Click here to switch to the map view.

The map label for this gene is hisI

Identifier: 99081235

GI number: 99081235

Start: 1487246

End: 1487635

Strand: Reverse

Name: hisI

Synonym: TM1040_1394

Alternate gene names: 99081235

Gene position: 1487635-1487246 (Counterclockwise)

Preceding gene: 99081238

Following gene: 99081231

Centisome position: 46.47

GC content: 56.41

Gene sequence:

>390_bases
ATGCAAAAGGTTTTTAACATGCACGATTTGGTGAAGTTCTCGCCCGAGACGCTCAAATACAACGAAGCTGGCCTTGTGCC
CTGTATTGCTCAGGATGTGGAAAGCGGCGAGATTCTGATGATGGCCTGGATGAACGCGGAGAGCGTCGCCAAAACGCTCG
AGACCGGGCGGGTGACCTATTGGTCGCGCTCGCGCCAGTCGTTCTGGATCAAGGGGGAGAGTTCGGGCCATGTGCAGGAA
CTGGTCGAACTGCGCGTCGATTGCGACCGTGATGCGCTTTTGGCAATGGTGCGTCAGACCGGGCCTGCTTGCCACACCAA
TCGCCGCAGCTGTTTCTACACGGCTATTCGGGATGGCGAAGAGCATGAGATCATGACGCCAATATCCTGA

Upstream 100 bases:

>100_bases
TTGGTGGAGTAGAGCTTGATCAGATCGGTTTCGCCGGACATGTCGTGTTCCTGGGTTTCCCTTGCACCGTGTTCCTCATA
CATAGGGGCGTGCCCCTGAG

Downstream 100 bases:

>100_bases
TCTAAAATGCTAATCTAGCCCGACGAGTTGTCTGATATCTTGCGGGCTGGCGCCCTCGGAACGGTATTTTGAAATGGCGC
GGGCATCATCGCGTTTGGCA

Product: phosphoribosyl-AMP cyclohydrolase

Products: NA

Alternate protein names: PRA-CH

Number of amino acids: Translated: 129; Mature: 129

Protein sequence:

>129_residues
MQKVFNMHDLVKFSPETLKYNEAGLVPCIAQDVESGEILMMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQE
LVELRVDCDRDALLAMVRQTGPACHTNRRSCFYTAIRDGEEHEIMTPIS

Sequences:

>Translated_129_residues
MQKVFNMHDLVKFSPETLKYNEAGLVPCIAQDVESGEILMMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQE
LVELRVDCDRDALLAMVRQTGPACHTNRRSCFYTAIRDGEEHEIMTPIS
>Mature_129_residues
MQKVFNMHDLVKFSPETLKYNEAGLVPCIAQDVESGEILMMAWMNAESVAKTLETGRVTYWSRSRQSFWIKGESSGHVQE
LVELRVDCDRDALLAMVRQTGPACHTNRRSCFYTAIRDGEEHEIMTPIS

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PRA-CH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=89, Percent_Identity=48.314606741573, Blast_Score=103, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319819, Length=75, Percent_Identity=44, Blast_Score=74, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS3_SILST (Q1GGT9)

Other databases:

- EMBL:   CP000377
- RefSeq:   YP_613389.1
- ProteinModelPortal:   Q1GGT9
- SMR:   Q1GGT9
- STRING:   Q1GGT9
- GeneID:   4075887
- GenomeReviews:   CP000377_GR
- KEGG:   sit:TM1040_1394
- NMPDR:   fig|292414.1.peg.3500
- eggNOG:   COG0139
- HOGENOM:   HBG294308
- OMA:   LWLKGES
- ProtClustDB:   CLSK933610
- BioCyc:   SSP292414:TM1040_1394-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01021
- InterPro:   IPR002496
- ProDom:   PD002610

Pfam domain/function: PF01502 PRA-CH

EC number: =3.5.4.19

Molecular weight: Translated: 14714; Mature: 14714

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKVFNMHDLVKFSPETLKYNEAGLVPCIAQDVESGEILMMAWMNAESVAKTLETGRVTY
CCCCCCHHHHHHCCCCCEEECCCCCCHHHHCCCCCCCEEEEEECCHHHHHHHHHCCCEEE
WSRSRQSFWIKGESSGHVQELVELRVDCDRDALLAMVRQTGPACHTNRRSCFYTAIRDGE
EECCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
EHEIMTPIS
CCCEECCCC
>Mature Secondary Structure
MQKVFNMHDLVKFSPETLKYNEAGLVPCIAQDVESGEILMMAWMNAESVAKTLETGRVTY
CCCCCCHHHHHHCCCCCEEECCCCCCHHHHCCCCCCCEEEEEECCHHHHHHHHHCCCEEE
WSRSRQSFWIKGESSGHVQELVELRVDCDRDALLAMVRQTGPACHTNRRSCFYTAIRDGE
EECCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
EHEIMTPIS
CCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA