Click here to switch to the map view.

The map label for this gene is rseP [H]

Identifier: 99081250

GI number: 99081250

Start: 1502394

End: 1503746

Strand: Reverse

Name: rseP [H]

Synonym: TM1040_1409

Alternate gene names: 99081250

Gene position: 1503746-1502394 (Counterclockwise)

Preceding gene: 99081251

Following gene: 99081249

Centisome position: 46.98

GC content: 61.05

Gene sequence:

>1353_bases
TTGGACGCAGTATTTTTGGTCTCGCAACTGGGCGGGCTCCTTTACACGATCGGCAGCTTTGTCGTGGTGCTTTCGATCAT
CGTGTTCGTGCATGAATACGGTCATTACATCGTGGGACGTTGGTGCGGCATTCATCCGGAGGTCTTCTCGCTGGGGTTTG
GCCCGGTGCTGGCCAGCCGGGTCGACAAGCGCGGCACCCGATGGCAGCTGGCGGCCTTTCCGTTTGGCGGGTTTGTGAAA
TTTCTGGGCGACGCGGACGCGGCCTCTGGCAAGGATGCCAGCGCGATTTCGGCGGCCGAGCGCGACCCTGAACTGCTGCG
CAAAACCATGCATGGCGCGCCGCTCTGGGCGCGGGCGGCAACCGTGGCGGCGGGTCCGATTTTCAACTTCGTCCTTGCGG
CAGTGATCTTCACCGGCGTCAATCTGAGCCGGGGCCAGATGCAGGAGCCTTTTGCAGTAGGCGAAATCAAGCCACTGCCC
GCAGAGAGCTACACCCTGCAGCCCGGTGACGAAATTCTGTCGGTCGCAGGGATCGTGACGCCAGATTTTTCTGACGCCGT
TGCTTGGGGCGCCTTCGAGGGCGGCCTGCCAGTGGAAAAGGTTCTGGAGTATCGCGTTGTGCGCGACGGTCAGGAAATGA
TCGCCCGCGGGCCCTATCTGACCCCAAGCCTCGTCTCCGGTGTGGCGCCGCGCAGCGCGGCATCTGACGCAGGCCTGCGC
GAAGGCGATGTGATCGTAGGGGTCGATGGTGAAGAGATCTTTGCCTTCTCCCACTTGAAGGAACGTGTCGAGACCGGTGC
GGGTGCGCCCTTGGAGTTGACCGTCTGGAACGCGGGCCAGACACGCGAGGTGACACTAACGCCACGCCGAACCGATGAAC
CGACAGCCGATGGCGGGTTCCAGACCAATTGGCGGATCGGGATCGCCGGCGGGCTTGCCTTTGAGCCTGCGCGCGAGTCG
GTCTCGCCTGTGGCCGCCGTCGGGCAGGGGGTGACACAGGTCTGGATCATGATTGAACAATCCCTTTCGGGTCTGAAACA
CATGATCACGGGTCAGATCAGCACCTGCAACCTCTCTGGCCCGGTTGCGATCGCCGAGATTTCCGGAACTCTGGCAAGCC
AGGGGGCGATGAATTTCATCTGGTTGATTGCGGCGCTGTCGACAGGGATTGGTCTTTTGAACCTTTTTCCAGTGCCGGTG
CTGGATGGCGGTCACCTGGTTTTCTTTGCCTATGAGGCCGTGACCGGGAAGCCCCCGAATGATCGCGCGATGCAGATCCT
GATGACCATCGGCCTGACCCTGATCCTTGGTCTGATGATCTTTTCCGTCAGCAACGATCTTTTGTTCTGCTGA

Upstream 100 bases:

>100_bases
CTTATTGATGCCTCAATGACACTTGAAAACGTCACCCGAGTTGACGATCTGGCGCGTAGGGCGGTGGATCAGGTCGTGAC
GCAACGAACAGGATAGAGTT

Downstream 100 bases:

>100_bases
GACTGCGCCATGGCGCTTGGGCGGGATGGGGCAGCCTTGCCTCGTTCCCGCCCAGATCTCGCCACAAATCTTTCCTAGCT
TCTCTTTGGGTGAGTGATTC

Product: peptidase RseP

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MDAVFLVSQLGGLLYTIGSFVVVLSIIVFVHEYGHYIVGRWCGIHPEVFSLGFGPVLASRVDKRGTRWQLAAFPFGGFVK
FLGDADAASGKDASAISAAERDPELLRKTMHGAPLWARAATVAAGPIFNFVLAAVIFTGVNLSRGQMQEPFAVGEIKPLP
AESYTLQPGDEILSVAGIVTPDFSDAVAWGAFEGGLPVEKVLEYRVVRDGQEMIARGPYLTPSLVSGVAPRSAASDAGLR
EGDVIVGVDGEEIFAFSHLKERVETGAGAPLELTVWNAGQTREVTLTPRRTDEPTADGGFQTNWRIGIAGGLAFEPARES
VSPVAAVGQGVTQVWIMIEQSLSGLKHMITGQISTCNLSGPVAIAEISGTLASQGAMNFIWLIAALSTGIGLLNLFPVPV
LDGGHLVFFAYEAVTGKPPNDRAMQILMTIGLTLILGLMIFSVSNDLLFC

Sequences:

