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The map label for this gene is miaA

Identifier: 99081256

GI number: 99081256

Start: 1508184

End: 1509050

Strand: Direct

Name: miaA

Synonym: TM1040_1415

Alternate gene names: 99081256

Gene position: 1508184-1509050 (Clockwise)

Preceding gene: 99081242

Following gene: 99081257

Centisome position: 47.12

GC content: 62.63

Gene sequence:

>867_bases
TTGTTGGATATTGATAAAATCCCAGCTGATCAGCCCGTGCTGATTGCCGGTCCAACCGCATCTGGCAAATCCGAGCTGGC
GCTGCGCATCGCCGAACACAGCGGTGGCGTGATCGTCAATGCCGACGCAAGCCAGGTCTATGACTGCTGGCGGGTTGTGA
CCGCGCGCCCCTCGGCAGAAGACGAATCCCGCGCAGCGCACGCGCTTTATGGTCATCTGAGCTATGACACACTTTATTCC
GCCGGGCACTGGCTGCGCGATGTCACACCGCTCTTGAAGGGCACGAAGCGCCCCATCATTGTTGGGGGCACCGGGCTTTA
TTTTCTGACACTCACAGAGGGGATGGCAGATATTCCCGCAACCCCGCCCGAAGTGCGCGCCGAAGCGGATACTCTGTCGC
TCGAAACGCTCGCCGCAGATCTCGATCCAGAGACGCGCGCCCGCATCGACATGCAGAACCGCGCGCGTGTGCAACGCGCC
TGGGAGGTATTGCGCGCCACCGGACGCCCGCTCGCCGAATGGCAGGATGACACGCCGCCACCGCTCTTGCCTGCCAAGGA
TTGCACCGCGCTGGTGGTCGAAGCAGAGCGCGACTGGCTCGAAACGCGCATTCGGCGTCGGTTTTCTATGATGATTGCCC
AAGGCGCGCTCGAGGAGGCGCGCGCCATGCAGGACAGGTATGATCCCGCGCTTCCCTCCTGCAAAGCGATCGGCGTCCCG
GAGCTGATGGCGCATCTGAGTGGCGCACTCACCCTAGAGCAAGCAGAGGAACGCGCCTCAGTTGCGACCCGGCAATATGC
CAAGCGGCAGCGCACTTGGTTCCGCGCCCGAATGAAATCTTGGCAGAGGGTCTCGCCGCAGGCATGA

Upstream 100 bases:

>100_bases
AACCCGCCGAGGATCAATCCGGCCAGCCGGATAATTGCCGATCTTCTTAAGTAGAGCGACCTCATCGCGGCAACGGCAAG
CAAAGGCGCAGGGCAAAACC

Downstream 100 bases:

>100_bases
TCTGAGCCTAGACAATCTGAACCCGCTTTCGCGCGGCGTGATCCAAAGCTTGCACAGAATTCAGCAGCCCGGCGCACACT
TTTCCGCCTGCTCTTTTCCG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MLDIDKIPADQPVLIAGPTASGKSELALRIAEHSGGVIVNADASQVYDCWRVVTARPSAEDESRAAHALYGHLSYDTLYS
AGHWLRDVTPLLKGTKRPIIVGGTGLYFLTLTEGMADIPATPPEVRAEADTLSLETLAADLDPETRARIDMQNRARVQRA
WEVLRATGRPLAEWQDDTPPPLLPAKDCTALVVEAERDWLETRIRRRFSMMIAQGALEEARAMQDRYDPALPSCKAIGVP
ELMAHLSGALTLEQAEERASVATRQYAKRQRTWFRARMKSWQRVSPQA

Sequences:

>Translated_288_residues
MLDIDKIPADQPVLIAGPTASGKSELALRIAEHSGGVIVNADASQVYDCWRVVTARPSAEDESRAAHALYGHLSYDTLYS
AGHWLRDVTPLLKGTKRPIIVGGTGLYFLTLTEGMADIPATPPEVRAEADTLSLETLAADLDPETRARIDMQNRARVQRA
WEVLRATGRPLAEWQDDTPPPLLPAKDCTALVVEAERDWLETRIRRRFSMMIAQGALEEARAMQDRYDPALPSCKAIGVP
ELMAHLSGALTLEQAEERASVATRQYAKRQRTWFRARMKSWQRVSPQA
>Mature_288_residues
MLDIDKIPADQPVLIAGPTASGKSELALRIAEHSGGVIVNADASQVYDCWRVVTARPSAEDESRAAHALYGHLSYDTLYS
AGHWLRDVTPLLKGTKRPIIVGGTGLYFLTLTEGMADIPATPPEVRAEADTLSLETLAADLDPETRARIDMQNRARVQRA
WEVLRATGRPLAEWQDDTPPPLLPAKDCTALVVEAERDWLETRIRRRFSMMIAQGALEEARAMQDRYDPALPSCKAIGVP
ELMAHLSGALTLEQAEERASVATRQYAKRQRTWFRARMKSWQRVSPQA

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=27.0096463022508, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1790613, Length=289, Percent_Identity=33.2179930795848, Blast_Score=138, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25144712, Length=307, Percent_Identity=24.4299674267101, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=25.6849315068493, Blast_Score=99, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24649830, Length=341, Percent_Identity=25.8064516129032, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_SILST (Q1GGR8)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613410.1
- ProteinModelPortal:   Q1GGR8
- SMR:   Q1GGR8
- STRING:   Q1GGR8
- GeneID:   4078045
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1415
- NMPDR:   fig|292414.1.peg.3481
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   SSP292414:TM1040_1415-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 31952; Mature: 31952

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIDKIPADQPVLIAGPTASGKSELALRIAEHSGGVIVNADASQVYDCWRVVTARPSAE
CCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCC
DESRAAHALYGHLSYDTLYSAGHWLRDVTPLLKGTKRPIIVGGTGLYFLTLTEGMADIPA
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEECCCCCCCC
TPPEVRAEADTLSLETLAADLDPETRARIDMQNRARVQRAWEVLRATGRPLAEWQDDTPP
CCHHHHCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCC
PLLPAKDCTALVVEAERDWLETRIRRRFSMMIAQGALEEARAMQDRYDPALPSCKAIGVP
CCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
ELMAHLSGALTLEQAEERASVATRQYAKRQRTWFRARMKSWQRVSPQA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLDIDKIPADQPVLIAGPTASGKSELALRIAEHSGGVIVNADASQVYDCWRVVTARPSAE
CCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCEEEECCHHHHHHHHHHHHCCCCCC
DESRAAHALYGHLSYDTLYSAGHWLRDVTPLLKGTKRPIIVGGTGLYFLTLTEGMADIPA
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEECCCCCCCC
TPPEVRAEADTLSLETLAADLDPETRARIDMQNRARVQRAWEVLRATGRPLAEWQDDTPP
CCHHHHCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHCCCCCCC
PLLPAKDCTALVVEAERDWLETRIRRRFSMMIAQGALEEARAMQDRYDPALPSCKAIGVP
CCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
ELMAHLSGALTLEQAEERASVATRQYAKRQRTWFRARMKSWQRVSPQA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA