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The map label for this gene is gluQ [H]

Identifier: 99081234

GI number: 99081234

Start: 1486388

End: 1487236

Strand: Direct

Name: gluQ [H]

Synonym: TM1040_1393

Alternate gene names: 99081234

Gene position: 1486388-1487236 (Clockwise)

Preceding gene: 99081233

Following gene: 99081236

Centisome position: 46.44

GC content: 60.42

Gene sequence:

>849_bases
GTGACATTCACAACTCGTTTTGCCCCTTCTCCGACGGGGCCTCTGCACTTGGGCCATGCCTATTCCGCGTTGCTCGCTTC
TGATTTTGCGCGCCAGCAGAACGGTCGATTTCTGCTGAGGATCGAAGACATCGATCAGACGCGCGCCCGAGCCTCTTGGG
AAGAGCAGATCTATCAGGATCTTGCCTGGCTTGGTCTCAGTTGGCCGGAGCCGGTCTTGCGCCAATCTTCACGCTTGCCC
CGATACCGCGCCGCGCTGGAACGCCTGAGCGCATTGGGGCTGACCTATCCCTGTCGCTGCAATCGCGCCGATATTGAGGC
CGCGGCAGGCGCACCTCAGGAGGGTGTGCCGCAGTTTGGCCCCGACGGGCGCATCTACCCCGGCACATGCCGCCAGCGAC
CGCTCTCGGATGCACGCCCAACCGACGTGATCCGCCTCAATATGAAGGCGGCGCTCGCACGGCTCCCAGCCCTGAGTTTT
ATTGAGACATCCGAGGAACTTGGCGGCCATATCTCGCTTGATCCCGAAGAACTTGTGCAAGCAGTCGGCGATATCATCCT
GGTGCGCCGCAATATGGGCAGTTCCTATCACCTCTCGGTGGTGGTGGATGACGCCGATCAGGGCATCACGGATGTGGTGC
GCGGTGCGGATCTCTTTGAAAGCACGCAGATCCATGTGGTGCTGCAGACGCTTCTGGATCTGCCAGTCCCGCGCTACCAT
CATCACCGCCTGATCCGGGACGATCAGGGCAAGAGACTTGCCAAACGCGATGATGCCCGCGCCATTTCAAAATACCGTTC
CGAGGGCGCCAGCCCGCAAGATATCAGACAACTCGTCGGGCTAGATTAG

Upstream 100 bases:

>100_bases
AATGGCTTGATTGCGGGGCTGCGCGGTTGCTATTCAAGGAACATGGCCCCCACCTGCCGGGCCTCAACCTGAACGCCTGC
GTCTATGTGATTGAAAAAGT

Downstream 100 bases:

>100_bases
CATTTTAGATCAGGATATTGGCGTCATGATCTCATGCTCTTCGCCATCCCGAATAGCCGTGTAGAAACAGCTGCGGCGAT
TGGTGTGGCAAGCAGGCCCG

Product: glutamyl-tRNA synthetase, class Ic

Products: AMP; L-Glutamyl-tRNA(Glu); Diphosphate [C]

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTFTTRFAPSPTGPLHLGHAYSALLASDFARQQNGRFLLRIEDIDQTRARASWEEQIYQDLAWLGLSWPEPVLRQSSRLP
RYRAALERLSALGLTYPCRCNRADIEAAAGAPQEGVPQFGPDGRIYPGTCRQRPLSDARPTDVIRLNMKAALARLPALSF
IETSEELGGHISLDPEELVQAVGDIILVRRNMGSSYHLSVVVDDADQGITDVVRGADLFESTQIHVVLQTLLDLPVPRYH
HHRLIRDDQGKRLAKRDDARAISKYRSEGASPQDIRQLVGLD

Sequences:

>Translated_282_residues
MTFTTRFAPSPTGPLHLGHAYSALLASDFARQQNGRFLLRIEDIDQTRARASWEEQIYQDLAWLGLSWPEPVLRQSSRLP
RYRAALERLSALGLTYPCRCNRADIEAAAGAPQEGVPQFGPDGRIYPGTCRQRPLSDARPTDVIRLNMKAALARLPALSF
IETSEELGGHISLDPEELVQAVGDIILVRRNMGSSYHLSVVVDDADQGITDVVRGADLFESTQIHVVLQTLLDLPVPRYH
HHRLIRDDQGKRLAKRDDARAISKYRSEGASPQDIRQLVGLD
>Mature_281_residues
TFTTRFAPSPTGPLHLGHAYSALLASDFARQQNGRFLLRIEDIDQTRARASWEEQIYQDLAWLGLSWPEPVLRQSSRLPR
YRAALERLSALGLTYPCRCNRADIEAAAGAPQEGVPQFGPDGRIYPGTCRQRPLSDARPTDVIRLNMKAALARLPALSFI
ETSEELGGHISLDPEELVQAVGDIILVRRNMGSSYHLSVVVDDADQGITDVVRGADLFESTQIHVVLQTLLDLPVPRYHH
HRLIRDDQGKRLAKRDDARAISKYRSEGASPQDIRQLVGLD

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=286, Percent_Identity=29.3706293706294, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI1788743, Length=276, Percent_Identity=35.8695652173913, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI226510925, Length=262, Percent_Identity=37.4045801526718, Blast_Score=136, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71993394, Length=293, Percent_Identity=30.3754266211604, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324540, Length=286, Percent_Identity=32.5174825174825, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324742, Length=113, Percent_Identity=29.2035398230088, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24665321, Length=286, Percent_Identity=31.4685314685315, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17 [C]

Molecular weight: Translated: 31479; Mature: 31348

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFTTRFAPSPTGPLHLGHAYSALLASDFARQQNGRFLLRIEDIDQTRARASWEEQIYQD
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHH
LAWLGLSWPEPVLRQSSRLPRYRAALERLSALGLTYPCRCNRADIEAAAGAPQEGVPQFG
HHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCC
PDGRIYPGTCRQRPLSDARPTDVIRLNMKAALARLPALSFIETSEELGGHISLDPEELVQ
CCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHH
AVGDIILVRRNMGSSYHLSVVVDDADQGITDVVRGADLFESTQIHVVLQTLLDLPVPRYH
HHHHHEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHH
HHRLIRDDQGKRLAKRDDARAISKYRSEGASPQDIRQLVGLD
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TFTTRFAPSPTGPLHLGHAYSALLASDFARQQNGRFLLRIEDIDQTRARASWEEQIYQD
CCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHH
LAWLGLSWPEPVLRQSSRLPRYRAALERLSALGLTYPCRCNRADIEAAAGAPQEGVPQFG
HHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCCC
PDGRIYPGTCRQRPLSDARPTDVIRLNMKAALARLPALSFIETSEELGGHISLDPEELVQ
CCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHH
AVGDIILVRRNMGSSYHLSVVVDDADQGITDVVRGADLFESTQIHVVLQTLLDLPVPRYH
HHHHHEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHH
HHRLIRDDQGKRLAKRDDARAISKYRSEGASPQDIRQLVGLD
HHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-Glutamate; tRNA (Glu) [C]

Specific reaction: ATP + L-Glutamate + tRNA (Glu) --> AMP + L-Glutamyl-tRNA(Glu) + Diphosphate [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA