAAATACGGATTGCAGTGGTTCGAGGACTGTCTTGTCGACTGGG
ATCCCGCTTCGAACGCGATGGGCTGGCAGTGGGTGGCGGGTTCCGGTCCTGATGCGGCGCCCTTTTTTCGCATTTTCAAC
CCGGATGGTCAGCTCGAGAAGTTCGACCCCAAGGGAGACTATGCGCGTCGGTGGCTGCCCGAAGGCCAGGCCAACCCGCC
CGAAACCGCGATGGCATACTTTGAGGCGATCCCGCGCAGGTGGTCGCTTCATCCCGACCAGTCGCGTGTTTCACCGATTA
TCGGTTTGAAAGAGGGCCGCGAGCGCGCGCTTTCTGCCTACAAAGAAAGCCGCAATCAGAGCTGA

Upstream 100 bases:

>100_bases
GAGTTTTACTCACTATACCGATCAATTGGAGGTGACAGCGCTGATCGAGGCCCTAGATCGCGTCCTGTAAAGACTGATCG
AGCAAGGGATAAAACTGACC

Downstream 100 bases:

>100_bases
GCCACGCGGTCGTGGCGATGGGTACACAGGTTGTCTTTGGGGCGGTGTCTGCTGTGGGTTTGCGCTGGATTTCTGCGCGA
CCTGCGCATAGCCTCGCCGA

Product: deoxyribodipyrimidine photo-lyase type I

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MADTRPVIWWIRRDLRLRDNPALRAAQEAGGPIIPLYIHDAQEEALGAAPKFRLGLGLERFAKTLEEKGSRLIVRRGDAL
EVLREVIAESGAGHVIWSRLYDPAATKRDAKIKEALKASDIKAKSTGGRLLFEPWTVDTKDGGMYRVYTPFWKAVRTRDV
AELTAAPSRLAAPESWPSGEALADLGMDAAMRRGGAIVAQHCRVGEDAALAQLDDFLTERVSNYKAHRDFPARAATSELS
ENLAWGEISPHRMWHLGAQAMQDGQPGAEHFLKEVVWREFAYHLMHRSPRILTRNWREEWDGFPWQDDPGDALQRWQQGQ
TGYDFVDAAMRELYVTGKMHNRARMIVASFLTKHLMIHWKYGLQWFEDCLVDWDPASNAMGWQWVAGSGPDAAPFFRIFN
PDGQLEKFDPKGDYARRWLPEGQANPPETAMAYFEAIPRRWSLHPDQSRVSPIIGLKEGRERALSAYKESRNQS

Sequences:

>Translated_474_residues
MADTRPVIWWIRRDLRLRDNPALRAAQEAGGPIIPLYIHDAQEEALGAAPKFRLGLGLERFAKTLEEKGSRLIVRRGDAL
EVLREVIAESGAGHVIWSRLYDPAATKRDAKIKEALKASDIKAKSTGGRLLFEPWTVDTKDGGMYRVYTPFWKAVRTRDV
AELTAAPSRLAAPESWPSGEALADLGMDAAMRRGGAIVAQHCRVGEDAALAQLDDFLTERVSNYKAHRDFPARAATSELS
ENLAWGEISPHRMWHLGAQAMQDGQPGAEHFLKEVVWREFAYHLMHRSPRILTRNWREEWDGFPWQDDPGDALQRWQQGQ
TGYDFVDAAMRELYVTGKMHNRARMIVASFLTKHLMIHWKYGLQWFEDCLVDWDPASNAMGWQWVAGSGPDAAPFFRIFN
PDGQLEKFDPKGDYARRWLPEGQANPPETAMAYFEAIPRRWSLHPDQSRVSPIIGLKEGRERALSAYKESRNQS
>Mature_473_residues
ADTRPVIWWIRRDLRLRDNPALRAAQEAGGPIIPLYIHDAQEEALGAAPKFRLGLGLERFAKTLEEKGSRLIVRRGDALE
VLREVIAESGAGHVIWSRLYDPAATKRDAKIKEALKASDIKAKSTGGRLLFEPWTVDTKDGGMYRVYTPFWKAVRTRDVA
ELTAAPSRLAAPESWPSGEALADLGMDAAMRRGGAIVAQHCRVGEDAALAQLDDFLTERVSNYKAHRDFPARAATSELSE
NLAWGEISPHRMWHLGAQAMQDGQPGAEHFLKEVVWREFAYHLMHRSPRILTRNWREEWDGFPWQDDPGDALQRWQQGQT
GYDFVDAAMRELYVTGKMHNRARMIVASFLTKHLMIHWKYGLQWFEDCLVDWDPASNAMGWQWVAGSGPDAAPFFRIFNP
DGQLEKFDPKGDYARRWLPEGQANPPETAMAYFEAIPRRWSLHPDQSRVSPIIGLKEGRERALSAYKESRNQS