>Translated_450_residues
MDAVFLVSQLGGLLYTIGSFVVVLSIIVFVHEYGHYIVGRWCGIHPEVFSLGFGPVLASRVDKRGTRWQLAAFPFGGFVK
FLGDADAASGKDASAISAAERDPELLRKTMHGAPLWARAATVAAGPIFNFVLAAVIFTGVNLSRGQMQEPFAVGEIKPLP
AESYTLQPGDEILSVAGIVTPDFSDAVAWGAFEGGLPVEKVLEYRVVRDGQEMIARGPYLTPSLVSGVAPRSAASDAGLR
EGDVIVGVDGEEIFAFSHLKERVETGAGAPLELTVWNAGQTREVTLTPRRTDEPTADGGFQTNWRIGIAGGLAFEPARES
VSPVAAVGQGVTQVWIMIEQSLSGLKHMITGQISTCNLSGPVAIAEISGTLASQGAMNFIWLIAALSTGIGLLNLFPVPV
LDGGHLVFFAYEAVTGKPPNDRAMQILMTIGLTLILGLMIFSVSNDLLFC
>Mature_450_residues
MDAVFLVSQLGGLLYTIGSFVVVLSIIVFVHEYGHYIVGRWCGIHPEVFSLGFGPVLASRVDKRGTRWQLAAFPFGGFVK
FLGDADAASGKDASAISAAERDPELLRKTMHGAPLWARAATVAAGPIFNFVLAAVIFTGVNLSRGQMQEPFAVGEIKPLP
AESYTLQPGDEILSVAGIVTPDFSDAVAWGAFEGGLPVEKVLEYRVVRDGQEMIARGPYLTPSLVSGVAPRSAASDAGLR
EGDVIVGVDGEEIFAFSHLKERVETGAGAPLELTVWNAGQTREVTLTPRRTDEPTADGGFQTNWRIGIAGGLAFEPARES
VSPVAAVGQGVTQVWIMIEQSLSGLKHMITGQISTCNLSGPVAIAEISGTLASQGAMNFIWLIAALSTGIGLLNLFPVPV
LDGGHLVFFAYEAVTGKPPNDRAMQILMTIGLTLILGLMIFSVSNDLLFC

Specific function: Cleaves the peptide bond between 'Ala-108' and 'Cys-109' in the transmembrane region of rseA. Acts on degS-cleaved rseA to release the cytoplasmic domain of rseA. This provides the cell with sigma E (rpoE) activity through the proteolysis of rseA. In vitr

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=448, Percent_Identity=32.3660714285714, Blast_Score=196, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 47687; Mature: 47687

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50106 PDZ ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVFLVSQLGGLLYTIGSFVVVLSIIVFVHEYGHYIVGRWCGIHPEVFSLGFGPVLASR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHCCCCHHHHHCCCHHHHHHH
VDKRGTRWQLAAFPFGGFVKFLGDADAASGKDASAISAAERDPELLRKTMHGAPLWARAA
HHCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHH
TVAAGPIFNFVLAAVIFTGVNLSRGQMQEPFAVGEIKPLPAESYTLQPGDEILSVAGIVT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHEEC
PDFSDAVAWGAFEGGLPVEKVLEYRVVRDGQEMIARGPYLTPSLVSGVAPRSAASDAGLR
CCCCHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHHCCCCCCCCCCCCCC
EGDVIVGVDGEEIFAFSHLKERVETGAGAPLELTVWNAGQTREVTLTPRRTDEPTADGGF
CCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCC
QTNWRIGIAGGLAFEPARESVSPVAAVGQGVTQVWIMIEQSLSGLKHMITGQISTCNLSG
CCCEEEEEECCCEECCCHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
PVAIAEISGTLASQGAMNFIWLIAALSTGIGLLNLFPVPVLDGGHLVFFAYEAVTGKPPN
CEEEEECCCHHHHCCHHHHHHHHHHHHHCCCCHHHCCCCEECCCEEEEEEEEHHCCCCCC
DRAMQILMTIGLTLILGLMIFSVSNDLLFC
HHHHHHHHHHHHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MDAVFLVSQLGGLLYTIGSFVVVLSIIVFVHEYGHYIVGRWCGIHPEVFSLGFGPVLASR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEHHCCCCHHHHHCCCHHHHHHH
VDKRGTRWQLAAFPFGGFVKFLGDADAASGKDASAISAAERDPELLRKTMHGAPLWARAA
HHCCCCEEEEEECCCCHHHHHHCCCCCCCCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHH
TVAAGPIFNFVLAAVIFTGVNLSRGQMQEPFAVGEIKPLPAESYTLQPGDEILSVAGIVT
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHEEC
PDFSDAVAWGAFEGGLPVEKVLEYRVVRDGQEMIARGPYLTPSLVSGVAPRSAASDAGLR
CCCCHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHHCCCCCCCCCCCCCC
EGDVIVGVDGEEIFAFSHLKERVETGAGAPLELTVWNAGQTREVTLTPRRTDEPTADGGF
CCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCC
QTNWRIGIAGGLAFEPARESVSPVAAVGQGVTQVWIMIEQSLSGLKHMITGQISTCNLSG
CCCEEEEEECCCEECCCHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
PVAIAEISGTLASQGAMNFIWLIAALSTGIGLLNLFPVPVLDGGHLVFFAYEAVTGKPPN
CEEEEECCCHHHHCCHHHHHHHHHHHHHCCCCHHHCCCCEECCCEEEEEEEEHHCCCCCC
DRAMQILMTIGLTLILGLMIFSVSNDLLFC
HHHHHHHHHHHHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]