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=451, Percent_Identity=29.2682926829268, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI188536103, Length=393, Percent_Identity=28.7531806615776, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI4758072, Length=442, Percent_Identity=27.8280542986425, Blast_Score=126, Evalue=4e-29,
Organism=Escherichia coli, GI1786926, Length=479, Percent_Identity=37.160751565762, Blast_Score=255, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6324962, Length=511, Percent_Identity=27.3972602739726, Blast_Score=183, Evalue=5e-47,
Organism=Drosophila melanogaster, GI17137248, Length=449, Percent_Identity=28.7305122494432, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24585455, Length=449, Percent_Identity=28.7305122494432, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24648152, Length=474, Percent_Identity=25.5274261603376, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 53840; Mature: 53709

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTRPVIWWIRRDLRLRDNPALRAAQEAGGPIIPLYIHDAQEEALGAAPKFRLGLGLER
CCCCCCEEHEEHHCCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHCCCCCHHHCCCHHH
FAKTLEEKGSRLIVRRGDALEVLREVIAESGAGHVIWSRLYDPAATKRDAKIKEALKASD
HHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHC
IKAKSTGGRLLFEPWTVDTKDGGMYRVYTPFWKAVRTRDVAELTAAPSRLAAPESWPSGE
CCCCCCCCEEEECCCEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCH
ALADLGMDAAMRRGGAIVAQHCRVGEDAALAQLDDFLTERVSNYKAHRDFPARAATSELS
HHHHHCHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHH
ENLAWGEISPHRMWHLGAQAMQDGQPGAEHFLKEVVWREFAYHLMHRSPRILTRNWREEW
HCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DGFPWQDDPGDALQRWQQGQTGYDFVDAAMRELYVTGKMHNRARMIVASFLTKHLMIHWK
CCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YGLQWFEDCLVDWDPASNAMGWQWVAGSGPDAAPFFRIFNPDGQLEKFDPKGDYARRWLP
HHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHCCC
EGQANPPETAMAYFEAIPRRWSLHPDQSRVSPIIGLKEGRERALSAYKESRNQS
CCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCHHHCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ADTRPVIWWIRRDLRLRDNPALRAAQEAGGPIIPLYIHDAQEEALGAAPKFRLGLGLER
CCCCCEEHEEHHCCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHCCCCCHHHCCCHHH
FAKTLEEKGSRLIVRRGDALEVLREVIAESGAGHVIWSRLYDPAATKRDAKIKEALKASD
HHHHHHHCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHC
IKAKSTGGRLLFEPWTVDTKDGGMYRVYTPFWKAVRTRDVAELTAAPSRLAAPESWPSGE
CCCCCCCCEEEECCCEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCH
ALADLGMDAAMRRGGAIVAQHCRVGEDAALAQLDDFLTERVSNYKAHRDFPARAATSELS
HHHHHCHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHH
ENLAWGEISPHRMWHLGAQAMQDGQPGAEHFLKEVVWREFAYHLMHRSPRILTRNWREEW
HCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
DGFPWQDDPGDALQRWQQGQTGYDFVDAAMRELYVTGKMHNRARMIVASFLTKHLMIHWK
CCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
YGLQWFEDCLVDWDPASNAMGWQWVAGSGPDAAPFFRIFNPDGQLEKFDPKGDYARRWLP
HHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHCCC
EGQANPPETAMAYFEAIPRRWSLHPDQSRVSPIIGLKEGRERALSAYKESRNQS
CCCCCCHHHHHHHHHHHCCCCCCCCCHHHCCHHHCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]